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Information on EC 2.4.1.173 - sterol 3beta-glucosyltransferase
for references in articles please use BRENDA:EC2.4.1.173
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.173 sterol 3beta-glucosyltransferase
IUBMB Comments
Not identical with EC 2.4.1.192 (nuatigenin 3β-glucosyltransferase) or EC 2.4.1.193 (sarsapogenin 3β-glucosyltransferase).
The enzyme appears in viruses and cellular organisms
- 2.4.1.173
- glucoside
-
steryl
-
glucosylation
-
pexophagy
- somnifera
-
withania
-
glucosyltransferases
-
withanolide
- stigmasterol
- synthesis
- sitosterol
-
3beta-hydroxy
- orbiculare
-
udp-glucose-dependent
- udpase
-
withaferin
showSynonyms
sterol glucosyltransferase, atg26, ugt80b1, ugt80a2, sgtl1, ugt51, udpg-sgtase, sterol glycosyltransferase, sgtl2, udp-glucose:sterol glucosyltransferase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Atg26
autophagy-related protein 26
ScAtg26
multidomain protein which possesses pleckstrin homology, GRAM (glucosyltransferase, Rab-like GTPase activators, and myotubularins), and catalytic UDP-glucosyl transferase, domains
SGT
SGT1
SGT2
SGTL1
sterol glucosyltransferase
-
-
-
-
sterol glucosyltransferase Ugt51/Paz4
sterol glycosyltransferase
sterol-beta-D-glucosyltransferase
-
-
-
-
sterol:UDPG glucosyltransferase
-
-
-
-
UDP-Glc:sterol glucosyltransferase 80B1
UDP-glucose sterol glucosyltransferase
UDP-glucose sterol glycosyltransferase
-
UDP-glucose-sterol beta-glucosyltransferase
-
-
-
-
UDP-glucose-sterol glucosyltransferase
-
-
-
-
UDP-glucose:sterol glucosyltransferase
UDP-glycosyltransferase 51
UDPG-SGTase
UDPG:sterol glucosyltransferase
-
-
-
-
UDPglucose:sterol glucosyltransferase
-
-
-
-
Ugt51
Ugt51/Paz4
UGT713B1
UGT80A2
UGT80B1
uridine diphosphoglucose-poriferasterol glucosyltransferase
-
-
-
-
uridine diphosphoglucose-sterol glucosyltransferase
-
-
-
-
SGTL1
gene encoding a member of the sterol glucosyltransferase in Withania somnifera
UDP-glucose:sterol glucosyltransferase
-
UDPG-SGTase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
cardenolide glucosides biosynthesis, solasodine glycosylation
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:sterol 3-O-beta-D-glucosyltransferase
Not identical with EC 2.4.1.192 (nuatigenin 3beta-glucosyltransferase) or EC 2.4.1.193 (sarsapogenin 3beta-glucosyltransferase).
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CAS REGISTRY NUMBER
COMMENTARY
123940-38-5
-
9075-00-7
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(22S,25S)-5alpha-solanidanin-3beta-ol + UDP-glucose
?
-
Substrates: demissidine 1.4% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
(22S,25S)-epoxy-furost-5-en-3beta,26-diol + UDP-glucose
?
-
Substrates: nuatigenin, 16.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
(25R)-5alpha-spirostan-3beta-ol + UDP-glucose
?
-
Substrates: tigogenin, 2.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
(25R)-5alpha-spirostan-3beta-ol-12-one + UDP-glucose
?
-
Substrates: hecogenin, 6.3% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
(25R)-spirost-5-en-3beta,25beta-diol + UDP-glucose
?
-
Substrates: isonuatigenin, 13.4% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
(25R)-spirost-5-en-3beta-ol + UDP-glucose
?
-
Substrates: diosgenin, 3.5% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
(25S)-5alpha,22betaN-spirosolanin-3beta-ol + UDP-glucose
?
-
Substrates: tomatidine, 2.8% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
24-methylene cholesterol + UDP-glucose
UDP + 24-methylene-cholesterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
3beta-hydroxy-16,17alpha-epoxypregnenolone + UDP-alpha-D-glucose
3beta-hydroxy-16,17alpha-epoxypregnenolone 3-beta-D-glucoside + UDP
-
Substrates: -
Products: -
Products: -
?
3beta-hydroxy-pregna-5,16-dien-20-one + UDP-glucose
UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
5alpha-cholest-7-en-3beta-ol + UDP-glucose
?
-
Substrates: 14.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
5alpha-cholestan-3beta-ol + UDP-glucose
?
-
Substrates: cholestanol, 12.0% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
5alpha-cholestan-3beta-ol + UDP-glucose
UDP + 3beta,5alpha-cholestan 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
androst-5-en-3beta-ol-17-one + UDP-glucose
?
-
Substrates: androstenolon, 16.0% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
brassicasterol + UDP-glucose
UDP + brassicasterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
cholest-5-en-22-on-3beta-ol + UDP-glucose
?
-
Substrates: 22-oxycholesterol, 133.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
cholest-5-en-3beta,19-diol + UDP-glucose
?
-
Substrates: 9.2% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
cholest-5-en-3beta,20alpha-diol + UDP-glucose
?
-
Substrates: 90.8% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
cholest-5-en-3beta,25-diol + UDP-glucose
?
-
Substrates: 25-hydroxycholesterol, 89.4% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
cholest-5-en-3beta-ol + UDP-glucose
?
-
Substrates: cholesterol, 98.5% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
cholest-5-en-3beta-ol-7-one + UDP-glucose
?
-
Substrates: 5.6% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
diosgenin + UDP-glucose
UDP + diosgenin 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
dUDP-glucose + iosgenin-3-O-glucoside
UDP + diosgenin-3-O-glucosyl-(1->6)-glucoside
-
Substrates: -
Products: -
Products: -
?
ergosterol + UDP-glucose
UDP + ergosterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
pregn-5-en-3beta-ol-20-one + UDP-glucose
?
-
Substrates: pregnenolon, 33.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
pregnenolene + UDP-glucose
UDP + pregnenolone 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
pregnenolone + UDP-glucose
UDP + pregnenolone 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
solasodine + UDP-glucose
UDP + solasodine 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
stigmasta-5,22-dien-3beta-ol + UDP-glucose
?
-
Substrates: stigmasterol, 49.2% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
stigmasta-5,24(28)-dien-3beta-ol + UDP-glucose
?
-
Substrates: 82.4% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
stigmastan-3beta-ol + UDP-glucose
?
-
Substrates: 38.7% of the activity with sitosterol (stigmast-5-en-3beta-ol)
Products: -
Products: -
?
UDP-glucose + 20(S)-protopanaxadiol
UDP + ginsenoside Rh2
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + 3beta-hydroxy-16,17alpha-epoxypregnenolone
?
Substrates: -
Products: -
Products: -
?
UDP-glucose + 3beta-hydroxy-16,17alpha-epoxypregnenolone
UDP + 3beta-hydroxy-16,17alpha-epoxypregnenolone 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + 3beta-hydroxy-pregna-5,16-dien-one
UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + 4-demethyl-obtusifoliol
UDP + 4-demethyl-obtusifoliol 3-beta-glucoside
-
Substrates: 58% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + 5-alpha-cholestanol
UDP + 5-alpha-cholestanol 3-beta-D-glucoside
-
Substrates: 8% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + 5-cholestenol
UDP + 5-cholestenol 3-beta-D-glucoside
-
Substrates: 12% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + 7-dehydrocholesterol
UDP + 7-dehydrocholesterol 3-beta-D-glucoside
-
Substrates: 6% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + alpha-spinasterol
UDP + alpha-spinasterol 3-beta-D-glucoside
-
Substrates: 31% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + dehydroepiandrosterone
UDP + dehydroepiandrosterone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + diosgenin-3-O-rhamnosyl-(1->2)-glucoside
UDP + diosgenin-3-O-rhamnosyl-(1->2)-[glucosyl-(1->6)]-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + epiandrosterone
UDP + epiandrosterone 3-beta-glucoside
-
Substrates: best substrate
Products: -
Products: -
?
UDP-glucose + ginsenoside Rh2
UDP + 20(S)-protopanaxadiol-3-O-glucosyl-(1->6)-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + isofucosterol
UDP + isofucosterol 3-beta-glucoside
-
Substrates: 73% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + pennogenin sapogenin
UDP + pennogenin 3-O-beta-D-glucopyranoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + pennogenin-3-O-rhamnosyl-(1->2)-glucoside
UDP + pennogenin-3-O-rhamnosyl-(1->2)-[glucosyl-(1->6)]-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + poriferasterol
UDP + poriferasterol 3-beta-glucoside
-
Substrates: synthesis of poriferasterol monoglucoside may be involved in differentiation
Products: -
Products: -
?
UDP-glucose + pregnenolone
UDP + pregnenolone 3-beta-glucoside
-
Substrates: 57% of the activity with epiandrosterone
Products: -
Products: -
?
UDP-glucose + ruscogenin
UDP + ruscogenin-3-O-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + ruscogenin-3-O-glucoside
UDP + ruscogenin-3-O-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + ruscogenin-3-O-glucoside
UDP + ruscogenin-3-O-glucosyl-(1->6)-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + solasodine
UDP + solasodine 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + withaferin A
UDP + ?
Substrates: -
Products: -
Products: -
?
UDP-glucose + withanolide A
UDP + ?
Substrates: -
Products: -
Products: -
?
3beta-hydroxy-pregna-5,16-dien-20-one + UDP-glucose
UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
3beta-hydroxy-pregna-5,16-dien-20-one + UDP-glucose
UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
beta-sitosterol + UDP-glucose
UDP + beta-sitosterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
beta-sitosterol + UDP-glucose
UDP + beta-sitosterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
cholesterol + UDP-glucose
UDP + cholesterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
cholesterol + UDP-glucose
UDP + cholesterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
dehydroepiandrosterone + UDP-glucose
UDP + dehydroepiandrosterone 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
dehydroepiandrosterone + UDP-glucose
UDP + dehydroepiandrosterone 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
stigmasterol + UDP-glucose
UDP + stigmasterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
stigmasterol + UDP-glucose
UDP + stigmasterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Arabidopsis thaliana Ws-0
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + beta-sitosterol
UDP + beta-sitosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + beta-sitosterol
UDP + beta-sitosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + beta-sitosterol
UDP + beta-sitosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + brassicasterol
UDP + brassicasterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + brassicasterol
UDP + brassicasterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
Substrates: 80% of the activity with poriferasterol
Products: -
Products: -
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
Substrates: 28% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
Substrates: -
Products: 4% of the products
Products: 4% of the products
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
Substrates: best substrate
Products: -
Products: -
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
Substrates: 68% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: cloned enzyme exhibits equal activity to the purified protein
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
Substrates: with sitosterol, best substrates
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates cholesterol
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: cloned enzyme exhibits equal activity to the purified protein
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: cloned enzyme exhibits equal activity to the purified protein
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates cholesterol
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
Substrates: 23% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
Substrates: 12% of the activity with poriferasterol
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates cholesterol
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates cholesterol
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
Substrates: best substrate
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates cholesterol
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates cholesterol
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates cholesterol
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates ergosterol
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
Substrates: 69% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates ergosterol
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
Substrates: 11% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
Substrates: 9% of the activity with poriferasterol
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates ergosterol
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates ergosterol
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates ergosterol
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates ergosterol
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates ergosterol
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + pregnenolone
UDP + pregnenolone 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + pregnenolone
UDP + pregnenolone 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + protopanaxadiol
UDP + protopanaxadiol 3-beta-D-glucoside
Substrates: an unnatural substrate of enzyme mutant 7_1
Products: -
Products: -
?
