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Information on EC 2.3.1.23 - 1-acylglycerophosphocholine O-acyltransferase
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EC Tree
2.3.1.23 1-acylglycerophosphocholine O-acyltransferaseIUBMB Comments
Acts preferentially with unsaturated acyl-CoA derivatives. 1-Acyl-sn-glycero-3-phosphoinositol can also act as acceptor.
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Word Map
- 2.3.1.23
- lysophospholipids
- acyl-coas
- microsomal
- phospholipase
-
arachidonic
-
lysophosphatidic
-
reacylation
-
polyunsaturated
-
platelet-activating
- glycerophospholipids
-
cholinephosphotransferase
- arachidonoyl-coa
- oleoyl-coa
- palmitoyl-coa
-
deacylation-reacylation
- lysophospholipase
- glycerolipids
-
lysopcs
- lysophosphatidylinositol
- lysophosphatidylserine
-
kennedy
-
oleoyl
-
acyl-coa-dependent
-
transacylation
-
cholinephosphate
-
1-acyl-gpc
-
arachidonoyl
- thimerosal
- linoleoyl-coa
-
coa-independent
-
mboats
-
disaturated
-
lecithin-cholesterol
-
lpats
- glycerolphosphate
- pharmacology
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
1-acyl-sn-glycero-3-phosphocholine acyltransferase, 1-acylglycerophosphocholine acyltransferase, 1-AGPAT7, acetyl-CoA:1-O-alkyl-sn-glycero-3-phosphocholine acetyltransferase, acyl coenzyme A-monoacylphosphatidylcholine acyltransferase, acyl-CoA:1-acyl-glycero-3-phosphocholine transacylase, acyl-CoA:glycerophosphocholine acyltransferase, acyl-CoA:lysophosphatidylcholine acyltransferase, acyl-CoA:lysophosphatidylethanolamine acyltransferase 2, acyl-CoA:lysophospholipid acyltransferase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1-acyl-sn-glycero-3-phosphocholine acyltransferase
-
-
-
-
1-acylglycerophosphocholine acyltransferase
acyl coenzyme A-monoacylphosphatidylcholine acyltransferase
-
-
-
-
acyl-CoA:1-acyl-glycero-3-phosphocholine transacylase
-
-
-
-
acyl-CoA:lysophosphatidylcholine acyltransferase
acyltransferase, lysolecithin
-
-
-
-
AYTL3
LPAT
LPCAT
LPCAT1
LPCAT2
LPCAT3
LPCAT4
lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1
lyso-PC acyltransferase 1
lysolecithin acyltransferase
-
-
-
-
lysoPC acyltransferase
lysophosphatide acyltransferase
-
-
-
-
lysophosphatidylcholine acyltransferase
lysophosphatidylcholine acyltransferase 1
lysophosphatidylcholine acyltransferase 3
lysophosphocholine acyltransferase 1
lysophosphocholine acyltransferase 2
lysophosphocholine acyltransferase 3
lysophosphocholine acyltransferase 4
lysophospholipid acyltransferase
PGAT
YALI0E16797g
lysophosphatidylcholine acyltransferase
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-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
-
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
lung enzyme displays an iso-ping-pong mechanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
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-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
MetaCyc
arachidonate biosynthesis I (6-desaturase, lower eukaryotes), dimorphecolate biosynthesis, docosahexaenoate biosynthesis I (lower eukaryotes), hydroxylated fatty acid biosynthesis (plants), icosapentaenoate biosynthesis I (lower eukaryotes), linoleate biosynthesis I (plants), phosphatidylcholine acyl editing, phosphatidylcholine biosynthesis VII, phospholipid remodeling (phosphatidylcholine, yeast), phospholipid remodeling (phosphatidylethanolamine, yeast), ricinoleate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:1-acyl-sn-glycero-3-phosphocholine O-acyltransferase
Acts preferentially with unsaturated acyl-CoA derivatives. 1-Acyl-sn-glycero-3-phosphoinositol can also act as acceptor.
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CAS REGISTRY NUMBER
COMMENTARY
9027-64-9
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-acyl-2-lysophosphatidylethanolamine + oleoyl-CoA
1-acyl-2-oleoylphosphatidylethanolamine + CoA
-
-
-
?
1-lauroyl-2-lysophosphatidylcholine + oleoyl-CoA
CoA + 1-lauroyl-2-oleoylphosphatidylcholine
-
-
-
-
?
1-lauroyl-2-lysophosphatidylcholine + palmitoyl-CoA
CoA + 1-lauroyl-2-palmitoylphosphatidylcholine
-
-
-
-
?
1-lauroyl-2-lysophosphatidylcholine + stearoyl-CoA
CoA + 1-lauroyl-2-stearoylphosphatidylcholine
-
-
-
-
?
1-myristoyl-lysophosphatidylcholine + palmitoyl-CoA
CoA + 1-myristoyl-2-palmitoylphosphatidylcholine
-
-
-
-
?
1-oleoyl-2-lysophosphatidylcholine + oleoyl-CoA
CoA + 1,2-dioleoylphosphatidylcholine
-
-
-
-
?
1-oleoyl-2-lysophosphatidylcholine + palmitoyl-CoA
CoA + 1-oleoyl-2-palmitoylphosphatidylcholine
-
-
-
-
?
1-oleoyl-2-lysophosphatidylcholine + stearoyl-CoA
CoA + 1-oleoyl-2-stearoylphosphatidylcholine
-
-
-
-
?
1-oleoyl-lysophosphatidylcholine + palmitoyl-CoA
CoA + 1-oleoyl-2-palmitoylphosphatidylcholine
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + alpha-linolenoyl-CoA
1-palmitoyl-2-alpha-linolenoylphosphatidylcholine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + arachidonoyl-CoA
1-palmitoyl-2-arachidonoylphosphatidylcholine + CoA
1-palmitoyl-2-lysophosphatidylcholine + linolenoyl-CoA
1-palmitoyl-2-alpha-linolenoylphosphatidylcholine + CoA
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + linoleoyl-CoA
1-palmitoyl-2-linoleoylphosphatidylcholine + CoA
1-palmitoyl-2-lysophosphatidylcholine + oleoyl-CoA
1-palmitoyl-2-oleoylphosphatidylcholine + CoA
1-palmitoyl-2-lysophosphatidylcholine + oleoyl-CoA
CoA + 2-oleoyl-1-palmitoylphosphatidylcholine
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + palmitoyl-CoA
1,2-dipalmitoylphosphatidylcholine + CoA
1-palmitoyl-2-lysophosphatidylcholine + palmitoyl-CoA
CoA + 1,2-dipalmitoylphosphatidylcholine
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + stearoyl-CoA
1-palmitoyl-2-stearoylphosphatidylcholine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + stearoyl-CoA
CoA + 1-palmitoyl-2-stearoylphosphatidylcholine
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylethanolamine + arachidonoyl-CoA
1-palmitoyl-2-arachidonoylphosphatidylethanolamine + CoA
-
-
-
?
1-palmitoyl-2-lysophosphatidylglycerol + palmitoyl-CoA
1,2-dipalmitoylphosphatidylglycerol + CoA
-
activity observed only in the presence of phosphatidylcholine (1 mg/ml)
-
-
?
