Information on EC 2.3.1.225 - protein S-acyltransferase and Organism(s) Homo sapiens

for references in articles please use BRENDA:EC2.3.1.225
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Homo sapiens


The taxonomic range for the selected organisms is: Homo sapiens

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.225
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RECOMMENDED NAME
GeneOntology No.
protein S-acyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
two-step transfer mechanism, ping-pong kinetic mechanism
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SYSTEMATIC NAME
IUBMB Comments
palmitoyl-CoA:[protein]-L-cysteine S-palmitoyltransferase
The enzyme catalyses the posttranslational protein palmitoylation that plays a role in protein-membrane interactions, protein trafficking, and enzyme activity. Palmitoylation increases the hydrophobicity of proteins or protein domains and contributes to their membrane association.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
palmitoyl-CoA + [endothelial nitric oxide synthase]-L-cysteine
[endothelial nitric oxide synthase]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
-
isozyme DHHC-21
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-
r
palmitoyl-CoA + [Ga protein]-L-cysteine
[Ga protein]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
substrate of DHHC3 and DHHC7
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-
r
palmitoyl-CoA + [GAD65]-L-cysteine
[GAD65]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
-
-
-
r
palmitoyl-CoA + [GAP-43 protein]-L-cysteine
[GAP-43 protein]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
substrate of DHHC7 and DHHC15
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-
r
palmitoyl-CoA + [htt(1-548)]-L-cysteine
[htt(1-548)]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
N-terminal fragment of htt(1-548)
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-
r
palmitoyl-CoA + [Lck]-L-cysteine
[Lck]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
nonreceptor tyrosine kinase
-
-
r
palmitoyl-CoA + [N-myristoylated G-protein alphai1]-L-cysteine
[N-myristoylated G-protein alphai1]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
-
-
-
-
r
palmitoyl-CoA + [N-myristoylated Gly-Cys-Gly tripeptide]-L-cysteine
[N-myristoylated Gly-Cys-Gly tripeptide]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
-
peptide substrate si tagged via ethylenediamine with fluorescent NBD
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-
r
palmitoyl-CoA + [protein]-L-cysteine
[protein]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
palmitoyl-CoA + [PSD-95]-L-cysteine
[PSD-95]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
low activity
-
-
r
palmitoyl-CoA + [PSD95]-L-cysteine
[Ras]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
palmitoyl-CoA + [Ras]-L-cysteine
[Ras]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
by Ras PAT containing the DHHC9 protein subunit
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-
r
palmitoyl-CoA + [SNAP-25]-L-cysteine
[SNAP-25]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
palmitoyl-CoA + [synaptotagmin I ]-L-cysteine
[synaptotagmin I ]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
-
-
-
r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
palmitoyl-CoA + [endothelial nitric oxide synthase]-L-cysteine
[endothelial nitric oxide synthase]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
-
isozyme DHHC-21
-
-
r
palmitoyl-CoA + [Ga protein]-L-cysteine
[Ga protein]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
Q8IUH5, Q9Y397
substrate of DHHC3 and DHHC7
-
-
r
palmitoyl-CoA + [GAP-43 protein]-L-cysteine
[GAP-43 protein]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
Q8IUH5, Q9Y397
substrate of DHHC7 and DHHC15
-
-
r
palmitoyl-CoA + [Lck]-L-cysteine
[Lck]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
Q8IUH5, Q9Y397
nonreceptor tyrosine kinase
-
-
r
palmitoyl-CoA + [protein]-L-cysteine
[protein]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
palmitoyl-CoA + [PSD95]-L-cysteine
[Ras]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
Q8IUH5, Q9Y397
possible substrate of DHHC15 and, to a lesser extent, DHHC2, DHHC3, and DHHC7
-
-
r
palmitoyl-CoA + [Ras]-L-cysteine
[Ras]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
Q8IUH5, Q9Y397
by Ras PAT containing the DHHC9 protein subunit
-
-
r
palmitoyl-CoA + [SNAP-25]-L-cysteine
[SNAP-25]-S-palmitoyl-L-cysteine + CoA
show the reaction diagram
Q8IUH5, Q9Y397
substrate of DHHC3 and DHHC7
-
-
r
additional information
?
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in the absence of cellular factors, palmitoyl-CoA is capable of spontaneously S-acylating cysteinyl thiols, overview. G protein alpha subunit GsR is first acylated at Cys-3, then the palmitate is transferred to the amino group of Gly-2 through a cyclic intermediate as is postulated for hedgehog
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-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Bromopalmitate
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additional information
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acyl-CoAs of 14 carbons and longer inhibit palmitoyl-CoA labeling of both substrate and enzyme
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0052
palmitoyl-CoA
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pH 7.4, 25°C, recombinant DHHC3
0.00084
[N-myristoylated Gly-Cys-Gly tripeptide]-L-cysteine
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pH 7.4, 25°C, recombinant DHHC3
additional information
additional information
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Michaelis-Menten kinetics
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
expression of 5 PAT isozymes
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
HIP14 resides on the Golgi and can be observed on cytoplasmic vesicles
Manually annotated by BRENDA team
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enzyme class II
Manually annotated by BRENDA team
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enzyme class I
Manually annotated by BRENDA team
associated; associated; associated
Manually annotated by BRENDA team
additional information
PDB
SCOP
CATH
UNIPROT
ORGANISM
Homo sapiens;
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant FLAG- and His6-tagged DHHC2 from Sf9 cells by nickel and FLAG affinity chromatography to near homogeneity
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
COS-7 cells are cotransfected with the cDNAs for eNOS and HA-tagged DHHC-21
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DHHC16, cloning of cDNA from Jurkat cells, insertion into pmCFP via pENTR/D-TOPO, and expression in T cells deficient in Lck, the T lymphocyte-specific Src family kinase, transient expression of cyan fluorescent protein CFP-tagged DHHC16 in HEK293Acells; DHHC2, cloning of cDNA from Jurkat cells, insertion into pmCFP via pENTR/D-TOPO, and expression in T cells deficient in Lck, the T lymphocyte-specific Src family kinase, transient expression of cyan fluorescent protein CFP-tagged DHHC2 in HEK293Acells; DHHC4, cloning of cDNA from Jurkat cells, insertion into pmCFP via pENTR/D-TOPO, and expression in T cells deficient in Lck, the T lymphocyte-specific Src family kinase, transient expression of cyan fluorescent protein CFP-tagged DHHC4 in HEK293A cells, quantitative expression analysis
expression of FLAG- and His6-tagged DHHC2 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system
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expression of human DHHC9 in Saccharomyces cerevisiae fails to complement an erf2DELTA strain
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C159S
the DHHC15 mutant shows reduced activity compared to the wild-type
additional information