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Information on EC 2.3.3.9 - malate synthase and Organism(s) Pseudomonas aeruginosa

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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.9 malate synthase
IUBMB Comments
The enzyme catalyses the irreversible condensation of acetyl-CoA with glyoxylate to form (S)-malate. Among other functions, the enzyme participates in the glyoxylate cycle, a modified version of the TCA cycle that bypasses steps that lead to a loss of CO2.
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Select one or more organisms in this record: ?
This record set is specific for:
Pseudomonas aeruginosa
Word Map
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The enzyme appears in selected viruses and cellular organisms
Synonyms
malate synthase, malate synthase g, glyoxylate cycle enzyme, malate synthetase, malate synthase a, malate synthase 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glyoxylate transacetase
-
-
-
0
glyoxylic transacetase
-
-
-
0
L-malate glyoxylate-lyase (CoA-acetylating)
-
-
-
0
malate synthase 1
-
-
-
0
malate synthase G
malate synthetase
-
-
-
0
malic synthetase
-
-
-
0
malic-condensing enzyme
-
-
-
0
MSG
-
-
-
0
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aldol condensation
-
-
-
0
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:glyoxylate C-acetyltransferase (thioester-hydrolysing, carboxymethyl-forming)
The enzyme catalyses the irreversible condensation of acetyl-CoA with glyoxylate to form (S)-malate. Among other functions, the enzyme participates in the glyoxylate cycle, a modified version of the TCA cycle that bypasses steps that lead to a loss of CO2.
CAS REGISTRY NUMBER
COMMENTARY hide
9013-48-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + glyoxylate + H2O
(S)-malate + CoA
show the reaction diagram
-
-
-
0
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012
acetyl-CoA
-
-
0.07
glyoxylate
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
pyruvate
Pseudomonas aeruginosa
-
1 mM pyruvate: 40% activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
85% activity
7
-
87% activity
additional information
-
below pH 6: no activity remains, outside pH 7-10 the activity decreases rapidly
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15
-
47.1% activity
32.5 - 40
-
more than 90% of activity
5
-
15.5% activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A0C6EL94_PSEAI
725
0
78646
TrEMBL
-
A0A2S5IAA7_PSEAI
725
0
78331
TrEMBL
-
A0A367LYE3_PSEAI
165
0
18202
TrEMBL
-
A0A643ES10_PSEAI
725
0
78646
TrEMBL
-
A0A3E1MT54_PSEAI
108
0
11334
TrEMBL
-
A0A2V3GV48_PSEAI
251
0
27641
TrEMBL
-
A0A5F1BTQ6_PSEAI
725
0
78660
TrEMBL
-
A0A4P0TLV2_PSEAI
684
0
74262
TrEMBL
-
A0A431XHR2_PSEAI
725
0
78577
TrEMBL
-
A0A0C7D2Q3_PSEAI
725
0
78632
TrEMBL
-
A0A4P0TLL6_PSEAI
41
0
4477
TrEMBL
-
A0A2R3J4N5_PSEAI
725
0
78556
TrEMBL
-
A0A233T093_PSEAI
725
0
78644
TrEMBL
-
A0A5R1BQ98_PSEAI
725
0
78659
TrEMBL
-
A0A6B0QHS1_PSEAI
725
0
78662
TrEMBL
-
A0A6H1QC36_PSEAI
725
0
78674
TrEMBL
-
A0A485E8G8_PSEAI
726
0
79237
TrEMBL
-
A0A0A8R9T0_PSEAI
725
0
78618
TrEMBL
-
A0A367ME64_PSEAI
313
0
33316
TrEMBL
-
A0A659BXL1_PSEAI
725
0
78644
TrEMBL
-
A0A485FWH9_PSEAI
725
0
78525
TrEMBL
-
A0A6A9K126_PSEAI
725
0
78601
TrEMBL
-
A0A367M1C9_PSEAI
117
0
13244
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
79400
-
x * 79400, predicted and observed (SDS-PAGE)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 79400, predicted and observed (SDS-PAGE)
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1 - 2
-
1 h preincubation: about 5% activity
702873
11
-
1 h preincubation: 90% activity
702873
5
-
1 h preincubation: about 5% activity
702873
6 - 7
-
1 h preincubation: 100% activity
702873
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
1 h preincubation: 100% activity
55
-
1 h preincubation: 47% activity
70
-
1 h preincubation: complete loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
glutathione affinity chromatography, proteolytic removal of the GST tag, anion exchange chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
GST-tagged version expressed in Escherichia coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Roucourt, B.; Minnebo, N.; Augustijns, P.; Hertveldt, K.; Volckaert, G.; Lavigne, R.
Biochemical characterization of malate synthase G of P. aeruginosa
BMC Biochem.
10
20
2009
Pseudomonas aeruginosa
Manually annotated by BRENDA team
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