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Information on EC 2.4.1.162 - aldose beta-D-fructosyltransferase
for references in articles please use BRENDA:EC2.4.1.162
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EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.162 aldose beta-D-fructosyltransferase
The enzyme appears in viruses and cellular organisms
- 2.4.1.162
- fructans
- streptococcus
- mutans
- fructooligosaccharides
- 1-kestose
- inulin
-
prebiotic
- salivarius
-
transfructosylation
-
glucosyltransferases
- levans
- levansucrase
- lolium
-
sucrose:sucrose
- perenne
-
cariogenic
- nystose
-
sucrose:fructan
- 6g-fft
- inulosucrase
- invertases
- ryegrass
-
inulin-type
-
pectinex
-
sucrose-containing
- chicory
-
sucrose-dependent
- sobrinus
-
fructan:fructan
- beta-fructofuranosidase
Reaction Schemes
showSynonyms
fructosyltransferase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
beta-fructofuranoside, acceptor (aldose) fructosyltransferase
beta-fructosyl transferase
fructosyltransferase
FTF
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
alpha-D-aldosyl1 beta-D-fructoside + D-aldose2 = D-aldose1 + alpha-D-aldosyl2 beta-D-fructoside
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
alpha-D-aldosyl-beta-D-fructoside:aldose 1-beta-D-fructosyltransferase
-
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CAS REGISTRY NUMBER
COMMENTARY
9031-67-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1',6'-dichlorosucrose + fructose acceptor
?
-
Substrates: 1',6'-dichlorosucrose has some donor activity
Products: -
Products: -
?
6,1',6'-trichlorosucrose + fructose acceptor
?
-
Substrates: 6, 1',6'-trichlorosucrose has some donor activity
Products: -
Products: -
?
6-deoxy-D-glucose + sucrose
6-deoxysucrose + D-glucose
-
Substrates: -
Products: -
Products: -
?
alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl-(1->2)-D-fructofuranoside + sucrose
alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranoside + ?
D-glucose 6-benzoate + sucrose
sucrose 6-benzoate + D-glucose
-
Substrates: -
Products: -
Products: -
?
D-sucrose + D-galactose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2,1)-D-6-O-acetyl-galactopyranoside
-
Substrates: -
Products: -
Products: -
?
D-sucrose + D-gluconic acid
D-glucose + beta-D-fructofuranosyl-(2,1)-D-gluconic acid
-
Substrates: -
Products: -
Products: -
?
glucose 6-acetate + sucrose
sucrose 6-acetate + D-glucose
-
Substrates: -
Products: -
Products: -
?
raffinose + D-xylose
beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
raffinose + galactose
beta-D-fructofuranosyl-2,1-D-galactopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
raffinose + xylose
beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 3-O-methyl alpha-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-alpha-D-glucopyranoside
sucrose + 3-O-methyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2,1)-3-O-methyl-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 3-O-methyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-D-glucopyranoside
sucrose + 4-chloro-4-deoxy-D-galactose
D-glucose + beta-D-fructofuranosyl-(2->1)-4-deoxy-4-chloro-D-galactopyranoside
sucrose + 4-chloro-4-deoxy-D-glucose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2->1)-4-chloro-4-deoxy-D-glucopyranoside 6-acetate
sucrose + 6-chloro-6-deoxy-D-glucose
D-glucose + beta-D-fructofuranosyl-(2,1)-6-chloro-6-deoxy-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-chloro-6-deoxy-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-chloro-6-deoxy-D-glucoside
sucrose + 6-deoxy-D-glucose
6-deoxysucrose + D-glucose
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-O-benzyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-benzyl-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-O-benzyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-benzyl-D-glucoside
sucrose + 6-O-methyl-D-galactose
D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-galactoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-O-methyl-D-galactose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-methyl-D-galactoside
sucrose + 6-O-methyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-O-methyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)--6-O-methyl-D-glucoside
sucrose + cellobiose
D-glucose + beta-D-fructofuranosyl-(2,1)-cellobioside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-arabinose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-arabinoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-fucose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-fucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-galactose
beta-D-fructofuranosyl-2,1-D-galactopyranoside + D-glucose
-
Substrates: -
Products: -
Products: -
?
sucrose + D-galactose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-galactopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-galactose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2->1)-D-6-O-acetyl-galactopyranoside
sucrose + D-glucose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-acetyl-D-glucoside
sucrose + D-glucose 6-acetate
sucrose 6-acetate + D-glucose
-
Substrates: -
Products: -
Products: -
?
