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IUBMB Comments Specific to 5β-spirostanols. Involved in the biosynthesis of plant saponins. Not identical with EC 2.4.1.173 (sterol 3β-glucosyltransferase) or EC 2.4.1.192 (nuatigenin 3β-glucosyltransferase).
Synonyms
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glucosyltransferase, uridine diphosphoglucose-sarsapogenin
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UDPglucose:(25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucosyltransferase
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UDP-glucose + (25S)-5beta-spirostan-3beta-ol = UDP + (25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
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hexosyl group transfer
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UDP-glucose:(25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucosyltransferase
Specific to 5beta-spirostanols. Involved in the biosynthesis of plant saponins. Not identical with EC 2.4.1.173 (sterol 3beta-glucosyltransferase) or EC 2.4.1.192 (nuatigenin 3beta-glucosyltransferase).
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UDPglucose + (25R)-5alpha-spirostan-3beta-ol
UDP + (25R)-5alpha-spirostan-3beta-ol 3-O-beta-D-glucoside
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Substrates: weak activity
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UDPglucose + (25R)-5alpha-spirostan-3beta-ol-12-one
UDP + (25R)-5alpha-spirostan-3beta-ol-12-one 3-O-beta-D-glucoside
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Substrates: weak activity
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UDPglucose + (25R)-5alpha-spirostane-3beta,6alpha-diol
UDP + (25R)-5alpha-spirostane-3beta,6alpha-diol 3-O-beta-D-glucoside
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Substrates: weak activity
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UDPglucose + (25R)-5beta-spirostan-3beta-ol
UDP + (25R)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
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Substrates: i.e. smilagenin
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UDPglucose + (25R)-spirost-5-en-3beta-ol
UDP + (25R)-spirost-5-en-3beta-ol 3-O-beta-D-glucoside
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Substrates: weak activity
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UDPglucose + (25S)-5beta-spirostan-3beta-ol
UDP + (25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
UDPglucose + 5alpha-cholestan-3beta-ol
UDP + 5alpha-cholestan-3beta-ol 3-O-beta-D-glucoside
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Substrates: weak activity
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UDPglucose + 5beta-cholestan-3beta-ol
UDP + 5beta-cholestan-3beta-ol 3-O-beta-D-glucoside
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Substrates: weak activity
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UDPglucose + stigmast-5-en-3beta-ol
UDP + stigmast-5-en-3beta-ol 3-O-beta-D-glucoside
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Substrates: weak activity
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UDPglucose + (25S)-5beta-spirostan-3beta-ol
UDP + (25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
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Substrates: i.e. sarsapogenin
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UDPglucose + (25S)-5beta-spirostan-3beta-ol
UDP + (25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
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Substrates: involved in the biosynthesis of plant saponins
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UDPglucose + (25S)-5beta-spirostan-3beta-ol
UDP + (25S)-5beta-spirostan-3beta-ol 3-O-beta-D-glucoside
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Substrates: involved in the biosynthesis of plant saponins
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EDTA
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1 mM, 43% inhibition
EGTA
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10 mM, 50% inhibition
PCMB
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0.01 mM, 94% inhibition
UDP
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1 mM, 89% inhibition
UMP
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1 mM, 51% inhibition
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7.2 - 9.9
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pH 7.2: about 40% of maximal activity, pH 9.9: about 60% of maximal activity
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brenda
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of 3-week-old plants
brenda
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brenda
Highest Expressing Human Cell Lines
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Gene Links
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Paczkowski, C.; Wojciechowski, Z.A.
The occurence of UDPG-dependent glucosyltransferase specific for sarsasapogenin in Asparagus officinalis
Phytochemistry
27
2743-2747
1988
Asparagus officinalis
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brenda
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