Information on EC 2.4.1.197 - high-mannose-oligosaccharide beta-1,4-N-acetylglucosaminyltransferase

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EC Tree 2 Transferases 2.4 Glycosyltransferases 2.4.1 Hexosyltransferases 2.4.1.197 high-mannose-oligosaccharide beta-1,4-N-acetylglucosaminyltransferase

IUBMB Comments

The activity of the intersecting mannose residue as acceptor is dependent on two other mannose residues attached by α-1,3 and α-1,6 links.

The expected taxonomic range for this enzyme is: Dictyostelium discoideum

Reaction Schemes

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UDP-N-acetyl-D-glucosamine

O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

UDP

O-mannopyranosyl-alpha-1,3-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-[N-acetylglucosamine-beta-1,4-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

Synonyms
udp-glcnac:oligosaccharide beta-n-acetylglucosaminyltransferase, more

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

acetylglucosamine-oligosaccharide acetylglucosaminyltransferase

acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-oligosaccharide

UDP-GlcNAc:oligosaccharide beta-N-acetylglucosaminyltransferase

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

Transfers an N-acetyl-D-glucosamine residue from UDP-N-acetyl-D-glucosamine to the 4-position of a mannose linked alpha-(1->6) to the core mannose of high-mannose oligosaccharides produced by Dictyostelium discoideum

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REACTION TYPE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

hexosyl group transfer

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SYSTEMATIC NAME

IUBMB Comments

UDP-N-acetyl-D-glucosamine:high-mannose-oligosaccharide 4-beta-N-acetylglucosaminyltransferase

The activity of the intersecting mannose residue as acceptor is dependent on two other mannose residues attached by alpha-1,3 and alpha-1,6 links.

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CAS REGISTRY NUMBER

COMMENTARY

123425-54-7

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

Reversibility
r=reversible
ir=irreversible
?=not specified

UDP-N-acetyl-D-glucosamine + (O-mannopyranosyl-alpha-1,3)-mannopyranosyl-beta-1,4-N-acetyl-glucosamine

UDP + O-mannopyranosyl-alpha-1,3-[N-acetyl-glucosamine-beta-1,4]-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

Dictyostelium discoideum

488873

Substrates: i.e. Man2GlcNAc, very low activity
Products: -

UDP-N-acetyl-D-glucosamine + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside

UDP + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranosyl-1,4-N-acetyl-D-glucosamine

2 entries

UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine

UDP + O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-(N-acetyl-D-glucosamine-beta-1,4)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

Dictyostelium discoideum

488873

Substrates: i.e. Man9GlcNAc oligosaccharide, enzyme catalyzes the transfer of N-acetyl-glucosamine to both alpha- and beta linked mannoses
Products: i.e. Man9GlcNAc oligosaccharide with an additional N-acetyl-D-glucosamine residue attached beta-1,4 to the mannose linked alpha-1,6 to the core mannose in an intersecting position

UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3-mannopyranosyl-alpha-methyl

Dictyostelium discoideum

488873

Substrates: i.e. Man2Me
Products: -

UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

UDP + O-mannopyranosyl-alpha-1,3-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-[N-acetylglucosamine-beta-1,4-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

Dictyostelium discoideum

488873

Substrates: i.e. Man5GlcNAc oligosaccharide, 20% of activity compared to Man9GlcNAc
Products: i.e. Man5GlcNAc oligosaccharide with an additional N-acetyl-D-glucosamine residue in an intersected and/or bisected position, so that either the core beta-mannose or the branching alpha-mannose residue accepts the transferred N-acetyl-D-glucosamine residue

UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine

UDP + O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6-(N-acetyl-D-glucosamine beta-1,4)]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine

Dictyostelium discoideum

488873

Substrates: i.e. Man3GlcNAc
Products: -

UDP-N-acetyl-D-glucosamine + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside

UDP + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranosyl-1,4-N-acetyl-D-glucosamine

Dictyostelium discoideum

488874

Substrates: -
Products: -

UDP-N-acetyl-D-glucosamine + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside

UDP + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranosyl-1,4-N-acetyl-D-glucosamine

Dictyostelium discoideum

488873

Substrates: trivial name Man3Me
Products: -

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Mn2+

Dictyostelium discoideum

stimulation

488873

additional information

Dictyostelium discoideum

neither Mg2+ nor Ca2+ can substitute for Mn2+

488874

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

dithiothreitol

Dictyostelium discoideum

strong inhibition in the presence of Mg2+ and Mn2+

488873

EDTA

Dictyostelium discoideum

strong

488873

EGTA

Dictyostelium discoideum

strong

488873

Mg2+

Dictyostelium discoideum

strong

488873

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1.42

methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside

Dictyostelium discoideum

intersected product

488873

0.65

O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine

Dictyostelium discoideum

i.e. Man9GlcNAc, intersected product

488873

18.52

O-mannopyranosyl-alpha-1,3-mannopyranosyl-alpha-methyl

Dictyostelium discoideum

intersected product

488873

19.48

O-mannopyranosyl-alpha-1,3-mannopyranosyl-beta-1,4-N-acetyl-glucosamine

Dictyostelium discoideum

bisected product

488873

0.3 - 0.43

O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

Dictyostelium discoideum

i.e. Man5GlcNAc, depending whether N-acetyl-D-glucosamine residue is intersected or bisected

488873

2.54

O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine

Dictyostelium discoideum

bisected product

488873

1.2

UDP-N-acetyl-D-glucosamine

Dictyostelium discoideum

cosubstrate Man9GlcNAc

488873

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.0000077

Dictyostelium discoideum

Man5GlcNAc, intersected

488873

0.0000129

Dictyostelium discoideum

Man9GlcNAc, intersected

488873

0.0000133

Dictyostelium discoideum

Man5GlcNAc, bisected

488873

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

6 - 6.9

Dictyostelium discoideum

488873

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pH RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

5.5 - 7.2

Dictyostelium discoideum

20% of maximal activity at pH 5.5, 70% of maximal activity at pH 7.2

488873

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2 entries

Dictyostelium discoideum

488874

Dictyostelium discoideum

assay at

488873

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

Dictyostelium discoideum

488873, 488874

brenda

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

Golgi membrane

Dictyostelium discoideum

integral membrane protein, at least the catalytic center is on the luminal site of the vesicles

139

brenda

Dictyostelium discoideum

16020

488874

brenda

membrane

Dictyostelium discoideum

membrane-associated

16020

488873

brenda

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Dictyostelium discoideum

75% decrease of activity after 2 min

488873

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

strain M4, partial

Dictyostelium discoideum

488873

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

488873

Sharkey, D.J.; Kornfeld, R.

Identification of an N-acetylglucosaminyltransferase in Dictyostelium discoideum that transfers an intersecting N-acetylglucosamine residue to high mannose oligosaccharides

J. Biol. Chem.

264

10411-10419

1989

Dictyostelium discoideum

2525124

brenda

488874

Capasso, J.M.; Capasso, A.; Kaplan, A.

Subcellular distribution of intersecting beta-N-acetylglucosaminyltransferase in Dictyostelium discoideum. A likely marker for the Golgi apparatus

Biochim. Biophys. Acta

1281

15-22

1996

Dictyostelium discoideum

8652599

brenda

Entries 1 - 20 of 2

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EXTERNAL LINKS (specific for EC-Number 2.4.1.197)

ExplorEnz (official portal for IUBMB Enzyme Nomenclature)

Expasy Enzyme Nomenclature Database

KEGG

SABIO-RK

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

Enzyme Nomenclature (alternative site)

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)

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