sucrose + D-glucose 6-phosphate
D-glucose + beta-D-fructofuranosyl-(2->1)-D-glucopyranoside 6-phosphate
sucrose + D-glucose 6-phosphate
sucrose 6-phosphate + D-glucose
-
Substrates: -
Products: -
Products: -
?
sucrose + D-lactose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-lactoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-lyxose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-lyxoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-maltose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-maltoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-mannose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-mannopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-ribose
D-glucose + beta-D-fructofuranosyl-(2,1)-D-riboside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-xylose
D-glucose + beta-D-fructofuranosyl-(2,1)-alpha-D-xylopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + isomaltose
D-glucose + beta-D-fructofuranosyl-(2,1)-isomaltoside
-
Substrates: -
Products: -
Products: -
?
sucrose + L-arabinose
D-glucose + beta-D-fructofuranosyl-(2,1)-L-arabinoside
-
Substrates: -
Products: -
Products: -
?
sucrose + L-rhamnose
D-glucose + beta-D-fructofuranosyl-(2,1)-L-rhamnoside
-
Substrates: -
Products: -
Products: -
?
sucrose + maltohexaose
D-glucose + beta-D-fructofuranosyl-(2,1)-maltohexaoside
-
Substrates: -
Products: -
Products: -
?
sucrose + maltopentaose
D-glucose + beta-D-fructofuranosyl-(2,1)-maltopentaoside
-
Substrates: -
Products: -
Products: -
?
sucrose + maltotetraose
D-glucose + beta-D-fructofuranosyl-(2,1)-maltotetraoside
-
Substrates: -
Products: -
Products: -
?
sucrose + melibiose
D-glucose + beta-D-fructofuranosyl-(2,1)-mellibioside
-
Substrates: -
Products: -
Products: -
?
sucrose + xylose
beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + D-glucose
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: when oligosaccharide acceptors are used, the acceptor activity decreases with increasing size, as in the homologous series maltose, maltotriose, maltotetrose, etc., reactivity also decreases with the size of the molecule in the homologous series sucrose, raffinose, stachyose, and verbascose, inulin and levan, and also levan that is partially hydrolysed with acid, do not act as donors, lactulose, isomaltulose, D-glucose-1-phosphate, methyl beta-D-glucoside, and D-xylulose are unreactive
Products: -
Products: -
?
alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl-(1->2)-D-fructofuranoside + sucrose
alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranoside + ?
-
Substrates: -
Products: -
Products: -
?
alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranosyl-(1->2)-D-fructofuranoside + sucrose
alpha-D-galactopyranosyl-(1->6)-O-alpha-D-glucopyranoside + ?
-
Substrates: -
Products: -
Products: -
?
fructose donor + fructose acceptor
D-aldose + D-aldosyl beta-D-fructoside
-
Substrates: the donor must be a beta-fructosyl ring attached to the anomeric carbon of an aldose by an 1, 2 link, acceptors can be monomeric or oligomeric hexoses or pentoses, no acceptors are inosital, 2-deoxyglucose, glucosamine
Products: -
Products: -
?
fructose donor + fructose acceptor
D-aldose + D-aldosyl beta-D-fructoside
-
Substrates: -
Products: the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides
Products: the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides
?
fructose donor + fructose acceptor
D-aldose + D-aldosyl beta-D-fructoside
-
Substrates: the donor must be a beta-fructosyl ring attached to the anomeric carbon of an aldose by an 1, 2 link, acceptors can be monomeric or oligomeric hexoses or pentoses, no acceptors are inosital, 2-deoxyglucose, glucosamine
Products: -
Products: -
?
fructose donor + fructose acceptor
D-aldose + D-aldosyl beta-D-fructoside
-
Substrates: -
Products: the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides
Products: the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides
?
raffinose + D-xylose
beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
raffinose + D-xylose
beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 3-O-methyl alpha-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-alpha-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 3-O-methyl alpha-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-alpha-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 3-O-methyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 3-O-methyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-3-O-methyl-D-glucopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 4-chloro-4-deoxy-D-galactose
D-glucose + beta-D-fructofuranosyl-(2->1)-4-deoxy-4-chloro-D-galactopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 4-chloro-4-deoxy-D-galactose
D-glucose + beta-D-fructofuranosyl-(2->1)-4-deoxy-4-chloro-D-galactopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 4-chloro-4-deoxy-D-glucose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2->1)-4-chloro-4-deoxy-D-glucopyranoside 6-acetate
-
Substrates: -
Products: -
Products: -
?
