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IUBMB Comments Involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls.
Synonyms
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acetylmannosaminyltransferase, uridine diphosphoacetyl-mannosamineacetylglucosaminylpyrophosphorylundecaprenol
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N-acetylmannosamine transferase
N-acetylmannosaminyltransferase
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UDP-N-acetylmannosamine:N-acetylglucosaminyl diphosphorylundecaprenol N-acetylmannosaminyltransferase
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N-acetylmannosamine transferase
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N-acetylmannosamine transferase
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UDP-N-acetyl-alpha-D-mannosamine + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP-N-acetyl-alpha-D-mannosamine + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
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UDP-N-acetyl-alpha-D-mannosamine + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
the enzyme utilize a sequential mechanism in which both substrates bind prior to product release. The mechanism is not a rapid equilibrium ordered mechanism.
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hexosyl group transfer
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MetaCyc
poly(3-O-beta-D-glucopyranosyl-N-acetylgalactosamine 1-phosphate) wall teichoic acid biosynthesis, poly(glycerol phosphate) wall teichoic acid biosynthesis, poly(ribitol phosphate) wall teichoic acid biosynthesis I (B. subtilis), poly(ribitol phosphate) wall teichoic acid biosynthesis II (S. aureus), Salmonella enterica serotype O:54 O antigen biosynthesis
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UDP-N-acetyl-alpha-D-mannosamine:N-acetyl-alpha-D-glucosaminyldiphospho-ditrans,octacis-undecaprenol 4-beta-N-acetylmannosaminyltransferase (configuration-inverting)
Involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls.
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UDP-N-acetyl-alpha-D-mannosamine + 2-(acetylamino)-2-deoxy-1-O-(hydroxy[[hydroxy(tridecyloxy)phosphoryl]oxy]phosphoryl)-alpha-D-glucopyranose
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Substrates: -
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UDP-N-acetyl-alpha-D-mannosamine + 2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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Substrates: -
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UDP-N-acetyl-alpha-D-mannosamine + 2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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Substrates: -
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UDP-N-acetyl-alpha-D-mannosamine + 2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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Substrates: -
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UDP-N-acetyl-alpha-D-mannosamine + 2-(acetylamino)-2-deoxy-1-O-[[[[[(2Z)-3,7-dimethylocta-2,6-dien-1-yl]oxy](hydroxy)phosphoryl]oxy](hydroxy)phosphoryl]-alpha-D-glucopyranose
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Substrates: -
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UDP-N-acetyl-alpha-D-mannosamine + 2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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Substrates: -
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UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol
UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
additional information
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UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol
UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
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Substrates: -
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UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol
UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
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Substrates: -
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UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol
UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
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Substrates: -
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additional information
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Substrates: involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls
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additional information
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Substrates: involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls
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UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol
UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
additional information
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UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol
UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
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Substrates: -
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UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol
UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
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Substrates: -
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UDP-N-acetylmannosamine + N-acetylglucosaminyl diphosphoryl-undecaprenol
UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol
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Substrates: -
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additional information
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Substrates: involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls
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additional information
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Substrates: involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls
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(NH4)2SO4
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0.3-0.5 M: stimulation
MgCl2
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10 mM, highest stimulatory effect
NH4Cl
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0.3-0.5 M: stimulation
NaCl
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0.3-0.5 M: stimulation
NaCl
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the enzymatic activity is sensitive to NaCl concentration, with the highest activity at 250 mM
additional information
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activity does not improve with the addition of Mg2+ at concentrations up to 30 mM
