This polyketide synthase enzyme forms the (S)-2-methylbutanoate side chain during lovastatin biosynthesis by the filamentous fungus Aspergillus terreus. The overall reaction comprises a single condensation reaction followed by α-methylation, β-ketoreduction, dehydration, and α,β-enoyl reduction.
The enzyme appears in viruses and cellular organisms
This polyketide synthase enzyme forms the (S)-2-methylbutanoate side chain during lovastatin biosynthesis by the filamentous fungus Aspergillus terreus. The overall reaction comprises a single condensation reaction followed by alpha-methylation, beta-ketoreduction, dehydration, and alpha,beta-enoyl reduction.
Substrates: the overall reaction comprises a single condensation reaction followed by alpha-methylation, beta-ketoreduction, dehydration, and alpha,beta enoyl reduction Products: -
enzyme LovF synthesizes the alpha-S-methylbutyrate side chain that is subsequently transferred to monacolin J to yield the cholesterol-lowering natural product lovastatin. The diketide product of LovF is offloaded from the LovF acyl carrier protein domain by the dissociated acyltransferase LovD. LovD primarily interacts with the acyl carrier protein domain of LovF and the protein-protein interactions lead to highly efficient transfer of the diketide product. The catalytic efficiency is enhanced nearly one millionfold when LovF is used as the acyl carrier instead of N-acetylcysteamine
using a multivector expression system in Saccharomyces cerevisiae, two natural statins from two fungal species, i.e., lovastatin from Aspergillus terreus and FR901512 from Xylaria grammica are reconstituted, but also new statin structures by mixing their genes
FT-ICR-MS characterization of intermediates in the biosynthesis of the alpha-methylbutyrate side chain of lovastatin by the 277 kDa polyketide synthase LovF
Bhargavi, S.; Praveen, V.; Anil Kumar, M.; Savitha, J.
Comparative study on whole genome sequences of Aspergillus terreus (soil fungus) and Diaporthe ampelina (endophytic fungus) with reference to lovastatin production
Curr. Microbiol.
75
84-91
2018
Aspergillus terreus, Aspergillus terreus KM017963, no activity in Diaporthe ampelina
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