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Information on EC 2.4.1.208 - diglucosyl diacylglycerol synthase (1,2-linking)
for references in articles please use BRENDA:EC2.4.1.208
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.208 diglucosyl diacylglycerol synthase (1,2-linking)
IUBMB Comments
The enzyme from Acholeplasma laidlawii requires Mg2+.
The enzyme appears in viruses and cellular organisms
- 2.4.1.208
- polynucleotide
- rnase
- exoribonuclease
-
ribonuclease
-
helicase
-
exonuclease
-
degradosome
-
stem-loop
- acholeplasma
-
nonbilayer-prone
-
curvature
-
exonucleolytic
-
equilibria
- laidlawii
-
nonbilayer
-
intermembrane
-
yersiniae
-
mglcdag
- phosphatidylglycerol
Reaction Schemes
showSynonyms
dglcdag synthase, diglucosyldiacylglycerol synthase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1,2-diacylglycerol-3-alpha-glucosyl transferase
DGlcDAG synthase
diglucosyldiacylglycerol synthase
-
-
-
-
glucosyltransferase, uridine diphosphoglucose-monoglucosyldiacylglycerol
-
-
-
-
MgGlcDAG (1->2) glucosyltransferase
-
-
-
-
monoglucosyl diacylglycerol (1->2) glucosyltransferase
-
-
-
-
monoglucosyldiacylglycerol glucosyltransferase
-
-
-
-
DGlcDAG synthase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->2)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->2)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
-
-
-
-
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->2)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
the enzyme is made from 1,2-diacylglycerol-3-glucosyltransferase, EC 2.4.1.157, in membranes with more unsaturated or longer acyl chains, increased temperature or increased amount of cholesterol
-
UDP-alpha-D-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1->2)-O-alpha-D-glucopyranosyl]-sn-glycerol + UDP
the enzyme is made from 1,2-diacylglycerol-3-glucosyltransferase, EC 2.4.1.157, in membranes with more unsaturated or longer acyl chains, increased temperature or increased amount of cholesterol
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
alpha-diglucosyldiacylglycerol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol 2-glucosyltransferase
The enzyme from Acholeplasma laidlawii requires Mg2+.
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CAS REGISTRY NUMBER
COMMENTARY
168680-19-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1-2)-O-alpha-D-glucopyranosyl)sn-glycerol + UDP
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
UDP-glucose + 1,2-diheptadecanoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diheptadecanoyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: best substrate
Products: 73% of activity with monoglucosyldioleoylglycerol
Products: 73% of activity with monoglucosyldioleoylglycerol
?
UDP-glucose + 1,2-dioleoyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-dioleoyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1-2)-O-alpha-D-glucopyranosyl)sn-glycerol + UDP
-
Substrates: enzyme is involved in biosynthesis of phosphoglucolipids and has a regulatory function together with 1,2-diacylglycerol-3-glucosyltransferase, EC 2.4.1.157, overview
Products: -
Products: -
ir
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1-2)-O-alpha-D-glucopyranosyl)sn-glycerol + UDP
-
Substrates: -
Products: -
Products: -
ir
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1-2)-O-alpha-D-glucopyranosyl)sn-glycerol + UDP
-
Substrates: enzyme is involved in biosynthesis of phosphoglucolipids and has a regulatory function together with 1,2-diacylglycerol-3-glucosyltransferase, EC 2.4.1.157, overview
Products: -
Products: -
ir
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1-2)-O-alpha-D-glucopyranosyl)sn-glycerol + UDP
-
Substrates: -
Products: -
Products: -
ir
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: no activity if the sugar is in beta-position
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: at physiological concentrations of phosphatidylglycerol different nonlamellar-promoting pertubants can strongly enhance the synthesis of diglycosyldiacylglycerol in relation to their nonbilayer propensities
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: modulation of the activity by non-bilayer-forming lipids
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: enzyme provides glycolipid for lipoteichoic acid assembly
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: enzyme provides glycolipid for lipoteichoic acid assembly
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: bilayer binding by the enzyme, overview, the enzyme is made from 1,2-diacylglycerol-3-glucosyltransferase, EC 2.4.1.157, in membranes with more unsaturated or longer acyl chains, increased temperature or increased amount of cholesterol
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificty, the enzyme accepts pyrenyl lipid derivatives, resulting lipid organization, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: bilayer binding by the enzyme, overview, the enzyme is made from 1,2-diacylglycerol-3-glucosyltransferase, EC 2.4.1.157, in membranes with more unsaturated or longer acyl chains, increased temperature or increased amount of cholesterol
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate specificty, the enzyme accepts pyrenyl lipid derivatives, resulting lipid organization, overview
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1-2)-O-alpha-D-glucopyranosyl)sn-glycerol + UDP
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1-2)-O-alpha-D-glucopyranosyl)sn-glycerol + UDP
-
Substrates: enzyme is involved in biosynthesis of phosphoglucolipids and has a regulatory function together with 1,2-diacylglycerol-3-glucosyltransferase, EC 2.4.1.157, overview
Products: -
Products: -
ir
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1-2)-O-alpha-D-glucopyranosyl)sn-glycerol + UDP
-
Substrates: enzyme is involved in biosynthesis of phosphoglucolipids and has a regulatory function together with 1,2-diacylglycerol-3-glucosyltransferase, EC 2.4.1.157, overview
Products: -
Products: -
ir
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: at physiological concentrations of phosphatidylglycerol different nonlamellar-promoting pertubants can strongly enhance the synthesis of diglycosyldiacylglycerol in relation to their nonbilayer propensities
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: modulation of the activity by non-bilayer-forming lipids
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: enzyme provides glycolipid for lipoteichoic acid assembly
Products: -
Products: -
?
