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Information on EC 2.4.1.327 - aclacinomycin-T 2-deoxy-L-fucose transferase for references in articles please use BRENDA:EC2.4.1.327
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EC Tree
IUBMB Comments The enzyme, isolated from the bacterium Streptomyces galilaeus, is involved in the biosynthesis of other aclacinomycins. Also acts on idarubicin. It will slowly add a second 2-deoxy-L-fucose unit to aclacinomycin S in vitro.
The expected taxonomic range for this enzyme is: Streptomyces galilaeus
Synonyms
AknK, L-2-deoxyfucosyltransferase,
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L-2-deoxyfucosyltransferase
AknK
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L-2-deoxyfucosyltransferase
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L-2-deoxyfucosyltransferase
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dTDP-2-deoxy-beta-L-fucose + aclacinomycin T = dTDP + aclacinomycin S
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dTDP-2-deoxy-beta-L-fucose:7-(alpha-L-rhodosaminyl)aklavinone 2-deoxy-alpha-L-fucosyltransferase
The enzyme, isolated from the bacterium Streptomyces galilaeus, is involved in the biosynthesis of other aclacinomycins. Also acts on idarubicin. It will slowly add a second 2-deoxy-L-fucose unit to aclacinomycin S in vitro.
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dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
dTDP-L-2-deoxyfucose + aclacinomycin S
dTDP + 2-deoxy-alpha-D-fucosyl-aclacinomyin S
dTDP-L-2-deoxyfucose + aclacinomycin T
dTDP + aclacinomycin S
idarubicin + dTDP-L-2-deoxyfucose
dTDP + 4'-O-(alpha-L-fucosyl)idarubicin
rhodosaminyl aklavinone + dTDP-L-daunosamine
dTDP + daunosaminyl-rhodosaminyl-aklavinone
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
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?
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
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?
dTDP-L-2-deoxyfucose + aclacinomycin S
dTDP + 2-deoxy-alpha-D-fucosyl-aclacinomyin S
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?
dTDP-L-2-deoxyfucose + aclacinomycin S
dTDP + 2-deoxy-alpha-D-fucosyl-aclacinomyin S
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?
dTDP-L-2-deoxyfucose + aclacinomycin T
dTDP + aclacinomycin S
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?
dTDP-L-2-deoxyfucose + aclacinomycin T
dTDP + aclacinomycin S
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?
idarubicin + dTDP-L-2-deoxyfucose
dTDP + 4'-O-(alpha-L-fucosyl)idarubicin
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?
idarubicin + dTDP-L-2-deoxyfucose
dTDP + 4'-O-(alpha-L-fucosyl)idarubicin
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?
rhodosaminyl aklavinone + dTDP-L-daunosamine
dTDP + daunosaminyl-rhodosaminyl-aklavinone
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?
rhodosaminyl aklavinone + dTDP-L-daunosamine
dTDP + daunosaminyl-rhodosaminyl-aklavinone
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dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
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?
dTDP-2-deoxy-beta-L-fucose + aclacinomycin T
dTDP + aclacinomycin S
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0.1489 - 0.531
dTDP-L-2-deoxyfucose
0.94
dTDP-L-daunosamine
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in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
0.138
idarubicin
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in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
0.1044 - 0.1091
rhodosaminyl aklavinone
0.1489
dTDP-L-2-deoxyfucose
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with rhodosaminyl aklavinone as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
0.531
dTDP-L-2-deoxyfucose
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with idarubicin as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
0.1044
rhodosaminyl aklavinone
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with TDP-L-daunosamine as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
0.1091
rhodosaminyl aklavinone
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with TDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
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0.09
idarubicin
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in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
0.035 - 1.09
rhodosaminyl aklavinone
0.035
rhodosaminyl aklavinone
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with TDP-L-daunosamine as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
1.09
rhodosaminyl aklavinone
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with TDP-L-2-deoxyfucose as cosubstrate, in 75 mM Tris (pH 7.5), 12 mM MgCl2, at 25°C
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brenda
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brenda
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AKNK_STRGJ
440
0
49164
Swiss-Prot
A0A385ZBP2_9ACTN
445
0
47389
TrEMBL
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50685
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x * 50685, calculated from amino acid sequence
50699
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x * 50699, MALDI-TOF mass spectrometry
65000
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x * 65000, His6-tagged enyzme, SDS-PAGE
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?
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x * 50685, calculated from amino acid sequence; x * 50699, MALDI-TOF mass spectrometry; x * 65000, His6-tagged enyzme, SDS-PAGE
?
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x * 50685, calculated from amino acid sequence; x * 50699, MALDI-TOF mass spectrometry; x * 65000, His6-tagged enyzme, SDS-PAGE
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Ni-NTA resin column chromatography
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expressed in Escherichia coli BL21(DE3) cells
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Lu, W.; Leimkuhler, C.; Oberthür, M.; Kahne, D.; Walsh, C.T.
AknK is an L-2-deoxyfucosyltransferase in the biosynthesis of the anthracycline aclacinomycin A
Biochemistry
43
4548-4558
2004
Streptomyces galilaeus, Streptomyces galilaeus ATCC 31615
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2.4.1.327 aclacinomycin-T 2-deoxy-L-fucose transferase
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