1-palmitoyl-2-lysophosphatidylserine + arachidonoyl-CoA
1-palmitoyl-2-arachidonoylphosphatidylserine + CoA
1-palmitoyl-2-lysophosphatidylserine + linoleoyl-CoA
1-palmitoyl-2-linoleoylphosphatidylserine + CoA
-
-
-
-
?
1-palmitoyl-lysophosphatidylcholine + arachidonoyl-CoA
CoA + 1-palmitoyl-2-arachidonoylphosphatidylcholine
-
-
-
-
?
1-palmitoyl-lysophosphatidylcholine + lauroyl-CoA
CoA + 1-palmitoyl-2-lauroylphosphatidylcholine
-
-
-
-
?
1-palmitoyl-lysophosphatidylcholine + linoleoyl-CoA
CoA + 1-palmitoyl-2-linoleoylphosphatidylcholine
-
-
-
-
?
1-palmitoyl-lysophosphatidylcholine + n-octanoyl-CoA
CoA + 1-palmitoyl-2-octanoylphosphatidylcholine
-
-
-
-
?
1-palmitoyl-lysophosphatidylcholine + oleoyl-CoA
CoA + 2-oleoyl-1-palmitoylphosphatidylcholine
-
-
-
-
?
1-palmitoyl-lysophosphatidylcholine + palmitoyl-CoA
CoA + 1,2-dipalmitoylphosphatidylcholine
-
-
-
-
?
1-palmitoyl-lysophosphatidylcholine + stearoyl-CoA
CoA + 1-palmitoyl-2-stearoylphosphatidylcholine
-
-
-
-
?
1-stearoyl-2-lysophosphatidylcholine + oleoyl-CoA
CoA + 2-oleoyl-1-stearoylphosphatidylcholine
-
-
-
-
?
1-stearoyl-2-lysophosphatidylcholine + palmitoyl-CoA
CoA + 2-palmitoyl-1-stearoylphosphatidylcholine
-
-
-
-
?
1-stearoyl-2-lysophosphatidylcholine + stearoyl-CoA
CoA + 1,2-distearoylphosphatidylcholine
-
-
-
-
?
1-stearoyl-lysophosphatidylcholine + palmitoyl-CoA
CoA + 1-stearoyl-2-palmitoylphosphatidylcholine
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
acyl-CoA + 1-acyl-sn-glycero-3-phosphoryldimethylethanolamine
CoA + 1,2-diacyl-sn-glycero-3-phosphoryldimethylethanolamine
-
preferred acyl acceptors in descending order: 1-acyl-sn-glycero-3-phosphocholine, 1-acyl-sn-glycero-3-phosphoryldimethylethanolamine, 1-acyl-sn-glycero-3-phosphorylmonomethylethanolamine, 1-acyl-sn-glycero-3-phosphorylethanolamine
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphorylethanolamine
CoA + 1,2-diacyl-sn-glycero-3-phosphorylethanolamine
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphorylmonomethylethanolamine
CoA + 1,2-diacyl-sn-glycero-3-phosphorylmonomethylethanolamine
-
-
-
?
acyl-CoA + 1-decanoyl-sn-glycero-3-phosphocholine
CoA + 1-decanoyl-2-acyl-sn-glycero-3-phosphocholine
-
-
-
?
acyl-CoA + 1-lauroyl-sn-glycero-3-phosphocholine
CoA + 1-lauroyl-2-acyl-sn-glycero-3-phosphocholine
-
-
-
?
acyl-CoA + 1-myristoyl-sn-glycero-3-phosphocholine
CoA + 1-myristoyl-2-acyl-sn-glycero-3-phosphocholine
-
preferred acyl acceptors: 1-myristyl-sn-glycero-3-phosphocholine, 1-stearyl-sn-glycero-3-phosphocholine, 1-oleoyl-sn-glycero-3-phosphocholine and 1-linoleoyl-sn-glycero-3-phosphocholine, very little activity with 1-decanoyl-sn-glycero-3-phosphocholine
-
?
acyl-CoA + 1-O-alkyl-sn-glycero-3-phosphocholine
CoA + 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine
-
-
-
?
acyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine
CoA + 1-oleoyl-2-acyl-sn-glycero-3-phosphocholine
acyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine
CoA + 1-palmitoyl-2-acyl-sn-glycero-3-phosphocholine
acyl-CoA + 1-stearoyl-sn-glycero-3-phosphocholine
CoA + 1-stearoyl-2-acyl-sn-glycero-3-phosphocholine
-
no activity with 1-myristoyl-sn-glycero-3-phosphocholine
-
r
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoinositol
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoinositol
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoserine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoserine
arachidonoyl-CoA + 1-arachidoyl-2-lysophosphatidylcholine
CoA + 2-arachidonoyl-1-arachidoylphosphatidylcholine
-
-
-
?
arachidonoyl-CoA + 1-O-hexadecyl-sn-glycero-3-phosphocholine
CoA + 2-arachidonoyl-1-O-hexadecyl-sn-glycero-3-phosphocholine
LPCAT3 acylates 1-O-alkyl-sn-glycero-3-phosphocholine with arachidonyl-CoA but not with acetyl-CoA
-
-
?
arachidonoyl-CoA + 1-palmitoyl-2-lysophosphatidylcholine
CoA + 2-arachidonoyl-1-palmitoylphosphatidylcholine
-
-
-
?
arachidonoyl-CoA + 1-palmitoyl-2-lysophosphatidylethanolamine
CoA + 2-arachidonoyl-1-palmitoylphosphatidylethanolamine
lysophosphatidylethanolamine acylation is evident. But no significant activity of acylation is detected when 16:0-lysophosphatidylglycerol is used as substrate
-
-
?
arachidonoyl-CoA + 1-stearoyl-2-lysophosphatidylcholine
CoA + 2-arachidonoyl-1-stearoylphosphatidylcholine
-
-
-
?
arachidonoyl-CoA + palmitoyl-lysophosphatidylcholine
CoA + 2-arachidonoyl-1-palmitoylphosphatidylcholine
-
-
-
?
cis-11-eicosaenoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-cis-11-eicosaenoyl-sn-glycero-3-phosphocholine
-
13% of activity with arachidonoyl-CoA
-
r
decanoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-decanoyl-sn-glycero-3-phosphocholine
-
58% of activity with arachidonoyl-CoA
-
r
decanoyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine
CoA + 1-palmitoyl-2-decanoyl-sn-glycero-3-phosphocholine
-
highest activity with 1-palmitoyl-sn-glycero-3-phosphocholine as acceptor, preferred acyl donors in descending order: decanoyl-CoA, oleoyl-CoA, arachidonoyl-CoA, stearoyl-CoA, linolenoyl-CoA, dodecanoyl-CoA, palmitoyl-CoA
-
?
eicosatrienoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-eicosatrienoyl-sn-glycero-3-phosphocholine
-
20% of activity with arachidonoyl-CoA
-
r
linolenoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-linolenoyl-sn-glycero-3-phosphocholine
-
8% of activity with arachidonoyl-CoA
-
r
linolenoyl-CoA + 1-linolenoyl-sn-glycero-3-phosphocholine
CoA + 1,2-dilinolenoyl-sn-glycero-3-phosphocholine
-
-
-
-
?
