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IUBMB Comments The enzyme from the bacterium Pseudomonas sp. YGJ3 is composed of three subunits named PhlA, PhlB and PhlC. Production of 2,4-diacetylphloroglucinol, which has antibiotic activity, is strongly inhibited by chloride ions.
Synonyms
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monoacetylphloroglucinol acetyltransferase
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MAPG Atase
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2 2-acetylphloroglucinol = 2,4-diacetylphloroglucinol + phloroglucinol
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2-acetylphloroglucinol C-acetyltransferase
The enzyme from the bacterium Pseudomonas sp. YGJ3 is composed of three subunits named PhlA, PhlB and PhlC. Production of 2,4-diacetylphloroglucinol, which has antibiotic activity, is strongly inhibited by chloride ions.
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2 2-acetylphloroglucinol
2,4-diacetylphloroglucinol + phloroglucinol
Substrates: -
r
2,4-diacetylphloroglucinol + phloroglucinol
2 2-acetylphloroglucinol
Substrates: -
r
2,4-diacetylphloroglucinol + resorcinol
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Substrates: -
r
additional information
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Substrates: no activity with pyrocatechol
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2 2-acetylphloroglucinol
2,4-diacetylphloroglucinol + phloroglucinol
Substrates: -
r
2,4-diacetylphloroglucinol + phloroglucinol
2 2-acetylphloroglucinol
Substrates: -
r
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2,4-diacetylphloroglucinol
2,4-diacetylphloroglucinol inhibits the forward reaction competitively
Cl-
inhibited by 2 mM Cl-
diethylpyrocarbonate
88% inhibition at 1 mM
Hg2+
complete inhibition at 0.1 mM
p-chloromercuribenzoate
complete inhibition at 1 mM
pyoluteorin
inhibited by 0.05 mM pyoluteorin
additional information
NaCl, MgCl2, CaCl2,FeCl3, ZnCl2, CuCl2, and NiCl2, and various metal chelators (0.5 mM), including 1,10-phenanthroline, 8-hydroxyquinoline, 2,2'-dipyridine, and catechol-3,5-disulfonic acid disodium salt, have almost no effect on enzyme activity. Neither activation nor inhibition is found with thiols (1mM) such as L-cysteine, 2-mercaptoethanol, and dithiothreitol; not inhibited by 2 mM Br- or 2 mM I-, as well as phloroglucinol, acetyl-CoA, and malonyl-CoA
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0.019
2,4-diacetylphloroglucinol
at pH 7.0 and 25°C
0.26
2-acetylphloroglucinol
at pH 7.0 and 25°C
1.31
phloroglucinol
at pH 7.0 and 25°C
0.77
resorcinol
at pH 7.0 and 25°C
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0.018
2,4-diacetylphloroglucinol
at pH 7.0 and 25°C
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6.3 - 7.2
the forward and the reverse reactions show optimum pH values at about 6.3-7.2
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subunit PhlB
UniProt
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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F7J5Y3_9PSED
146
0
16644
TrEMBL
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heterotrimer
x * 42500 + x * 38500 + x * 16600, calculated from amino acid sequence
heterotrimer
x * 43000 + x * 39000 + x * 17000, SDS-PAGE
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40 - 65
the enzyme retains its activity below 40°C for 20 min, but is inactivated at above 65°C
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ammonium sulfate precipitation, Q Sepharose column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration
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expressed in Escherichia coli XL1-Blue cells
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2 mM Cl- and 0.05 mM pyoluteorin repress the expression of the enzyme
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Hayashi, A.; Saitou, H.; Mori, T.; Matano, I.; Sugisaki, H.; Maruyama, K.
Molecular and catalytic properties of monoacetylphloroglucinol acetyltransferase from Pseudomonas sp. YGJ3
Biosci. Biotechnol. Biochem.
76
559-566
2012
Pseudomonas sp. YGJ3 (F7J5Y3)
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