We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme, isolated from the bacterium Streptomyces galilaeus, is involved in the biosynthesis of other aclacinomycins. Also acts on idarubicin. It will slowly add a second 2-deoxy-L-fucose unit to aclacinomycin S in vitro.
The enzyme appears in viruses and cellular organisms
Synonyms
AknK, L-2-deoxyfucosyltransferase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-2-deoxyfucosyltransferase
AknK
-
-
-
-
L-2-deoxyfucosyltransferase
-
-
L-2-deoxyfucosyltransferase
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T = dTDP + aclacinomycin S
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dTDP-2-deoxy-beta-L-fucose:7-(alpha-L-rhodosaminyl)aklavinone 2-deoxy-alpha-L-fucosyltransferase
The enzyme, isolated from the bacterium Streptomyces galilaeus, is involved in the biosynthesis of other aclacinomycins. Also acts on idarubicin. It will slowly add a second 2-deoxy-L-fucose unit to aclacinomycin S in vitro.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
dTDP-L-2-deoxyfucose + aclacinomycin S
dTDP + 2-deoxy-alpha-D-fucosyl-aclacinomyin S
dTDP-L-2-deoxyfucose + aclacinomycin T
dTDP + aclacinomycin S
idarubicin + dTDP-L-2-deoxyfucose
dTDP + 4'-O-(alpha-L-fucosyl)idarubicin
rhodosaminyl aklavinone + dTDP-L-daunosamine
dTDP + daunosaminyl-rhodosaminyl-aklavinone
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
-
-
-
-
?
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
-
-
-
-
?
dTDP-L-2-deoxyfucose + aclacinomycin S
dTDP + 2-deoxy-alpha-D-fucosyl-aclacinomyin S
-
-
-
-
?
dTDP-L-2-deoxyfucose + aclacinomycin S
dTDP + 2-deoxy-alpha-D-fucosyl-aclacinomyin S
-
-
-
-
?
dTDP-L-2-deoxyfucose + aclacinomycin T
dTDP + aclacinomycin S
-
-
-
-
?
dTDP-L-2-deoxyfucose + aclacinomycin T
dTDP + aclacinomycin S
-
-
-
-
?
idarubicin + dTDP-L-2-deoxyfucose
dTDP + 4'-O-(alpha-L-fucosyl)idarubicin
-
-
-
-
?
idarubicin + dTDP-L-2-deoxyfucose
dTDP + 4'-O-(alpha-L-fucosyl)idarubicin
-
-
-
-
?
rhodosaminyl aklavinone + dTDP-L-daunosamine
dTDP + daunosaminyl-rhodosaminyl-aklavinone
-
-
-
-
?
rhodosaminyl aklavinone + dTDP-L-daunosamine
dTDP + daunosaminyl-rhodosaminyl-aklavinone
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
-
-
-
-
?
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.1489 - 0.531
dTDP-L-2-deoxyfucose
0.94
dTDP-L-daunosamine
-
in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
0.138
idarubicin
-
in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
0.1044 - 0.1091
rhodosaminyl aklavinone
0.1489
dTDP-L-2-deoxyfucose
-
with rhodosaminyl aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
0.531
dTDP-L-2-deoxyfucose
-
with idarubicin as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
0.1044
rhodosaminyl aklavinone
-
with TDP-L-daunosamine as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
0.1091
rhodosaminyl aklavinone
-
with TDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.09
idarubicin
-
in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
0.035 - 1.09
rhodosaminyl aklavinone
0.035
rhodosaminyl aklavinone
-
with TDP-L-daunosamine as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
1.09
rhodosaminyl aklavinone
-
with TDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
AKNK_STRGJ
440
0
49164
Swiss-Prot
-
A0A7X0N6I7_9ACTN
428
0
46064
TrEMBL
-
A0A385ZBP2_9ACTN
445
0
47389
TrEMBL
-
A0A7X0N6M3_9ACTN
420
0
45316
TrEMBL
-
A0A7W4ZTL2_9ACTN
430
0
47443
TrEMBL
-
A0A7X0KFR3_9ACTN
435
0
47472
TrEMBL
-
A0A7X0KE38_9ACTN
429
0
47107
TrEMBL
-
A0A7X0KFP9_9ACTN
420
0
44960
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
50685
-
x * 50685, calculated from amino acid sequence
50699
-
x * 50699, MALDI-TOF mass spectrometry
65000
-
x * 65000, His6-tagged enyzme, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 50685, calculated from amino acid sequence
?
-
x * 50699, MALDI-TOF mass spectrometry
?
-
x * 65000, His6-tagged enyzme, SDS-PAGE
?
-
x * 50685, calculated from amino acid sequence
-
?
-
x * 50699, MALDI-TOF mass spectrometry
-
?
-
x * 65000, His6-tagged enyzme, SDS-PAGE
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ni-NTA resin column chromatography
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21(DE3) cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Lu, W.; Leimkuhler, C.; Oberthür, M.; Kahne, D.; Walsh, C.T.
AknK is an L-2-deoxyfucosyltransferase in the biosynthesis of the anthracycline aclacinomycin A
Biochemistry
43
4548-4558
2004
Streptomyces galilaeus, Streptomyces galilaeus ATCC 31615
brenda
Select items on the left to see more content.
html completed
BRENDA home
History
2.4.1.327 aclacinomycin-T 2-deoxy-L-fucose transferase
more
top
