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Information on EC 2.4.1.206 - lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
for references in articles please use BRENDA:EC2.4.1.206
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.206 lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
The enzyme appears in viruses and cellular organisms
Reaction Schemes
showSynonyms
glcnac transferase, beta3gn-t5, beta3gn-t2, lc3 synthase, beta3gnt5, lactosylceramide n-acetylglucosaminyltransferase, beta1-3-n-acetylglucosaminyltransferase, la2 synthase, lc3 synthase/bgn-t5, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-lactosylceramide beta-
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-
-
-
beta1,3 N-acetylglucosaminyltransferase GlcNAc(beta1,3)Gal(beta1,4)Glc-ceramide synthase
beta1-3-N-acetylglucosaminyltransferase
-
-
-
-
beta3Gn-T5
LA2 synthase
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-
-
-
lactosylceramide beta-acetylglucosaminyltransferase
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-
-
-
lactosylceramide: N-acetylglucosaminyltransferase
lactotriaosylceramide synthase
Lc3 synthase
UDP-GlcNAc:lactosylceramide beta1,3-N-acetylglucosaminyltransferase
uridine diphosphoacetylglucosamine-lactosylceramide beta-acetylglucosaminyltransferase
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-
-
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beta1,3 N-acetylglucosaminyltransferase GlcNAc(beta1,3)Gal(beta1,4)Glc-ceramide synthase
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beta1,3 N-acetylglucosaminyltransferase GlcNAc(beta1,3)Gal(beta1,4)Glc-ceramide synthase
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UDP-GlcNAc:lactosylceramide beta1,3-N-acetylglucosaminyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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-
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-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
lacto-series glycosphingolipids biosynthesis, neolacto-series glycosphingolipids biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide 3-beta-N-acetylglucosaminyltransferase
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CAS REGISTRY NUMBER
COMMENTARY
83682-80-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-(1-4)-beta-D-glucosyl-(1-1)-ceramide
UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-phenyl-C14H29
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-phenyl-C14H29
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Substrates: synthetic substrate, 75% specific activity compared to lactosylceramide
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP-N-acetyl-D-glucosamine + Galbeta(1->4)GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->4)Manbeta1->3[Galbeta1->4GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->6)Manbeta1->6]Manbeta1->4GlcNAc
?
-
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-beta-N-acetyl-D-galactosaminyl-1,4-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: best substrate
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: acceptor substrate specificity, overview
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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Substrates: i.e. lactosylceramide
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: i.e. lactosylceramide
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: acceptor substrate is fluorescently labeled with 2-aminobenzamid or 2-pyridylamine
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: best substrate
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: acceptor substrate specificity, overview
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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Substrates: i.e. lactosylceramide
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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Substrates: i.e. lactosylceramide
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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Substrates: i.e. lactosylceramide
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: acceptor substrate is fluorescently labeled with 2-aminobenzamid or 2-pyridylamine
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: -
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: ganglioside metabolism and pattern
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: biosynthetic pathway of lactoseries and ganglioseries
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: may play a key role in regulating the level of various types of lactoseries tumor-associated antigens with the lacto type 1 or 2 chain
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: glycolipid biosynthesis
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: glycolipid biosynthesis
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: biosynthetic pathway of lactoseries and ganglioseries
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: enzyme is active at the branching point of lactosylceramide metabolism, directs lactosylceramide into the glycolipids of the lacto-series
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
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Substrates: glycolipid biosynthesis
Products: -
Products: -
?
additional information
?
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Substrates: the enzyme is expressed in two distinct phases during the zebrafish embryogenesis. The early phase extends from late blastulation to the completion of epiboly, during which the expression occurs in the superficial layer of the embryos. The second phase of expression starts during mid-segmentation and persists until day 3, during which the expression occurs prominently in the developing lens. Hedgehog signaling is required for the Lc3 synthase expression in embryonic lens
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is expressed in two distinct phases during the zebrafish embryogenesis. The early phase extends from late blastulation to the completion of epiboly, during which the expression occurs in the superficial layer of the embryos. The second phase of expression starts during mid-segmentation and persists until day 3, during which the expression occurs prominently in the developing lens. Hedgehog signaling is required for the Lc3 synthase expression in embryonic lens
Products: -
Products: -
?
additional information
?
