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Information on EC 2.4.1.203 - trans-zeatin O-beta-D-glucosyltransferase
for references in articles please use BRENDA:EC2.4.1.203
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.203 trans-zeatin O-beta-D-glucosyltransferase
IUBMB Comments
Unlike EC 2.4.1.215, cis-zeatin O-β-D-glucosyltransferase, UDP-D-xylose can also act as donor (cf. EC 2.4.2.40, zeatin O-β-D-xylosyltransferase).
The enzyme appears in viruses and cellular organisms
- 2.4.1.203
- seedling
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planta
-
n-glucosides
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n-glucosylation
- glycosyltransferases
- anthocyanin
- drought
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abscisic
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brassinosteroids
- chlorophyll
- cis-zeatin
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postgermination
-
cheap
- brassinolide
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phytohormones
- rhodiola
- salidroside
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cytokinin-related
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overexpressors
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diphosphate-dependent
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br-deficiency
- agriculture
Synonyms
ugt76c2, ugt73c5, ugt76c1, ugt85a1, trans-zeatin o-glucosyltransferase, ugt73c1, glucosyltransferase ugt85a1, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
glucosyltransferase UGT85A1
glucosyltransferase, uridine diphosphoglucose-zeatin O-
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trans-zeatin O-beta-D-glucosyltransferase
trans-zeatin O-glucosyltransferase
tZOG
UGT85A1
uridine diphosphoglucose-zeatin O-glucosyltransferase
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zeatin glycosyltransferase
zeatin O-beta-D-glucosyltransferase
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zeatin O-glucosyltransferase
ZOG1
CK-specific UGT
zeatin O-glucosyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + trans-zeatin = UDP + O-beta-D-glucosyl-trans-zeatin
localisation of site determining the substrate specificity for the UDP-sugar donors
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UDP-glucose + trans-zeatin = UDP + O-beta-D-glucosyl-trans-zeatin
unlike EC 2.4.1.215, cis-zeatin O-beta-D-glucosyltransferase, UDP-xylose can also act as donor
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
cytokinin-O-glucosides biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:trans-zeatin O-beta-D-glucosyltransferase
Unlike EC 2.4.1.215, cis-zeatin O-beta-D-glucosyltransferase, UDP-D-xylose can also act as donor (cf. EC 2.4.2.40, zeatin O-beta-D-xylosyltransferase).
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CAS REGISTRY NUMBER
COMMENTARY
123644-76-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 2-[3-(but-3-yn-1-yl)-3H-diazirin-3-yl]ethyl 13alpha-hydroxy-8alpha,10alpha-kaur-16-en-18-oate
UDP + ?
Substrates: -
Products: -
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?
UDP-alpha-D-glucose + prop-2-yn-1-yl 13alpha-hydroxy-8alpha,10alpha-kaur-16-en-18-oate
UDP + ?
Substrates: determination and analysis of the substrate binding structure of UGT73C1, model, overview
Products: -
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?
UDP-D-glucose + cis-zeatin
UDP + O-beta-D-glucosyl-cis-zeatin
Substrates: -
Products: -
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?
UDP-D-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
Substrates: -
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?
UDP-glucose + m-topolin
UDP + O-beta-D-glucosyl-m-topolin
Substrates: 23% of the activity with trans-zeatin
Products: -
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?
UDP-glucose + steviol
UDP + ?
Substrates: determination and analysis of the substrate binding structure of UGT73C1, model, overview
Products: -
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?
UDP-glucose + trans-zeatin
UDP + 9-beta-D-glucosyl-trans-zeatin
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Substrates: -
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?
UDP-galactose + trans-zeatin
UDP + O-galactosylzeatin
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Substrates: -
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?
UDP-galactose + trans-zeatin
UDP + O-galactosylzeatin
Substrates: -
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Products: -
?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
Substrates: -
Products: -
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?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
-
Substrates: -
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Products: -
?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
-
Substrates: -
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Products: -
?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
-
Substrates: -
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?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
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Substrates: -
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Products: -
?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
Substrates: -
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?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
Substrates: -
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?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
Substrates: -
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?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
-
Substrates: -
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Products: -
?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
Substrates: -
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?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
Substrates: -
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UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
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Substrates: UDP-glucose is the best donor substrate
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UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
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Substrates: cis-zeatin, ribosylzeatin, and dihydrozeatin are no substrates
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?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
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Substrates: specific for trans-zeatin
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?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
Substrates: -
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Products: -
?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
-
Substrates: -
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?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
-
Substrates: -
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?
UDP-glucose + zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
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Substrates: important in regulating the level of active cytokinin, i.e. zeatin, in plant tissues
Products: -
Products: -
?
UDP-glucose + zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
Substrates: important in regulating the level of active cytokinin, i.e. zeatin, in plant tissues
Products: -
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?
