Information on EC 2.4.1.251 - GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-ditrans,octacis-undecaprenol 4-beta-mannosyltransferase
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Substrates: EDTA-treated Xanthomonas campestris cells are used as both enzyme preparation and lipid-P acceptor, and UDP-Glc, GDP-Man, and UDP-glucuronic acid are used as sugar donors. A linear pentasaccharide unit is assembled on a polyprenol-P lipid carrier by the sequential addition of glucose-1-P, glucose, mannose, glucuronic acid, and mannose Products: -
Substrates: enzyme is a nonprocessive beta-mannosyltransferase catalyzing the transfer of a mannose residue from GDP-Man to glucuronic acid-beta-1,2-mannose-alpha-1,3-glucose-beta-1,4-glucose-PP-polyisoprenyl to form the lipid-linked pentasaccharide Products: -
Substrates: GumK is involved in biosynthesis of the pentasaccharide repeating unit of xanthan. It is suggested that the wild-type Xanthomonas oryzae-produced xanthan is assembled by the sequential addition of UDP-glucose, UDP-glucose, GDP-mannose, UDP-glucuronic acid, and GDP-mannose onto a polyprenol phosphate carrier, by the glycosyltransferase homologues encoded by the gumD, gumM, gumH, gumK, and gumI genes, respectively Products: -
Substrates: GumK is involved in biosynthesis of the pentasaccharide repeating unit of xanthan. It is suggested that the wild-type Xanthomonas oryzae-produced xanthan is assembled by the sequential addition of UDP-glucose, UDP-glucose, GDP-mannose, UDP-glucuronic acid, and GDP-mannose onto a polyprenol phosphate carrier, by the glycosyltransferase homologues encoded by the gumD, gumM, gumH, gumK, and gumI genes, respectively Products: -
from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumK completely abolishes in vitro polymer formation. Permeabilized cells are incubated with UDP-glucose, GDPmannose, and UDP-glucuronic acid, one of them labeled in the sugar moiety. Accumulating intermediates are analysed. Accumulation of GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha1-diphospho-(lipid carrier) in the gumI deletion strain. Mutations in gumI have less effect on the amount of in vitro produced polysaccharide. The gumI deletion strain produces about 10% of the polymer amount produced by the wild-type strain
decreased amount of the exopolysaccharide produced by the gumI mutant strain SJ1021 may be due to incomplete polymerization of the tetrasaccharide repeating units. Although this strain is not fully capable of producing the pentasaccharide repeating units, the absence of a terminal mannose residue in xanthan does not affect the full virulence of Xanthomonas oryzae
Salinas, S.R.; Bianco, M.I.; Barreras, M.; Ielpi, L.
Expression, purification and biochemical characterization of GumI, a monotopic membrane GDP-mannose:glycolipid 4-beta-D-mannosyltransferase from Xanthomonas campestris pv. campestris
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