-
-
Substrates: beta3Gn-T2 associates with beta3Gn-T8 in vivo
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme synthesizes the key intermediate lactosyltriosylceramide for lacto- and neolactoglycolipids. The large increase in the activity of Lc3 synthase appears to regulate the levels of other neolacto glycolipids, such as Lc3cer, nLc4Cer, nLc6Cer, disialosyl-nLc4Cer and Le(x)-glycolipids
Products: -
Products: -
?
additional information
?
-
-
Substrates: Lc3 synthase utilizes a variety of galactose-terminated glycolipids as acceptors by establishing a glycosidic bond in the beta-1,3-linkage to GlcNaAc to extend the lacto- and neolacto-series gangliosides
Products: -
Products: -
?
additional information
?
-
-
Substrates: Lc3 synthase utilizes a variety of galactose-terminated glycolipids as acceptors by establishing a glycosidic bond in the beta-1,3-linkage to GlcNaAc to extend the lacto- and neolacto-series gangliosides
Products: -
Products: -
?
additional information
?
-
-
Substrates: essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids
Products: -
Products: -
?
additional information
?
-
Substrates: essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: ganglioside metabolism and pattern
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: biosynthetic pathway of lactoseries and ganglioseries
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: may play a key role in regulating the level of various types of lactoseries tumor-associated antigens with the lacto type 1 or 2 chain
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: glycolipid biosynthesis
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: glycolipid biosynthesis
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: biosynthetic pathway of lactoseries and ganglioseries
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: enzyme is active at the branching point of lactosylceramide metabolism, directs lactosylceramide into the glycolipids of the lacto-series
Products: -
Products: -
?
UDP-N-acetyl-D-glucosamine + lactosylceramide
UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
Substrates: glycolipid biosynthesis
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme is expressed in two distinct phases during the zebrafish embryogenesis. The early phase extends from late blastulation to the completion of epiboly, during which the expression occurs in the superficial layer of the embryos. The second phase of expression starts during mid-segmentation and persists until day 3, during which the expression occurs prominently in the developing lens. Hedgehog signaling is required for the Lc3 synthase expression in embryonic lens
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is expressed in two distinct phases during the zebrafish embryogenesis. The early phase extends from late blastulation to the completion of epiboly, during which the expression occurs in the superficial layer of the embryos. The second phase of expression starts during mid-segmentation and persists until day 3, during which the expression occurs prominently in the developing lens. Hedgehog signaling is required for the Lc3 synthase expression in embryonic lens
Products: -
Products: -
?
additional information
?
-
-
Substrates: beta3Gn-T2 associates with beta3Gn-T8 in vivo
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme synthesizes the key intermediate lactosyltriosylceramide for lacto- and neolactoglycolipids. The large increase in the activity of Lc3 synthase appears to regulate the levels of other neolacto glycolipids, such as Lc3cer, nLc4Cer, nLc6Cer, disialosyl-nLc4Cer and Le(x)-glycolipids
Products: -
Products: -
?
additional information
?
-
-
Substrates: Lc3 synthase utilizes a variety of galactose-terminated glycolipids as acceptors by establishing a glycosidic bond in the beta-1,3-linkage to GlcNaAc to extend the lacto- and neolacto-series gangliosides
Products: -
Products: -
?
additional information
?
-
-
Substrates: Lc3 synthase utilizes a variety of galactose-terminated glycolipids as acceptors by establishing a glycosidic bond in the beta-1,3-linkage to GlcNaAc to extend the lacto- and neolacto-series gangliosides
Products: -
Products: -
?
additional information
?
-
-
Substrates: essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids
Products: -
Products: -
?
additional information
?
-
Substrates: essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mn2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
12-O-tetradecanoylphorbol-13-acetate
down-regulation of enzyme expression
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
retinoic acid
-
activates enzyme activity, leads to developmental malformations of the embryos
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Breast Neoplasms
Oncodevelopmental expression of--GlcNAc beta 1-6Man alpha 1-6Man beta 1--branched asparagine-linked oligosaccharides in murine tissues and human breast carcinomas.
