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Information on EC 2.4.1.220 - indoxyl-UDPG glucosyltransferase
for references in articles please use BRENDA:EC2.4.1.220
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.220 indoxyl-UDPG glucosyltransferase
IUBMB Comments
Also acts to a limited extent on 4-, 5-, 6- and 7-hydroxyindole. After enzymic or chemical hydrolysis, indican forms indoxyl, which, in turn, is converted in the presence of oxygen to the dye indigo.
The enzyme appears in viruses and cellular organisms
showSynonyms
ptigs, indican synthase, ptugt1, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
hydroxyindole glucosyltransferase
-
-
-
-
indican synthase
-
-
-
-
indoxyl glucosyltransferase
-
-
-
-
indoxyl-UDPG-glucolsyltransferase
-
-
-
-
PtUGT2
-
differs from PtUGT1 by four amino acid residues V19M, G225A, E230Q, and A423D
UDP-glucose:indoxyl glucosyltransferase
UDP-glucose:indoxyl glucosyltransferase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + indoxyl = UDP + indican
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycosyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
indican biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:indoxyl 3-O-beta-D-glucosyltransferase
Also acts to a limited extent on 4-, 5-, 6- and 7-hydroxyindole. After enzymic or chemical hydrolysis, indican forms indoxyl, which, in turn, is converted in the presence of oxygen to the dye indigo.
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CAS REGISTRY NUMBER
COMMENTARY
258339-72-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 4-hydroxyindole
UDP + 1H-indol-4-yl-beta-D-glucopyranoside
-
Substrates: minimal activity
Products: -
Products: -
?
UDP-glucose + 5-hydroxyindole
UDP + 1H-indol-5-yl-beta-D-glucopyranoside
-
Substrates: activity as 3-OH-indole
Products: -
Products: -
?
UDP-glucose + 6-hydroxyindole
UDP + 1H-indol-6-yl-beta-D-glucopyranoside
-
Substrates: minimal activity
Products: -
Products: -
?
UDP-glucose + 7-hydroxyindole
UDP + 1H-indol-7-yl-beta-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
-
Substrates: best substrate
Products: i.e. indican
Products: i.e. indican
?
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
-
Substrates: best substrate
Products: i.e. indican
Products: i.e. indican
?
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
-
Substrates: i.e. indole-3-ol or 3-OH-indole
Products: i.e. indican
Products: i.e. indican
?
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
-
Substrates: indole is the biosynthetic precursor to the indoxyl derivates indican isatan B
Products: i.e. indican
Products: i.e. indican
?
UDP-glucose + indoxyl
UDP + indican
Substrates: the enzyme functions as an indican synthase in plant cells
Products: -
Products: -
?
UDP-glucose + indoxyl
UDP + indican
Substrates: -
Products: -
Products: -
?
UDP-glucose + indoxyl
UDP + indican
Substrates: -
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + indoxyl
UDP + 1H-indole-3-yl-beta-D-glucopyranoside
-
Substrates: indole is the biosynthetic precursor to the indoxyl derivates indican isatan B
Products: i.e. indican
Products: i.e. indican
?
UDP-glucose + indoxyl
UDP + indican
Substrates: the enzyme functions as an indican synthase in plant cells
Products: -
Products: -
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
in expression analyses PtIGS mRNA is found only in the leaves. It is most highly expressed in the 1st leaves, and decreased with leaf age
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
microsomal PtIGS is soluble in the presence of detergents and urea and is strongly associated with membranes. The enzyme interacts with some kind of proteins on ER membranes to certainly carry out a delivery of substrate
brenda
microsomal PtIGS is soluble in the presence of detergents and urea and is strongly associated with membranes
-
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
the enzyme (PtUGT1) is cocrystallized with indoxyl sulfate in sitting drops consisting of 0.0002 ml protein solution and 0.0002 ml crystallization buffer. Crystal structure is solved at 2.14 A resolution with indoxyl sulfate, a glycosylation-incompetent indoxyl analog, in the active site
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
E75P/Q86K/S110V/I188L/G222D/G296L/V297G/T388A/G430K/S413K
-
mutant with increased Tm value, robust in high substrate concentration, heat, organic solvents, prolonged storage, and unbuffered water. Mutant synthesizes indican in vitro with a total turnover number > 250000 in 24 h
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
63.1
-
melting temperature, mutant E75P/Q86K/S110V/I188L/G222D/G296L/V297G/T388A/G430K/S413K
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
anaerobic conditions, since under aerobic conditions indoxyl dimerizes spontaneously to indigo
-
488949
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
industry
-
rational engineering of UGT1 to allow a mild, light-driven process in denim dyeing. Mutant E75P/Q86K/S110V/I188L/G222D/G296L/V297G/T388A/G430K/S413K supports an economically viable production of indican in the existing indigo production pipelines
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Marcinek, H.; Weyler, W.; Deus-Neumann, B.; Zenk, M.H.
Indoxyl-UDPG-glucosyltransferase from Baphicacanthus cusia
Phytochemistry
53
201-207
2000
Strobilanthes cusia
brenda
Minami, Y.; Nishimura, O.; Hara-Nishimura, I.; Nishimura, M.; Matsubara, H.
Tissue and intracellular localization of indican and the purification and characterization of indican synthase from indigo plants
Plant Cell Physiol.
41
218-225
2000
Persicaria tinctoria
brenda
Hsu, T.M.; Welner, D.H.; Russ, Z.N.; Cervantes, B.; Prathuri, R.L.; Adams, P.D.; Dueber, J.E.
Employing a biochemical protecting group for a sustainable indigo dyeing strategy
Nat. Chem. Biol.
14
256-261
2018
Persicaria tinctoria, Persicaria tinctoria (A0A2R2JFJ4)
brenda
Inoue, S.; Morita, R.; Kuwata, K.; Kunieda, T.; Ueda, H.; Hara-Nishimura, I.; Minami, Y.
Tissue-specific and intracellular localization of indican synthase from Polygonum tinctorium
Plant Physiol. Biochem.
132
138-144
2018
Persicaria tinctoria (A0A2L2R220), Persicaria tinctoria
brenda
Bidart, G.N.; Teze, D.; Jansen, C.U.; Pasutto, E.; Putkaradze, N.; Sesay, A.M.; Fredslund, F.; Lo Leggio, L.; Oegmundarson, O.; Sukumara, S.; Qvortrup, K.; Welner, D.H.
Chemoenzymatic indican for light-driven denim dyeing
Nat. Commun.
15
1489
2024
Persicaria tinctoria
brenda
EXTERNAL LINKS (specific for EC-Number 2.4.1.220)
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