UDP-glucose + protopanaxadiol
UDP + protopanaxadiol 3-beta-D-glucoside
Substrates: an unnatural substrate of enzyme mutant 7_1
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates sitosterol
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: with cholesterol, best substrates
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates sitosterol
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: best substrate
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: maximal activity, the same as poriferasterol
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: greater activity than with other sterols
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates sitosterol
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates sitosterol
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: 88% of the activity with epiandrosterone
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: -
Products: 75% of the product
Products: 75% of the product
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates sitosterol
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates sitosterol
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates sitosterol
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: best substrate
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates stigmasterol
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
-
Substrates: 63% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates stigmasterol
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
-
Substrates: 14% of the activity with poriferasterol
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
-
Substrates: 50% of the activity with sitosterol
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates stigmasterol
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates stigmasterol
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
-
Substrates: 122% of the activity with epiandrosterone, highest specificity
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
-
Substrates: -
Products: 6% of the product
Products: 6% of the product
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates stigmasterol
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates stigmasterol
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: cloned enzyme glucosylates stigmasterol
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
Substrates: cloned enzyme glucosylates tomatidine
Products: -
Products: -
?
UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
Substrates: cloned enzyme glucosylates tomatidine
Products: -
Products: -
?
UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
Substrates: cloned enzyme glucosylates tomatidine
Products: -
Products: -
?
UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
Substrates: cloned enzyme glucosylates tomatidine
Products: -
Products: -
?
UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
Substrates: cloned enzyme glucosylates tomatidine
Products: -
Products: -
?
UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
Substrates: cloned enzyme glucosylates tomatidine
Products: -
Products: -
?
UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
Substrates: cloned enzyme glucosylates tomatidine
Products: -
Products: -
?
UDP-glucose + transandrosterone
UDP + transandrosterone 3beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + transandrosterone
UDP + transandrosterone 3beta-D-glucoside
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme only shows activity with sterols having hydroxyl group at C-3 position. Utilises only UDP-glucose, UDP-galactose cannot serve as the sugar donor.
Products: -
Products: -
?
additional information
?
-
-
Substrates: yamogenin is not substrate of this enzyme
Products: -
Products: -
?
additional information
?
-
-
Substrates: UDP-glucose is an effective glucose donor but not so UDP-mannose, UDP-galactose or UMP-glucose
Products: -
Products: -
?
additional information
?
-
Substrates: UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
Products: -
Products: -
?
additional information
?
-
-
Substrates: DELTA5-sterols are glucosylated at higher rate than DELTA7-sterols
Products: -
Products: -
?
additional information
?
-
-
Substrates: glucose 1-phosphate, GDP-glucose and ADP-glucose are inactive substrates
Products: -
Products: -
?
additional information
?
-
-
Substrates: presence and localization of double bonds in the ring system exert a pronounced effect on the rate of glucosylation
Products: -
Products: -
?
additional information
?
-
-
Substrates: the presence of a DELTA22 double bond decreases affinity of the sterol for the enzyme
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme can use CDP-glucose and TDP-glucose at 6 times slower rate
Products: -
Products: -
?
additional information
?
-
-
Substrates: coprostanol and epicholestanol are not glucosylated
Products: -
Products: -
?
additional information
?
-
-
Substrates: sterols possessing an alkyl group at C-24 are better substrates than C27 sterols
Products: -
Products: -
?
additional information
?
-
-
Substrates: stanols and sterols with conjugated double bonds in ring B are poor substrates
Products: -
Products: -
?
additional information
?
-
-
Substrates: all 3-B-OH sterols containing a double bond in position 5 are glucosylated and a double bond at C24 reduces glucosylation
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is a fungal virulence factor
Products: -
Products: -
?
additional information
?
-
Substrates: UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
Products: -
Products: -
?
additional information
?
-
-
Substrates: UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
Products: -
Products: -
?
additional information
?
-
-
Substrates: almost no activity with androsterone, digitoxigenin, digitoxin and testosterone
Products: -
Products: -
?
additional information
?
-
-
Substrates: UDP-glucose is an effective glucose donor but not so ADP-glucose, CDP-glucose, TDP-glucose, GDP-glucose, UDP-galactose or UDP-mannose
Products: -
Products: -
?
additional information
?
-
-
Substrates: UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
Products: -
Products: -
?
additional information
?
-
Substrates: UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
Products: -
Products: -
?
additional information
?
-
Substrates: sterol glucosyltransferase Ugt51/Paz4 is essential for pexophagy (peroxisome degradation), but not for macroautophagy in the methylotrophic yeast. Deletion of the catalytic domain does not impair protein localization, but abolishes pexophagy, suggesting that SG synthesis is required for this process
Products: -
Products: -
?
additional information
?
-
-
Substrates: sterol glucosyltransferase Ugt51/Paz4 is essential for pexophagy (peroxisome degradation), but not for macroautophagy in the methylotrophic yeast. Deletion of the catalytic domain does not impair protein localization, but abolishes pexophagy, suggesting that SG synthesis is required for this process
Products: -
Products: -
?
additional information
?
-
-
Substrates: the UGT51 gene is required for pexophagy and other vacuole related processes
Products: -
Products: -
?
additional information
?
-
Substrates: sterol glucosyltransferase Ugt51/Paz4 is essential for pexophagy (peroxisome degradation), but not for macroautophagy in the methylotrophic yeast. Deletion of the catalytic domain does not impair protein localization, but abolishes pexophagy, suggesting that SG synthesis is required for this process
Products: -
Products: -
?
additional information
?
-
-
Substrates: UDP-glucose is an effective glucose donor, not so ADP-glucose, CDP-glucose or GDP-glucose
Products: -
Products: -
?
additional information
?
-
Substrates: UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
Products: -
Products: -
?
additional information
?
-
-
Substrates: UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
Products: -
Products: -
?
additional information
?
-
Substrates: experimental results suggest that the enzyme is not involved in the cytoplasm to vacuole targeting pathway, macroautophagy and pexophagy
Products: -
Products: -
?
additional information
?
-
-
Substrates: experimental results suggest that the enzyme is not involved in the cytoplasm to vacuole targeting pathway, macroautophagy and pexophagy
Products: -
Products: -
?
additional information
?
-
Substrates: substrate molecular docking and structure analysis of wild-type and mutant enzymes, sugar donor binding mechanism of UGT51, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: substrate molecular docking and structure analysis of wild-type and mutant enzymes, sugar donor binding mechanism of UGT51, overview
Products: -
Products: -
?
additional information
?
-
Substrates: substrate molecular docking and structure analysis of wild-type and mutant enzymes, sugar donor binding mechanism of UGT51, overview
Products: -
Products: -
?
additional information
?
-
-
Substrates: enzyme is absolute specific to UDP-glucose
Products: -
Products: -
?
additional information
?
-
-
Substrates: glucose 1-phosphate, GDP-glucose and ADP-glucose are inactive substrates
Products: -
Products: -
?
additional information
?
-
-
Substrates: all 3-B-OH sterols containing a double bond in position 5 are glucosylated and a double bond at C24 reduces glucosylation
Products: -
Products: -
?
additional information
?
-
Substrates: SGT activity assays are carried out with a plant sterol mixture consisting of approximately 13% brassicasterol, 26% campesterol, 7% stigmasterol, and 53% beta-sitosterol in ethanol, and UDP-glucose
Products: -
Products: -
?
additional information
?
-
Substrates: SGT activity assays are carried out with a plant sterol mixture consisting of approximately 13% brassicasterol, 26% campesterol, 7% stigmasterol, and 53% beta-sitosterol in ethanol, and UDP-glucose
Products: -
Products: -
?
additional information
?
-
Substrates: SGT activity assays are carried out with a plant sterol mixture consisting of approximately 13% brassicasterol, 26% campesterol, 7% stigmasterol, and 53% beta-sitosterol in ethanol, and UDP-glucose
Products: -
Products: -
?
additional information
?
-
Substrates: SGT activity assays are carried out with a plant sterol mixture consisting of approximately 13% brassicasterol, 26% campesterol, 7% stigmasterol, and 53% beta-sitosterol in ethanol, and UDP-glucose
Products: -
Products: -
?
additional information
?
-
-
Substrates: SGT activity assays are carried out with a plant sterol mixture consisting of approximately 13% brassicasterol, 26% campesterol, 7% stigmasterol, and 53% beta-sitosterol in ethanol, and UDP-glucose
Products: -
Products: -
?
additional information
?
-
-
Substrates: may participate in the regulating of free sterol concentration in the cell
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with solasodine (25R-22alphaN-spirosol-5-enin-3beta-ol), solanidine (22S,25S-solanid-5-enin-3beta-ol), sarsasapogenin (25S-5beta-spirostan-3beta-ol), yamogenin (25S-spirost-5-en-3beta-ol), thiocholesterol (cholest-5-en-3beta-tiol), 5beta-cholestan-3beta-ol (coprostanol), 5alpha-cholestan-3alpha-ol (epicholestanol), 5beta-cholestan-3alpha-ol (epicoprostanol), lanosterol (4,4,14-trimethyl-5alpha-cholest-8-en-3beta-ol), and beta-amyrin (olean-12-en-3beta-ol) do not serve as glucose acceptors
Products: -
Products: -
?
additional information
?
-
-
Substrates: UDP-galactose, TDP-glucose and CDP-glucose are also suitable as a sugar source for sterol glucosylation but with distinctly lower rates
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is highly specific to sterols and shows no activity with non steroidal substrates. Amongst the sterols, the enzyme is position specific and works only with C-3 hydroxyl sterols, but the extradiol C-3 hydroxyl group is not glucosylated. Sterols with A-B ring 5-en (e.g. deacetyl-16-DPA) are glucosylated more efficiently than those with 5alpha-H. For the cytosolic enzyme, the relative substrate specificity of the membrane-bound enzyme is different. The membrane enzyme is more specific towards phytosterols and brassicasterol. It shows no activity towards secondary metabolits and flavonoids and isoflavonoids.
Products: -
Products: -
?
additional information
?
-
Substrates: the enzyme only shows activity with sterols having hydroxyl group at C-3 position. SGTL1 is more active toward sterols without side chain. Utilises only UDP-glucose, UDP-galactose can not serve as the sugar donor.
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme only shows activity with sterols having hydroxyl group at C-3 position. SGTL1 is more active toward sterols without side chain. Utilises only UDP-glucose, UDP-galactose can not serve as the sugar donor.
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is specific for the 3beta-hydroxy position and has a catalytic specificity to glycosylate withanolide and sterols
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is specific for the 3beta-hydroxy position and has a catalytic specificity to glycosylate withanolide and sterols
Products: -
Products: -
?
additional information
?
-
-
Substrates: the UGT51 gene is required for utilization of decane, but not for pexophagy
Products: -
Products: -
?
additional information
?