Carcinoma
Helicobacter pylori induces beta3GnT5 in human gastric cell lines, modulating expression of the SabA ligand sialyl-Lewis x.
Carcinoma, Hepatocellular
Elevated activity of N-acetylglucosaminyltransferase V in human hepatocellular carcinoma.
Glioma
GMab-1, a high-affinity anti-3'-isoLM1/3',6'-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice.
Infections
Helicobacter pylori induces beta3GnT5 in human gastric cell lines, modulating expression of the SabA ligand sialyl-Lewis x.
Infections
Pathogen blocks host death receptor signalling by arginine GlcNAcylation of death domains.
Neoplasms
Beta 1-6 branching of Asn-linked oligosaccharides is directly associated with metastasis.
Neoplasms
Strong antibody reaction against glycosphingolipids injected in liposome-embedded forms in beta3GN-T5 knockout mice.
Neuroblastoma
Identification and characterization of three novel beta 1,3-N-acetylglucosaminyltransferases structurally related to the beta 1,3-galactosyltransferase family.
Salmonella Infections
Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.035
D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
cerebellum microsomes
0.42
Galbeta(1->4)GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->4)Manbeta1->3[Galbeta1->4GlcNAcbeta1->2(Galbeta1->4GlcNAcbeta1->6)Manbeta1->6]Manbeta1->4GlcNAc
-
betaGn3-T2
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.00121
0.00123
0.00174
0.0021
0.00254
additional information
0.00123
recombinant enzyme, substrate lD-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
0.00123
recombinant enzyme, substrate lD-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
0.00174
recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
0.00174
recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
0.0021
recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
0.0021
recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-(sialyl-alpha-2,8-sialyl-alpha-2,3)-D-galactosyl-1,4-beta-D-glucosylceramide
0.00254
recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
0.00254
recombinant enzyme, substrate D-galactosyl-1,4-beta-N-acetyl-D-galactosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
additional information
-
activity in different cell lines of normal colonic mucosa and colonic adenocarcinoma
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.2
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
the enzyme is expressedin two distinct phases during the zebrafish embryogenesis. The early phase extends from late blastulation to the completion of epiboly, during which the expression occurs in the superficial layer of the embryos. The second phase of expression starts during mid-segmentation and persists until day 3, during which the expression occurs prominently in the developing lens. Hedgehog signaling is required for the Lc3 synthase expression in embryonic lens
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Daoy and D341 Med, cultured both in vitro and as xenografts in nude mice
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additional information
distinct cellular layers: Purkinje cell layer, molecular cell layer, granule cell layer + white matter
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distinct cellular layers: Purkinje cell layer, molecular cell layer, granule cell layer + white matter
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the enzyme is expressed in two distinct phases during the zebrafish embryogenesis. The early phase extends from late blastulation to the completion of epiboly, during which the expression occurs in the superficial layer of the embryos. The second phase of expression starts during mid-segmentation and persists until day 3, during which the expression occurs prominently in the developing lens
brenda
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E17 fetus, both head and body show strong expression of Lc3 synthase in wild-type (Wt/Wt) and heterozygous (Wt/KO) mice
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E17 fetus, both head and body show strong expression of Lc3 synthase in wild-type (Wt/Wt) and heterozygous (Wt/KO) mice
-
brenda
additional information
transcript content and activity in diverse tumour cell lines, overview, nearly undetectable content in leukemic cell lines
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additional information
-
transcript content and activity in diverse tumour cell lines, overview, nearly undetectable content in leukemic cell lines
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additional information
-
enzyme levels in brain regions during pre- and postnatal brain development
