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Information on EC 2.3.1.186 - pseudotropine acyltransferase
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2.3.1.186 pseudotropine acyltransferaseIUBMB Comments
This enzyme exhibits absolute specificity for the exo/3beta configuration found in pseudotropine as tropine (tropan-3alpha-ol; see EC 2.3.1.185, tropine acyltransferase) and nortropine are not substrates . Acts on a wide range of aliphatic acyl-CoA derivatives, including acetyl-CoA, beta-methylcrotonyl-CoA and tigloyl-CoA .
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The enzyme appears in viruses and cellular organisms
Synonyms
pseudotropine:acyl-coa transferase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetyl-CoA:pseudotropine transferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
an acyl-CoA + pseudotropine = CoA + an O-acylpseudotropine
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:pseudotropine O-acyltransferase
This enzyme exhibits absolute specificity for the exo/3beta configuration found in pseudotropine as tropine (tropan-3alpha-ol; see EC 2.3.1.185, tropine acyltransferase) and nortropine are not substrates [1]. Acts on a wide range of aliphatic acyl-CoA derivatives, including acetyl-CoA, beta-methylcrotonyl-CoA and tigloyl-CoA [1].
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CAS REGISTRY NUMBER
COMMENTARY
138440-78-5
-
162535-26-4
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-methylcrotonyl-CoA + pseudotropine
CoA + O-(3-methylcrotonyl)pseudotropine
-
4% of the activity with tigloyl-CoA
-
-
?
acetyl-CoA + N-(2-fluoroethyl)nortropan-3-ol
CoA + N-(2-fluoroethyl)nortropan-3-ol acetyl ester
-
16% of the activity with acetyl-CoA and pseudotropine
-
-
?
acetyl-CoA + N-ethylnorpseudopelletierin-3-ol
CoA + N-ethylnorpseudopelletierin-3-ol acetyl ester
-
% of the activity with acetyl-CoA and pseudotropine
-
-
?
acetyl-CoA + N-ethylnortropan-3-ol
CoA + N-ethylnortropan-3-ol acetyl ester
-
35% of the activity with acetyl-CoA and pseudotropine
-
-
?
acetyl-CoA + N-iso-propylnorpseudopelletierin-3-ol
CoA + N-iso-propylnorpseudopelletierin-3-ol acetyl ester
-
7% of the activity with acetyl-CoA and pseudotropine
-
-
?
acetyl-CoA + norpseudopelletierin-3-ol
CoA + norpseudopelletierin-3-ol acetyl ester
-
9% of the activity with acetyl-CoA and pseudotropine
-
-
?
acetyl-CoA + pseudopelletierin-3-ol
CoA + pseudopelletierin-3-ol acetyl ester
-
% of the activity with acetyl-CoA and pseudotropine
-
-
?
isobutyryl-CoA + pseudotropine
CoA + O-isobutyrylpseudotropine
-
10% of the activity with tigloyl-CoA
-
-
?
isovaleryl-CoA + pseudotropine
CoA + O-isovalerylpseudotropine
-
2% of the activity with tigloyl-CoA
-
-
?
n-propionyl-CoA + pseudotropine
CoA + O-n-propionylpseudotropine
-
5% of the activity with tigloyl-CoA
-
-
?
tigloyl-CoA + 4-hydroxy-1-methylpiperidine
CoA + O-tigloyl-4-hydroxy-1-methylpiperidine
-
14% of the activity with pseudotropine
-
-
?
tigloyl-CoA + N-(2-fluoroethyl)nortropan-3-ol
CoA + 3-O-tigloyl-N-(2-fluoroethyl)nortropan-3-ol
-
74% of the activity with tigloyl-CoA and pseudotropine
-
-
?
tigloyl-CoA + N-ethylnorpseudopelletierin-3-ol
CoA + 3-O-tigloyl-N-ethylnorpseudopelletierin-3-ol
-
% of the activity with tigloyl-CoA and pseudotropine
-
-
?
tigloyl-CoA + N-ethylnortropan-3-ol
CoA + 3-O-tigloyl-N-ethylnortropan-3-ol
-
81% of the activity with tigloyl-CoA and pseudotropine
-
-
?
