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Information on EC 2.4.1.306 - UDP-GalNAc:alpha-D-GalNAc-diphosphoundecaprenol alpha-1,3-N-acetylgalactosaminyltransferase
for references in articles please use BRENDA:EC2.4.1.306
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.306 UDP-GalNAc:alpha-D-GalNAc-diphosphoundecaprenol alpha-1,3-N-acetylgalactosaminyltransferase
IUBMB Comments
The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86.
The expected taxonomic range for this enzyme is: Escherichia coli
Reaction Schemes
showSynonyms
WbnH, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
WbnH
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
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PATHWAY SOURCE
PATHWAYS
BRENDA
MetaCyc
Escherichia coli serotype O:127 O antigen biosynthesis, Escherichia coli serotype O:86 O antigen biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-alpha-D-galactosamine:N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol alpha-1,3-N-acetyl-D-galactosyltransferase
The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-(CH2)10-CH3
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-(CH2)10-CH3
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-11-phenoxyundecanol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-11-phenoxyundecanol
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-(CH2)10-CH3
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-(CH2)10-CH3
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Substrates: 95% of the activity with UDP-N-acetyl-D-galactosamine + D-GalNAc-alpha-diphospho-11-phenoxyundecanol
Products: -
Products: -
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UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-(CH2)10-CH3
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-(CH2)10-CH3
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Substrates: 95% of the activity with UDP-N-acetyl-D-galactosamine + D-GalNAc-alpha-diphospho-11-phenoxyundecanol
Products: -
Products: -
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UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-11-phenoxyundecanol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-11-phenoxyundecanol
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Substrates: synthetic acceptor substrate. Diphosphate and hydrophobic lipid moiety are structural requirements for WbnH activity
Products: -
Products: -
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UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-11-phenoxyundecanol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-11-phenoxyundecanol
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Substrates: synthetic acceptor substrate. Diphosphate and hydrophobic lipid moiety are structural requirements for WbnH activity
Products: -
Products: -
?
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
Substrates: the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria
Products: -
Products: -
?
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
Q5J7D6
Substrates: biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI
Products: -
Products: -
?
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
Substrates: the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria
Products: -
Products: -
?
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
Q5J7D6
Substrates: biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI
Products: -
Products: -
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additional information
?
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Substrates: the natural substrate N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol is difficult to isolate from natural bacterial sources due to the low abundance. The highly hydrophobic feature of the 55-carbon undecaprenyl chain makes it hard to handle
Products: -
Products: -
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additional information
?
-
-
Substrates: the natural substrate N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol is difficult to isolate from natural bacterial sources due to the low abundance. The highly hydrophobic feature of the 55-carbon undecaprenyl chain makes it hard to handle
Products: -
Products: -
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
Substrates: the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria
Products: -
Products: -
?
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
Q5J7D6
Substrates: biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI
Products: -
Products: -
?
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
-
Substrates: the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria
Products: -
Products: -
?
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-alpha-D-galactosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
Q5J7D6
Substrates: biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
divalent metal cations are not required for enzyme catalysis
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
22
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
-
the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria
physiological function
-
the enzyme is involved in the O-repeating unit biosynthesis, the first committed step in lipopolysaccharide biosynthesis in a variety of gram-negative bacteria
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
to 2.2 A resolution, and modeling of both the donor substrate UDP-GalNAC and the acceptor substrate analog 1-L-Ins-1-P into the structure. UDP-GalNAC primarily binds to the C-lobe (i.e. residues 167322) and is surrounded by four conserved residues (i.e., His109, Lys192, Glu256 and Glu264). The acceptor analog 1-L-Ins-1-P binds to the N-lobe, and the tail portion extends into the solvent region in the model
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yi, W.; Yao, Q.; Zhang, Y.; Motari, E.; Lin, S.; Wang, P.G.
The wbnH gene of Escherichia coli O86:H2 encodes an alpha-1,3-N-acetylgalactosaminyl transferase involved in the O-repeating unit biosynthesis
Biochem. Biophys. Res. Commun.
344
631-639
2006
Escherichia coli, Escherichia coli 86:H2
brenda
Woodward, R.; Yi, W.; Li, L.; Zhao, G.; Eguchi, H.; Sridhar, P.R.; Guo, H.; Song, J.K.; Motari, E.; Cai, L.; Kelleher, P.; Liu, X.; Han, W.; Zhang, W.; Ding, Y.; Li, M.; Wang, P.G.
In vitro bacterial polysaccharide biosynthesis: defining the functions of Wzy and Wzz
Nat. Chem. Biol.
6
418-423
2010
Escherichia coli (Q5J7D6), Escherichia coli O86:B7 (Q5J7D6)
brenda
Li, F.; Li, S.; Liu, X.; Yang, X.; Wang, P.; Shen, Y.
The structure of WbnH in a near active state
Protein Cell
6
615-618
2015
Escherichia coli, Escherichia coli O86:H2
brenda
Falconer, D.; Phillippopoulos, K.; Czuchry, D.; Kocev, A.; Brockhausen, I.
Biosynthesis of Salmonella O43 and Escherichia coli O86 antigens Comparison of alpha1,3-GalNAc-transferases WfbG and WbnH
Carbohydr. Res.
552
109434
2025
Escherichia coli (P0DMP6), Escherichia coli O86 (P0DMP6)
brenda
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