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additional information
enzyme levels in brain regions during pre- and postnatal brain development
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additional information
-
retinoic acid activates the enzyme and leads to developmental malformations of the embryos, overview
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
metabolism
physiological function
evolution
-
Lc3 synthase is a member of the beta-1,3-N-glycosyltransferase superfamily
evolution
-
Lc3 synthase is a member of the beta-1,3-N-glycosyltransferase superfamily
-
malfunction
-
multiple phenotypic changes in mice after knockout of the B3gnt5 gene, encoding Lc3 synthase, detailed overview. B3gnt5 gene knockout mutant mice appear normal in the embryonic stage and, if they survive delivery, remain normal during early life, but about 9% develop early-stage growth retardation, 11% die postnatally in less than 2 months, and adults tend to die in 5-15 months, demonstrating splenomegaly and notably enlarged lymph nodes. Without lacto-neolacto series gangliosides, both homozygous and heterozygous mice gradually display fur loss or obesity, and breeding mice demonstrate reproductive defects. B3gnt5 gene knockout disrupts the functional integrity of B cells with a decrease in B-cell numbers in the spleen, germinal center disappearance, and less efficiency to proliferate in hybridoma fusion
malfunction
-
multiple phenotypic changes in mice after knockout of the B3gnt5 gene, encoding Lc3 synthase, detailed overview. B3gnt5 gene knockout mutant mice appear normal in the embryonic stage and, if they survive delivery, remain normal during early life, but about 9% develop early-stage growth retardation, 11% die postnatally in less than 2 months, and adults tend to die in 5-15 months, demonstrating splenomegaly and notably enlarged lymph nodes. Without lacto-neolacto series gangliosides, both homozygous and heterozygous mice gradually display fur loss or obesity, and breeding mice demonstrate reproductive defects. B3gnt5 gene knockout disrupts the functional integrity of B cells with a decrease in B-cell numbers in the spleen, germinal center disappearance, and less efficiency to proliferate in hybridoma fusion
-
metabolism
-
Lc3 synthase acts within the gangliosynthesis pathways, overview
-
physiological function
-
Lc3 synthase is a key enzyme in lacto-neolacto ganglioside synthesis, it exhibits strong activity in transferring GlcNAc to the lactosylceramide for lacto-neolacto-chain ganglioside biosynthesis, regulated during embryonic development, especially during brain morphogenesis
physiological function
-
Lc3 synthase is a key enzyme in lacto-neolacto ganglioside synthesis, it exhibits strong activity in transferring GlcNAc to the lactosylceramide for lacto-neolacto-chain ganglioside biosynthesis, regulated during embryonic development, especially during brain morphogenesis
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). B3G5A_XENLA
377
1
43635
Swiss-Prot
other Location (Reliability: 1)
B3G5A_DANRE
379
0
43600
Swiss-Prot
other Location (Reliability: 3)
A0A673MWF0_9TELE
796
0
92518
TrEMBL
other Location (Reliability: 4)
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
generation of Lc3 synthase gene B3gnt5-deficient knockout mice, phenotypes, overview
additional information
-
generation of Lc3 synthase gene B3gnt5-deficient knockout mice, phenotypes, overview
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination, functional expression in Spodoptera frugiperda Sf9 cells via baculovirus infection
DNA sequence determination, functional expression in Spodoptera frugiperda Sf9 cells via baculovirus infection
expression in Escherichia coli, overexpression of beta3GnT5 in MNK-45 cells and AGS cells, transfection with lipofectamine
-
partial sequence, not a full length clone, DNA sequence determination
soluble expression as FLAG-tagged protein in Spodoptera frugiperda Sf21 cells via baculovirus infection
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
-
adhesion of Helicobacter pylori to epithelial cells is mediated by bacterial adhesins that bind carbohydrate ligands expressed by the epithelial gastric cells, bea3GnT5 transferase induction is specific to infection with highly pathogenic strains
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gottfries, J.; Percy, A.K.; Mansson, J.E.; Fredman, P.; Wikstrand, C.J.; Friedman, H.S.; Bigner, D.D.; Svennerholm, L.
Glycolipids and glycosyltransferases in permanent cell lines established from human medulloblastomas
Biochim. Biophys. Acta
1081
253-261
1991
Homo sapiens
brenda
Holmes, E.H.; Hakomori, S.i.; Ostrander, G.K.