-
-
Substrates: p-nitrophenol, estrone and 4-alpha,10-beta-dimethyl-trans-decal-3-beta-ol are not substrates
Products: -
Products: -
?
stigmast-5-en-3beta-ol + UDP-glucose
additional information
-
-
Substrates: sitosterol, 100% of the activity
Products: 3-O-beta-D-monoglucopyranoside of sitosterol and 3-O-beta-D-monogalactopyranoside of sitosterol identified as products
Products: 3-O-beta-D-monoglucopyranoside of sitosterol and 3-O-beta-D-monogalactopyranoside of sitosterol identified as products
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-glucose + beta-sitosterol
UDP + beta-sitosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + poriferasterol
UDP + poriferasterol 3-beta-glucoside
-
Substrates: synthesis of poriferasterol monoglucoside may be involved in differentiation
Products: -
Products: -
?
UDP-glucose + withaferin A
UDP + ?
Substrates: -
Products: -
Products: -
?
UDP-glucose + withanolide A
UDP + ?
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Arabidopsis thaliana Ws-0
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme is a fungal virulence factor
Products: -
Products: -
?
additional information
?
-
Substrates: sterol glucosyltransferase Ugt51/Paz4 is essential for pexophagy (peroxisome degradation), but not for macroautophagy in the methylotrophic yeast. Deletion of the catalytic domain does not impair protein localization, but abolishes pexophagy, suggesting that SG synthesis is required for this process
Products: -
Products: -
?
additional information
?
-
-
Substrates: sterol glucosyltransferase Ugt51/Paz4 is essential for pexophagy (peroxisome degradation), but not for macroautophagy in the methylotrophic yeast. Deletion of the catalytic domain does not impair protein localization, but abolishes pexophagy, suggesting that SG synthesis is required for this process
Products: -
Products: -
?
additional information
?
-
-
Substrates: the UGT51 gene is required for pexophagy and other vacuole related processes
Products: -
Products: -
?
additional information
?
-
Substrates: sterol glucosyltransferase Ugt51/Paz4 is essential for pexophagy (peroxisome degradation), but not for macroautophagy in the methylotrophic yeast. Deletion of the catalytic domain does not impair protein localization, but abolishes pexophagy, suggesting that SG synthesis is required for this process
Products: -
Products: -
?
additional information
?
-
Substrates: experimental results suggest that the enzyme is not involved in the cytoplasm to vacuole targeting pathway, macroautophagy and pexophagy
Products: -
Products: -
?
additional information
?
-
-
Substrates: experimental results suggest that the enzyme is not involved in the cytoplasm to vacuole targeting pathway, macroautophagy and pexophagy
Products: -
Products: -
?
additional information
?
-
-
Substrates: may participate in the regulating of free sterol concentration in the cell
Products: -
Products: -
?
additional information
?
-
-
Substrates: the UGT51 gene is required for utilization of decane, but not for pexophagy
Products: -
Products: -
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Hg2+
Mg2+
Mn2+
Zn2+
additional information
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
diethyldicarbonate
-
protected against inactivation upon pre-incubation with stigmast-5-en-3beta-ol or UDP-glucose
Hg2+
-
strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 0.5
iodoacetamide
-
strong and irreversible inhibition by thiol directed reagents like 4-chloromercuribenzoate, N-methylmaleimide and iodoacetamide suggest the requirement of free-SH groups for the catalytic activity
Ni2+
-
strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 1.2
p-mercuribenzenesulfonate
-
50% of irreversible inhibition at 0.5 mM, UDP and UDPG-glucose protect from inhibition of 10 mM
Co2+
-
strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 1.3
N-ethylmaleimide
-
protected against inactivation upon pre-incubation with stigmast-5-en-3beta-ol or UDP-glucose
N-ethylmaleimide
-
strong and irreversible inhibition by thiol directed reagents like 4-chloromercuribenzoate, N-methylmaleimide and iodoacetamide suggest the requirement of free-SH groups for the catalytic activity
p-chloromercuribenzoate
-
potent inhibitory effect at 0.1 mM indicating that free SH groups are essential for activity
p-chloromercuribenzoate
-
strong and irreversible inhibition by thiol directed reagents like 4-chloromercuribenzoate, N-methylmaleimide and iodoacetamide suggest the requirement of free-SH groups for the catalytic activity
Triton X-100
-
moderate inhibition but strong inhibition at low concentrations
Triton X-100
-
at higher levels (0.3%), Triton X-100 inactivats the enzyme, giving 10% of the original activity after 30 min pre-incubation
UDP
-
the inhibitory effect of UTP and GTP is less prominent as compared to UDP
Zn2+
-
strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 0.04
additional information
-
not inhibited by ADP, GDP or inorganic phosphorus
-
additional information
-
inhibited at high ionic strength, 50% inhibition at 0.52 M NaF or 0.29 M NaCl; inhibited by dicarboxylic amino acids-modifying reagent Woodwards Reagent K protected against inactivation upon pre-incubation with UDP-glucose but not with stigmast-5-en-3beta-ol; no effect with Mg2+, Ca2+, Mn2+, Tween20, Tween 40, Tween 60, Tween 80, Tyloxapol, sodium deoxycholate, EDTA, EGTA, and reagents modifying serine, arginine and tyrosine side chains
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KOH
-
treatment with KOH pH 10.4 increase activity about 65%, hydroxyl ions can be used in place of detergents to reveal latent enzymatic sites
p-chloromercuribenzoate
-
potent inhibitory effect indicating that free SH groups are essential for activity
additional information
-
stimulated by acylglycerols containing unsaturated fatty acid residues
-
lecithin
-
stimulation by cotton and soybean lecithin but not with egg lecithin, appears to increase Vmax with sitosterol and Km of UDP-glucose
phospholipase
-
treatment with phospholipases A, C and D lowers activity 21-29%, the effect of phospholipase A is completely reverse by phosphatidylethanolamine but not by phosphatidylcholine or phosphatidylserine, after phospholipase C or D treatment each phospholipid brings about a partial recovery of activity but phosphatidylethanolamine is superior
-
phospholipase
-
phospholipase A2, slight inhibition of latent activity but stimulates the patent activity, it can be used in place of detergents to reveal latent enzymatic sites
-
Phospholipids
-
phosphatidylcholine + phosphatidylglycerol or phosphatidylcholine + phosphatidic acid increase Vmax and Km with respect to phosphatidylcholine alone
Phospholipids
-
the highest Vmax and Km is observed for the ratios 3:1 of phosphatidylcholine + phosphatidylglycerol and 3:1 phosphatidylcholine + phosphatidic acid
Phospholipids
-
stimulation of activity by solubilization of enzyme still membrane-bound
Triton X-100
-
activity stimulated by 0.1-0.5% with highest stimulation at 0.25%
Triton X-100
-
activity stimulated by 0.1-1% with 6.5fold stimulation at 0.1%, highest activity stimulation with respect to others non-ionic detergents
Triton X-100
-
up to 0.03% Triton X-100 in the assay mixture, the catalytic activity is enhanced
Triton X-100
-
stimulation at low concentrations, at intermediate concentration 0.4-0.8% this stimulation is abolished, it appears to solubilize enzyme still membrane-bound
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Heart Failure
Empagliflozin Increases Cardiac Energy Production in Diabetes: Novel Translational Insights Into the Heart Failure Benefits of SGLT2 Inhibitors.
Infections
Expression of sodium-glucose co-transporter and brush border disaccharidases in Giardia lamblia infected rat intestine.
Melanoma, Experimental
Novel sterol glucosyltransferase in the animal tissue and cultured cells: Evidence that glucosylceramide as glucose donor.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.01
3beta-hydroxy-16,17alpha-epoxypregnenolone
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
0.01
3beta-hydroxy-pregna-5,16-dien-one
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
0.0084
pennogenin-3-O-rhamnosyl-(1->2)-glucoside
-
pH 8.0, 37°C
-
0.04
beta-sitosterol
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
0.0045
brassicasterol
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
0.04
dehydroepiandrosterone
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
0.004
ergosterol
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
0.006
pregnenolone
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
0.007
solasodine
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
0.027
stigmasterol
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
0.025
transandrosterone
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
0.008
UDP-glucose
activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.002
pennogenin-3-O-rhamnosyl-(1->2)-glucoside
-
pH 8.0, 37°C
-
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.5
Hg2+
Withania somnifera
-
strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 0.5
1.2
Ni2+
Withania somnifera
-
strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 1.2
1.3
Co2+
Withania somnifera
-
strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 1.3
0.04
Zn2+
Withania somnifera
-
strongly inhibits the sterol glucosyltransferase activity, IC50 (mM): 0.04
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
the cytosolic enzyme shows 100% activity with 24-methylene chloseterol as substrate. Using Dehydroepiandrosterone, Stigmasterol, Brassicasterol, beta-sitosterol or Deacetyl-16-DPA the activtity is 75% - 44%. Using Diosgenin, Solasdine or Pregnenolene the activity is 31,5% - 14.1%. Using Cholesterol, 20S,22R-1alpha, 3beta-dihydroxy-witha-5,24-dienolide, Hecogenin, Tigogenin, Quercetin or Daidzein the activity is 8.9% - 2.1%. There is no activity, when using Euphol, Ursolic acid, Testosterone, Estradiol or Single glucose withanolide as substrate. Sterol glucosyltransferase activity is monitored radiometricaly using UDP-[U-14C] glucose as the radioactive substrate in the radioactive substrate
additional information
-
the membrane bound enzyme shows 100% activity with 24-methylene chloseterol as substrate. Using Deacetyl-16-DPA, beta-sitosterol, Brassicasterol, Dehydroepiandrosterone, Pregnenolene, Stigmasterol or Cholesterol the activtity is 577% - 124%. The enzyme shows 2% activity with Diosgenin as substrate. It does not show any activity with Solasodine 16,17alpha-epoxy prednenolene, Hecogenin, 20S,22R-1alpha, 3beta-dihydroxy-witha-5,24-dienolide, Tigogenin, Quercetin, Daidzein, Euphol, Ursolic acid, Testosterone, Estradiol or Single glucose withanolide.
additional information
the activity obtained with dehydroepiandrosterone is 100%. 3-beta-hydroxy-pregna-5,16-dien-one, transandrosterone, 3-beta-hydroxy-16, 17-alpha-epoxy-pregnenolone, pregnenolone, stigmasterol, beta-sitosterol, ergosterol, brassicasterol and solasodine give activities with the enzyme SGTL1. 5-alpha-cholestan-3-beta-ol and cholesterol show no activity with the enzyme. Activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
additional information
-
the activity obtained with dehydroepiandrosterone is 100%. 3-beta-hydroxy-pregna-5,16-dien-one, transandrosterone, 3-beta-hydroxy-16, 17-alpha-epoxy-pregnenolone, pregnenolone, stigmasterol, beta-sitosterol, ergosterol, brassicasterol and solasodine give activities with the enzyme SGTL1. 5-alpha-cholestan-3-beta-ol and cholesterol show no activity with the enzyme. Activity is assayed radiometrically using UDP-[U-14C] glucose, at pH 8.0 and 37°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
7.8
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 9
-
the enzyme has an optimum pH for activity around 8.0 with half maximal activities at 7.0 and above 9.0
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
a decrease in the transcript level is observed within 15 min when the plants are shifted from 25 to 42°C
additional information
-
a decrease in the transcript level is observed within 15 min when the plants are shifted from 25 to 42°C
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mutant defective in the orthologue of UGT51, encoding sterol glucosyltransferase
-
-
brenda
mutant defective in the orthologue of UGT51, encoding sterol glucosyltransferase
-
-
brenda
the incubation of leaf discs in the presence of salicylic acid results in transient accumulation of SGTL1 transcript. It increases gradually to reach the maxima of 2.15fold at 12 h followed by a decline.