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
linoleoyl-CoA + 1-linoleoyl-sn-glycero-3-phosphocholine
CoA + 1,2-dilinoleoyl-sn-glycero-3-phosphocholine
-
-
-
-
?
linoleoyl-CoA + 1-palmitoyl-2-lysophosphatidylcholine
CoA + 1-palmitoyl-2-linoleoylphosphatidylcholine
-
-
-
-
?
linoleoyl-CoA + 1-palmitoyl-2-lysophosphatidylcholine
CoA + 2-linoleoyl-1-palmitoylphosphatidylcholine
-
-
-
?
lyso-platelet-activating factor + acetyl-CoA
platelet-activating factor + CoA
-
activity observed only in the presence of phosphatidylcholine (1 mg/ml)
-
-
?
myristoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-myristoyl-sn-glycero-3-phosphocholine
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphoinositol
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphoinositol
oleoyl-CoA + 1-azido-oleoyl-sn-lysophosphatidyl-(N-methyl)-choline
CoA + 1-azido-oleoyl-2-oleoyl-sn-lysophosphatidyl-(N-methyl)-choline
-
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine
CoA + 1,2-dioleoyl-sn-glycero-3-phosphocholine
-
-
-
-
?
oleoyl-CoA + 1-palmitoyl-2-lysophosphatidylcholine
CoA + 2-oleoyl-1-palmitoylphosphatidylcholine
-
-
-
?
oleoyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine
CoA + 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
-
-
-
-
?
oleoyl-CoA + lyso-platelet-activating factor
CoA + platelet-activating factor
-
-
-
-
?
oleoyl-CoA + palmitoyl-lysophosphatidylcholine
CoA + 2-oleoyl-1-palmitoylphosphatidylcholine
-
-
-
?
oleoyl-CoA + sn-1-myristoyl-lysophosphatidylcholine
CoA + 1-myristoyl-2-oleoylphosphatidylcholine
-
-
-
-
?
oleoyl-CoA + sn-1-stearoyl-lysophosphatidylcholine
CoA + 2-oleoyl-1-stearoylphosphatidylcholine
-
-
-
-
?
oleyl-CoA + sn-1-oleoyl-lysophosphatidylcholine
CoA + 1,2-dioleoylphosphatidylcholine
-
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-palmitoyl-sn-glycero-3-phosphocholine
palmitoyl-CoA + 1-palmitoyl-2-lysophosphatidylcholine
CoA + 1,2-dipalmitoylphosphatidylcholine
-
-
-
-
?
palmitoyl-CoA + 1-palmitoyl-2-lysophosphatidylcholine
CoA + 2-palmitoyl-1-palmitoylphosphatidylcholine
-
-
-
?
palmitoyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine
CoA + 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
-
preferred substrate
-
-
?
palmitoyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine
CoA + 1,2-dipalmitoylphosphatidylcholine
-
-
-
-
?
palmitoyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine
CoA + dipalmitoylphosphatidylcholine
i.e. 1-palmitoyl-lysophosphatidylcholine
-
-
?
palmitoyl-CoA + sn-1-myristoyl-lysophosphatidylcholine
CoA + 1-myristoyl-2-palmitoylphosphatidylcholine
-
-
-
-
?
palmitoyl-CoA + sn-1-oleoyl-lysophosphatidylcholine
CoA + 1-oleoyl-2-palmitoylphosphatidylcholine
-
-
-
-
?
palmitoyl-CoA + sn-1-stearoyl-lysophosphatidylcholine
CoA + 1-stearoyl-2-palmitoylphosphatidylcholine
-
-
-
-
?
stearoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-stearoyl-sn-glycero-3-phosphocholine
stearoyl-CoA + 1-palmitoyl-2-lysophosphatidylcholine
CoA + 2-stearoyl-1-palmitoylphosphatidylcholine
-
-
-
?
stearoyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine
CoA + 1-palmitoyl-2-stearoyl-sn-glycero-3-phosphocholine
-
-
-
-
?
stearoyl-CoA + 1-stearoyl-sn-glycero-3-phosphocholine
CoA + 1,2-distearoyl-sn-glycero-3-phosphocholine
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + arachidonoyl-CoA
1-palmitoyl-2-arachidonoylphosphatidylcholine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + arachidonoyl-CoA
1-palmitoyl-2-arachidonoylphosphatidylcholine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + arachidonoyl-CoA
1-palmitoyl-2-arachidonoylphosphatidylcholine + CoA
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + linoleoyl-CoA
1-palmitoyl-2-linoleoylphosphatidylcholine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + linoleoyl-CoA
1-palmitoyl-2-linoleoylphosphatidylcholine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + linoleoyl-CoA
1-palmitoyl-2-linoleoylphosphatidylcholine + CoA
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + oleoyl-CoA
1-palmitoyl-2-oleoylphosphatidylcholine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + oleoyl-CoA
1-palmitoyl-2-oleoylphosphatidylcholine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + oleoyl-CoA
1-palmitoyl-2-oleoylphosphatidylcholine + CoA
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + palmitoyl-CoA
1,2-dipalmitoylphosphatidylcholine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + palmitoyl-CoA
1,2-dipalmitoylphosphatidylcholine + CoA
-
activity observed only in the presence of phosphatidylcholine (1 mg/ml)
-
-
?
1-palmitoyl-2-lysophosphatidylserine + arachidonoyl-CoA
1-palmitoyl-2-arachidonoylphosphatidylserine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylserine + arachidonoyl-CoA
1-palmitoyl-2-arachidonoylphosphatidylserine + CoA
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
participates in deacylation/reacylation cycle of cell membranes
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
determines composition of phosphocholine in myocardium
-
-
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
microsomal enzyme is more active toward unsaturated acyl-CoAs, the mitochondrial enzyme has a very high specificity toward linoleoyl-CoA
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
highest activity with polyunsaturated acyl-CoAs e.g. arachidonoyl-CoA, eicosatrienoyl-CoA and linoleoyl-CoA
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
phosphatidylcholine is the major site for polyunsaturated fatty acid synthesis
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
the reverse reaction of enzyme LPCAT uses phosphatidylcholine as substrate and forms acyl-CoA and lysophosphatidylcholine
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
specificity of enzyme LPCAT1 for lyso-PC as an acyl acceptor
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
specificity of enzyme LPCAT1 for lyso-PC as an acyl acceptor
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
2 isoenzymes differing in their selectivity toward saturated and unsaturated fatty acids
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
specificity for acyl-CoA with unsaturated acyl groups, 1-palmitoyl-sn-glycero-3-phosphocholine is a better acceptor than 1-stearyl-sn-glycero-3-phosphocholine
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
highest activity with polyunsaturated acyl-CoAs e.g. arachidonoyl-CoA, eicosatrienoyl-CoA and linoleoyl-CoA
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
selectivity for polyunsaturated fatty acids, preferred acyl donors in descending order: arachidonyl-CoA, eicosapentaenoyl-CoA, docosahexaenoyl-CoA, in platelets, 1-acyl-sn-glycero-3-phosphocholine is a better acceptor than 1-acyl-sn-glycero-3-phosphorylethanolamine
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
participates in deacylation/reacylation cycle of cell membranes
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
r
acyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine
CoA + 1-oleoyl-2-acyl-sn-glycero-3-phosphocholine
-
preferred acyl acceptors in descending order: 1-oleoyl-sn-glycero-3-phosphocholine, 1-myristoyl-sn-glycero-3-phosphocholine, 1-lauroyl-sn-glycero-3-phosphocholine, 1-stearoyl-sn-glycero-3-phosphocholine, 1-palmitoyl-sn-glycero-3-phosphocholine, 1-decanoyl-sn-glycero-3-phosphocholine and 1-arachidonoyl-sn-glycero-3-phosphocholine
-
?
