Any feedback?
No. of entries
Information on EC 2.4.1.252 - GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase
for references in articles please use BRENDA:EC2.4.1.252
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.252 GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase
IUBMB Comments
In the bacterium Gluconacetobacter xylinus (previously known as Acetobacter xylinum) the enzyme is involved in the biosynthesis of the exopolysaccharide acetan . In Xanthomonas campestris the enzyme is involved in the biosynthesis of the exopolysaccharide xanthan .
The enzyme appears in viruses and cellular organisms
Reaction Schemes
showSynonyms
gdp-mannosyltransferase, gdp-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase, alpha-mannosyltransferase acea, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AceA
gumH
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol = GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
MetaCyc
acetan biosynthesis, xanthan biosynthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
GDP-mannose:D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol 3-alpha-mannosyltransferase
In the bacterium Gluconacetobacter xylinus (previously known as Acetobacter xylinum) the enzyme is involved in the biosynthesis of the exopolysaccharide acetan [1]. In Xanthomonas campestris the enzyme is involved in the biosynthesis of the exopolysaccharide xanthan [5].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GDP-mannose + cellobiose-diphosphate-polyprenol carrier lipid
GDP + D-mannose-alpha-(1-3)-cellobiose-diphosphate-polyprenol carrier lipid
-
Substrates: GumH enzyme is an alpha-1,3-mannosyltransferase responsible for the transfer of mannose from GDP-mannose to the cellobiose-diphosphate-polyprenol carrier lipid during the assembly of the tetrasaccharide repeat unit of the exopolysaccharide fastidian gum
Products: -
Products: -
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphophytanol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphophytanol
-
Substrates: synthetic phytanyl-pyrophosphate-linked cellobiose functions as an acceptor for the AceA mannosyltransferase reaction in vitro. Neither the chain length nor the saturation state, notably of the first isoprene unit, are critical to the AceA mannosyltransferase activity
Products: -
Products: -
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
Substrates: the enzyme is involved in the biosynthesis of the exopolysaccharide acetan
Products: -
Products: -
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
Substrates: -
Products: -
Products: -
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
Substrates: AceA is not able to use free cellobiose as a substrate, indicating that the pyrophosphate-lipid moiety is needed for enzymatic activity
Products: characterisation of the in vitro reaction product
Products: characterisation of the in vitro reaction product
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
Substrates: -
Products: -
Products: -
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
Substrates: the enzyme is involved in the biosynthesis of the exopolysaccharide acetan
Products: -
Products: -
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
Substrates: EDTA-treated Xanthomonas campestris cells are used as both enzyme preparation and lipid-P acceptor, and UDP-Glc, GDP-Man, and UDP-glucuronic acid are used as sugar donors. A linear pentasaccharide unit is assembled on a polyprenol-phsophate lipid carrier by the sequential addition of glucose-1-phosphate, glucose, mannose, glucuronic acid, and mannose
Products: -
Products: -
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
-
Substrates: the enzyme is involved in biosynthesis of xanthan
Products: -
Products: -
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: AceA Lys211 and Glu295 are involved in nucleotide sugar donor binding. Glu287 of the EX7E is a potential catalytic residue
Products: -
Products: -
?
additional information
additional information
-
Substrates: GumH is involved in biosynthesis of the pentasaccharide repeating unit of xanthan. It is suggested that the wild-type Xanthomonas oryzae-produced xanthan is assembled by the sequential addition of UDP-glucose, UDP-glucose, GDP-mannose, UDPglucuronic acid, and GDP-mannose onto a polyprenol phosphate carrier, by the glycosyltransferase homologues encoded by the gumD, gumM, gumH, gumK, and gumI genes, respectively
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphosphopolyprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyprenol
-
Substrates: the enzyme is involved in biosynthesis of xanthan
Products: -
Products: -
?
additional information
additional information
-
Substrates: GumH is involved in biosynthesis of the pentasaccharide repeating unit of xanthan. It is suggested that the wild-type Xanthomonas oryzae-produced xanthan is assembled by the sequential addition of UDP-glucose, UDP-glucose, GDP-mannose, UDPglucuronic acid, and GDP-mannose onto a polyprenol phosphate carrier, by the glycosyltransferase homologues encoded by the gumD, gumM, gumH, gumK, and gumI genes, respectively
Products: -
Products: -
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
Substrates: the enzyme is involved in the biosynthesis of the exopolysaccharide acetan
Products: -
Products: -
?