Swissprot
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
from 4 weeks old plants, expressend in significantly lower level than in leaves and roots
brenda
additional information
in small green, mature green, and mature red fruits. SlSGT1 mRNA levels decrease when fruits start to ripe (breaker and orange stages) but increase again in red ripe fruits
brenda
in small green, mature green, and mature red fruits. SlSGT2 mRNA levels decrease when fruits start to ripe (breaker and orange stages) but increase again in red ripe fruits
brenda
expression of SlSGT3 is clearly different from other isozymes since its mRNA levels are low in developing fruits (small green and mature green stages) but increase sharply when fruits start to ripe and remain at similar high levels until the red mature stage
brenda
-
crude extracts have 2-5 times lower activity than cell cultures
brenda
from 4 weeks old plants, young and mature, 2.21fold higher enzyme level than in the stem, SGTL1 transcript accumulates at a higher level in mature leaves (2.41fold) than in the young leaves and seedlings
brenda
from 4 weeks old plants, 3.13fold higher expression of the enzyme than in the stem
brenda
lower expression of the enzyme than in mature leaves
brenda
additional information
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT1 is the most intensely expressed in root, leaf, and flower tissues, being roots and leaves the tissues where the highest levels of SlSGT1 mRNA are detected. SlSGT1 is again the most actively expressed SlSGT gene in both developing (small green and mature green stages) and mature red fruits
brenda
additional information
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT1 is the most intensely expressed in root, leaf, and flower tissues, being roots and leaves the tissues where the highest levels of SlSGT1 mRNA are detected. SlSGT1 is again the most actively expressed SlSGT gene in both developing (small green and mature green stages) and mature red fruits
brenda
additional information
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT1 is the most intensely expressed in root, leaf, and flower tissues, being roots and leaves the tissues where the highest levels of SlSGT1 mRNA are detected. SlSGT1 is again the most actively expressed SlSGT gene in both developing (small green and mature green stages) and mature red fruits
brenda
additional information
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT1 is the most intensely expressed in root, leaf, and flower tissues, being roots and leaves the tissues where the highest levels of SlSGT1 mRNA are detected. SlSGT1 is again the most actively expressed SlSGT gene in both developing (small green and mature green stages) and mature red fruits
brenda
additional information
-
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT1 is the most intensely expressed in root, leaf, and flower tissues, being roots and leaves the tissues where the highest levels of SlSGT1 mRNA are detected. SlSGT1 is again the most actively expressed SlSGT gene in both developing (small green and mature green stages) and mature red fruits
brenda
additional information
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening
brenda
additional information
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening
brenda
additional information
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening
brenda
additional information
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening
brenda
additional information
-
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening
brenda
additional information
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT4 transcripts are barely detectable in all samples analyzed.
brenda
additional information
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT4 transcripts are barely detectable in all samples analyzed.
brenda
additional information
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT4 transcripts are barely detectable in all samples analyzed.
brenda
additional information
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT4 transcripts are barely detectable in all samples analyzed.
brenda
additional information
-
the SlSGT isozyme encoding genes have specialized but still largely overlapping expression patterns in different organs of tomato plants and throughout the different stages of fruit development and ripening. SlSGT4 transcripts are barely detectable in all samples analyzed.
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
during micropexophagy, the glucosyltransferase is associated with the micropexophagic apparatus. GRAM domain is essential for proper protein association with its target membrane
brenda
-
during micropexophagy, the glucosyltransferase is associated with the micropexophagic apparatus. GRAM domain is essential for proper protein association with its target membrane
-
brenda
during micropexophagy, the glucosyltransferase is associated with the micropexophagic apparatus. GRAM domain is essential for proper protein association with its target membrane
-
brenda
-
during micropexophagy, the glucosyltransferase is associated with the micropexophagic apparatus. GRAM domain is essential for proper protein association with its target membrane
-
-
brenda
the major difference between the two genes in the plant is the presence of the transmembrane domain in isozyme GhSGT2
brenda
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the major difference between the two genes in the plant is the presence of the transmembrane domain in isozyme GhSGT2
-
brenda
additional information
UGT80B1:GFP is localized in the hechtian strands, connecting the retracting protoplast from the cell wall. Wild-type exhibits clear cell periphery localization of SCM:GFP in one of the two cell files, differentiating developing H cells from N cells. In ugt80B1 mutants, a decrease in cell peripheral localization of SCM:GFP can be seen, and signal is detected in both H and N cell files indicating a loss in cell-type specific accumulation of SCRAMBLED (SCM). An increase in cytoplasmic punctae labeled with SCM:GFP is visible in both cell files in ugt80B1
-
brenda
additional information
-
UGT80B1:GFP is localized in the hechtian strands, connecting the retracting protoplast from the cell wall. Wild-type exhibits clear cell periphery localization of SCM:GFP in one of the two cell files, differentiating developing H cells from N cells. In ugt80B1 mutants, a decrease in cell peripheral localization of SCM:GFP can be seen, and signal is detected in both H and N cell files indicating a loss in cell-type specific accumulation of SCRAMBLED (SCM). An increase in cytoplasmic punctae labeled with SCM:GFP is visible in both cell files in ugt80B1
-
-
brenda
additional information
the major difference between the two genes in the plant is the presence of the transmembrane domain in isozyme GhSGT2
-
brenda
additional information
the major difference between the two genes in the plant is the presence of the transmembrane domain in isozyme GhSGT2
-
brenda
additional information
-
the major difference between the two genes in the plant is the presence of the transmembrane domain in isozyme GhSGT2
-
brenda
additional information
-
the major difference between the two genes in the plant is the presence of the transmembrane domain in isozyme GhSGT2
-
-
brenda
additional information
SGT isozymes from Solanum lycopersicum are located in cytosol and/or plasma membrane, overview
-
brenda
additional information
SGT isozymes from Solanum lycopersicum are located in cytosol and/or plasma membrane, overview
-
brenda
additional information
SGT isozymes from Solanum lycopersicum are located in cytosol and/or plasma membrane, overview
-
brenda
additional information
SGT isozymes from Solanum lycopersicum are located in cytosol and/or plasma membrane, overview
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brenda
additional information
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SGT isozymes from Solanum lycopersicum are located in cytosol and/or plasma membrane, overview
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brenda
Highest Expressing Human Cell Lines
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
evolution
phylogenetic tree of UGT80- and UGT713-related proteins, overview
evolution
UGT80 proteins belong to a third family that is hypothesized to be involved in sterol glucoside synthesis, phylogenetic tree of UGT80- and UGT713-related proteins, overview
evolution
enzyme UGT51 is a member of the GT1 family, GT-B fold group, comprising two Rossman-like domains
evolution
the enzyme belongs to the UDP-glucose sterol glycosyltransferases family of developmental and stress regulated genes that encode cytosolic and membrane-associated forms of the enzyme. Identification and functional characterization of the four members, SlSGT1-4, of the tomato cv. Micro-Tom
evolution
the genome of Arabidopsis thaliana contains two genes coding for UDP-Glc:sterol-glucosyltransferases, UGT80A2 and UGT80B1, and studies of mutant lines indicate that they are only partially redundant
evolution
-
phylogenetic tree of UGT80- and UGT713-related proteins, overview
-
evolution
-
UGT80 proteins belong to a third family that is hypothesized to be involved in sterol glucoside synthesis, phylogenetic tree of UGT80- and UGT713-related proteins, overview
-
evolution
-
the genome of Arabidopsis thaliana contains two genes coding for UDP-Glc:sterol-glucosyltransferases, UGT80A2 and UGT80B1, and studies of mutant lines indicate that they are only partially redundant
-
evolution
-
enzyme UGT51 is a member of the GT1 family, GT-B fold group, comprising two Rossman-like domains
-
malfunction
-
the atg26 mutant is defective in Appressorium-mediated host invasion
malfunction
ugt80B1 mutants displaya a significant reduction only in campesteryl, brassicasteryl, and cholesteryl glucosides, but not sitosteryl or stigmasteryl glucosides
malfunction
analysis of the function of SGTs by silencing SGTL1, SGTL2 and SGTL4 in Withania somnifera. Downregulation of SGTs by artificial miRNAs leads to the enhanced accumulation of withanolide A, withaferin A, sitosterol, stigmasterol and decreased content of withanoside V in virus induced gene silencing (VIGS) lines. This is further correlated with increased expression of WsHMGR, WsDXR, WsFPPS, WsCYP710A1, WsSTE1 and WsDWF5 genes, involved in withanolide biosynthesis. These variations of withanolide concentrations in silenced lines result in pathogen susceptibility as compared to control plants. The infection of Alternaria alternata causes increased salicylic acid, callose deposition, superoxide dismutase and H2O2 in aMIR-VIGS lines. The expression of biotic stress related genes, namely, WsPR1, WsDFS, WsSPI and WsPR10 is also enhanced in aMIR-VIGS lines in time dependent manner. Salicylic acid level increases the expression of defence related genes in silenced lines. A positive feedback regulation of withanolide biosynthesis occurs by silencing of SGTLs which results in reduced biotic tolerance
malfunction
inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis thaliana resistance to Botrytis cinerea infection, which correlates with increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. Analysis of the response to necrotrophic fungus Botrytis cinerea in an Arabidopsis thaliana mutant that is severely impaired in steryl glycosides biosynthesis due to the inactivation of the two sterol glucosyltransferases, UGT80A2 and UGT80B1. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. After fungus infection, the expression of genes involved in the indole glucosinolate biosynthesis is also upregulated at a higher degree in the ugt80A2;B1 mutant than in wild-type plants
malfunction
the plasma membrane cell fate regulator, SCRAMBLED (SCM), is mislocalized in ugt80B1 mutants, underscoring the aberrant root epidermal cell patterning. GFP-tagged SCM is localized to the cytoplasm in a non cell type dependent manner instead of the hair (H) cell plasma membrane in these mutants. Abnormal root hair cell patterning in ugt80B1 mutants is likely the direct result of expression of GL2 in H cell files in this mutant. The aberrant expression of GL2 is caused by the mislocalization of SCM away from the cell periphery, reducing the capacity of the receptor to mediate positional information to the cell. Reductions in specific sterol glucosides might be responsible for the disruption of cell fate regulators in these ugt80B1 mutants. The mislocalization of SCM to the cytoplasm can point to a role for sterol glucosides in vesicular trafficking or plasma membrane protein targeting. Deficiencies in specific sterol glucosides are sufficient to disrupt normal cell function and point to a possible role for sterol glucosides in cargo transport and/or protein targeting to the plasma membrane. Aberrant subcellular localization of SCM:GFP in ugt80B1 epidermal cells from the elongation zone of the root