tigloyl-CoA + N-iso-propylnorpseudopelletierin-3-ol
CoA + 3-O-tigloyl-N-iso-propylnorpseudopelletierin-3-ol
-
2% of the activity with tigloyl-CoA and pseudotropine
-
-
?
tigloyl-CoA + pseudopelletierin-3-ol
CoA + 3-O-tigloyl-pseudopelletierin-3-ol
-
% of the activity with tigloyl-CoA and pseudotropine
-
-
?
acetyl-CoA + pseudotropine
CoA + O-acetylpseudotropine
-
4% of the activity with tigloyl-CoA
-
-
?
phenylacetyl-CoA + pseudotropine
CoA + O-phenylacetylpseudotropine
-
-
-
-
?
phenylacetyl-CoA + pseudotropine
CoA + O-phenylacetylpseudotropine
-
-
-
-
?
phenylacetyl-CoA + pseudotropine
CoA + O-phenylacetylpseudotropine
-
-
-
-
?
phenylacetyl-CoA + pseudotropine
CoA + O-phenylacetylpseudotropine
-
-
-
-
?
phenylacetyl-CoA + pseudotropine
CoA + O-phenylacetylpseudotropine
-
-
-
-
?
phenylacetyl-CoA + pseudotropine
CoA + O-phenylacetylpseudotropine
-
-
-
-
?
phenylacetyl-CoA + pseudotropine
CoA + O-phenylacetylpseudotropine
-
-
-
-
?
tigloyl-CoA + norpseudopelletierin-3-ol
CoA + 3-O-tigloyl-norpseudopelletierin-3-ol
-
13% of the activity with tigloyl-CoA and pseudotropine
-
-
?
tigloyl-CoA + norpseudopelletierin-3-ol
CoA + 3-O-tigloyl-norpseudopelletierin-3-ol
-
40% of the activity with tigloyl-CoA and pseudotropine
-
-
?
tigloyl-CoA + pseudotropine
CoA + O-tigloylpseudotropine
-
-
-
-
?
additional information
?
-
-
tropine and norpseudotropine are not acylated
-
-
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
9
-
activity is highest in glycine buffer, lowerv activity in Tris and borate buffer
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 10
-
pH 7.0: about30% of maximal activity, pH 10.0: about 60% of maximal activity
7 - 9.5
-
pH 7.0: about 35% of maximal activity, pH 10.0: about 20% of 20% of maximal activity
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
root culture, activity is higher during rapid growth than in stationary phase roots
brenda
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boswell, H.D.; Drger, B.; McLauchlan W.R.; Portsteffen, A.; Robins D.J.; Robins R.J.; Walton, N.J.
Specificities of the enzymes of N-alkyltropane biosynthesis in Brugmansia and Datura
Phytochemistry
52
871-878
1999
Brugmansia x candida
brenda
Robins, R.J.; Bachmann, P.; Robinson, T.; Rhodes, M.J.; Yamada, Y.
The formation of 3alpha- and 3beta-acetoxytropanes by Datura stramonium transformed root cultures involves two acetyl-CoA-dependent acyltransferases
FEBS Lett.
292
293-297
1991
Datura stramonium
brenda
Rabot, S.; Peerless, A.C.J.; Robins, R.J.
Tigloyl-CoA:pseudotropine acyl transferase - an enzyme of tropane alkaloid biosynthesis
Phytochemistry
39
315-322
1995
Datura stramonium
-
brenda
Robins, R.J.; Bachmann, P.; Peerless, A.C.J.; Rabot, S.
Esterification reactions in the biosynthesis of tropane alkaloids in transformed root cultures
Plant Cell Tissue Organ Cult.
38
241-247
1994
Atropa belladonna, Brugmansia x candida, Datura inoxia, Datura stramonium, Hyoscyamus muticus, Datura wrightii, Hyoscyamus muticus H61/21, Datura stramonium cv. D15/5, Datura wrightii Dllll3
-
brenda
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Release 2023.1 (January 2023)
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