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Information on EC 2.4.1.238 - delphinidin 3,5-di-O-glucoside 3'-O-glucosyltransferase for references in articles please use BRENDA:EC2.4.1.238
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EC Tree
IUBMB Comments Isolated from the plant Gentiana triflora (clustered gentian).
The expected taxonomic range for this enzyme is: Gentiana triflora
Synonyms
3'GT, anthocyanin 3'-O-beta-D-glucosyltransferase, UDP-glucose:anthocyanin 3'-O-glucosyltransferase,
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anthocyanin 3'-O-beta-D-glucosyltransferase
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UDP-glucose:anthocyanin 3'-O-glucosyltransferase
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UDP-alpha-D-glucose + delphinidin 3,5-di-O-beta-D-glucoside = UDP + delphinidin 3,3',5-tri-O-beta-D-glucoside
UDP-alpha-D-glucose + delphinidin 3,5-di-O-beta-D-glucoside = UDP + delphinidin 3,3',5-tri-O-beta-D-glucoside
this enzyme specifically glucosylates the 3'-hydroxy group of delphinidin-3-O-glucosyl-5-O-(6-O-caffeoylglucosyl)-3'-O-(6-O-caffeoylglucoside)-type anthocyanins containing glucose groups at the 3 and 5 positions, acts on delphinidin 3,5-di-O-glucoside in gentian, Gentiana triflora
UDP-alpha-D-glucose + delphinidin 3,5-di-O-beta-D-glucoside = UDP + delphinidin 3,3',5-tri-O-beta-D-glucoside
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transfer of glycosyl group
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UDP-alpha-D-glucose:delphinidin-3,5-di-O-beta-D-glucoside 3'-O-glucosyltransferase
Isolated from the plant Gentiana triflora (clustered gentian).
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UDP-alpha-D-glucose + delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside)
UDP + delphinidin 3-O,3'-O-di-beta-D-glucosyl-5-O-(6-O-caffeoyl-beta-D-glucoside)
in vitro, 50.9% of the activity with delphinidin 3,5-diglucoside
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UDP-glucose + an anthocyanidin
UDP + an anthocyanidin 3'-O-beta-D-glucoside
strict substrate specificity, 3'GT specifically glucosylates the 3'-hydroxy group of delphinidin-type anthocyanins containing glucose groups at the 3 and 5 positions and specifically uses UDP-glucose as the sugar donor, 3’GT strictly requires three hydroxy groups on the B-ring for its substrates
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UDP-glucose + delphinidin
UDP + delphinidin 3'-glucoside
poor substrate, 3.3% of the activity with delphinidin 3,5-diglucoside
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UDP-glucose + delphinidin 3,5-di-O-beta-D-glucoside
UDP + delphinidin 3,5,7-tri-O-beta-D-glucoside
3'GT glucosylates the 3'-hydroxy group of delphinidin 3,5-diglucoside, 2fold higher activity than with delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside) as substrate
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UDP-glucose + delphinidin 3-glucoside
UDP + delphinidin 3,3'-diglucoside
poor substrate, 2.9% of the activity with delphinidin 3,5-diglucoside
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additional information
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additional information
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key enzyme for blue anthocyanin biosynthesis, biosynthesis of gentiodelphin
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additional information
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no sugar donors: UDP-galactose, UDP-GlcUA, UDP-N-acetylgalactosamine, not: pelargonidin 3,5-diglucoside, cyanidin 3,5-diglucoside, dihydroflavonols, dihydrokaempferol, dihydroquercetin, dihydromyricetin, flavone, tricetin
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additional information
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Q8H0F2
key enzyme for blue anthocyanin biosynthesis, biosynthesis of gentiodelphin
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UDP
competitive inhibition
additional information
not inhibited by Mg2+, Ca2+, Mn2+, EDTA or EGTA
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0.12
delphinidin 3,5-diglucoside
pH 8.5, 30°C, recombinant 3'GT
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0.76
UDP
pH 8.5, 30°C, recombinant 3'GT
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var. japonica
SwissProt
brenda
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blue, from flowers at a pre-anthesis stage, 3'GT mRNA is abundant in petals at the first three developmental stages, but dramatically decreased in fully opened flowers
brenda
additional information
3'GT mRNA is barely detectable in leaves
brenda
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recombinant 3'GT expressed in Escherichia coli JM105
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brenda
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ANGT_GENTR
482
0
54041
Swiss-Prot
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54037
x * 55000, SDS-PAGE, x * 54037, sequence calculation
55000
x * 55000, SDS-PAGE, x * 54037, sequence calculation
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x * 55000, SDS-PAGE, x * 54037, sequence calculation
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native 3'GT from petals, recombinant 3'GT expressed in Escherichia coli JM105
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expression in transgenic Petunia hybrida and in Escherichia coli JM105, sequencing
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agriculture
3'GT cDNA is a useful molecular tool for modification of flower color using genetic engineering, production of blue anthocyanins
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Fukuchi-Mizutani, M.; Okuhara, H.; Fukui, Y.; Nakao, M.; Katsumoto, Y.; Yonekura-Sakakibara, K.; Kusumi, T.; Hase, T.; Tanaka, Y.
Biochemical and molecular characterization of a novel UDP-glucose:anthocyanin 3'-O-glucosyltransferase, a key enzyme for blue anthocyanin biosynthesis, from gentian
Plant Physiol.
132
1652-1663
2003
Gentiana triflora (Q8H0F2)
brenda
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2.4.1.238 delphinidin 3,5-di-O-glucoside 3'-O-glucosyltransferase
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