acyl-CoA + 1-oleoyl-sn-glycero-3-phosphocholine
CoA + 1-oleoyl-2-acyl-sn-glycero-3-phosphocholine
-
integral membrane enzyme, preferred acyl acceptors in descending order: 1-oleoyl-sn-glycero-3-phosphocholine, 1-stearoyl-sn-glycero-3-phosphocholine, 1-palmitoyl-sn-glycero-3-phosphocholine, 1-myristoyl-sn-glycero-3-phosphocholine, 1-lauroyl-sn-glycero-3-phosphocholine and 1-decanoyl-sn-glycero-3-phosphocholine
-
?
acyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine
CoA + 1-palmitoyl-2-acyl-sn-glycero-3-phosphocholine
-
no activity with 1-myristoyl-sn-glycero-3-phosphocholine
-
r
acyl-CoA + 1-palmitoyl-sn-glycero-3-phosphocholine
CoA + 1-palmitoyl-2-acyl-sn-glycero-3-phosphocholine
-
at 0.16 mM 1-palmitoyl-sn-glycero-3-phosphocholine is preferred 3.5fold over 1-stearoyl-sn-glycero-3-phosphocholine
-
r
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine
-
preferred acyl donor
-
r
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine
-
preferred acyl donors in descending order: arachidonoyl-CoA, oleoyl-CoA and palmitoyl-CoA
-
-
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine
-
-
-
?
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine
-
arachidonoyl-CoA is the preferred acyl donor at 0.1 mM lysophosphatidylcholine concentrations in neuronal nuclei, 1-palmitoyl-sn-glycero-3-phosphocholine is preferred over 1-oleoyl-sn-glycero-3-phosphocholine as acyl acceptor
-
r
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine
-
preferred acyl donors in descending order: arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA, very little activity with palmitoyl-CoA, lauroyl-CoA, myristoyl-CoA, palmitolenoyl-CoA and stearoyl-CoA
-
r
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine
-
preferred acyl donor
-
r
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine
-
preferred acyl donors in descending order: arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA, palmitoyl-CoA, stearoyl-CoA, 1-palmitoyl-sn-glycero-3-phosphocholine is preferred over 1-stearoyl-sn-glycero-3-phosphocholine as acceptor
-
-
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphocholine
-
peripheral and integral acyltransferase, specific for arachidonoyl-CoA and oleoyl-CoA
-
-
r
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine
-
-
-
?
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoethanolamine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoethanolamine
-
57% and 6% of activity with 1-acyl-sn-glycero-3-phosphocholine in microsomes and phosphatidylcholine vesicles respectively
-
-
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoinositol
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoinositol
-
preferred acyl donor arachidonoyl-CoA, other acyl donors: cis,cis-11,14-eicosadienoyl-CoA, cis-11-eicosaenoyl-CoA, linolenoyl-coA, linoleoyl-CoA, oleoyl-CoA, stearoyl-CoA, palmitoleoyl-CoA, palmitoyl-CoA, dodecanoyl-CoA and decanoyl-coA, no activity with 1-acyl-sn-glycero-3-phosphocholine
-
?
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoinositol
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoinositol
-
-
-
?
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoinositol
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoinositol
-
35% and 8% of activity with 1-acyl-sn-glycero-3-phosphocholine in microsomes and phosphatidylcholine vesicles respectively
-
-
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoserine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoserine
-
-
-
-
-
arachidonoyl-CoA + 1-acyl-sn-glycero-3-phosphoserine
CoA + 1-acyl-2-arachidonoyl-sn-glycero-3-phosphoserine
-
99% and 22% of activity with 1-acyl-sn-glycero-3-phosphocholine in microsomes and phosphatidylcholine vesicles respectively
-
?
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
-
-
r
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
-
-
r
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
-
-
r
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
only acyl donor of the mitochondrial enzyme, 1-acyl-sn-glycero-3-phosphocholine and 1-alkenyl-sn-glycero-3-phosphocholine can act as acyl acceptors
-
r
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
-
-
?
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
-
-
-
-
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
-
-
r
linoleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-linoleoyl-sn-glycero-3-phosphocholine
-
-
-
r
myristoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-myristoyl-sn-glycero-3-phosphocholine
-
-
-
r
myristoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-myristoyl-sn-glycero-3-phosphocholine
-
41% of activity with arachidonoyl-CoA
-
r
myristoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-myristoyl-sn-glycero-3-phosphocholine
-
-
-
r
myristoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-myristoyl-sn-glycero-3-phosphocholine
-
-
-
r
myristoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-myristoyl-sn-glycero-3-phosphocholine
-
-
-
r
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
-
-
r
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
-
-
r
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
best acyl donor
-
r
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
-
-
r
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
-
-
r
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
-
-
r
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
-
-
r
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
-
-
-
-
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
-
-
r
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine
-
-
-
r
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphoinositol
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphoinositol
-
preferred acyl donors in descending order: oleoyl-CoA, palmitoyl-CoA and arachidonoyl-CoA
-
?
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphoinositol
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphoinositol
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycero-3-phosphoinositol
CoA + 1-acyl-2-oleoyl-sn-glycero-3-phosphoinositol
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-palmitoyl-sn-glycero-3-phosphocholine
-
-
-
r
palmitoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-palmitoyl-sn-glycero-3-phosphocholine
-
-
-
r
palmitoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-palmitoyl-sn-glycero-3-phosphocholine
-
-
-
r
palmitoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-palmitoyl-sn-glycero-3-phosphocholine
-
-
-
r
palmitoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-palmitoyl-sn-glycero-3-phosphocholine
-
-
-
r
stearoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-stearoyl-sn-glycero-3-phosphocholine
-
-
-
-
r
stearoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-stearoyl-sn-glycero-3-phosphocholine
-
-
-
-
r
stearoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-stearoyl-sn-glycero-3-phosphocholine
-
-
-
r
stearoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-stearoyl-sn-glycero-3-phosphocholine
-
-
-
-
r
stearoyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1-acyl-2-stearoyl-sn-glycero-3-phosphocholine
-
-
-
-
r
additional information
?
-
-
the specificity of the enzyme may respresent a barrier to the incorporation of medium-chain fatty acids at the sn-2 position of triacylglycerol
-
-
-
additional information
?
-
-
priming of Mono-Mac 6 cells by IFNgamma appears to involve LPCAT
-
-
-
additional information
?