additional information
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activity does not improve with the addition of Mn2+ at concentrations up to 30 mM
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2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-mannopyranosyl]-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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is an I-parabolic, S-parabolic noncompetitive inhibitor with respect ro UDP-ManNAc and an I-linear, S-parabolic noncompetitive inhibitor with respect to the acceptor 3 at nonsaturating levels
additional information
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activity does not decrease with the addition of EDTA
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glycerol
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25%: twofold increase of activity
Nonidet P-40
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0.1%: small stimulatory effect
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0.193
2-(acetylamino)-2-deoxy-1-O-(hydroxy[[hydroxy(tridecyloxy)phosphoryl]oxy]phosphoryl)-alpha-D-glucopyranose
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apparent Km at 600 microM UDP-ManNAc
0.03
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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apparent Km at 600 microM UDP-ManNAc
0.022
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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apparent Km at 600 microM UDP-ManNAc
0.035
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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apparent Km at 600 microM UDP-ManNAc
0.258
2-(acetylamino)-2-deoxy-1-O-[[[[[(2Z)-3,7-dimethylocta-2,6-dien-1-yl]oxy](hydroxy)phosphoryl]oxy](hydroxy)phosphoryl]-alpha-D-glucopyranose
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apparent Km at 600 microM UDP-ManNAc
0.0044
UDP-N-acetylmannosamine
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0.04 - 9.183
2-(acetylamino)-2-deoxy-1-O-(hydroxy[[hydroxy(tridecyloxy)phosphoryl]oxy]phosphoryl)-alpha-D-glucopyranose
10.25
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2E,6E,10E)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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apparent Kcat at 600 microM UDP-ManNAc
0.52 - 13.92
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
0.04 - 8.617
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
0.866 - 6.08
2-(acetylamino)-2-deoxy-1-O-[[[[[(2Z)-3,7-dimethylocta-2,6-dien-1-yl]oxy](hydroxy)phosphoryl]oxy](hydroxy)phosphoryl]-alpha-D-glucopyranose
0.04 - 1.97
2-(acetylamino)-2-deoxy-1-O-(hydroxy[[hydroxy(tridecyloxy)phosphoryl]oxy]phosphoryl)-alpha-D-glucopyranose
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apparent Kcat at 600 microM UDP-ManNAc
9.183
2-(acetylamino)-2-deoxy-1-O-(hydroxy[[hydroxy(tridecyloxy)phosphoryl]oxy]phosphoryl)-alpha-D-glucopyranose
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apparent Kcat at 600 microM UDP-ManNAc
0.52
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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apparent Kcat at 600 microM UDP-ManNAc
13.92
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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apparent Kcat at 600 microM UDP-ManNAc
0.04 - 1.97
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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apparent Kcat at 600 microM UDP-ManNAc
8.617
2-(acetylamino)-2-deoxy-1-O-[hydroxy[(hydroxy[[(2Z,6Z,10Z)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]oxy]phosphoryl)oxy]phosphoryl]-alpha-D-glucopyranose
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apparent Kcat at 600 microM UDP-ManNAc
0.866
2-(acetylamino)-2-deoxy-1-O-[[[[[(2Z)-3,7-dimethylocta-2,6-dien-1-yl]oxy](hydroxy)phosphoryl]oxy](hydroxy)phosphoryl]-alpha-D-glucopyranose
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apparent Kcat at 600 microM UDP-ManNAc
6.08
2-(acetylamino)-2-deoxy-1-O-[[[[[(2Z)-3,7-dimethylocta-2,6-dien-1-yl]oxy](hydroxy)phosphoryl]oxy](hydroxy)phosphoryl]-alpha-D-glucopyranose
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apparent Kcat at 600 microM UDP-ManNAc
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7.8
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TagA shows optimal acitvity at pH 7.8 in Bis-Tris propane buffer. Tested at room temperatur
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6 - 9
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40% of maximal activity at pH 6, 45% of maximal activity at pH 9
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AHU 1035
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brenda
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brenda
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AHU 1035
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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metabolism
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N-acetylmannosamine transferase catalyzes the first committed step of teichoic acid assembly
metabolism
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N-acetylmannosamine transferase catalyzes the first committed step of teichoic acid assembly
physiological function
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the tarA gene is dispensable for in vitro growth
physiological function
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the tagA gene is dispensable for in vitro growth
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glycerol, 25%, stabilizes
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Nonidet P-40, 0.1-0.2%, stabilizes
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-18°C, several months, no significant loss of activity
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Murazumi, N.; Kumita, K.; Araki, Y.; Ito, E.
Partial purification and properties of UDP-N-acetylmannosamine:N-acetylglucosaminyl pyrophosphorylundecaprenol N-acetylmannosaminyltransferase from Bacillus subtilis
J. Biochem.
104
980-984
1988
Bacillus subtilis, Bacillus subtilis AHU 1035
brenda
Zhang, Y.; Ginsberg, C.; Yuan, Y.; Walker, S.
Acceptor substrate selectivity and kinetic mechanism of Bacillus subtilis TagA
Biochemistry
45
10895-10904
2006
Bacillus subtilis
brenda
DElia, M.A.; Henderson, J.A.; Beveridge, T.J.; Heinrichs, D.E.; Brown, E.D.
The N-acetylmannosamine transferase catalyzes the first committed step of teichoic acid assembly in Bacillus subtilis and Staphylococcus aureus
J. Bacteriol.
191
4030-4034
2009
Bacillus subtilis, Staphylococcus aureus
brenda
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