We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Involved in the biosynthesis of kanamycin B and kanamycin C. Also catalyses EC 2.4.1.283, 2-deoxystreptamine N-acetyl-D-glucosaminyltransferase, but activity is only one fifth of that with UDP-alpha-D-glucose.
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kanF
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + 2-deoxystreptamine = UDP + 2'-deamino-2'-hydroxyparomamine
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose:2-deoxystreptamine 6-alpha-D-glucosyltransferase
Involved in the biosynthesis of kanamycin B and kanamycin C. Also catalyses EC 2.4.1.283, 2-deoxystreptamine N-acetyl-D-glucosaminyltransferase, but activity is only one fifth of that with UDP-alpha-D-glucose.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dTDP-D-glucose + 2-deoxystreptamine
?
GDP-D-mannose + 2-deoxystreptamine
?
UDP-D-galactose + 2-deoxystreptamine
?
UDP-D-glucose + 2-deoxystreptamine
UDP + 2'-deamino-2'-hydroxyparomamine
-
best substrate
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine
UDP + 2'-N-acetylparomamine
-
reaction of 2.4.1.283, one fifth of the catalytic efficiency with UDP-D-glucose
-
-
?
additional information
?
-
dTDP-D-glucose + 2-deoxystreptamine
?
-
100% activity
-
-
?
dTDP-D-glucose + 2-deoxystreptamine
?
-
100% activity
-
-
?
GDP-D-mannose + 2-deoxystreptamine
?
-
highest activity (about 200% compared to dTDP-D-glucose)
-
-
?
GDP-D-mannose + 2-deoxystreptamine
?
-
highest activity (about 200% compared to dTDP-D-glucose)
-
-
?
UDP-D-galactose + 2-deoxystreptamine
?
-
about 50% activity compared to dTDP-D-glucose
-
-
?
UDP-D-galactose + 2-deoxystreptamine
?
-
about 50% activity compared to dTDP-D-glucose
-
-
?
additional information
?
-
-
wild type KanF shows weak glucosylation activity for 2-deoxystreptamine and low activity towards UDP-D-glucose, dTDP-L-rhamnose, dTDP-4-keto-6-Ddeoxy-glucose, dTDP-D-fucosamine, and dTDP-2-deoxy-D-glucose
-
-
?
additional information
?
-
-
wild type KanF shows weak glucosylation activity for 2-deoxystreptamine and low activity towards UDP-D-glucose, dTDP-L-rhamnose, dTDP-4-keto-6-Ddeoxy-glucose, dTDP-D-fucosamine, and dTDP-2-deoxy-D-glucose
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.28 - 0.32
2-deoxystreptamine
0.44
UDP-D-glucose
-
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl22, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
2.54
UDP-N-acetyl-alpha-D-glucosamine
-
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
0.28
2-deoxystreptamine
-
using UDP-D-glucose as donor, in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
0.32
2-deoxystreptamine
-
using UDP-N-acetyl-alpha-D-glucosamine as donor, in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.29
UDP-D-glucose
-
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
0.33
UDP-N-acetyl-alpha-D-glucosamine
-
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.66
UDP-D-glucose
-
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
0.13
UDP-N-acetyl-alpha-D-glucosamine
-
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21(DE3) cells
-
KanF cannot be functionally expressed in Escherichia coli
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Park, S.H.; Park, H.Y.; Sohng, J.K.; Lee, H.C.; Liou, K.; Yoon, Y.J.; Kim, B.G.
Expanding substrate specificity of GT-B fold glycosyltransferase via domain swapping and high-throughput screening
Biotechnol. Bioeng.
102
988-994
2009
Streptomyces kanamyceticus, Streptomyces kanamyceticus ATCC 12853
brenda
Zhao, X.; Kim, K.; Sang, L.; Kang, S.; Yang, Y.; Suh, J.
Genetic organization of a 50-kb gene cluster isolated from Streptomyces kanamyceticus for kanamycin biosynthesis and characterization of kanamycin acetyltransferase
J. Microbiol. Biotechnol.
15
346-353
2005
Streptomyces kanamyceticus, Streptomyces kanamyceticus ATCC 12853
-
brenda
Park, J.; Park, S.; Nepal, K.; Han, A.; Ban, Y.; Yoo, Y.; Kim, E.; Kim, E.; Kim, D.; Sohng, J.; Yoon, Y.
Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation
Nat. Chem. Biol.
7
843-852
2011
Streptomyces kanamyceticus
brenda
Select items on the left to see more content.
html completed
BRENDA home
History
Enzyme Nomenclature
2.4.1.284 2-deoxystreptamine glucosyltransferase
top