-
-
the enzyme may mediate the priming reactions of monocytes to the cytokine interferon-gamma. The enzyme might also effect the responses of monocytes to the bacterial agent lipopolysaccharide that may be important in the development of sepsis
-
-
-
additional information
?
-
-
low activity with lauroyl-CoA, linoleoyl-CoA and arachidonyl-CoA
-
-
-
additional information
?
-
-
inhibition of Golgi complex-associated LPAT activity by the drug CI-976 stimulates Golgi tubule formation and subsequent redistribution of resident Golgi proteins to the endoplasmic reticulum, affecting secretory and endocytic membrane trafficking pathways, overview
-
-
-
additional information
?
-
-
reduced activity with lysophosphatidylethanolamine and lysophosphatidylserine
-
-
-
additional information
?
-
-
the enzyme is involved in dipalmitoylphosphatidylcholine synthesis
-
-
-
additional information
?
-
the enzyme synthesizes phosphatidylcholine in pulmonary surfactant and plays a pivotal role in respiratory physiology and in membrane biogenesis
-
-
-
additional information
?
-
LPCAT1 shows a clear preference for saturated fatty acyl-CoAs, and 1-myristoyl- or 1-palmitoyl-LPC as acyl donors and acceptors, respectively
-
-
-
additional information
?
-
-
substrate specificity, LPCAT prefers lysophosphatidylcholine as a substrate over lysophosphatidic acid, lysophosphatidylinositol, lysophosphatidylserine, lysophosphatidylethanolamine, or lysophosphatidylglycerol, and prefers palmitoyl-CoA to oleoyl-CoA as the acyl donor, the enzyme is involved in synthesis of membrane surfactant proteins and regulation of surfactant phospholipid biosynthesis
-
-
-
additional information
?
-
-
histone H4 protein is subject to palmitoylation catalyzed by Lpcat1 in a calcium-regulated manner. Cytosolic Lpcat1 shifts into the nucleus in lung epithelia in response to exogenous Ca2+. Nuclear Lpcat1 colocalizes with and binds to histone H4, where it catalyzes histone H4 palmitoylation. Residue Ser47 within histone H4 serves as a putative acceptor site, indicative of Lpcat1-mediated O-palmitoylation. Lpcat1 knock-down or expression of a histone H4 Ser47A mutant protein in cells decreases cellular mRNA synthesis
-
-
-
additional information
?
-
-
enzyme LPCAT1 directly interacts with StarD10 protein, amino acids 79-271 of LPCAT1 and the steroidogenic acute regulatory protein-related lipid transfer (START) domain of START domain-containing protein 10 (StarD10) are sufficient for this interaction. The enzyme also interacts with StarD7-I but not StarD7-II or StarD2/PCTP transfer protein
-
-
-
additional information
?
-
enzyme LPCAT1 directly interacts with StarD10 protein, amino acids 79-271 of LPCAT1 and the steroidogenic acute regulatory protein-related lipid transfer (START) domain of START domain-containing protein 10 (StarD10) are sufficient for this interaction. The enzyme also interacts with StarD7-I but not StarD7-II or StarD2/PCTP transfer protein
-
-
-
additional information
?
-
-
Golgi membrane tubule formation can result from increasing the content of lysophospholipids in membranes, either by stimulation of a phospholipase A2 or by inhibition of an lysophosphatidylcholine acyltransferase. The two opposing enzyme activities may help to coordinately regulate Golgi membrane shape and tubule formation
-
-
-
additional information
?
-
-
inhibition of Golgi complex-associated LPAT activity by the drug CI-976 stimulates Golgi tubule formation and subsequent redistribution of resident Golgi proteins to the endoplasmic reticulum, affecting secretory and endocytic membrane trafficking pathways, overview
-
-
-
additional information
?
-
the enzyme is involved in dipalmitoylphosphatidylcholine synthesis
-
-
-
additional information
?
-
substrate specificity, LPCAT prefers lysophosphatidylcholine as a substrate over lysophosphatidic acid, lysophosphatidylinositol, lysophosphatidylserine, lysophosphatidylethanolamine, or lysophosphatidylglycerol, and prefers palmitoyl-CoA to oleoyl-CoA as the acyl donor, the enzyme is involved in synthesis of membrane surfactant proteins and regulation of surfactant phospholipid biosynthesis
-
-
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-palmitoyl-2-lysophosphatidylcholine + arachidonoyl-CoA
1-palmitoyl-2-arachidonoylphosphatidylcholine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + linoleoyl-CoA
1-palmitoyl-2-linoleoylphosphatidylcholine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + oleoyl-CoA
1-palmitoyl-2-oleoylphosphatidylcholine + CoA
-
-
-
-
?
1-palmitoyl-2-lysophosphatidylcholine + palmitoyl-CoA
1,2-dipalmitoylphosphatidylcholine + CoA
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
participates in deacylation/reacylation cycle of cell membranes
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
determines composition of phosphocholine in myocardium
-
-
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
phosphatidylcholine is the major site for polyunsaturated fatty acid synthesis
-
-
r
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine
CoA + 1,2-diacyl-sn-glycero-3-phosphocholine
-
participates in deacylation/reacylation cycle of cell membranes
-
?
additional information
?
-
-
the specificity of the enzyme may respresent a barrier to the incorporation of medium-chain fatty acids at the sn-2 position of triacylglycerol
-
-
-
additional information
?
-
-
priming of Mono-Mac 6 cells by IFNgamma appears to involve LPCAT
-
-
-
additional information
?
-
-
the enzyme may mediate the priming reactions of monocytes to the cytokine interferon-gamma. The enzyme might also effect the responses of monocytes to the bacterial agent lipopolysaccharide that may be important in the development of sepsis
-
-
-
additional information
?
-
-
inhibition of Golgi complex-associated LPAT activity by the drug CI-976 stimulates Golgi tubule formation and subsequent redistribution of resident Golgi proteins to the endoplasmic reticulum, affecting secretory and endocytic membrane trafficking pathways, overview
-
-
-
additional information
?
-
-
the enzyme is involved in dipalmitoylphosphatidylcholine synthesis
-
-
-
additional information
?
-
the enzyme synthesizes phosphatidylcholine in pulmonary surfactant and plays a pivotal role in respiratory physiology and in membrane biogenesis
-
-
-
additional information
?
-
-
histone H4 protein is subject to palmitoylation catalyzed by Lpcat1 in a calcium-regulated manner. Cytosolic Lpcat1 shifts into the nucleus in lung epithelia in response to exogenous Ca2+. Nuclear Lpcat1 colocalizes with and binds to histone H4, where it catalyzes histone H4 palmitoylation. Residue Ser47 within histone H4 serves as a putative acceptor site, indicative of Lpcat1-mediated O-palmitoylation. Lpcat1 knock-down or expression of a histone H4 Ser47A mutant protein in cells decreases cellular mRNA synthesis
-
-
-
additional information
?
-
-
enzyme LPCAT1 directly interacts with StarD10 protein, amino acids 79-271 of LPCAT1 and the steroidogenic acute regulatory protein-related lipid transfer (START) domain of START domain-containing protein 10 (StarD10) are sufficient for this interaction. The enzyme also interacts with StarD7-I but not StarD7-II or StarD2/PCTP transfer protein
-
-
-
additional information
?