UDP-xylose + dihydrozeatin
UDP + O-beta-D-xylosyldihydrozeatin
Substrates: -
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?
UDP-xylose + dihydrozeatin
UDP + O-beta-D-xylosyldihydrozeatin
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Substrates: converts dihydrozeatin exclusively with UDP-D-xylose as donor substrates
Products: -
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?
UDP-xylose + trans-zeatin
UDP + O-xylosylzeatin
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Substrates: -
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?
UDP-xylose + trans-zeatin
UDP + O-xylosylzeatin
Substrates: -
Products: -
Products: -
?
additional information
?
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Substrates: UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
Products: -
Products: -
?
additional information
?
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Substrates: UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
Products: -
Products: -
?
additional information
?
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Substrates: UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
Products: -
Products: -
?
additional information
?
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Substrates: UGT85A1, UGT73C5, and UGT73C1 recognize trans-zeatin and dihydrozeatin, which have an available hydroxyl group for glucosylation and form the O-glucosides
Products: -
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?
additional information
?
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additional information
Substrates: enzyme UGT85A1 is also active with cis-zeatin
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additional information
?
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Substrates: enzyme UGT85A1 is also active with cis-zeatin
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additional information
?
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Substrates: enzyme UGT85A1 is also active with cis-zeatin
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additional information
?
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additional information
Substrates: enzyme UGT85A1 might also active with cis-zeatin forming 9-O-glucosides, cf. EC 2.4.1.215
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additional information
?
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Substrates: enzyme UGT85A1 might also active with cis-zeatin forming 9-O-glucosides, cf. EC 2.4.1.215
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additional information
?
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Substrates: enzyme UGT85A1 might also active with cis-zeatin forming 9-O-glucosides, cf. EC 2.4.1.215
Products: -
Products: -
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additional information
?
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Substrates: the enzyme is also active on steviol. Chemical product analysis reveals that the enzyme forms 13-O-beta-D-glucosyl-steviol and not 19-O-beta-D-glucosyl-steviol
Products: -
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additional information
?
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Substrates: the enzyme is also active on steviol. Chemical product analysis reveals that the enzyme forms 13-O-beta-D-glucosyl-steviol and not 19-O-beta-D-glucosyl-steviol
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additional information
?
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Substrates: no activity with o-hydroxythidiazuron
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?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
additional information
Substrates: -
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + trans-zeatin
UDP + 9-beta-D-glucosyl-trans-zeatin
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
additional information
Substrates: -
Products: -
Products: -
?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
Substrates: -
Products: -
Products: -
?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + trans-zeatin
UDP + 7-beta-D-glucosyl-trans-zeatin
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
Substrates: -
Products: -
Products: -
?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
Substrates: -
Products: -
Products: -
?
UDP-glucose + trans-zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
-
Substrates: important in regulating the level of active cytokinin, i.e. zeatin, in plant tissues
Products: -
Products: -
?
UDP-glucose + zeatin
UDP + O-beta-D-glucosyl-trans-zeatin
Substrates: important in regulating the level of active cytokinin, i.e. zeatin, in plant tissues
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
steady state kinetic analysis of UGT73C1, non-linear data fitting of Michaelis-Menten equation
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additional information
additional information
-
steady state kinetic analysis of UGT73C1, non-linear data fitting of Michaelis-Menten equation
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain L. cv. Wisconsin, wild type and mutants over-expressing trans-zeatin O-glucosyltransferase gene under 35S or SAG12 promoters
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brenda
analysis of CK glucosides formation during main developmental transition phases
additional information
-
brenda
Nicotiana tabacum expressing a ZOG1 gene, encoding trans-zeatin-O-glucosyltransferase from Phaseolus lunatus. When a ZOG1 gene is constitutively expressed in tabacco: Cytokinin-O-glucosides accumulate in leaves. The level of t-ZOG is two orders of magnitude higher. Higher content of the predominant cytokinin forms bases and ribosides and of cytokinin phosphates in ZOG1 chloroplasts. Auxin content in the leaves is 2fold higher. Auxin and abscisic acid contens are higher in ZOG1 chloroplasts than in wild type chloroplasts. The chloroplasts ZOG1 transforms contain more starch inclusion than wild type W38 chloroplasts.
