Information on EC 2.4.1.206 - lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase and Organism(s) Homo sapiens

for references in articles please use BRENDA:EC2.4.1.206
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The taxonomic range for the selected organisms is: Homo sapiens

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.4.1.206
-
RECOMMENDED NAME
GeneOntology No.
lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
lacto-series glycosphingolipids biosynthesis
-
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neolacto-series glycosphingolipids biosynthesis
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Glycosphingolipid biosynthesis - lacto and neolacto series
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide 3-beta-N-acetylglucosaminyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
83682-80-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1-4)-beta-D-glucosyl-(1-1)-ceramide
UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
-
-
-
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->4)Manbeta1->3[Galbeta1->4GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->6)Manbeta1->6]Manbeta1->4GlcNAc
?
show the reaction diagram
-
-
-
-
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
additional information
?
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beta3Gn-T2 associates with beta3Gn-T8 in vivo
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
show the reaction diagram
additional information
?
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beta3Gn-T2 associates with beta3Gn-T8 in vivo
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12-O-tetradecanoylphorbol-13-acetate
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down-regulation of enzyme expression
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta3Gn-T8
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beta3Gn-T2 is activated by beta3Gn-T8
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retinoic acid
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up-regulation of enzyme expression
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.42
Galbeta(1->4)GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->4)Manbeta1->3[Galbeta1->4GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->6)Manbeta1->6]Manbeta1->4GlcNAc
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betaGn3-T2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000034
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colonic mucosa
0.0000046
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colonic adenocarcinoma tissue sample
0.00121
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recombinant enzyme, substrate lactosylceramide
0.00123
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recombinant enzyme, substrate lD-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
0.00174
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recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
0.0021
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recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
0.00254
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recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
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assay at
7.3
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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not epithelial cells
Manually annotated by BRENDA team
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very low content
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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low content
Manually annotated by BRENDA team
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Daoy and D341 Med, cultured both in vitro and as xenografts in nude mice
Manually annotated by BRENDA team
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low content
Manually annotated by BRENDA team
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low content
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant FLAG-tagged fusion protein from Sf21 insect cells
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination, functional expression in Spodoptera frugiperda Sf9 cells via baculovirus infection
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DNA sequence determination and analysis; expression in Nawalma cells, membrane-bound; soluble expression as FLAG-tagged protein in Spodoptera frugiperda Sf21 cells via baculovirus infection
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expressed in COS-7 cells
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expression in Escherichia coli, overexpression of beta3GnT5 in MNK-45 cells and AGS cells, transfection with lipofectamine
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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adhesion of Helicobacter pylori to epithelial cells is mediated by bacterial adhesins that bind carbohydrate ligands expressed by the epithelial gastric cells, bea3GnT5 transferase induction is specific to infection with highly pathogenic strains