malfunction
-
down-regulation of TfS3GT2 in fenugreek generally leads to reduced levels of diosgenin or yamogenin-derived steroidal saponins
malfunction
down-regulation of tomato sterol glycosyltransferase 1 perturbs plant development and facilitates viroid infection
malfunction
-
ugt80B1 mutants displaya a significant reduction only in campesteryl, brassicasteryl, and cholesteryl glucosides, but not sitosteryl or stigmasteryl glucosides
-
malfunction
-
the plasma membrane cell fate regulator, SCRAMBLED (SCM), is mislocalized in ugt80B1 mutants, underscoring the aberrant root epidermal cell patterning. GFP-tagged SCM is localized to the cytoplasm in a non cell type dependent manner instead of the hair (H) cell plasma membrane in these mutants. Abnormal root hair cell patterning in ugt80B1 mutants is likely the direct result of expression of GL2 in H cell files in this mutant. The aberrant expression of GL2 is caused by the mislocalization of SCM away from the cell periphery, reducing the capacity of the receptor to mediate positional information to the cell. Reductions in specific sterol glucosides might be responsible for the disruption of cell fate regulators in these ugt80B1 mutants. The mislocalization of SCM to the cytoplasm can point to a role for sterol glucosides in vesicular trafficking or plasma membrane protein targeting. Deficiencies in specific sterol glucosides are sufficient to disrupt normal cell function and point to a possible role for sterol glucosides in cargo transport and/or protein targeting to the plasma membrane. Aberrant subcellular localization of SCM:GFP in ugt80B1 epidermal cells from the elongation zone of the root
-
malfunction
Arabidopsis thaliana Ws-0
-
inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis thaliana resistance to Botrytis cinerea infection, which correlates with increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. Analysis of the response to necrotrophic fungus Botrytis cinerea in an Arabidopsis thaliana mutant that is severely impaired in steryl glycosides biosynthesis due to the inactivation of the two sterol glucosyltransferases, UGT80A2 and UGT80B1. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. After fungus infection, the expression of genes involved in the indole glucosinolate biosynthesis is also upregulated at a higher degree in the ugt80A2;B1 mutant than in wild-type plants
-
metabolism
UGT51 is not involved in autophagy-related pathways in Saccharomyces cerevisiae
metabolism
crosstalk between hormone signaling pathways, particularly those mediated by salicylate and jasmonate, has been found to contribute to plant resistance to different types of pathogens. The results suggest that the salicylate-mediated defense pathway is not involved in the response of the ugt80A2;B1 mutant to Bortrytis cinerea infection. But camalexin and, probably, also indole glucosinolates are actively involved in the enhanced resistance of the ugt80A2;B1 mutant to Bortrytis cinerea infection. The synthesis of alkylglucosinolates in the ugt80A2;B1 mutant is not affected by Bortrytis cinerea infection
metabolism
-
the enzyme is involved in biosynthesis of steroidal saponins. Role in transferring a glucose group onto the C-3 hydroxyl group of diosgenin or yamogenin
metabolism
the enzyme is related to the biosynthesis of polyphyllin. It catalyses C-3 glycosylation to pennogenin sapogenin of polyphyllin, which is the first glycosylation step of the biosynthetic pathway of polyphyllins
metabolism
-
UGT51 is not involved in autophagy-related pathways in Saccharomyces cerevisiae
-
metabolism
Arabidopsis thaliana Ws-0
-
crosstalk between hormone signaling pathways, particularly those mediated by salicylate and jasmonate, has been found to contribute to plant resistance to different types of pathogens. The results suggest that the salicylate-mediated defense pathway is not involved in the response of the ugt80A2;B1 mutant to Bortrytis cinerea infection. But camalexin and, probably, also indole glucosinolates are actively involved in the enhanced resistance of the ugt80A2;B1 mutant to Bortrytis cinerea infection. The synthesis of alkylglucosinolates in the ugt80A2;B1 mutant is not affected by Bortrytis cinerea infection
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physiological function
-
required for full virulence of Colletotrichum orbiculare
physiological function
the membrane-bound enzyme, UDP-glucose:sterol glucosyltransferase, controls the steryl glycoside synthesis
physiological function
-
the enzyme is a cholesteryl glucoside synthetase, whose activity in lipid rafts might act as a potential factor in the thermal sensing reaction. It is involved in induction of heat shock protein factors Hsp70 and HSF-1 under heat stress
physiological function
no role in steryl glucoside synthesis for UGT713B1/At5g24750
physiological function
steryl glucoside may be specific only for cellulose biosynthesis in cotton fibers because mature fibers contain more than 95% of cellulose
physiological function
steryl glucoside may be specific only for cellulose biosynthesis in cotton fibers because mature fibers contain more than 95% of cellulose. Isozyme GhSGT2 mainly participates in catalyzing glucosylation of membrane sterols
physiological function
-
ectopic enzyme overexpression in Withania somnifera promotes growth, enhances glycowithanolide and provides tolerance to abiotic and biotic stresses. Glycosylation not only stabilizes the products but also alters their physiological activities and governs intracellular distribution
physiological function
UGT80A2 accounts for most of the sitosteryl and stigmasteryl glucoside production in seeds
physiological function
UGT80B1 plays a specialized role in steryl glucoside synthesis
physiological function
sterol glycosyltransferases (SGTs) catalyse transfer of glycon moiety to sterols and their related compounds to produce diverse glyco-conjugates or steryl glycosides with different biological and pharmacological activities. SGTs from Withania somnifera play a role in abiotic stresses
physiological function
sterol glycosyltransferases (SGTs) catalyze the formation of a variety of glycosylated sterol derivatives and are involved in producing a plethora of bioactive natural products. Sterol glycosyltransferases as members of UGTs are involved in transferring a sugar from UDP-sugar to various SGs metabolites, including hormones and secondary metabolites
physiological function
sterol glycosyltransferases (SGTs) catalyze the glycosylation of the free hydroxyl group at C-3 position of sterols to produce sterol glycosides. Solanum contain very high levels of glycosylated sterols, which in the case of tomato may account for more than 85% of the total sterol content. Tomato SGT isozymes show the ability to glycosylate different sterol species including cholesterol, brassicasterol, campesterol, stigmasterol, and beta-sitosterol, which is consistent with the occurrence in their primary structure of the putative steroid-binding domain found in steroid UDP-glucuronosyltransferases and the UDP-sugar binding domain characteristic for a superfamily of nucleoside diphosphosugar glycosyltransferases. The SlSGT isozyme are differentially regulated in response to biotic and abiotic stress conditions. Tomato SGT isozymes play overlapping but not completely redundant biological functions involved in mediating developmental and stress responses. Changes in the relative proportions of sterols alter membrane fluidity and permeability and hence regulate different membrane functions such as simple and carrier-mediated diffusion, active transport across the membrane, and the activity of membrane-associated proteins
physiological function
sterol glycosyltransferases (SGTs) catalyze the glycosylation of the free hydroxyl group at C-3 position of sterols to produce sterol glycosides. Solanum contain very high levels of glycosylated sterols, which in the case of tomato may account for more than 85% of the total sterol content. Tomato SGT isozymes show the ability to glycosylate different sterol species including cholesterol, brassicasterol, campesterol, stigmasterol, and beta-sitosterol, which is consistent with the occurrence in their primary structure of the putative steroid-binding domain found in steroid UDP-glucuronosyltransferases and the UDP-sugar binding domain characteristic for a superfamily of nucleoside diphosphosugar glycosyltransferases. The SlSGT isozyme are differentially regulated in response to biotic and abiotic stress conditions. Changes in the relative proportions of sterols alter membrane fluidity and permeability and hence regulate different membrane functions such as simple and carrier-mediated diffusion, active transport across the membrane, and the activity of membrane-associated proteins. Tomato SGT isozymes play overlapping but not completely redundant biological functions involved in mediating developmental and stress responses
physiological function
role of sterol glycosyltransferases in proper plant development
physiological function
-
no role in steryl glucoside synthesis for UGT713B1/At5g24750
-
physiological function
-
UGT80A2 accounts for most of the sitosteryl and stigmasteryl glucoside production in seeds
-
physiological function
-
UGT80B1 plays a specialized role in steryl glucoside synthesis
-
physiological function
-
sterol glycosyltransferases (SGTs) catalyze the formation of a variety of glycosylated sterol derivatives and are involved in producing a plethora of bioactive natural products. Sterol glycosyltransferases as members of UGTs are involved in transferring a sugar from UDP-sugar to various SGs metabolites, including hormones and secondary metabolites
-
physiological function
-
steryl glucoside may be specific only for cellulose biosynthesis in cotton fibers because mature fibers contain more than 95% of cellulose
-
physiological function
-
steryl glucoside may be specific only for cellulose biosynthesis in cotton fibers because mature fibers contain more than 95% of cellulose. Isozyme GhSGT2 mainly participates in catalyzing glucosylation of membrane sterols
-
physiological function
-
ectopic enzyme overexpression in Withania somnifera promotes growth, enhances glycowithanolide and provides tolerance to abiotic and biotic stresses. Glycosylation not only stabilizes the products but also alters their physiological activities and governs intracellular distribution
-
additional information
-
alteration of the membrane physical state caused by heat stress may be linked to activate sterol glucosyltransferase to form cholesteryl glucoside
additional information
a long hydrophobic cavity, 9.2 A in width and 17.6 A in length located at the N-terminal domain of UGT51, is suitable for the accommodation of sterol acceptor substrates. A short, conserved sequence of S847-M851 is identified at the bottom of the hydrophobic cavity, which might be the steroid binding site and play an important role for the UGT51 catalytic specificity towards sterols. Molecular docking simulations revealing the sugar acceptor specificity, overview. The N- and C-terminal domains predominantly dictate acceptor and donor specificities, respectively. The donor substrate binds in a deep inter-domain pocket and a hydrophobic crevice on the surface of the N-terminal domain, which is proposed to be the binding site of the aglycone substrate
additional information
-
a long hydrophobic cavity, 9.2 A in width and 17.6 A in length located at the N-terminal domain of UGT51, is suitable for the accommodation of sterol acceptor substrates. A short, conserved sequence of S847-M851 is identified at the bottom of the hydrophobic cavity, which might be the steroid binding site and play an important role for the UGT51 catalytic specificity towards sterols. Molecular docking simulations revealing the sugar acceptor specificity, overview. The N- and C-terminal domains predominantly dictate acceptor and donor specificities, respectively. The donor substrate binds in a deep inter-domain pocket and a hydrophobic crevice on the surface of the N-terminal domain, which is proposed to be the binding site of the aglycone substrate
additional information
sterol glucosides patterns of wild-type and mutant roots, overview. The ugt80B1 mutant shows a significant reduction in stigmasteryl glucosides only
additional information
-
sterol glucosides patterns of wild-type and mutant roots, overview. The ugt80B1 mutant shows a significant reduction in stigmasteryl glucosides only
