We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Isolated from the plant Gentiana triflora (clustered gentian).
The expected taxonomic range for this enzyme is: Gentiana triflora
Synonyms
3'GT, anthocyanin 3'-O-beta-D-glucosyltransferase, UDP-glucose:anthocyanin 3'-O-glucosyltransferase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
anthocyanin 3'-O-beta-D-glucosyltransferase
-
-
-
-
UDP-glucose:anthocyanin 3'-O-glucosyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + delphinidin 3,5-di-O-beta-D-glucoside = UDP + delphinidin 3,3',5-tri-O-beta-D-glucoside
UDP-alpha-D-glucose + delphinidin 3,5-di-O-beta-D-glucoside = UDP + delphinidin 3,3',5-tri-O-beta-D-glucoside
this enzyme specifically glucosylates the 3'-hydroxy group of delphinidin-3-O-glucosyl-5-O-(6-O-caffeoylglucosyl)-3'-O-(6-O-caffeoylglucoside)-type anthocyanins containing glucose groups at the 3 and 5 positions, acts on delphinidin 3,5-di-O-glucoside in gentian, Gentiana triflora
UDP-alpha-D-glucose + delphinidin 3,5-di-O-beta-D-glucoside = UDP + delphinidin 3,3',5-tri-O-beta-D-glucoside
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
transfer of glycosyl group
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose:delphinidin-3,5-di-O-beta-D-glucoside 3'-O-glucosyltransferase
Isolated from the plant Gentiana triflora (clustered gentian).
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-glucose + delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside)
UDP + delphinidin 3-O,3'-O-di-beta-D-glucosyl-5-O-(6-O-caffeoyl-beta-D-glucoside)
in vitro, 50.9% of the activity with delphinidin 3,5-diglucoside
-
-
?
UDP-glucose + an anthocyanidin
UDP + an anthocyanidin 3'-O-beta-D-glucoside
strict substrate specificity, 3'GT specifically glucosylates the 3'-hydroxy group of delphinidin-type anthocyanins containing glucose groups at the 3 and 5 positions and specifically uses UDP-glucose as the sugar donor, 3âGT strictly requires three hydroxy groups on the B-ring for its substrates
-
-
?
UDP-glucose + delphinidin
UDP + delphinidin 3'-glucoside
poor substrate, 3.3% of the activity with delphinidin 3,5-diglucoside
-
-
?
UDP-glucose + delphinidin 3,5-di-O-beta-D-glucoside
UDP + delphinidin 3,5,7-tri-O-beta-D-glucoside
3'GT glucosylates the 3'-hydroxy group of delphinidin 3,5-diglucoside, 2fold higher activity than with delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside) as substrate
-
-
?
UDP-glucose + delphinidin 3-glucoside
UDP + delphinidin 3,3'-diglucoside
poor substrate, 2.9% of the activity with delphinidin 3,5-diglucoside
-
-
?
additional information
?
-
additional information
?
-
key enzyme for blue anthocyanin biosynthesis, biosynthesis of gentiodelphin
-
-
?
additional information
?
-
no sugar donors: UDP-galactose, UDP-GlcUA, UDP-N-acetylgalactosamine, not: pelargonidin 3,5-diglucoside, cyanidin 3,5-diglucoside, dihydroflavonols, dihydrokaempferol, dihydroquercetin, dihydromyricetin, flavone, tricetin
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
?
-
key enzyme for blue anthocyanin biosynthesis, biosynthesis of gentiodelphin
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP
competitive inhibition
additional information
not inhibited by Mg2+, Ca2+, Mn2+, EDTA or EGTA
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.12
delphinidin 3,5-diglucoside
pH 8.5, 30°C, recombinant 3'GT
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.76
UDP
pH 8.5, 30°C, recombinant 3'GT
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
var. japonica
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
blue, from flowers at a pre-anthesis stage, 3'GT mRNA is abundant in petals at the first three developmental stages, but dramatically decreased in fully opened flowers
brenda
additional information
3'GT mRNA is barely detectable in leaves
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
recombinant 3'GT expressed in Escherichia coli JM105
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ANGT_GENTR
482
0
54041
Swiss-Prot
other Location (Reliability: 5 )
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
54037
x * 55000, SDS-PAGE, x * 54037, sequence calculation
55000
x * 55000, SDS-PAGE, x * 54037, sequence calculation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
x * 55000, SDS-PAGE, x * 54037, sequence calculation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
native 3'GT from petals, recombinant 3'GT expressed in Escherichia coli JM105
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in transgenic Petunia hybrida and in Escherichia coli JM105, sequencing
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
agriculture
3'GT cDNA is a useful molecular tool for modification of flower color using genetic engineering, production of blue anthocyanins
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Fukuchi-Mizutani, M.; Okuhara, H.; Fukui, Y.; Nakao, M.; Katsumoto, Y.; Yonekura-Sakakibara, K.; Kusumi, T.; Hase, T.; Tanaka, Y.
Biochemical and molecular characterization of a novel UDP-glucose:anthocyanin 3'-O-glucosyltransferase, a key enzyme for blue anthocyanin biosynthesis, from gentian
Plant Physiol.
132
1652-1663
2003
Gentiana triflora (Q8H0F2)
brenda
Select items on the left to see more content.
html completed