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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
cyanohydrin glucosyltransferase, glucosyltransferase, uridine diphosphoglucose-cyanohydrin, UDPglucose:4-hydroxymandelonitrile glucosyltransferase,
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cyanohydrin glucosyltransferase
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glucosyltransferase, uridine diphosphoglucose-cyanohydrin
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UDPglucose:4-hydroxymandelonitrile glucosyltransferase
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UDP-glucose + 4-hydroxymandelonitrile = UDP + taxiphyllin
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hexosyl group transfer
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UDP-glucose:4-hydroxymandelonitrile glucosyltransferase
3,4-Dihydroxymandelonitrile can also act as acceptor.
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UDP-glucose + (R,S)-mandelonitrile
UDP + (R)-prunasin
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UDP-glucose + 3,4-dihydroxymandelonitrile
UDP + 3,4-dihydroxymandelonitrile glucoside
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UDP-glucose + 4-hydroxymandelonitrile
UDP + taxiphyllin
UDP-glucose + benzoic acid
UDP + benzoyl-beta-D-glucoside
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UDP-glucose + benzyl alcohol
UDP + benzyl-beta-D-glucoside
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UDP-glucose + mandelamide
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UDP-glucose + mandelic acid
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UDP-glucose + 4-hydroxymandelonitrile
UDP + taxiphyllin
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UDP-glucose + 4-hydroxymandelonitrile
UDP + taxiphyllin
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last step in biosynthesis of cyanogenic glucosides
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UDP-glucose + (R,S)-mandelonitrile
UDP + (R)-prunasin
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UDP-glucose + 4-hydroxymandelonitrile
UDP + taxiphyllin
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last step in biosynthesis of cyanogenic glucosides
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High salt concentrations
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160% activity observed at 0.6 M concentration of NaCl, KCl or ammonium sulfate with 4-hydroxymandelonitrile, and 20% activity observed at 0.1-0.2 M concentration with 3,4-dihydroxymandelonitrile
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0.32
UDP-glucose
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assay at pH 7.3 in the presence of 20 mM of (R,S)-mandelonitrile. The enzyme exclusively utilizes the (R)-enantiomer
0.5
UDP-glucose
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4-hydroxymandelonitrile or 3,4-dihydroxymandelonitrile
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0.012
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S)-mandelonitrile
0.31
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3,4-dihydroxymandelonitrile
0.94
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4-hydroxymandelonitrile
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7 - 8
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highest rates are realized in Tris-HCl buffer at pH 7.5
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5.8 - 9.3
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about 50% of activity maximum
6 - 9.5
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about 50% of activity maximum at pH 6 and pH 9.5
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wild black cherry
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brenda
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brenda
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brenda
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brenda
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47000
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1 * 47000, SDS-PAGE
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monomer
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1 * 47000, SDS-PAGE
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7.5
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unstable below, crude enzyme extract
587060
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-20°C, 10% glycerol, 72% of 2 days crude extract stable for several weeks
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-20°C, 10% glycerol, crude extract stable for several weeks
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method that includes chromatography on Sephadex G-25
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method that includes chromatography on Sephadex PD10 and DEAE-Sephadex
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Hösel, W.; Schiel, O.
Biosynthesis of cyanogenic glucosides: in vitro analysis of the glucosylation step
Arch. Biochem. Biophys.
229
177-186
1984
Triglochin maritima
brenda
Poulton, J.E.; Shin, S.I.
Prunasin biosynthesis by cell-free extracts from black cherry (Prunus serotina Ehrh.) fruits and leaves
Z. Naturforsch. C
38
369-374
1983
Prunus serotina
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brenda
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2.4.1.178 hydroxymandelonitrile glucosyltransferase
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