-
additional information
-
a long hydrophobic cavity, 9.2 A in width and 17.6 A in length located at the N-terminal domain of UGT51, is suitable for the accommodation of sterol acceptor substrates. A short, conserved sequence of S847-M851 is identified at the bottom of the hydrophobic cavity, which might be the steroid binding site and play an important role for the UGT51 catalytic specificity towards sterols. Molecular docking simulations revealing the sugar acceptor specificity, overview. The N- and C-terminal domains predominantly dictate acceptor and donor specificities, respectively. The donor substrate binds in a deep inter-domain pocket and a hydrophobic crevice on the surface of the N-terminal domain, which is proposed to be the binding site of the aglycone substrate
-
Show AA Sequence and Transmembrane Helices (2892 entries)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
136000
56000
-
purification by Q-Sepharose fast flow column, Hitrap blue column and SDS-PAGE
66000
cloned, N-terminal peptide of 133 amino acid could be cleaved resulting in a molecular weight of 52000 in agreement with 56000 of the purified enzyme
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
homodimer
homodimer
UGT51 forms a dimer in solution. The dimeric structure of UGT51 has two subunits packed against each other in a parallel fashion and adopts a butterfly shape. The full length of UGT51 contain 1198 amino acids, which can be divided into several domains: pleckstrin homology (PH) domain, GRAM domain and catalytic domain. The PH domain responsible interact with membrane lipids, the GRAM domain is essential for proper protein association with its target membrane, while the catalytic domain is essentiell for synthesis steroid glycosides. Each subunit displays the typical GT-B fold of GTs, comprising two distinct N- and C-terminal Rossman-fold domains having a similar topology and connected by a linker peptide and a long C-terminal helix. The N-terminal domain, a seven-stranded parallel beta-sheet flanked by nine alpha-helices, and the C-terminal domain, a six-stranded beta-sheet surrounded by five alpha-helices, are assumed to bind the aglycone and the nucleotide-sugar donor, respectively. The C-terminal helices alpha7 (residues 1129-1144) and alpha8 (residues 1147-1171) cross and interact with each Rossman-fold domain
homodimer
-
UGT51 forms a dimer in solution. The dimeric structure of UGT51 has two subunits packed against each other in a parallel fashion and adopts a butterfly shape. The full length of UGT51 contain 1198 amino acids, which can be divided into several domains: pleckstrin homology (PH) domain, GRAM domain and catalytic domain. The PH domain responsible interact with membrane lipids, the GRAM domain is essential for proper protein association with its target membrane, while the catalytic domain is essentiell for synthesis steroid glycosides. Each subunit displays the typical GT-B fold of GTs, comprising two distinct N- and C-terminal Rossman-fold domains having a similar topology and connected by a linker peptide and a long C-terminal helix. The N-terminal domain, a seven-stranded parallel beta-sheet flanked by nine alpha-helices, and the C-terminal domain, a six-stranded beta-sheet surrounded by five alpha-helices, are assumed to bind the aglycone and the nucleotide-sugar donor, respectively. The C-terminal helices alpha7 (residues 1129-1144) and alpha8 (residues 1147-1171) cross and interact with each Rossman-fold domain
-
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant UGT51 alone and in complex with UDP-glucose, hanging drop vapor diffusion method, mixing of 20 mg/ml protein in 20 mM Tris-HCl, 500 mM NaCl, and 2 mM DTT, with reservoir solution containing 0.2 M ammonium acetate, 0.1 M HEPES, pH 7.5, 25% w/v PEG 3350 for the free enzyme, or containing 0.2 M MgCl2, 0.1 M Tris, pH 8.5, and 25% w/v PEG 3350 for the enzyme complex, receiving microcrystals, optimized crystallization protocol involves dispensing 0.0015 ml of 20 mg/ml protein and 0.001 ml of reservoir solution (containing 0.2 M MgCl2, 0.1 M Tris pH 8.5 and 25% w/v PEG 3350) and 500 nl of 0.001 diluted crystal seed stock solution, rod protein crystals appear after 3 days, 14°C, crystals are soaked in cryoprotectant solution with 5 mM UDP-glucose, X-ray diffraction structure determination and analysis at 2.77 A and 1.9 A resolutions, respectively, molecular replacement using the structure of GtfD (PDB ID 1rrv) as the search model, modeling
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Y642P
S801A/L802A/V804A/K812A/E816K/S849A/N892D
additional information
Y642P
amino acid substitution within the GRAM domain abolished this association as well as micropexophagy
Y642P
-
amino acid substitution within the GRAM domain abolished this association as well as micropexophagy
-
S801A/L802A/V804A/K812A/E816K/S849A/N892D
site-directed mutagenesis, the changed unique interaction network in mutant M7_1 with an 1800fold activity improvement toward an unnatural substrate protopanaxadiol (PPD), might influence its substrate preference
S801A/L802A/V804A/K812A/E816K/S849A/N892D
-
site-directed mutagenesis, the changed unique interaction network in mutant M7_1 with an 1800fold activity improvement toward an unnatural substrate protopanaxadiol (PPD), might influence its substrate preference
-
additional information
mutation of isoform UGT80B1 principally alters embryonic development and seed suberin accumulation and cutin formation in the seed coat, leading to abnormal permeability and tetrazolium salt uptake, mutations in UGT80A2 and USG80B1 genes result in reduced seed size, transparent testa, and salt uptake phenotypes
additional information
a T-DNA insertion mutant of UGT713B1/At5g24750 shows a phenotype that seems to be indistinguishable from Col-0 wild-type. A homozygous mutant for ugt713B1, mutation in the in the 5' UTR, seems to express mRNA in semi-quantitative RT-PCR experiments. Construction of an ugt80A2,713B1 double mutant which has a similar sterol profile to the ugt80A2 single mutant showing reduced steryl glucoside content
additional information
a T-DNA insertion mutant of UGT713B1/At5g24750 shows a phenotype that seems to be indistinguishable from Col-0 wild-type. A homozygous mutant for ugt713B1, mutation in the in the 5' UTR, seems to express mRNA in semi-quantitative RT-PCR experiments. Construction of an ugt80A2,713B1 double mutant which has a similar sterol profile to the ugt80A2 single mutant showing reduced steryl glucoside content
additional information
a T-DNA insertion mutant of UGT713B1/At5g24750 shows a phenotype that seems to be indistinguishable from Col-0 wild-type. A homozygous mutant for ugt713B1, mutation in the in the 5' UTR, seems to express mRNA in semi-quantitative RT-PCR experiments. Construction of an ugt80A2,713B1 double mutant which has a similar sterol profile to the ugt80A2 single mutant showing reduced steryl glucoside content
additional information
-
a T-DNA insertion mutant of UGT713B1/At5g24750 shows a phenotype that seems to be indistinguishable from Col-0 wild-type. A homozygous mutant for ugt713B1, mutation in the in the 5' UTR, seems to express mRNA in semi-quantitative RT-PCR experiments. Construction of an ugt80A2,713B1 double mutant which has a similar sterol profile to the ugt80A2 single mutant showing reduced steryl glucoside content
additional information
construction of ugt80A2,713B1 and ugt80A2,B1 double mutants which have similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
construction of ugt80A2,713B1 and ugt80A2,B1 double mutants which have similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
construction of ugt80A2,713B1 and ugt80A2,B1 double mutants which have similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
-
construction of ugt80A2,713B1 and ugt80A2,B1 double mutants which have similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
homozygous mutants for ugt80B1, mutation at the exon-intron boundary following the second exon, is a mRNA knockdown allel. Construction of ugt80A2,B1 double mutant which has a similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
homozygous mutants for ugt80B1, mutation at the exon-intron boundary following the second exon, is a mRNA knockdown allel. Construction of ugt80A2,B1 double mutant which has a similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
homozygous mutants for ugt80B1, mutation at the exon-intron boundary following the second exon, is a mRNA knockdown allel. Construction of ugt80A2,B1 double mutant which has a similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
-
homozygous mutants for ugt80B1, mutation at the exon-intron boundary following the second exon, is a mRNA knockdown allel. Construction of ugt80A2,B1 double mutant which has a similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
additional information
generation of a ugt80A2;B1 double mutant that is more resistant to infection by Bortrytis cinerea than the wild-type and shows increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. Mutant phenotype, overview
additional information
generation of a ugt80A2;B1 double mutant that is more resistant to infection by Bortrytis cinerea than the wild-type and shows increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. Mutant phenotype, overview
additional information
generation of a ugt80A2;B1 double mutant that is more resistant ot infection by Bortrytis cinerea than the wild-type and shows increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level, Mutant phenotype, overview
additional information
generation of a ugt80A2;B1 double mutant that is more resistant ot infection by Bortrytis cinerea than the wild-type and shows increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level, Mutant phenotype, overview
additional information
generation of ugt80B1 mutants. Sterol glucosides patterns of wild-type and mutant roots, overview. The ugt80B1 mutant shows a significant reduction in stigmasteryl glucosides only. Root hair patterning and GL2 expression are aberrant in ugt80B1 mutants. Expression of upstream cell fate regulators, SCM and WER, is altered in ugt80B1 mutants. Phenotype, overview. The ugt80B1 mutant phenotype is complemented with pro35S:UGT80B1:GFP or with proUGT80B1:UGT80B1:GFP as observed by rescue of the transparent testa phenotype
additional information
-
a T-DNA insertion mutant of UGT713B1/At5g24750 shows a phenotype that seems to be indistinguishable from Col-0 wild-type. A homozygous mutant for ugt713B1, mutation in the in the 5' UTR, seems to express mRNA in semi-quantitative RT-PCR experiments. Construction of an ugt80A2,713B1 double mutant which has a similar sterol profile to the ugt80A2 single mutant showing reduced steryl glucoside content
-
additional information
-
construction of ugt80A2,713B1 and ugt80A2,B1 double mutants which have similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
-
additional information
-
homozygous mutants for ugt80B1, mutation at the exon-intron boundary following the second exon, is a mRNA knockdown allel. Construction of ugt80A2,B1 double mutant which has a similar sterol profiles to the ugt80A2 and ugt80B1 single mutants, respectively, showing reduced steryl glucoside content
-
additional information
-
generation of ugt80B1 mutants. Sterol glucosides patterns of wild-type and mutant roots, overview. The ugt80B1 mutant shows a significant reduction in stigmasteryl glucosides only. Root hair patterning and GL2 expression are aberrant in ugt80B1 mutants. Expression of upstream cell fate regulators, SCM and WER, is altered in ugt80B1 mutants. Phenotype, overview. The ugt80B1 mutant phenotype is complemented with pro35S:UGT80B1:GFP or with proUGT80B1:UGT80B1:GFP as observed by rescue of the transparent testa phenotype
-
additional information
Arabidopsis thaliana Ws-0
-
generation of a ugt80A2;B1 double mutant that is more resistant to infection by Bortrytis cinerea than the wild-type and shows increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level. Mutant phenotype, overview
-
additional information
Arabidopsis thaliana Ws-0
-
generation of a ugt80A2;B1 double mutant that is more resistant ot infection by Bortrytis cinerea than the wild-type and shows increased levels of jasmonic acid (JA) and upregulation of two marker genes (PDF1.2 and PR4) of the ERF branch of the JA signaling pathway. The mutant also accumulates higher levels of camalexin, the major Arabidopsis thaliana phytoalexin, than wild-type plants. Camalexin accumulation correlates with enhanced transcript levels of several cytochrome P450 camalexin biosynthetic genes, as well as of their transcriptional regulators WRKY33, ANAC042, and MYB51, suggesting that the Botrytis-induced accumulation of camalexin is coordinately regulated at the transcriptional level, Mutant phenotype, overview
