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Information on EC 2.3.3.18 - 2-phosphinomethylmalate synthase for references in articles please use BRENDA:EC2.3.3.18
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EC Tree
IUBMB Comments The enzyme, characterized from the bacterium Streptomyces hygroscopicus, participates in the pathway for bialaphos biosynthesis. It requires a divalent metal ion and can also act on oxaloacetate.
The expected taxonomic range for this enzyme is: Streptomyces hygroscopicus
Synonyms
2-phosphinomethylmalic acid synthase, pmmS,
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2-phosphinomethylmalic acid synthase
2-phosphinomethylmalic acid synthase
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2-phosphinomethylmalic acid synthase
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pmmS
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gene name
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acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate = phosphinomethylmalate + CoA
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-
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acetyl-CoA:phosphinopyruvate C-acetyltransferase (thioester-hydrolysing, phosphinomethylmalate-forming)
The enzyme, characterized from the bacterium Streptomyces hygroscopicus, participates in the pathway for bialaphos biosynthesis. It requires a divalent metal ion and can also act on oxaloacetate.
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acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
phosphinomethylmalate + CoA
acetyl-CoA + H2O + oxaloacetic acid
? + CoA
additional information
?
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acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
phosphinomethylmalate + CoA
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?
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
phosphinomethylmalate + CoA
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-
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?
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
phosphinomethylmalate + CoA
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-
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?
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
phosphinomethylmalate + CoA
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?
acetyl-CoA + H2O + oxaloacetic acid
? + CoA
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?
acetyl-CoA + H2O + oxaloacetic acid
? + CoA
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?
additional information
?
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no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate
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-
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additional information
?
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no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate
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-
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acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
phosphinomethylmalate + CoA
acetyl-CoA + H2O + oxaloacetic acid
? + CoA
additional information
?
-
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
phosphinomethylmalate + CoA
-
-
-
-
?
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
phosphinomethylmalate + CoA
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?
acetyl-CoA + H2O + oxaloacetic acid
? + CoA
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-
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?
acetyl-CoA + H2O + oxaloacetic acid
? + CoA
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-
-
-
?
additional information
?
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no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate
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-
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additional information
?
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no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate
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2-oxoglutarate
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1 mM, 18% inhibition
ATP
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1 mM, 35% inhibition
Ca2+
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1 mM, 54% residual activity
Fe2+
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1 mM, 50% residual activity
iodoacetamide
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1 mM, 82% inhibition
iodoacetic acid
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1 mM, 18% residual activity
Mg2+
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1 mM, 44% residual activity
Phosphate buffer
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not inhibitory at 10 mM, 50% loss of activity in 100 mM phosphate buffer
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Zn2+
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1 mM, 24% residual activity
4-chloromercuribenzoate
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0.1 mM, 100% inhibition
4-chloromercuribenzoate
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0.1 mM, 2% residual activity
EDTA
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0.05 mM, 100% inhibition
EDTA
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0.05 mM, almost complete inactivation
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0.36 - 0.385
3-(hydroxyphosphinoyl)pyruvate
0.13
oxaloacetic acid
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pH 8.0, 30°C
0.36
3-(hydroxyphosphinoyl)pyruvate
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pH 8.0, 30°C
0.385
3-(hydroxyphosphinoyl)pyruvate
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pH 8.0, 30°C
0.052
acetyl-CoA
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pH 8.0, 30°C
0.069
acetyl-CoA
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pH 8.0, 30°C
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UniProt
brenda
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UniProt
brenda
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PMMS_STRHY
440
0
49199
Swiss-Prot
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48000
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2 * 48000, SDS-PAGE
90000 - 98000
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gel fitlration
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dimer
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2 * 48000, SDS-PAGE
dimer
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2 * 48000, SDS-PAGE
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4°C, lyophilized powder, 5% loss of activity within 3 months
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Shimotohno, K.; Seto, H.; Imai, S.; Murakami, T.
Purification and characterization of citrate synthase from Streptomyces hygroscopicus SF-1293 and comparison of its properties with those of 2-phosphinomethylmalic acid synthase
Agric. Biol. Chem.
54
463-470
1990
Streptomyces hygroscopicus (Q9LCB4), Streptomyces hygroscopicus, Streptomyces hygroscopicus SF-1293 (Q9LCB4)
brenda
Shimotohno, K.; Seto, H.; ÅŒtake, N.; Imai, S.; Murakami, T.
Studies on the biosynthesis of bialaphos (SF-1293). 8. Purification and characterization of 2-phosphinomethylmalic acid synthase from Streptomyces hygroscopicus SF-1293
J. Antibiot.
41
1057-1065
1988
Streptomyces hygroscopicus (Q9LCB4), Streptomyces hygroscopicus, Streptomyces hygroscopicus SF-1293 (Q9LCB4)
brenda
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2.3.3.18 2-phosphinomethylmalate synthase
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