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IUBMB Comments The enzyme is resonsible for the production of (3Z )-hex-3-en-1-yl acetate, the major volatile compound released upon mechanical wounding of the leaves of Arabidopsis thaliana .
Synonyms
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acetyl CoA:(Z)-3-hexen-1-ol acetyltransferase
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acetyl-CoA + (3Z)-hex-3-en-1-ol = CoA + (3Z)-hex-3-en-1-yl acetate
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MetaCyc
traumatin and (Z)-3-hexen-1-yl acetate biosynthesis
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acetyl-CoA:(3Z)-hex-3-en-ol acetyltransferase
The enzyme is resonsible for the production of (3Z)-hex-3-en-1-yl acetate, the major volatile compound released upon mechanical wounding of the leaves of Arabidopsis thaliana [1].
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acetyl-CoA + (3Z)-hex-3-en-ol
(3Z)-hex-3-en-1-yl acetate + CoA
acetyl-CoA + (3Z)-hex-3-en-ol
(Z)-3-hexen-1-yl acetate + coenzyme A
Substrates: -
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acetyl-CoA + 1-octanol
octanyl acetate + CoA
Substrates: the enzyme has the ability to accept several medium-chain-length aliphatic and benzyl-derived alcohols, but has highest catalytic efficiency with (Z)-3-hexen-1-ol. The catalytic efficiency with 1-octanol is 0.2% of the catalytic efficiency with (Z)-3-hexen-1-ol
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additional information
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acetyl-CoA + (3Z)-hex-3-en-ol
(3Z)-hex-3-en-1-yl acetate + CoA
Substrates: -
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acetyl-CoA + (3Z)-hex-3-en-ol
(3Z)-hex-3-en-1-yl acetate + CoA
Substrates: the major volatile released upon mechanical wounding of the leaves is (Z)-3-hexen-1-yl acetate
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acetyl-CoA + (3Z)-hex-3-en-ol
(3Z)-hex-3-en-1-yl acetate + CoA
Substrates: the enzyme has the ability to accept several medium-chain-length aliphatic and benzyl-derived alcohols, but has highest catalytic efficiency with (Z)-3-hexen-1-ol. The catalytic efficiency with 1-octanol is 0.2% of the catalytic efficiency with (Z)-3-hexen-1-ol
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additional information
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Substrates: no activity with malonyl CoA and benzoyl CoA
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additional information
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Substrates: no activity with malonyl CoA and benzoyl CoA
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acetyl-CoA + (3Z)-hex-3-en-ol
(3Z)-hex-3-en-1-yl acetate + CoA
Substrates: the major volatile released upon mechanical wounding of the leaves is (Z)-3-hexen-1-yl acetate
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acetyl-CoA + (3Z)-hex-3-en-ol
(Z)-3-hexen-1-yl acetate + coenzyme A
Substrates: -
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Ca2+
5 mM, strong inhibition
Co2+
5 mM, strong inhibition
Cu2+
5 mM, strong inhibition
Mg2+
5 mM, strong inhibition
Zn2+
5 mM, strong inhibition
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0.126 - 0.251
(3Z)-hex-3-en-ol
0.174
1-Octanol
pH 7.7, 25°C, recombinant His6-tagged enzyme
0.0105 - 0.0385
acetyl-CoA
0.126
(3Z)-hex-3-en-ol
pH 7.7, 25°C, native enzyme, partially purified
0.165
(3Z)-hex-3-en-ol
pH 7.7, 25°C, recombinant His6-tagged enzyme
0.251
(3Z)-hex-3-en-ol
pH 7.7, 25°C, recombinant His6-tagged enzyme
0.0105
acetyl-CoA
pH 7.7, 25°C, recombinant His6-tagged enzyme
0.012
acetyl-CoA
pH 7.7, 25°C, native enzyme, partially purified
0.0385
acetyl-CoA
pH 7.7, 25°C, recombinant His6-tagged enzyme
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11.3
(3Z)-hex-3-en-ol
pH 7.7, 25°C, recombinant His6-tagged enzyme
0.125
1-Octanol
pH 7.7, 25°C, recombinant His6-tagged enzyme
23
acetyl-CoA
pH 7.7, 25°C, recombinant His6-tagged enzyme
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45
(3Z)-hex-3-en-ol
pH 7.7, 25°C, recombinant His6-tagged enzyme
0.072
1-Octanol
pH 7.7, 25°C, recombinant His6-tagged enzyme
597
acetyl-CoA
pH 7.7, 25°C, recombinant His6-tagged enzyme
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6.3 - 9
pH 6.3: 89% of maximal activity, pH 9.0: about 45% of maximal activity
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UniProt
brenda
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enzyme activity in the leaves following wounding increases over time, but this response lagges significantly behind (Z)-3-hexen-1-yl acetate release. A maximum specific activity is reached 12 h after the initial wounding event. CHAT enzyme activity declines by 35% over the course of the next 12 h
brenda
Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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malfunction
RNAi transgenic plants exhibiting a severe reduction in At3g03480 transcript levels emitt no (Z)-3-hexen-1-yl acetate
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CHAT_ARATH
454
0
50290
Swiss-Prot
other Location (Reliability: 4 )
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49000
native plant protein, gel filtration
50290
calculated from sequence
50400
1 * 50400, native plant protein, calculated from sequence
51000
His-tagged protein, gel filtration
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?
x * 50290, calculated from sequence
monomer
1 * 50400, native plant protein, calculated from sequence
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37
30 min, 13% loss of activity
50
30 min, complete loss of activity
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expression in Escherichia coli
expression in Escherichia coli, the coding region of At3g03480 is cloned into a vector that introduces an N-terminal 6His tag
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D'Auria, J.C.; Pichersky, E.; Schaub, A.; Hansel, A.; Gershenzon, J.
Characterization of a BAHD acyltransferase responsible for producing the green leaf volatile (Z)-3-hexen-1-yl acetate in Arabidopsis thaliana
Plant J.
49
194-207
2007
Arabidopsis thaliana (Q9SRQ2), Arabidopsis thaliana
brenda
D'Auria, J.C.; Chen, F.; Pichersky, E.
Characterization of an acyltransferase capable of synthesizing benzyl benzoate and other volatile esters in flowers and damaged leaves of Clarkia breweri
Plant Physiol.
130
466-476
2002
Arabidopsis thaliana (Q9SRQ2)
brenda
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