sucrose + 4-chloro-4-deoxy-D-glucose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2->1)-4-chloro-4-deoxy-D-glucopyranoside 6-acetate
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-chloro-6-deoxy-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-chloro-6-deoxy-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-chloro-6-deoxy-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-chloro-6-deoxy-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-deoxy-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-deoxy-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-deoxy-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-deoxy-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-O-benzyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-benzyl-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-O-benzyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-benzyl-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-O-methyl-D-galactose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-methyl-D-galactoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-O-methyl-D-galactose
D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-methyl-D-galactoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-O-methyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)--6-O-methyl-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + 6-O-methyl-D-glucose
D-glucose + beta-D-fructofuranosyl-(2->1)--6-O-methyl-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + cellobiose
D-glucose + beta-D-fructofuranosyl-(2->1)-cellobioside
-
Substrates: -
Products: -
Products: -
?
sucrose + cellobiose
D-glucose + beta-D-fructofuranosyl-(2->1)-cellobioside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-arabinose
D-glucose + beta-D-fructofuranosyl-(2->1)-D-arabinoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-arabinose
D-glucose + beta-D-fructofuranosyl-(2->1)-D-arabinoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-galactose
D-glucose + beta-D-fructofuranosyl-(2->1)-D-galactopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-galactose
D-glucose + beta-D-fructofuranosyl-(2->1)-D-galactopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-galactose
galactosylfructoside + D-glucose
-
Substrates: -
Products: -
Products: -
?
sucrose + D-galactose
galactosylfructoside + D-glucose
-
Substrates: -
Products: -
Products: -
?
sucrose + D-galactose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2->1)-D-6-O-acetyl-galactopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-galactose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2->1)-D-6-O-acetyl-galactopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-gluconic acid
D-glucose + beta-D-fructofuranosyl-(2->1)-D-gluconic acid
-
Substrates: -
Products: -
Products: -
?
sucrose + D-gluconic acid
D-glucose + beta-D-fructofuranosyl-(2->1)-D-gluconic acid
-
Substrates: -
Products: -
Products: -
?
sucrose + D-glucose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-acetyl-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-glucose 6-acetate
D-glucose + beta-D-fructofuranosyl-(2->1)-6-O-acetyl-D-glucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-glucose 6-phosphate
D-glucose + beta-D-fructofuranosyl-(2->1)-D-glucopyranoside 6-phosphate
-
Substrates: -
Products: -
Products: -
?
sucrose + D-glucose 6-phosphate
D-glucose + beta-D-fructofuranosyl-(2->1)-D-glucopyranoside 6-phosphate
-
Substrates: -
Products: -
Products: -
?
sucrose + D-glycero-D-galactoheptose
D-glucose + ?
-
Substrates: -
Products: -
Products: -
?
sucrose + D-mannose
D-glucose + beta-D-fructofuranosyl-(2->1)-D-mannopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-mannose
D-glucose + beta-D-fructofuranosyl-(2->1)-D-mannopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-ribose
D-glucose + beta-D-fructofuranosyl-(2->1)-D-riboside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-ribose
D-glucose + beta-D-fructofuranosyl-(2->1)-D-riboside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-xylose
D-glucose + beta-D-fructofuranosyl-(2->1)-alpha-D-xylopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-xylose
D-glucose + beta-D-fructofuranosyl-(2->1)-alpha-D-xylopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-xylose
D-glucose + beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + D-xylose
D-glucose + beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside
-
Substrates: -
Products: -
Products: -
?
sucrose + ethanol
D-glucose + 2-O-ethyl-beta-D-fructofuranose
-
Substrates: -
Products: -
Products: -
?
sucrose + ethanol
D-glucose + 2-O-ethyl-beta-D-fructofuranose
-
Substrates: -
Products: -
Products: -
?
sucrose + isomaltose
D-glucose + beta-D-fructofuranosyl-(2->1)-isomaltoside
-
Substrates: -
Products: -
Products: -
?
sucrose + isomaltose
D-glucose + beta-D-fructofuranosyl-(2->1)-isomaltoside
-
Substrates: -
Products: -
Products: -
?
sucrose + L-arabinose
D-glucose + beta-D-fructofuranosyl-(2->1)-L-arabinoside
-
Substrates: -
Products: -
Products: -
?
sucrose + L-arabinose
D-glucose + beta-D-fructofuranosyl-(2->1)-L-arabinoside
-
Substrates: -
Products: -
Products: -
?