-
additional information
null mutant by deletion of ugt52 gene with no enzyme activity
additional information
-
null mutant by deletion of ugt52 gene with no enzyme activity
additional information
-
WsGTL1 transgenic plants overexpressing the enzyme display a number of alterations at phenotypic and metabolic level in comparison to wild-type plants which include: (1) early and enhanced growth with leaf expansion and increase in number of stomata, (2) increased production of glycowithanolide (majorly withanoside V) and campesterol, stigmasterol and sitosterol in glycosylated forms with reduced accumulation of withanolides (withaferin A, withanolide A and withanone), (3) tolerance towards biotic stress, improved survival capacity under abiotic stress (cold stress), (4) enhanced recovery capacity after cold stress, as indicated by better photosynthesis performance, chlorophyll, anthocyanin content and better quenching regulation of PSI and PSII, phenotype overview
additional information
-
analysis of the function of SGTs by virus-induced gene silencing (VIGS) of SGTL1, SGTL2 and SGTL4 in Withania somnifera. Phenotype, overview
additional information
analysis of the function of SGTs by virus-induced gene silencing (VIGS) of SGTL1, SGTL2 and SGTL4 in Withania somnifera. Phenotype, overview
additional information
analysis of the function of SGTs by virus-induced gene silencing (VIGS) of SGTL1, SGTL2 and SGTL4 in Withania somnifera. Phenotype, overview
additional information
-
analysis of the function of SGTs by virus-induced gene silencing (VIGS) of SGTL1, SGTL2 and SGTL4 in Withania somnifer. Phenotype, overview
additional information
analysis of the function of SGTs by virus-induced gene silencing (VIGS) of SGTL1, SGTL2 and SGTL4 in Withania somnifer. Phenotype, overview
additional information
analysis of the function of SGTs by virus-induced gene silencing (VIGS) of SGTL1, SGTL2 and SGTL4 in Withania somnifer. Phenotype, overview
additional information
-
WsGTL1 transgenic plants overexpressing the enzyme display a number of alterations at phenotypic and metabolic level in comparison to wild-type plants which include: (1) early and enhanced growth with leaf expansion and increase in number of stomata, (2) increased production of glycowithanolide (majorly withanoside V) and campesterol, stigmasterol and sitosterol in glycosylated forms with reduced accumulation of withanolides (withaferin A, withanolide A and withanone), (3) tolerance towards biotic stress, improved survival capacity under abiotic stress (cold stress), (4) enhanced recovery capacity after cold stress, as indicated by better photosynthesis performance, chlorophyll, anthocyanin content and better quenching regulation of PSI and PSII, phenotype overview
-
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.2
7.5
8.5
7.5
-
Tris-HCl 0.1 M, EDTA 10 mM, 2-mercaptoethanol 1 mM, sodium-deoxycholate 0.1%
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ethanol
-
increases activity between 7-15% but decreases activity above 20%
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Cibacron Blue column, Sephadex G-25 column and Mono Q anion exchange column
-
partial purification by differential centrifugation
partial purification by differential centrifugation and DEAE-cellulose column
partial purification by differential centrifugation and gel filtration on Sephadex G-100
-
partial purification by differential centrifugation and precipitation with Me2CO
-
partial purification by differential centrifugation and Sephadex G-100 column, DEAE-Sephadex A-50 and Sepharose6B
-
partially purified by gel filtration chromatography, resutlts in 3.73fold purification
purification about 12500fold by microsomes, extraction with diethyl ether, Q-Sepharose fast flow column, Hitrap blue column and SDS-PAGE
-
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant N-terminally His6-tagged UGT51 residues 722-1198 Escherichia coli srain BL21(DE3) CodonPlus by nickel affinity chromatography, tag cleavage by thrombin, followed by a secons step of nickel affinity chromatography, gel filtration, and ultrafiltration
partial purification by differential centrifugation and DEAE-cellulose column
-
partial purification by differential centrifugation and DEAE-cellulose column
-
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene AGT26, recombinant expression of N-terminally His6-tagged UGT51 residues 722-1198 from pET28a vector with a thrombin cleavage site in Escherichia coli srain BL21(DE3) CodonPlus
gene At1g43620, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression pattern
gene At3g07020, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression pattern. UGT80A2 expressing cells displays a greater than 100fold increase in cholesteryl glucoside production compared with the negative control
gene At5g24750, cDNA library screening, DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain YLR189C microsomal fraction, quantitative real-time PCR enzyme expression analysis and gene expression patterns. UGT713B1 displays little or no activity
gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene GhSGT1, DNA and amino acid sequence determination and analysis, the expression level of gene GhSGT1 is almost 10fold higher than that of gene GhSGT2
gene GhSGT2, DNA and amino acid sequence determination and analysis, the expression level of gene GhSGT1 is almost 10fold higher than that of gene GhSGT2
gene PGUG_04770, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene SGT1, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1
gene SGT2, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1
gene SGT3, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1
gene SGT4, DNA and amino acid sequence determination and analysis, comparison of Solanum lycopersicum SGT isozyme sequences and phylogenetic tree, recombinant expression of GST-tagged SlSGT protein in Escherichia coli strain BL21(DE3), and recombinant expression of YFP-tagged isozyme in Nicotiana benthamiana leaves via transfection through Agrobacterium tumefaciens strain C58C1
gene UGT80A2, quantitative reverse-transcription PCR expression analysis
gene UGT80B1, quantitative reverse-transcription PCR expression analysis
gene UGT80B1, recombinant expression of native promoter and 35 S driven GFP-tagged wild-type and mutant enzymes, the ugt80B1 mutant phenotype is complemented with pro35S:UGT80B1:GFP or with proUGT80B1:UGT80B1:GFP as observed by rescue of the transparent testa phenotype
gene WsSGTL1, ectopic overexpression in Withania somnifera using Agrobacterium tumefaciens transfection method, quantitative real-time PCR enzyme expression analysis. Overexpression of WsSGTL1 gene which is responsible for increase in glycosylated withanolide and sterols, and confers better growth and tolerance to both biotic and abiotic stresses
-
the open reading frame of SGTL1 is expressed in Escherichia coli strain DH5alpha
gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
SlSGT2 expression increases markedly in response to osmotic, salt, and cold stress, as well as upon treatment with abscisic acid and methyl jasmonate
SlSGT4 expression increases markedly in response to osmotic, salt, and cold stress, as well as upon treatment with abscisic acid and methyl jasmonate
transcriptional profiling of SlSGT gene expression in response to stress, overview
transcriptional profiling of SlSGT gene expression in response to stress, overview. SlSGT1 expression remains almost unaltered under the tested stress conditions
transcriptional profiling of SlSGT gene expression in response to stress, overview. SlSGT3 expression remains almost unaltered under the tested stress conditions
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
synthesis
-
synthesis of steryl beta-D-monoglucosides that are constituents of the cell membrane of higher plants
synthesis
-
synthesis of steryl beta-D-monoglucosides possible function as glucose carriers through the membrane and intercellular transport of sterols
synthesis
-
synthesis of poriferasterol monoglucoside may be involved in differentiation
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kalinowska, M.; Wojciechowski, Z.A.
Modulation of activities of steryl glucoside hydrolase and UDPG:sterol glucosyltransferase from Sinapis alba by detergents and lipids
Phytochemistry
25
45-49
1986
Sinapis alba
-
brenda
Kalinowska, M.; Wojciechowski, Z.A.
Enzymatic synthesis of nuatigenin 3-beta-D-glucoside in oat (Avena sativa) leaves
Phytochemistry
25
2525-2529
1986
Avena sativa
-
brenda
Kalinowska, M.; Wojciechowski, Z.A.
Subcellular localization of UDPG:nuatigenin glucosyltransferase in oat leaves
Phytochemistry
26
353-357
1987
Avena sativa
-
brenda
Wojciechowski, J.; Zimowski, J.; Tyski, S.
Enzymatic synthesis of steryl 3-beta-D-monoglucosides in the slime mold Physarum polycephalum
Phytochemistry
16
911-914
1977
Badhamia polycephala
-
brenda
Murakami-Murofushi, K.; Ohta, J.
Expression of UDP-glucose: poriferasterol glucosyltransferase in the process of differentiation of a true slime mold, Physarum polycephalum
Biochim. Biophys. Acta
992
412-415
1989
Badhamia polycephala
brenda
Ullmann, P.; Bouvier-Nave, P.; Benveniste, P.
Regulation by phospholipids and kinetic studies of plant membrane-bound UDP-glucose sterol beta-D-glucosyl transferase
Plant Physiol.
85
51-55
1987
Zea mays
brenda
Esders, T.W.; Light, R.J.
Occurrence of a uridine diphosphate glucose: sterol glucosyltransferase in Candida bogoriensis
J. Biol. Chem.
247
7494-7497
1972
Pseudohyphozyma bogoriensis
brenda
Yoshikawa, T.; Furuya, T.
Purification and properties of sterol:UDPG glucosyltransferase in cell culture of Digitalis purpurea
Phytochemistry
18
239-241
1979
Digitalis purpurea
-
brenda
Wojciechowski, Z.A.; Zimowski, J.; Zimowski, J.G.; Lyznik, A.
Specificity of sterol-glucosylating enzymes from Sinapis alba and Physarum polycephalum
Biochim. Biophys. Acta
570
363-370
1979
Badhamia polycephala, Sinapis alba
brenda
Ury, A.; Benveniste, P.; Bouvier-Nave, P.
Phospholipid dependence of plant UDP-glucose sterol beta-D-glucosyl transferase
Plant Physiol.
91
567-573
1989
Zea mays
brenda
Paczkowski, C.; Zimowski, J.; Krawczyk, D.; Wojciechowski, Z.A.
Steroid-specific glucosyltransferase in Asparagus plumosus shoots
Phytochemistry
29
63-70
1990
Asparagus setaceus
-
brenda
Fang, T.Y.; Baisted, D.J.
UDPG:sterol glucosyltransferase in etiolated pea seedlings
Phytochemistry
15
273-278
1976
Lathyrus oleraceus
-
brenda
Staver, M.J.; Glick, K.; Baisted, D.J.
Uridine diphosphate glucose-sterol glucosyltransferase and nucleoside diphosphatase activities in etiolated pea seedlings
Biochem. J.
169
297-303
1978
Lathyrus oleraceus
brenda
Forsee, W.T.; Laine, R.A.; Elbein, A.D.
Solubilization of a particulate UDP-Glucose:sterol beta-glucosyltransferase in developing cotton fibers and seeds and characterization of steryl 6-acyl-D-glucosides
Arch. Biochem. Biophys.
161
248-259
1974
Gossypium hirsutum
-
brenda
Bouvier-Nave, P.; Ullmann, P.; Rimmele, D.; Benveniste, P.
Phospholipid-dependence of plant UDP-glucose-sterol-beta-D-glucosyltransferase. I. Detergent-mediated delipidatio by selective solubilization
Plant Sci. Lett.
36
19-27
1984
Zea mays
-
brenda
Quantin-Martenot, E.; Benveniste, P.; Hartmann, M.A.; Bouvier-Nave, P.
Activation of etiolated maize coleoptiles plasma membrane-bound uridine-diphosphate-glucose-sterol-beta-D-glucosyltransferase by triton X-100, hydroxyl ions and phospholipase A2
Plant Sci. Lett.
29
305-314
1983
Zea mays
-
brenda
Ullmann, P.; Rimmele, D.; Benveniste, P.; Bouvier-Nave, P.
Phospholipid-dependence of plant UDP-glucose-sterol-beta-D-glucosyltransferase. II. Acetone-mediated delipidation and kinetic studies
Plant Sci. Lett.
36
29-36
1984
Zea mays
-
brenda
Ullmann, P.; Ury, A.; Rimmele, D.; Benveniste, P.; Bouvier-Nave, P.
UDP-glucose sterol beta-D-glucosyltransferase, a plasma membrane-bound enzyme of plants: Enzymic properties and lipid dependence
Biochimie
75
713-723
1993
Zea mays
brenda
Warnecke, D.C.; Heinz, E.
Purification of a membrane-bound UDP-glucose:sterol beta-D-glucosyltransferase based on its solubility in diethyl ether
Plant Physiol.
105
1067-1073
1994
Avena sativa
brenda
Warnecke, D.C.; Baltrusch, M.; Buck, F.; Wolter, F.P.; Heinz, E.
UDP-glucose:sterol glucosyltransferase: cloning and functional expression in Escherichia coli
Plant Mol. Biol.
35
597-603
1997
Arabidopsis thaliana (Q9M8Z7), Avena sativa (O22678), Avena sativa ugt80A1 (O22678)
brenda
Warnecke, D.; Erdmann, R.; Fahl, A.; Hube, B.; Muller, F.; Zank, T.; Zahringer, U.; Heinz, E.