GDP-mannose + D-Glc-beta-(1->4)-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
GDP + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol
-
Substrates: -
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Tuberculosis
Expression and purification of a functionally active recombinant GDP-mannosyltransferase (PimA) from Mycobacterium tuberculosis H37Rv.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
significant degree of homology with the alpha-mannosyltransferase GumH of Xanthomonas campestris
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
additional information
the gum gene cluster constitutes an operon expressed from multiple promoters located upstream of gumB and gumG, respectively. The existence of two internal promoters is newly identified in the Xanthomonas oryzae gum gene cluster through the insertion mutations with the rrnB transcriptional terminator and RT-PCR analyses
malfunction
-
from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumH completely abolishes in vitro polymer formation. Permeabilized cells are incubated with UDP-glucose, GDPmannose, and UDP-glucuronic acid, one of them labeled in the sugar moiety. Accumulating intermediates are analysed. Oligosaccharides obtained from gumH- strain XcH can be resolved into two components: a compound with the same mobility as the disaccharide cellobiose and glucose. XcH cells labeled with UDP-[14C]glucuronic acid or GDP-[14C]mannose do not incorporate radioactivity into the 1203 extract
malfunction
significantly attenuated production of xanthan in knock-out mutant strain
physiological function
-
the enzyme is involved in the biosynthesis of the exopolysaccharide acetan
physiological function
the enzyme is involved in acetan polysaccharide synthesis. AceA can complement a Xanthomonas campestris mutant that is deficient in the homologous gene gumH (involved in xanthan biosynthesis)
physiological function
-
the enzyme is involved in biosynthesis of xanthan
physiological function
GumH is involved in biosynthesis of the pentasaccharide repeating unit of xanthan
physiological function
-
GumH enzyme is an alpha-1,3-mannosyltransferase responsible for the transfer of mannose from GDP-mannose to the cellobiose-diphosphate-polyprenol carrier lipid during the assembly of the tetrasaccharide repeat unit of the exopolysaccharide fastidian gum
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GUMH_XANOP
Xanthomonas oryzae pv. oryzae (strain PXO99A)
380
0
42237
Swiss-Prot
-
Q5GXZ4_XANOR
Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)
380
0
42237
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
E295A
-
mutant enzyme retains residual activity in the in vivo assay
H127A
-
retains reduced but significant activity both in vitro and in vivo
S162A
-
retains reduced but significant activity both in vitro and in vivo
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
maltose-binding-protein-fused enzyme is partially purified by amylose column chromatography, His-tagged enzyme is purified by Ni-NTA column chromatography
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in fusion with maltose-binding protein in Escherichia coli BL21(DE3) cells and as His-tagged protein in Rosetta(DE3)pLysS cells
-
overproduced in Escherichia coli. The aceA ORF is subcloned into the expression vector pET29 in frame with the S-tag epitope. The recombinant protein is identified, and culture conditions are optimised for production of the soluble protein
-
recombinant mutant AceA expressed in Escherichia coli and in Xanthomonas campestris gumH- strain
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
-
method for real-time detection of recombinant GumH enzymatic activity using a Quartz Crystal Microbalance with dissipation monitoring, QCM-D. Monitoring the real-time cellobiose-diphosphate-polyprenol carrier lipid-QCM-D transducer in the presence of GDP-man and GumH enzyme shows a mass increase, indicating the transfer of mannose. The determination of mannosyltransferase function is validated by a HPLC method developed for determination of GDP produced by enzymatic reaction
synthesis
-
the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lellouch, A.C.; Watt, G.M.; Geremia, R.A.; Flitsch, S.L.
Phytanyl-pyrophosphate-linked substrate for a bacterial alpha-mannosyltransferase
Biochem. Biophys. Res. Commun.
272
290-292
2000
Komagataeibacter xylinus
brenda
Lee, C.K.; Lee, B.M.; Cho, J.Y.
Identification of new internal promoters of the Xanthomonas oryzae pathovar oryzae gum gene cluster
Biotechnol. Lett.
30
521-527
2008
Xanthomonas oryzae (Q5GXZ4)
brenda
Kim, S.Y.; Kim, J.G.; Lee, B.M.; Cho, J.Y.
Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae pv oryzae
Biotechnol. Lett.
31
265-270
2008
Xanthomonas oryzae (Q5GXZ4)
brenda
Ielpi, L.; Couso, R.O.; Dankert, M.A.
Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris
J. Bacteriol.
175
2490-2500
1993
Xanthomonas campestris
brenda
Petroni, E.A.; Ielpi, L.
Isolation and nucleotide sequence of the GDP-mannose:cellobiosyl-diphosphopolyprenol alpha-mannosyltransferase gene from Acetobacter xylinum
J. Bacteriol.
178
4814-4821
1996
Komagataeibacter xylinus (Q44571), Komagataeibacter xylinus
brenda
Katzen, F.; Ferreiro, D.U.; Oddo, C.G.; Ielmini, M.V.; Becker, A.; Phler, A.; Ielpi, L.
Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence
J. Bacteriol.
180
1607-1617
1998
Xanthomonas campestris
brenda
Abdian, P.L.; Lellouch, A.C.; Gautier, C.; Ielpi, L.; Geremia, R.A.
Identification of essential amino acids in the bacterial
J. Biol. Chem.
275
40568-40575
2000
Komagataeibacter xylinus
brenda
Geremia, R.A.; Roux, M.; Ferreiro, D.U.; Dauphin-Dubois, R.; Lellouch, A.C.; Ielpi, L.
Expression and biochemical characterisation of recombinant AceA, a bacterial alpha-mannosyltransferase
Mol. Gen. Genet.
261
933-940
1990
Komagataeibacter xylinus
brenda
Muniz, J.R.; Alves, C.A.; de Pieri, C.; Beltramini, L.M.; Selistre-de-Araujo, H.S.; Vettore, A.L.; da Silva, F.R.; Arruda, P.; Garratt, R.C.; Oliva, G.; Souza, D.H.
Overexpression, purification, biochemical characterization, and molecular modeling of recombinant GDP-mannosyltransferase (GumH) from Xylella fastidiosa
Biochem. Biophys. Res. Commun.
315
485-492
2004
Xylella fastidiosa
brenda
Alves, C.A.; Pedroso, M.M.; de Moraes, M.C.; Souza, D.H.; Cass, Q.B.; Faria, R.C.
Real-time investigation of mannosyltransferase function of a Xylella fastidiosa recombinant GumH protein using QCM-D
Biochem. Biophys. Res. Commun.
408
571-575
2011
Xylella fastidiosa
brenda
EXTERNAL LINKS (specific for EC-Number 2.4.1.252)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