sucrose + L-fucose
D-glucose + beta-D-fructofuranosyl-(2->1)-L-fucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + L-fucose
D-glucose + beta-D-fructofuranosyl-(2->1)-L-fucoside
-
Substrates: -
Products: -
Products: -
?
sucrose + L-rhamnose
D-glucose + beta-D-fructofuranosyl-(2->1)-L-rhamnoside
-
Substrates: -
Products: -
Products: -
?
sucrose + L-rhamnose
D-glucose + beta-D-fructofuranosyl-(2->1)-L-rhamnoside
-
Substrates: -
Products: -
Products: -
?
sucrose + lactose
D-glucose + beta-D-fructofuranosyl-(2->1)-lactoside
-
Substrates: -
Products: -
Products: -
?
sucrose + lactose
D-glucose + beta-D-fructofuranosyl-(2->1)-lactoside
-
Substrates: -
Products: -
Products: -
?
sucrose + lyxose
D-glucose + beta-D-fructofuranosyl-(2->1)-lyxoside
-
Substrates: -
Products: -
Products: -
?
sucrose + lyxose
D-glucose + beta-D-fructofuranosyl-(2->1)-lyxoside
-
Substrates: -
Products: -
Products: -
?
sucrose + maltohexaose
D-glucose + beta-D-fructofuranosyl-(2->1)-maltohexaoside
-
Substrates: -
Products: -
Products: -
?
sucrose + maltohexaose
D-glucose + beta-D-fructofuranosyl-(2->1)-maltohexaoside
-
Substrates: -
Products: -
Products: -
?
sucrose + maltopentaose
D-glucose + beta-D-fructofuranosyl-(2->1)-maltopentaoside
-
Substrates: -
Products: -
Products: -
?
sucrose + maltopentaose
D-glucose + beta-D-fructofuranosyl-(2->1)-maltopentaoside
-
Substrates: -
Products: -
Products: -
?
sucrose + maltose
D-glucose + beta-D-fructofuranosyl-(2->1)-maltoside
-
Substrates: -
Products: -
Products: -
?
sucrose + maltose
D-glucose + beta-D-fructofuranosyl-(2->1)-maltoside
-
Substrates: -
Products: -
Products: -
?
sucrose + maltotriose
D-glucose + beta-D-fructofuranosyl-(2->1)-maltotrioside
-
Substrates: -
Products: -
Products: -
?
sucrose + maltotriose
D-glucose + beta-D-fructofuranosyl-(2->1)-maltotrioside
-
Substrates: -
Products: -
Products: -
?
sucrose + melibiose
D-glucose + beta-D-fructofuranosyl-(2->1)-melibioside
-
Substrates: -
Products: -
Products: -
?
sucrose + melibiose
D-glucose + beta-D-fructofuranosyl-(2->1)-melibioside
-
Substrates: -
Products: -
Products: -
?
sucrose + xylitol
D-glucose + beta-D-fructofuranosyl-(2->1)-xylitol
-
Substrates: -
Products: -
Products: -
?
sucrose + xylitol
D-glucose + beta-D-fructofuranosyl-(2->1)-xylitol
-
Substrates: -
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
fructose donor + fructose acceptor
D-aldose + D-aldosyl beta-D-fructoside
-
Substrates: -
Products: the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides
Products: the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides
?
fructose donor + fructose acceptor
D-aldose + D-aldosyl beta-D-fructoside
-
Substrates: -
Products: the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides
Products: the products can act as acceptors for further reactions, leading to oligosaccharides or polysaccharides
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
p-hydroxymercuribenzoate does not affect fructosyl transferase activity, the fructosyl transferase is completely resistant to endogenous proteases and to trypsin
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
sucrose
-
the fructosyl transferase activity in strain NCIB 11871 is induced only by sucrose, other sugars tested as inducers (glucose, fructose, maltose, and cellobiose) are ineffective
additional information
-
the fructosyl transferase is unaffected by anionic, cationic, or nonionic detergents such as SDS, cetyltrimethylammonium bromide, and Triton X-100
-
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Dehydration
Use of spent osmotic solutions for the production of fructooligosaccharides by Aspergillus oryzae N74.
Dehydration
Water stress drastically reduces root growth and inulin yield in Cichorium intybus (var. sativum) independently of photosynthesis.
Dental Caries
Biochemical characterization and evaluation of virulence of a fructosyltransferase-deficient mutant of Streptococcus mutans V403.