Cloning and functional expression of UGT genes encoding sterol glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideum
J. Biol. Chem.
274
13048-13059
1999
Avena sativa (O22678), Avena sativa ugt80A1 (O22678), Candida albicans (Q5A950), Candida albicans, Dictyostelium discoideum (Q54IL5), Dictyostelium discoideum, Dictyostelium discoideum ugt52 (Q54IL5), Komagataella pastoris, Komagataella pastoris (Q9Y751), Komagataella pastoris GS115 (Q9Y751), Saccharomyces cerevisiae (Q06321), Saccharomyces cerevisiae
brenda
Bergenstraahle, A.; Tillberg, E.; Jonsson, L.
Effects of ethephon and norbornadiene on sterol and glycoalkaloid biosynthesis in potato tuber disks
Physiol. Plant.
89
301-308
1993
Solanum tuberosum
-
brenda
Stasyk, O.V.; Nazarko, T.Y.; Stasyk, O.G.; Krasovska, O.S.; Warnecke, D.; Nicaud, J.M.; Cregg, J.M.; Sibirny, A.A.
Sterol glucosyltransferases have different functional roles in Pichia pastoris and Yarrowia lipolytica
Cell Biol. Int.
27
947-952
2003
Komagataella pastoris, Yarrowia lipolytica
brenda
Oku, M.; Warnecke, D.; Noda, T.; Muller, F.; Heinz, E.; Mukaiyama, H.; Kato, N.; Sakai, Y.
Peroxisome degradation requires catalytically active sterol glucosyltransferase with a GRAM domain
EMBO J.
22
3231-3241
2003
Komagataella pastoris (Q9Y751), Komagataella pastoris, Komagataella pastoris GS115 (Q9Y751)
brenda
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Characterisation of neutral trehalase and UDP-glucose:sterol glucosyltransferase genes from the plant pathogenic fungus Leptosphaeria maculans
Physiol. Mol. Plant Pathol.
62
305-313
2003
Plenodomus lingam
-
brenda
Kim, Y.K.; Wang, Y.; Liu, Z.M.; Kolattukudy, P.E.
Identification of a hard surface contact-induced gene in Colletotrichum gloeosporioides conidia as a sterol glycosyl transferase, a novel fungal virulence factor
Plant J.
30
177-187
2002
Colletotrichum gloeosporioides
brenda
Sharma, L.K.; Madina, B.R.; Chaturvedi, P.; Sangwan, R.S.; Tuli, R.
Molecular cloning and characterization of one member of 3beta-hydroxy sterol glucosyltransferase gene family in Withania somnifera
Arch. Biochem. Biophys.
460
48-55
2007
Arabidopsis thaliana, Withania somnifera (Q2I015), Withania somnifera
brenda
Madina, B.R.; Sharma, L.K.; Chaturvedi, P.; Sangwan, R.S.; Tuli, R.
Purification and physico-kinetic characterization of 3beta-hydroxy specific sterol glucosyltransferase from Withania somnifera (L) and its stress response
Biochim. Biophys. Acta
1774
392-402
2007
Withania somnifera
brenda
Potocka, A.; Zimowski, J.
Metabolism of conjugated sterols in eggplant. Part 1. UDP-glucose:sterol glucosyltransferase
Acta Biochim. Pol.
55
127-134
2008
Solanum melongena
brenda
Cao, Y.; Klionsky, D.J.
Atg26 is not involved in autophagy-related pathways in Saccharomyces cerevisiae
Autophagy
3
17-20
2007
Saccharomyces cerevisiae (Q06321), Saccharomyces cerevisiae
brenda
Asakura, M.; Ninomiya, S.; Sugimoto, M.; Oku, M.; Yamashita, S.; Okuno, T.; Sakai, Y.; Takano, Y.
Atg26-mediated pexophagy is required for host invasion by the plant pathogenic fungus colletotrichum orbiculare
Plant Cell
21
1291-1304
2009
Colletotrichum orbiculare
brenda
DeBolt, S.; Scheible, W.R.; Schrick, K.; Auer, M.; Beisson, F.; Bischoff, V.; Bouvier-Nave, P.; Carroll, A.; Hematy, K.; Li, Y.; Milne, J.; Nair, M.; Schaller, H.; Zemla, M.; Somerville, C.
Mutations in UDP-glucose:sterol glucosyltransferase in Arabidopsis cause transparent testa phenotype and suberization defect in seeds
Plant Physiol.
151
78-87
2009
Arabidopsis thaliana (Q9M8Z7)
brenda
Akiyama, H.; Hamada, T.; Nagatsuka, Y.; Kobayashi, S.; Hirabayashi, Y.; Murakami-Murofushi, K.
A possible mechanism of cholesteryl glucoside formation involved in heat shock response in the animal cell membrane
Cytologia
76
19-25
2011
Homo sapiens
-
brenda
Chaturvedi, P.; Mishra, M.; Akhtar, N.; Gupta, P.; Mishra, P.; Tuli, R.
Sterol glycosyltransferases-identification of members of gene family and their role in stress in Withania somnifera
Mol. Biol. Rep.
39
9755-9764
2012
Saccharomyces cerevisiae (Q06321)
brenda
Stucky, D.F.; Arpin, J.C.; Schrick, K.
Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis
J. Exp. Bot.
66
189-201
2015
Arabidopsis thaliana (Q9M8Z7), Arabidopsis thaliana (F4KII1), Arabidopsis thaliana (Q9XIG1), Arabidopsis thaliana, Arabidopsis thaliana Col-0 (F4KII1), Arabidopsis thaliana Col-0 (Q9M8Z7), Arabidopsis thaliana Col-0 (Q9XIG1)
brenda
Malik, V.; Zhang, M.; Dover, L.G.; Northen, J.S.; Flinn, A.; Perry, J.J.; Black, G.W.
Sterol 3beta-glucosyltransferase biocatalysts with a range of selectivities, including selectivity for testosterone
Mol. Biosyst.
9
2816-2822
2013
Debaryomyces hansenii (Q6BN88), Debaryomyces hansenii CBS 767 (Q6BN88), Kluyveromyces lactis (Q6CUV2), Kluyveromyces lactis DSM 70799 (Q6CUV2), Komagataella pastoris (Q9Y751), Komagataella pastoris GS115 (Q9Y751), Meyerozyma guilliermondii (A5DNB9), Meyerozyma guilliermondii DSM 6381 (A5DNB9), Ogataea angusta (A7KAK6), Ogataea angusta DSM 70277 (A7KAK6), Saccharomyces cerevisiae (Q06321), Saccharomyces cerevisiae ATCC 204508 (Q06321)
brenda
Saema, S.; Rahman, L.U.; Singh, R.; Niranjan, A.; Ahmad, I.Z.; Misra, P.
Ectopic overexpression of WsSGTL1, a sterol glucosyltransferase gene in Withania somnifera, promotes growth, enhances glycowithanolide and provides tolerance to abiotic and biotic stresses
Plant Cell Rep.
35
195-211
2016
Withania somnifera, Withania somnifera NMITLI-101
brenda
Li, X.; Xia, T.; Huang, J.; Guo, K.; Liu, X.; Chen, T.; Xu, W.; Wang, X.; Feng, S.; Peng, L.
Distinct biochemical activities and heat shock responses of two UDP-glucose sterol glucosyltransferases in cotton
Plant Sci.
219-220
1-8
2014
Gossypium hirsutum (A0A023NGA8), Gossypium hirsutum (A0A023NFQ4), Gossypium hirsutum, Gossypium hirsutum Huamian99 (A0A023NGA8), Gossypium hirsutum Huamian99 (A0A023NFQ4)
brenda
Castillo, N.; Pastor, V.; Chavez, A.; Arro, M.; Boronat, A.; Flors, V.; Ferrer, A.; Altabella, T.
Inactivation of UDP-glucose sterol glucosyltransferases enhances Arabidopsis resistance to Botrytis cinerea
Front. Plant Sci.
10
1162
2019
Arabidopsis thaliana (Q9M8Z7), Arabidopsis thaliana (Q9XIG1), Arabidopsis thaliana Ws-0 (Q9M8Z7), Arabidopsis thaliana Ws-0 (Q9XIG1)
brenda
Ramirez-Estrada, K.; Castillo, N.; Lara, J.A.; Arro, M.; Boronat, A.; Ferrer, A.; Altabella, T.
Tomato UDP-glucose sterol glycosyltransferases a family of developmental and stress regulated genes that encode cytosolic and membrane-associated forms of the enzyme
Front. Plant Sci.
8
984
2017
Solanum lycopersicum (K4C3H8), Solanum lycopersicum (A0A2D1N4Z8), Solanum lycopersicum (K4BTE6), Solanum lycopersicum (K4BS77), Solanum lycopersicum
brenda
Chen, L.; Zhang, Y.; Feng, Y.
Structural dissection of sterol glycosyltransferase UGT51 from Saccharomyces cerevisiae for substrate specificity
J. Struct. Biol.
204
371-379
2018
Saccharomyces cerevisiae (Q06321), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (Q06321)
brenda
Singh, G.; Tiwari, M.; Singh, S.P.; Singh, S.; Trivedi, P.K.; Misra, P.
Silencing of sterol glycosyltransferases modulates the withanolide biosynthesis and leads to compromised basal immunity of Withania somnifera
Sci. Rep.
6
25562
2016
Withania somnifera, Withania somnifera (Q2I015), Withania somnifera (Q2I014)
brenda
Pook, V.G.; Nair, M.; Ryu, K.; Arpin, J.C.; Schiefelbein, J.; Schrick, K.; DeBolt, S.
Positioning of the SCRAMBLED receptor requires UDP-Glc sterol glucosyltransferase 80B1 in Arabidopsis roots
Sci. Rep.
7
5714
2017
Arabidopsis thaliana (Q9XIG1), Arabidopsis thaliana Col-0 (Q9XIG1)
brenda
Gao, J.; Xu, Y.; Hua, C.; Li, C.; Zhang, Y.
Molecular cloning and functional characterization of a sterol 3-O-glucosyltransferase involved in biosynthesis of steroidal saponins in Trigonella foenum-graecum
Front. Plant Sci.
12
809579
2021
Trigonella foenum-graecum
brenda
Cisneros, A.E.; Lison, P.; Campos, L.; Lopez-Tubau, J.M.; Altabella, T.; Ferrer, A.; Daros, J.A.; Carbonell, A.
Down-regulation of tomato STEROL GLYCOSYLTRANSFERASE 1 perturbs plant development and facilitates viroid infection
J. Exp. Bot.
74
1564-1578
2023
Solanum lycopersicum (K4C3H8)
brenda
He, M.; Guo, S.; Yin, Y.; Zhang, C.; Zhang, X.
A novel sterol glycosyltransferase catalyses steroidal sapogenin 3-O glucosylation from Paris polyphylla var. yunnanensis
Mol. Biol. Rep.
50
2137-2146
2023
Paris polyphylla var. yunnanensis (A0A9Y1L8F8)
brenda
Xu, W.K.; Zhao, C.X.; Yang, X.W.; Chen, Y.G.; Long, L.P.; Yan, Y.F.; Guo, K.; Li, S.H.; Liu, Y.
Characterization of a glycosyltransferase from Paris polyphylla for application in biosynthesis of rare ophiopogonins and ginsenosides
Phytochemistry
225
114173
2024
Paris polyphylla var. yunnanensis
brenda
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