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Synonyms
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acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine mannoside alpha1-2-
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alpha-N-acetylglucosaminyltransferase
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uridine diphosphoacetylglucosamine mannoside alpha1-2-acetylglucosaminyltransferase
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UDP-N-acetyl-alpha-D-glucosamine + alpha-D-Man-(1->3)-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-D-Man = UDP + alpha-D-Man-(1->3)-[alpha-D-GlcNAc-(1->2)]-alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-D-Man
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hexosyl group transfer
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UDP-N-acetyl-D-glucosamine:alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->2)-alpha-D-mannosyl-(1->2)-D-mannose alpha-N-acetyl-D-glucosaminyltransferase
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UDP-N-acetyl-alpha-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
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Substrates: -
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UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
additional information
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UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
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Substrates: -
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UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
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Substrates: i.e. mannotetraose acceptor: acceptor substrates are mannoprotein, saccharides with more than 25 mannose-units, with 15 to 25 units, with 8 to 15 units, with 5 to 8 units and below 5 units, the latter being the best substrates
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UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
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Substrates: involved in biosynthesis of mannoprotein side-chain units
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UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
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Substrates: -
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UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
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Substrates: involved in biosynthesis of mannoprotein side-chain units
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additional information
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Substrates: enzyme depends on the UDPase, which generates UDP as antiporter to transport UDP-N-acetyl-D-glucosamine into the Golgi lumen
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additional information
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Substrates: enzyme depends on the UDPase, which generates UDP as antiporter to transport UDP-N-acetyl-D-glucosamine into the Golgi lumen
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UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
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Substrates: involved in biosynthesis of mannoprotein side-chain units
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UDP-N-acetyl-D-glucosamine + 1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
UDP + 1,3-alpha-D-mannosyl-1,2-(N-acetyl-alpha-D-glucosaminyl-alpha-D-mannosyl)-1,2-alpha-D-mannosyl-D-mannose
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Substrates: involved in biosynthesis of mannoprotein side-chain units
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Mn2+
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requirement, 10 mM, inhibits at higher concentrations, Mg2+ or Ca2+ cannot replace Mn2+
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Co2+
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strong, 1 mM, even in the presence of Mn2+, wild-type
Mn2+
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above 10 mM, wild-type
phosphate
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wild-type, complete inhibition at 40 mM
UDP-hexylamine
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wild-type
UDP
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wild-type
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11 - 13
1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
0.0364 - 0.0447
UDP-N-acetylglucosamine
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1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
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solubilized enzyme
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1,3-alpha-D-mannosyl-1,2-alpha-D-mannosyl-1,2-alpha-D-mannosyl-D-mannose
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membrane-bound enzyme
0.0364
UDP-N-acetylglucosamine
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mutant 2-2
0.0447
UDP-N-acetylglucosamine
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wild-type
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additional information
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activity of wild-type and mutant strains with different acceptors
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additional information
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pI: 4.9, in the presence of Triton X-100 and 2 M urea
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yeast, wild-type strains Y-58a his 4c or his 3 and mutant strains mnn1, mnn2-1 or mnn2-2
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integral membrane protein
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catalytic reaction takes place in the lumen of Golgi vesicles
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catalytic reaction takes place in the lumen of Golgi vesicles
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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300000
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gel filtration in the presence of Triton X-100, native MW may be lower
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glycoprotein
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possesses no N-acetylglucosamine residues, enzyme does not bind to wheat germ agglutinin
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additional information
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enzyme activity in KL8 null mutant, which has no remaining UDPase activity, is reduced due to lack of UDP-N-acetyl-D-glucosaminose
additional information
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analysis of mutations in strain mnn2-2
additional information
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enzyme activity in KL8 null mutant, which has no remaining UDPase activity, is reduced due to lack of UDP-N-acetyl-D-glucosaminose
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Triton X-100 stabilizes solubilized enzyme
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additional information
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organic solvent extraction, unstable to
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4°C, in imidazole-glycerol buffer, 2% Triton X-100, t1/2: 80 days
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Douglas, R.H.; Ballou, C.E.
Purification of an alpha-N-acetylglucosaminyltransferase from the yeast Kluyveromyces lactis and a study of mutants defective in this enzyme activity
Biochemistry
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1561-1570
1982
Kluyveromyces lactis
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Lopez-Avalos, M.D.; Uccelletti, D.; Abeijon, C.; Hirschberg, C.B.
The UDPase activity of the Kluyveromyces lactis Golgi GDPase has a role in uridine nucleotide sugar transport into Golgi vesicles
Glycobiology
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413-422
2001
Kluyveromyces lactis, Kluyveromyces lactis KL8
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