-
enzyme LPCAT1 directly interacts with StarD10 protein, amino acids 79-271 of LPCAT1 and the steroidogenic acute regulatory protein-related lipid transfer (START) domain of START domain-containing protein 10 (StarD10) are sufficient for this interaction. The enzyme also interacts with StarD7-I but not StarD7-II or StarD2/PCTP transfer protein
-
-
-
additional information
?
-
-
Golgi membrane tubule formation can result from increasing the content of lysophospholipids in membranes, either by stimulation of a phospholipase A2 or by inhibition of an lysophosphatidylcholine acyltransferase. The two opposing enzyme activities may help to coordinately regulate Golgi membrane shape and tubule formation
-
-
-
additional information
?
-
-
inhibition of Golgi complex-associated LPAT activity by the drug CI-976 stimulates Golgi tubule formation and subsequent redistribution of resident Golgi proteins to the endoplasmic reticulum, affecting secretory and endocytic membrane trafficking pathways, overview
-
-
-
additional information
?
-
the enzyme is involved in dipalmitoylphosphatidylcholine synthesis
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-azido-oleoyl-sn-lysophosphatidylcholine
-
0.2 mM, 50% inactivation after photolysis
18-(4'-azido-2'-hydroxybenzoylamino)-oleic acid
-
0.4 mM; 73% inhibition, reversible inhibition in the dark, irreversible inactivation after photolysis, used as photoaffinity label
Ag+
-
0.000005 mM, almost complete inhibition, noncompetitive, irreversible, probably due to formation of a thiolate
Cu2+
-
0.00001 mM, 50% inhibition, noncompetitive, irreversible, probably due to formation of a thiolate
Hg2+
-
0.00001 mM, 50% inhibition, noncompetitive, irreversible, probably due to formation of a thiolate
lipopolysaccharide
suppresses the enzyme activity of isozyme LPCAT3 toward linoleoyl-CoA and arachidonoyl-CoA in phorbol 12-myristate 13-acetate-treated U-937 cells
Pb2+
-
0.00002 mM, 50% inhibition, noncompetitive, irreversible, probably due to formation of a thiolate
photooxidized phospholipids
-
0.0001-0.01 mM photoxoidized phospholipids preincubated with 0.1 mg microsomal membranes, approx. 45% loss of initial activity
-
CI-976
-
inhibition of Golgi complex-associated LPAT activity by the drug CI-976 stimulates Golgi tubule formation and subsequent redistribution of resident Golgi proteins to the endoplasmic reticulum, it prevents recycling of transferrin receptors, but does not inhibit transferrin uptake, overview
CI-976
-
complete inhibition at 0.05 mM, IC50: 0.015 mM, inhibition of Golgi complex-associated LPAT activity by the drug CI-976 stimulates Golgi tubule formation and subsequent redistribution of resident Golgi proteins to the endoplasmic reticulum, overview
Cyclic nucleotide phosphodiesterase inhibitors
-
gastric mucosa microsomes
-
N-ethylmaleimide
-
15 min preincubation on ice with 40 mM: activity completely abolished
N-ethylmaleimide
-
1 mM, 30% inhibition after 5 min, 95% inhibition after 45 min
p-chloromercuriphenyl sulfonic acid
-
partially restorable by dithitothreitol
palmitoyl-CoA
-
inhibition at higher concentrations of palmitoyl-CoA, possibly due to a detergent effect
additional information
-
enzyme activity can be inhibited by high levels of acyl acceptors
-
additional information
-
activity is reduced in rigid membranes obtained by incorporation of sphingomyelin and dipalmitoylphosphatidylcholine
-
additional information
-
not inhibited by 2-methoxy-5-methyl-1,3,4-triacetoxybenzene and 1,4-diacetoxy-2-methoxy-5-methyl-3-tetradecanoyloxybenzene
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ca2+-ionophore A23187
-
0.3 units/ml, approx. 5fold activation in endothelial cells in the presence of low-density lipoprotein
dioleoylphosphatidylcholine
-
incorporation in membrane elevates acyltransferase activity
egg yolk phosphatidylcholine
-
incorporation in membrane elevates acyltransferase activity
-
low-density lipoprotein
-
1 mg/ml, approx. 10fold activation in endothelial cells, 7fold activation in the presence of both ionomycin and 1-oleoyl-2-acetyl glycerol
-
phosphatidylethanolamine
-
incorporation in membrane elevates acyltransferase activity
phosphatidylserine
-
incorporation in membrane elevates acyltransferase activity
thrombin
-
0.3 units/ml, approx. 5fold activation in endothelial cells in the presence of low-density lipoprotein
-
phosphatidylinositol
-
incorporation in membrane elevates acyltransferase activity
additional information
-
glucocorticoids and fibroblast growth factor induce the enzyme
-
additional information
-
lipopolysaccharide-induced phosphorylation at residue S34 leads to activation of enzyme. Phosphorylation is triggered through lipopolysaccharide-Toll like receptor 4 signalling and may directly depend on MAPK-activated protein kinase2
-
additional information
glucocorticoids and fibroblast growth factor induce the enzyme
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.005
Arachidonoyl-CoA
-
Vmax: 12.4 pmol/min/microgram protein for arachidonoyl-CoA
additional information
additional information
-
kinetic data of soluble and liposome-bound enzyme
-
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.02
2-methoxy-5-methyl-3-tetradecanoyloxy-1,4-benzoquinone
Rattus norvegicus
-
at pH 6.9 and 37°C
0.003
3-decanoyloxy-2-methoxy-5-methyl-1,4-benzoquinone
Rattus norvegicus
-
at pH 6.9 and 37°C
0.139
3-hexanoyloxy-2-methoxy-5-methyl-1,4-benzoquinone
Rattus norvegicus
-
at pH 6.9 and 37°C
0.015
CI-976
Rattus norvegicus
-
complete inhibition at 0.05 mM, IC50: 0.015 mM, inhibition of Golgi complex-associated LPAT activity by the drug CI-976 stimulates Golgi tubule formation and subsequent redistribution of resident Golgi proteins to the endoplasmic reticulum, overview
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0001
-
activity in freshly prepared erythrocyte membranes with palmitoyl-CoA as acyl donor
0.0006
-
activity in stored erythrocyte membranes with palmitoyl-CoA as acyl donor
0.0007
-
activity in freshly prepared erythrocyte membranes with oleoyl-CoA as acyl donor
0.01
-
partially purified enzyme, 1-palmitoyl-glycero-3-phosphocholine as substrate
0.042
-
integral membrane enzyme, 1-palmitoyl-glycero-3-phosphocholine as substrate
0.268
-
substrate: alpha-linolenoyl-CoA, 28°C, no cooperativity: Hill number 1.0
additional information
additional information
-
among all the different substrates examined, 1-myristoyl-LPC (14:0) and 1-palmitoyl-LPC (16:0) has the most robust activity, whereas no detectable activity is observed using 1-hexanoyl (6:0) and 1-arachidoyl (20:0) LPC as substrates; among the saturated fatty acyl-CoAs, only lauroyl-CoA has comparable activity compared with unsaturated fatty acyl-CoAs. Oleoyl-CoA (18:1), linoleoyl-CoA (18:2), and arachidonoyl-CoA (20:4) all demonstrate robust LPCAT activity
additional information
-
LPCAT3 exhibits the highest activity with lysophosphatidylcholine. LPCAT3 exhibits substrate preferences in the order of 1-stearoyl-2-lysophosphatidylcholine > 1-arachidoyl-2-lysophosphatidylcholine > 1-O-hexadecyl-sn-glycero-3-phosphocholine > 1-palmitoyl-2-lysophosphatidylcholine; LPCAT3 is most active in utilizing linoleoyl-CoA as acyl donor. Order range: linoleoyl-CoA > palmitoyl-CoA > arachidonoyl-CoA > oleoyl-CoA > stearoyl-CoA
additional information
-
activity is different in either its microsomal environment or vesicles and is affected by the phospholipid molecular species environment
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 8
7.4
7
-