Dental Caries
Differential expression profiles of Streptococcus mutans ftf, gtf and vicR genes in the presence of dietary carbohydrates at early and late exponential growth phases.
Dental Caries
Immunization with dextransucrases, levansucrases, and glycosidic hydrolases from oral streptococci. II. Immunization with glucosyltransferases, fructosyltransferases, and glycosidic hydrolases from oral streptococci in monkeys.
Dental Caries
Regulation of the gtfBC and ftf genes of Streptococcus mutans in biofilms in response to pH and carbohydrate.
Periodontal Diseases
Effects of biosurfactant produced by Lactobacillus casei on gtfB, gtfC, and ftf gene expression level in S. mutans by real-time RT-PCR.
Periodontal Diseases
Effects of Lactobacillus reuteri-derived biosurfactant on the gene expression profile of essential adhesion genes (gtfB, gtfC and ftf) of Streptococcus mutans.
Stomatognathic Diseases
In vitro real-time interactions of cranberry constituents with immobilized fructosyltransferase.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
the purified enzyme is concentrated 33fold and has an activity of 32 nmol/ml/min
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE-52 cellulose column chromatography, and Mono Q column chromatography
-
recombinant reatures soluble C-terminally Strep-tagged enzyme from Escherichia coli strain BL21(DE3) Rosetta inclusion bodies by Strep-Tactin affinity chromatography and dialysis
-
recombinant soluble C-terminally Strep-tagged enzyme from Escherichia coli strain BL21 (DE3) Rosetta inclusion bodies by Strep-Tactin affinity chromatography and dialysis
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant expression of C-terminally Strep-tagged enzyme in Escherichia coli strain BL21(DE3) Rosetta in inclusion bodies
-
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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally Strep-tagged enzyme from Escherichia coli strain BL21(DE3) Rosetta inclusion bodies by ion exchange chromatography and dialysis, method optimization and evaluation of renaturation, overview. Conventional anion exchangers with gel matrix structure enhance refolding performance by about 43% with final protein concentration of 9 mg/ml and yield improvement is strictly linear dependent on the mass ratio of resins to protein, purolite ion exchange resins are then employed. With the applied setup refolded protein is self-eluted from resin due to pH and salt concentration shift during dialysis. Macroporous resins and gel filtration media show a negative effect on refolding yields
-
recombinant C-terminally Strep-tagged enzyme from Escherichia coli strain BL21(DE3) Rosetta inclusion bodies, method optimization and evaluation of a cost effective renaturation, detailed overview. The enzyme is solubilized from batch sample by detergent buffer containing 1 M urea, 0.1 M Tris, 25 mM deoxycholate, and 1% v/v IGEPAL CA-630. Refolding is done by batchwise or continuous exchange of dialysis buffer with cellulose membrane, over a period of 48 h and a buffer to sample volume ratio of 100:1 at 4°C, batch dialysis. Mild solubilization at low urea concentration (2 M) and high pH (pH 12) does not improve the recovery of protein from inclusion bodies. Phosphate buffers exhibit above-average activity yield of 1022 U/ml
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rathbone, E.B.; Hacking, A.J.; Cheetham, P.S.J.
Trihalo-trideoxy galactosucrose compounds
UK Patent Application
GB21
45080 A
1985
Bacillus subtilis, Erwinia sp.
-
brenda
Baciu, I.E.; Joerdening, H.J.; Seibel, J.; Buchholz, K.
Investigations of the transfructosylation reaction by fructosyltransferase from B. subtilis NCIMB 11871 for the synthesis of the sucrose analogue galactosyl-fructoside
J. Biotechnol.
116
347-357
2004
Bacillus subtilis, Bacillus subtilis 168
brenda
Cheetham, P.S.; Hacking, A.J.; Vlitos, M.
Synthesis of novel disaccharides by a newly isolated fructosyl transferase from Bacillus subtilis
Enzyme Microb. Technol.
11
212-219
1989
Bacillus subtilis, Bacillus subtilis NCIB 11871
-
brenda
Gabrielczyk, J.; Kluitmann, J.; Dammeyer, T.; Joerdening, H.J.
Effects of ionic strength on inclusion body refolding at high concentration
Protein Expr. Purif.
130
100-106
2017
Bacillus subtilis, Bacillus subtilis NCIMB 11871
brenda
Gabrielczyk, J.; Joerdening, H.J.
Ion exchange resins as additives for efficient protein refolding by dialysis
Protein Expr. Purif.
133
35-40
2017
Bacillus subtilis, Bacillus subtilis NCIMB 11871
brenda
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