UDP-glucose + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol
UDP + 1,2-diacyl-3-O-(alpha-D-glucopyranosyl(1->2)-O-alpha-D-glucopyranosyl)-sn-glycerol
-
Substrates: enzyme provides glycolipid for lipoteichoic acid assembly
Products: -
Products: -
?
additional information
?
-
-
Substrates: bilayer binding by the enzyme, overview, the enzyme is made from 1,2-diacylglycerol-3-glucosyltransferase, EC 2.4.1.157, in membranes with more unsaturated or longer acyl chains, increased temperature or increased amount of cholesterol
Products: -
Products: -
?
additional information
?
-
-
Substrates: bilayer binding by the enzyme, overview, the enzyme is made from 1,2-diacylglycerol-3-glucosyltransferase, EC 2.4.1.157, in membranes with more unsaturated or longer acyl chains, increased temperature or increased amount of cholesterol
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mg2+
-
maximal activity at 10 mM, inhibition above may be due to interaction with UDP-glucose
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1,2-diacyl-3-O-[alpha-D-glucopyranosyl-1,2-O-(6-O-acyl-alpha-D-glucopyranosyl)]-sn-glycerol
-
-
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1,2-dioleoylphosphatidylserine
-
33% of activation with 1,2-dioleoylphosphatidylglycerol
diphosphatidylglycerol
-
from bovine, 50% of activation with 1,2-dioleoylphosphatidylglycerol
phosphatidylinositol
-
strong activation in the presence of 1,2-dioleoylphosphatidylglycerol
phosphatidylinositol-4,5-bisphosphate
-
strong activation in the presence of 1,2-dioleoylphosphatidylglycerol
phosphoenolpyruvate
-
0.5 mM, 4fold stimulation, inhibition above 1.5 mM
cardiolipin
-
very strong stimulation in the presence of 1,3-dioleoylglycerol
Lipid
-
activity is influenced in a dose-dependent manner by the nonbilayer propensities of several amphiphilic and hydrophilic lipids in two different bilayer matrices
phosphatidylglycerol
-
enzyme needs a polar activator lipid, preferably phosphatidylglycerol at concentrations of 25-30 mol% in zwitterionic mixed CHAPS bilayer-like aggregates
phosphatidylglycerol
-
in vivo amounts are not enough for efficient diglucosyldiacylglycerol synthesis
additional information
-
the enzyme is made from 1,2-diacylglycerol-3-glucosyltransferase, EC 2.4.1.157, in membranes with more unsaturated or longer acyl chains, increased temperature or increased amount of cholesterol
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity of the recombinant enzyme does not change during 3 months of storage and upon multiple cycles of freezing and thawing
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
enzyme can be reconstituted in liposome bilayers by the dialyse techique
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Karlsson, O.P.; Rytmaa, M.; Dahlqvist, A.; Kinnunen, P.K.L.; Wieslander, A.
Correlation between bilayer lipid dynamics and activity of the diglucosyldiacylglycerol synthase from Acholeplasma laidlawii membranes
Biochemistry
35
10094-10102
1996
Acholeplasma laidlawii
brenda
Dahlqvist, A.; Nordstrm, S.; Karlsson, O.P.; Mannock, D.A.; McElhaney, R.N.; Wieslander, A.
Efficient modulation of glucolipid enzyme activities in membranes of Acholeplasma laidlawii by the type of lipids in the bilayer matrix
Biochemistry
34
13381-13389
1995
Acholeplasma laidlawii
brenda
Cornell, R.B.; Arnold, R.S.
Modulation of the activities of enzymes of membrane lipid metabolism by non-bilayer-forming lipids
Chem. Phys. Lipids
81
215-227
1996
Acholeplasma laidlawii
-
brenda
Vikstrom, S.; Li, L.; Karlsson, O.P.; Wieslander, A.
Key role of the diglucosyldiacylglycerol synthase for the nonbilayer-bilayer lipid balance of Acholeplasma laidlawii membranes
Biochemistry
38
5511-5520
1999
Acholeplasma laidlawii
brenda
Vikstrom, S.; Li, L.; Wieslander, A.
The nonbilayer/bilayer lipid balance in membranes: Regulatory enzyme in Acholeplasma laidlawii is stimulated by metabolic phosphates, activator phospholipids, and double-stranded DNA
J. Biol. Chem.
275
9296-9302
2000
Acholeplasma laidlawii
brenda
Kiriukhin, M.Y.; Debabov, D.V.; Shinabarger, D.L.; Neuhaus, F.C.
Biosynthesis of the glycolipid anchor in lipoteichoic acid of Staphylococcus aureus RN4220: role of YpfP, the diglucosyldiacylglycerol synthase
J. Bacteriol.
183
3506-3514
2001
Bacillus subtilis, Staphylococcus aureus, Staphylococcus aureus RN4220
brenda
Edman, M.; Berg, S.; Storm, P.; Wikstrom, M.; Vikstrom, S.; Ohman, A.; Wieslander, A.
Structural features of glycosyltransferases synthesizing major bilayer and nonbilayer-prone membrane lipids in Acholeplasma laidlawii and Streptococcus pneumoniae
J. Biol. Chem.
278
8420-8428
2003
Acholeplasma laidlawii, Streptococcus pneumoniae
brenda
Storm, P.; Li, L.; Kinnunen, P.; Wieslander, A.
Lateral organization in Acholeplasma laidlawii lipid bilayer models containing endogenous pyrene probes
Eur. J. Biochem.
270
1699-1709
2003
Acholeplasma laidlawii, Acholeplasma laidlawii A-EF22
brenda
EXTERNAL LINKS (specific for EC-Number 2.4.1.208)
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