1-acyl-sn-glycero-3-phosphocholine and 1-alkenyl-sn-glycero-3-phosphocholine acyltransferase activities
7
-
transfer of arachidonoyl-CoA is more rapid at a higher pH, liver enzyme; transfer of oleoyl-CoA
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 10
-
pH 6.0: about 55% of maximal activity, pH 10.0: about 80% of maximal activity, Golgi-associated enzyme
6 - 9
6.5 - 8
-
pH 6.5: about 80% of maximal activity, pH 8.0: about 70% of maximal activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20
25
30
37
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
high expression of both isoforms LCAT1 and LCAT2; high expression of both isoforms LCAT1 and LCAT2
brenda
-
priming of Mono-Mac-6 cells by IFNgamma appears to involve LPCAT
brenda
additional information
-
determined by RT-PCR analysis. Furthermore, peroxisome proliferator-activated receptor alpha agonists dose-dependently regulate LPCAT3 in liver in a peroxisome proliferator-activated receptor alpha-dependent fashion
brenda
-
-
486486, 486612, 486616, 486618, 486621, 486630, 486633, 486634, 486635, 486636, 486637, 486639, 486640, 486641, 486643, 486648, 657761, 659908, 675007, 706096
brenda
-
LPCAT1 is predominantly expressed in the lung, in particular in alveolar type II cells, but occurs also in other tissues
brenda
LPCAT is predominantly expressed in the lung, LPCAT expression in the developing and adult lung, overview
brenda
additional information
-
LPCAT1 shows a wide tissue distribution, overview
brenda
additional information
expression tissue distribution, overview
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
in addition to endoplasmic reticulum, both isoforms LCAT1 and LCAT2 localize to lipid droplets, which consist of a core of neutral lipids surrounded by a monolayer of phospholipid, mainly phosphatidylcholine. This dual localization is enabled by the monotopic topology of the enzymes with both termini facing the cytoplasm, irrespective of supplementation with oleic acid. Lipid droplets have the ability to locally synthesize phosphatidylcholine and this activity correlates with the LPCAT1 and -2 expression levels; in addition to endoplasmic reticulum, both isoforms LCAT1 and LCAT2 localize to lipid droplets, which consist of a core of neutral lipids surrounded by a monolayer of phospholipid, mainly phosphatidylcholine. This dual localization is enabled by the monotopic topology of the enzymes with both termini facing the cytoplasm, irrespective of supplementation with oleic acid. Lipid droplets have the ability to locally synthesize phosphatidylcholine and this activity correlates with the LPCAT1 and -2 expression levels
brenda
-
associated with, catalytic domain of the enzyme is exposed to the cytosol rather than to lumen of the Golgi cisternae
brenda
-
Golgi membrane tubule formation can result from increasing the content of lysophospholipids in membranes, either by stimulation of a phospholipase A2 or by inhibition of an lysophosphatidylcholine acyltransferase. The two opposing enzyme activities may help to coordinately regulate Golgi membrane shape and tubule formation
brenda
-
2 distinct enzymes: a peripheral and a CHAPS insoluble integral membrane enzyme
brenda
-
membrane-bound, at least a sizable portion of the active center of the enzyme is embedded in the membrane interior
-
brenda
-
membrane-bound, at least a sizable portion of the active center of the enzyme is embedded in the membrane interior
-
-
brenda
-
-
-
486486, 486612, 486616, 486618, 486621, 486630, 486632, 486633, 486634, 486635, 486636, 486637, 486640, 486643, 486645, 486648, 706096, 735644
brenda
-
membrane-bound, at least a sizable portion of the active center of the enzyme is embedded in the membrane interior
-
brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
-
the LPCAT genes are highly conserved, suggesting that the duplicated LPCAT genes were derived from a segmental duplication event. The segmental duplication is the result of a recent whole-genome duplication event in flax
malfunction
metabolism
physiological function
additional information
-
the specific activity of flax LPCAT1-catalyzed forward reaction is about 1000 times higher than that of flax DGAT1-catalyzed reaction when the coexpressed recombinant enzymes are assessed in yeast microsomes under in vitro enzyme assays, overview
malfunction
the liver X receptor-mediated effects on arachidonic acid distribution are abolished by LPCAT3 silencing, and a redistribution of arachidonic acid toward the neutral lipid fraction is observed
malfunction
LPCAT3 knockdown induces a spindle-shaped morphology typical of M1-polarized macrophages, increases the secretion of CXCL10, and decreases the levels of CD206 in interleukin-4-activated U-937 cells. Knockdown of LPCAT3 shifts the differentiation of phorbol ester-treated U-937 cells to M1-polarized macrophages. Decrease in the content of phosphatidylcholine containing linoleic acid or arachidonic acid in biological membranes caused by the suppression of LPCAT3 may influencemembrane fluidity, curvature and function
metabolism
the liver X receptor-mediated induction of enzyme LPCAT3 specifically regulates the metabolism of arachidonic acid, the precursor of key inflammatory mediators, such as eicosanoids. Liver X receptor and lysophosphatidylcholine acyltransferase 3 modulate the fatty acid composition of human macrophages, overview
metabolism
-
the enzyme is involved in the Kennedy pathway for triacylglycerol synthesis, biochemical coupling of isozyme LPCAT1 and the diacylglycerol acyltransferase DGAT1-1-catalyzed reactions, overview. Both enzymes are required for synthesis of triacylgycerol
physiological function
double knockdown of both isoforms LPCAT1 and LPCAT2 in A-431 cells leads to strong reduction of lipid-droplet-associated enzymic activity; double knockdown of both isoforms LPCAT1 and LPCAT2 in A-431 cells or single knockdown of isoform LPCAT1 in Hu-H7 cells leads to strong reduction of lipid-droplet-associated enzymic activity. Reduction of LPCAT1 activity in Hu-H7 cells is associated with a morphological alteration in the lipid droplet pool, the mean lipid droplet size increases, although the amount of stored triacylglycerols remains constant
physiological function
-
histone H4 protein is subject to palmitoylation catalyzed by Lpcat1 in a calcium-regulated manner. Cytosolic Lpcat1 shifts into the nucleus in lung epithelia in response to exogenous Ca2+. Nuclear Lpcat1 colocalizes with and binds to histone H4, where it catalyzes histone H4 palmitoylation. Residue Ser47 within histone H4 serves as a putative acceptor site, indicative of Lpcat1-mediated O-palmitoylation. Lpcat1 knock-down or expression of a histone H4 Ser47A mutant protein in cells decrease cellular mRNA synthesis
physiological function
generation of mice bearing a hypomorphic allele of isoform Lpcat1. Newborn Lpcat1 hypomorphic mice show varying perinatal mortality from respiratory failure, with affected animals demonstrating hallmarks of respiratory distress such as atelectasis and hyaline membranes. Lpcat1 mRNA levels are reduced in newborn Lpcat1 hypomorphic mice and directly correlate with saturated phosphatidylcholine content, LPCAT1 activity, and survival. Surfactant isolated from dead Lpcat1 hypomorphic mice fails to reduce minimum surface tension to wild-type levels. Full LPCAT1 activity is required to achieve the levels of saturated phosphatidylcholine essential for the transition to air breathing
physiological function