Synthesis of type 1 and 2 lacto series glycolipid antigens in human colonic adenocarcinoma and derived cell lines is due to activation of a normally unexpressed beta 1-3N-acetylglucosaminyltransferase
J. Biol. Chem.
262
15649-15658
1987
Homo sapiens
brenda
Chou, D.K.H.; Jungalwala, F.B.
N-acetylglucosaminyltransferase regulates the expression of neolactoglycolipids including sulfoglucuronylglycolipids in the developing nervous system
J. Biol. Chem.
268
21727-21733
1993
Rattus norvegicus
brenda
Rossi, F.; Gornati, R.; Rizzo, A.M.; Venturini, L.; Bernadini, G.; Berra, B.
Glycolipid glycosyltransferase activities during early development of Xenopus: effect of retinoic acid
Cell Biol. Int.
23
91-95
1999
Xenopus sp.
brenda
Chou, D.K.H.; Jungalwala, F.B.
N-acetylglucosaminyl Transferase regulates the expression of sulfoglucuronyl glycolipids in specific cell types in cerebellum during development
J. Biol. Chem.
271
28868-28874
1996
Mus musculus, Rattus norvegicus
brenda
Togayachi, A.; Akashima, T.; Ookubo, R.; Kudo, T.; Nishihara, S.; Iwasaki, H.; Natsume, A.; Mio, H.; Inokuchi, J.i.; Irimura, T.; Sasaki, K.; Narimatsu, H.
Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T5), an essential enzyme for the expression of HNK-1 and lewis X epitopes on glycolipids
J. Biol. Chem.
276
22032-22040
2001
Homo sapiens (Q9BYG0), Homo sapiens, Rattus norvegicus, Rattus norvegicus (Q99NB2)
brenda
Henion, T.R.; Zhou, D.; Wolfer, D.P.; Jungalwala, F.B.; Hennet, T.
Cloning of a mouse beta1,3 N-acetylglucosaminyltransferase GlcNAc(beta1,3)Gal(beta1,4)Glc-ceramide synthase gene encoding the key regulator of lacto-series glycolipid biosynthesis
J. Biol. Chem.
276
30261-30269
2001
Homo sapiens (Q9BYG0), Mus musculus (Q8BGY6), Mus musculus, no activity in Spodoptera frugiperda
brenda
Cao, Y.; Zhao, J.; Wang, Y.; Meng, A.
Expression of zebrafish Lc3 synthase gene in embryonic lens requires Hedgehog signaling
Dev. Dyn.
228
308-312
2003
Danio rerio (Q7T3S5), Danio rerio
brenda
Chou, D.K.H.; Henion, T.R.; Jungalwala, F.B.
Regulation of expression of sulfoglucuronyl carbohydrate (HNK-1), amphoterin and RAGE in retinoic acid-differentiated P19 embryonal carcinoma cells
J. Neurochem.
86
917-931
2003
Mus musculus
brenda
Marcos, N.T.; Magalhaes, A.; Ferreira, B.; Oliveira, M.J.; Carvalho, A.S.; Mendes, N.; Gilmartin, T.; Head, S.R.; Figueiredo, C.; David, L.; Santos-Silva, F.; Reis, C.A.
Helicobacter pylori induces beta 3GnT5 in human gastric cell lines, modulating expression of the SabA ligand sialyl-Lewis x
J. Clin. Invest.
118
2325-2336
2008
Homo sapiens
brenda
Seko, A.; Yamashita, K.
Activation of beta1,3-N-acetylglucosaminyltransferase-2 (beta3Gn-T2) by beta3Gn-T8: Possible involvement of beta3Gn-T8 in increasing poly-N-acetyllactosamine chains in differentiated HL-60 cells
J. Biol. Chem.
283
33094-33100
2008
Homo sapiens
brenda
Kuan, C.T.; Chang, J.; Mansson, J.E.; Li, J.; Pegram, C.; Fredman, P.; McLendon, R.E.; Bigner, D.D.
Multiple phenotypic changes in mice after knockout of the B3gnt5 gene, encoding Lc3 synthase - a key enzyme in lacto-neolacto ganglioside synthesis
BMC Dev. Biol.
10
114
2010
Mus musculus, Mus musculus C57BL/6
brenda
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