-
-
brenda
trans-zeatin O-beta-D-glucosyltransferase gene is overexpressed in maize
SwissProt
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
UGT85A1 is specifically expressed in senescent leaves
brenda
UGT85A1 is mainly expressed in the early seedlings, spatial-temporal expression patterns, overview
brenda
-
UGT85A1 is mainly expressed in the early seedlings, spatial-temporal expression patterns, overview
-
brenda
additional information
UGT85A1 is specifically expressed in senescent leaves
brenda
additional information
additional information
analysis of CK glucosides formation during main developmental transition phases
brenda
additional information
analysis of CK glucosides formation during main developmental transition phases
brenda
additional information
analysis of CK glucosides formation during main developmental transition phases
brenda
additional information
additional information
UGT76C2 is activated rapidly in response to exogenously applied cytokinin. Analysis of CK glucosides formation during main developmental transition phases
brenda
additional information
UGT76C2 is activated rapidly in response to exogenously applied cytokinin. Analysis of CK glucosides formation during main developmental transition phases
brenda
additional information
UGT76C2 is activated rapidly in response to exogenously applied cytokinin. Analysis of CK glucosides formation during main developmental transition phases
brenda
additional information
very low expression level in vegetative tissues
brenda
additional information
-
very low expression level in vegetative tissues
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
in silico localization analysis
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
physiological function
malfunction
malfunction
the accumulation level of the trans-zeatin O-glucosides is significantly increased in UGT85A1 overexpressing transgenic Arabidopsis thaliana, while other forms of cytokinins occur in concentrations similar to the wild-type. When treated with exogenously applied trans-zeatin, UGT85A1 overexpressing Arabidopsis thaliana plants show much less sensitivity to trans-zeatin in primary root elongation and lateral root formation. The chlorophyll content of detached leaves of transgenic Arabidopsis thaliana plants is much lower than wild-type, phenotype, overview
malfunction
a ugt76c1-1 loss-of-function mutant shows some specificity toward cis-zeatin (cZ) in contrast to the wild-type enzyme. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c1-1 mutant before and after cytokinin treatment, overview
malfunction
a ugt76c2-1 loss-of-function mutant possesses extremely diminished CK N-glucosides levels compared to wild-type. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c2-1 mutant before and after cytokinin treatment, overview
malfunction
UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type. Besides tZOG, a broader range of CK glucosides is decreased in ugt85a1-1 mutant. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt85a1-1 mutant, overview
malfunction
-
the accumulation level of the trans-zeatin O-glucosides is significantly increased in UGT85A1 overexpressing transgenic Arabidopsis thaliana, while other forms of cytokinins occur in concentrations similar to the wild-type. When treated with exogenously applied trans-zeatin, UGT85A1 overexpressing Arabidopsis thaliana plants show much less sensitivity to trans-zeatin in primary root elongation and lateral root formation. The chlorophyll content of detached leaves of transgenic Arabidopsis thaliana plants is much lower than wild-type, phenotype, overview
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metabolism
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in general, trans-zeatin (tZ) and N6-(DELTA2-isopentenyl)adenine (iP)-types predominate in the spectrum of cytokinins in Brassica napus, with N7-glucosides, namely tZ-N7-glucoside (tZ7G) and iPN7-glucoside (iP7G), representing the most abundant forms. Leptosphaeria maculans is a phytopathogenic fungus. It can produce cytokinins (CKs) in vitro and its CK profile differs from that in tissue of its host Brassica napus. At 7 dpi, CK levels remains statistically unaffected by the infection. With the progression of the infection, the levels of most CK forms increase at 10 dpi. The total CK content increases to 150% compared to mock-infected samples. The highest (240%) increase is observed for cis-zeatin (cZ)-type CKs. All of the detected cZ-type derivatives are induced by infection at 10 dpi, with the free cZ and cZ-N7-glucoside (cZ7G) reaching the highest concentrations. Infection with Leptosphaeria maculans modifies significantly the content of CKs in oilseed rape cotyledon leaves. Cytokinin spectrum and content, overview
metabolism