in a diacylglycerol acyltransferase 1/diacylglycerol acyltransferase 2/phospholipid:diacylglycerol acyltransferase triple mutant the total lipid% dry cell weight as a percentage of the wild-type strain is 13%
physiological function
the enzyme is involved in the Lands cycle, it controls the fatty acid composition at the sn-2 position of glycerophospholipids and the availability of fatty acids, such as arachidonic acid, used for eicosanoid synthesis. LPCAT3 gene expression is regulated by liver X receptor in human primary macrophages and monocytes. Liver X receptor stimulation significantly enhances the lysophospholipid acyltransferase activity of LPCAT3 increasing the arachidonic acid content in the polar lipid fraction, specifically in phosphatidylcholines. Preconditioning of human macrophages by LXR agonist treatment increases the release of arachidonate-derived eicosanoids, such as prostaglandin E2 and thromboxane after lipopolysaccharide stimulation, with a significant attenuation by LPCAT3 silencing
physiological function
-
phosphatidylcholine is the major site for polyunsaturated fatty acid synthesis. Enzyme lysophosphatidylcholine acyltransferase can transfer polyunsaturated fatty acids on phosphatidylcholine directly into the acyl-CoA pool, making these polyunsaturated fatty acids available for the diacylglycerol acyltransferase (DGAT)-catalyzed reaction for triacylglycerol production, proposed mechanism, overview
physiological function
-
pulmonary surfactant, a mixture of proteins and phospholipids, plays an important role in facilitating gas exchange by maintaining alveolar stability. Saturated phosphatidylcholine, the major component of surfactant, is synthesized both de novo and by the remodeling of unsaturated phosphatidylcholine by lyso-PC acyltransferase 1 (LPCAT1). After synthesis in the endoplasmic reticulum, saturated phosphatidylcholine is routed to lamellar bodies for storage prior to secretion. The enzyme forms a transient complex with saturated phosphatidylcholine and specific phospholipid transport protein(s) to initiate trafficking of saturated phosphatidylcholine from the endoplasmic reticulum to the lamellar bodies
physiological function
lysophospholipid acyltransferases (LPLATs) regulate the diversification of fatty acid composition in biological membranes. Lysophosphatidylcholine acyltransferases (LPCATs) are members of the LPLATs that play a role in inflammatory responses. LPCAT3 is reported to be a major contributor to increase polyunsaturated fatty acids, including linoleic acid and arachidonic acid, and is associated with inflammatory responses in human primary macrophages, and the enzyme LPCAT3 plays an important role in M1/M2-macrophage polarization, analysis of the underlying mechanism
physiological function
-
pulmonary surfactant, a mixture of proteins and phospholipids, plays an important role in facilitating gas exchange by maintaining alveolar stability. Saturated phosphatidylcholine, the major component of surfactant, is synthesized both de novo and by the remodeling of unsaturated phosphatidylcholine by lyso-PC acyltransferase 1 (LPCAT1). After synthesis in the endoplasmic reticulum, saturated phosphatidylcholine is routed to lamellar bodies for storage prior to secretion. The enzyme forms a transient complex with saturated phosphatidylcholine and specific phospholipid transport protein(s) to initiate trafficking of saturated phosphatidylcholine from the endoplasmic reticulum to the lamellar bodies
-
physiological function
-
in a diacylglycerol acyltransferase 1/diacylglycerol acyltransferase 2/phospholipid:diacylglycerol acyltransferase triple mutant the total lipid% dry cell weight as a percentage of the wild-type strain is 13%
-
Show AA Sequence and Transmembrane Helices (118 entries)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
43000
56000
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
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2 proteins of 54000 Da and 61000 Da after photoaffinity labeling
additional information
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one major protein of 21000 Da after purification of the enzyme from vesicles
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
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lipopolysaccharide-induced phosphorylation at residue S34 leads to activation of enzyme. Phosphorylation is triggered through lipopolysaccharide-Toll like receptor 4 signalling and may directly depend on MAPK-activated protein kinase2
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
M427T
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naturally occuring mutation, does not significantly affect the activity or function
additional information
additional information
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in a human hepatoma Huh7 cells, RNA interference-mediated knockdown of LPCAT3 results in virtually complete loss of membrane LPCAT activity, suggesting that LPCAT3 is primarily responsible for hepatic LPCAT activity; membranes from HEK293 cells overexpressing LPCAT3 show significantly increased LPCAT activity as assessed by thin layer chromatography analysis with substrate preference toward unsaturated fatty acids
additional information
LPCAT3 isozyme knockdown to about 25% activity in U-937 cells by siRNA. LPCAT3 siRNA-transfected cells show a spindle-shaped. morphology similar to that of M1-polarized macrophages, whereas control siRNAtransfected cells show a rounded morphology typical ofM2-polarized macrophages. Knockdown of LPCAT3 in phorbol ester-treated U-937 cells decreases LPCAT activity toward linoleoyl-CoA and arachidonoyl-CoA
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
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oleoyl-CoA specific activity loses 50% of activity after 90 min, arachidonoyl-CoA specific activity loses 45% of activity after 90 min
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Cibacron blue agarose, DEAE cellulose, Matrex green A, 1-acylglycerophosphoinositol O-acyltransferase; solubilized with CHAPS
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copurification of His-tagged or GST-tagged enzyme with His-tagged StarD10 protein from Escherichia coli by affinity chromatography
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partially purified
solubilization and selective extraction with lysolecithin analogs at low concentration
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solubilized by detergent effect of substrates lysolecithin and oleoyl-CoA, ratio 1:1
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solubilized with lysolecithin, reactive Blue-2-agarose, DEAE cellulose, Matrex Green A
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Spharose 6B, Mono Q, severe loss of activity is partially avoided by incorporation of 20% glycerol in the elution buffer
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli; expressed in Escherichia coli; expressed in Escherichia coli
expression in A-431 cell and COS-7 cell; expression in A-431 cell, COS-7 cell, and Hu-H7 cell
FLAG-tagged version expressed in CHO-K1 cell; FLAG-tagged version expressed in CHO-K1 cell
FLAG-tagged version expressed in HEK293T cell, expressed in Escherichia coli BL21 (DE3)
gene LPCAT1, recombinant expression of FLAG- and HA-tagged full-length and truncated enzyme in HEK-293 cell microsomes, recombinant coexpression of His-tagged or GST-tagged enzyme with His-tagged StarD10 protein in Escherichia coli
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