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the fungus Leptosphaeria maculans strain JN3 contains both cis- and trans-zeatin O-glucosyltransferases (EC 2.4.1.215 and EC 2.4.1.203), and a cis-trans-isomerase, that are all involved in the cytokinin (CK) metabolism of the pathogenic fungus. Among the glucosides, glucosides of trans-zeatin (tZ) and N7-glucosides (iP7G) are detected, whereas N9-glucosides are mostly missing in the mycelium. The most abundant metabolite of the tZ-type cytokinins is O-beta-D-glucosyl-trans-zeatin (tZOG). The tZ feeding increases cis-zeatin (cZ). At 9 days, the sum of total CKs in the mycelium increases compared to 7 days, mainly due to the increase of cis-zeatin (cZ)-type CKs, the free cZ especially being the highly predominant CK derivative. Leptosphaeria maculans can produce CKs in vitro and its CK profile differs from that of its host Brassica napus tissue. Leptosphaeria maculans metabolizes exogenously added CKs (iP, tZ, cZ, all at 0.001 mM). Cytokinin spectrum and content, overview. The cis-trans-isomerase performs zeatin cis-trans isomerisation in Leptosphaeria maculans
metabolism
-
the fungus Leptosphaeria maculans strain JN3 contains both cis- and trans-zeatin O-glucosyltransferases (EC 2.4.1.215 and EC 2.4.1.203), and a cis-trans-isomerase, that are all involved in the cytokinin (CK) metabolism of the pathogenic fungus. Among the glucosides, glucosides of trans-zeatin (tZ) and N7-glucosides (iP7G) are detected, whereas N9-glucosides are mostly missing in the mycelium. The most abundant metabolite of the tZ-type cytokinins is O-beta-D-glucosyl-trans-zeatin (tZOG). The tZ feeding increases cis-zeatin (cZ). At 9 days, the sum of total CKs in the mycelium increases compared to 7 days, mainly due to the increase of cis-zeatin (cZ)-type CKs, the free cZ especially being the highly predominant CK derivative. Leptosphaeria maculans can produce CKs in vitro and its CK profile differs from that of its host Brassica napus tissue. Leptosphaeria maculans metabolizes exogenously added CKs (iP, tZ, cZ, all at 0.001 mM). Cytokinin spectrum and content, overview. The cis-trans-isomerase performs zeatin cis-trans isomerisation in Leptosphaeria maculans
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metabolism
-
the fungus Leptosphaeria maculans strain JN3 contains both cis- and trans-zeatin O-glucosyltransferases (EC 2.4.1.215 and EC 2.4.1.203), and a cis-trans-isomerase, that are all involved in the cytokinin (CK) metabolism of the pathogenic fungus. Among the glucosides, glucosides of trans-zeatin (tZ) and N7-glucosides (iP7G) are detected, whereas N9-glucosides are mostly missing in the mycelium. The most abundant metabolite of the tZ-type cytokinins is O-beta-D-glucosyl-trans-zeatin (tZOG). The tZ feeding increases cis-zeatin (cZ). At 9 days, the sum of total CKs in the mycelium increases compared to 7 days, mainly due to the increase of cis-zeatin (cZ)-type CKs, the free cZ especially being the highly predominant CK derivative. Leptosphaeria maculans can produce CKs in vitro and its CK profile differs from that of its host Brassica napus tissue. Leptosphaeria maculans metabolizes exogenously added CKs (iP, tZ, cZ, all at 0.001 mM). Cytokinin spectrum and content, overview. The cis-trans-isomerase performs zeatin cis-trans isomerisation in Leptosphaeria maculans
-
physiological function
glucosyltransferase UGT85A1 is another zeatin O-glucosyltransferase with a preference for trans-zeatin. It influences trans-zeatin homeostasis and trans-zeatin responses likely through O-glucosylation, regulation, overview
physiological function
cytokinin-specific UGTs possess different physiological roles in Arabidopsis thaliana and serve as a fine-tuning mechanism of active CK levels in cytosol
physiological function
-
glucosyltransferase UGT85A1 is another zeatin O-glucosyltransferase with a preference for trans-zeatin. It influences trans-zeatin homeostasis and trans-zeatin responses likely through O-glucosylation, regulation, overview
-
malfunction
additional information
a ugt76c1-1 loss-of-function mutant shows some specificity toward cis-zeatin (cZ) in contrast to the wild-type enzyme. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c1-1 mutant before and after cytokinin treatment, overview
malfunction
additional information
a ugt76c2-1 loss-of-function mutant possesses extremely diminished CK N-glucosides levels compared to wild-type. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt76c2-1 mutant before and after cytokinin treatment, overview
malfunction
additional information
UGT85A1 is expressed in wild-type but not in the ugt85a1-1 mutant. The T-RNA insertion ugt85a1-1 loss-of-function mutant plants show no altered phenotype compared to wild-type. Besides tZOG, a broader range of CK glucosides is decreased in ugt85a1-1 mutant. CK metabolism gene expression profiling reveals that activation of the CK degradation pathway serves as a general regulatory mechanism of disturbed CK homeostasis followed by decreased CK signaling in all UGT mutants. A specific regulation of CKX7, CKX1 and CKX2 is observed. Cytokinin content of 4-week-old seedlings of ugt85a1-1 mutant, overview
additional information
homology structure modelling of UGT73C1 using high resolution crystal structures of glycosyltransferases UGT72B1 (PDB ID 2VCH) and UGT74F2 (PDB ID 5V2K) as templates
additional information
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homology structure modelling of UGT73C1 using high resolution crystal structures of glycosyltransferases UGT72B1 (PDB ID 2VCH) and UGT74F2 (PDB ID 5V2K) as templates
physiological function
additional information
cytokinin-specific UGTs possess different physiological roles in Arabidopsis thaliana and serve as a fine-tuning mechanism of active CK levels in cytosol
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE