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10-deacetylbaccatin III 10-O-acetyltransferase
10-deacetylbaccatin III 10beta-O-acetyltransferase
10-deacetylbaccatin III-10-beta-O-acetyltransferase
10-deacetylbaccatin III-10-O-acetyl transferase
10-deacetylbaccatin III-10-O-acetyltransferase
10-deacetylbaccatin III-10beta-O-acetyltransferase
10-deacetylbaccatin III:10beta-O-acetyltransferase
-
-
10-deacetylbaccatin-III-10beta-O-acetyltransferase
-
10-hydroxytaxane 10-O-acetyltransferase
-
-
-
-
10-hydroxytaxane O-acetyltransferase
-
-
-
-
10beta-O-acetyltransferase
-
-
acetyl CoA:10-deacetylbaccatin-III 10-O-acetyltransferase
-
-
-
-
acetyl coenzyme A:10-hydroxytaxane O-acetyltransferase
-
-
-
-
taxoid 10beta-O-acetyl transferase
-
taxoid 10beta-O-acetyltransferase
-
10-DAB
-
-
10-DBAT
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
10-deacetylbaccatin III 10-O-acetyltransferase
Taxus sp.
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
10-deacetylbaccatin III 10-O-acetyltransferase
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
Taxus sp.
-
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
10-deacetylbaccatin III 10beta-O-acetyltransferase
-
10-deacetylbaccatin III-10-beta-O-acetyltransferase
-
10-deacetylbaccatin III-10-beta-O-acetyltransferase
-
10-deacetylbaccatin III-10-beta-O-acetyltransferase
-
10-deacetylbaccatin III-10-beta-O-acetyltransferase
-
10-deacetylbaccatin III-10-beta-O-acetyltransferase
-
10-deacetylbaccatin III-10-O-acetyl transferase
-
-
10-deacetylbaccatin III-10-O-acetyl transferase
-
-
-
10-deacetylbaccatin III-10-O-acetyl transferase
-
10-deacetylbaccatin III-10-O-acetyltransferase
-
10-deacetylbaccatin III-10-O-acetyltransferase
-
10-deacetylbaccatin III-10-O-acetyltransferase
-
-
10-deacetylbaccatin III-10beta-O-acetyltransferase
-
-
-
-
10-deacetylbaccatin III-10beta-O-acetyltransferase
-
-
DBAT
-
DBAT
gene encoding for 10-deacetylbaccatin-III-10beta-O-acetyltransferase
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acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
acetyl-CoA + 10-deacetyltaxol
CoA + paclitaxel
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetyltaxol
CoA + taxol
acetyl-CoA + 4-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 7,13-diacetyl-4-deacetylbaccatin III
CoA + 13-acetylbaccatin III
-
Substrates: -
Products: -
?
butyryl-CoA + 10-deacetylbaccatin III
CoA + 10-deacetyl-10-butyrylbaccatin III
Taxus sp.
-
Substrates: -
Products: -
?
N-acetyl-D-glucosamine + 10-deacetylbaccatin III
D-glucosamine + baccatin III
Substrates: the yield when using N-acetyl-D-glucosamine as acetyl donor in open-whole-cell catalytic system is 2.13fold of that when using acetyl-CoA. In the in vivo system, the yield increased 24.17%, which may indicate its cooperation with acetyl-CoA
Products: -
?
n-butyryl-CoA + 10-deacetylbaccatin III
CoA + 10-deacetyl-10-butyrylbaccatin III
Taxus sp.
-
Substrates: -
Products: -
?
n-butyryl-CoA + 10-deacetylbaccatin III
CoA + 10-n-butyryl-10-deacetylbaccatin III
Taxus sp.
-
Substrates: -
Products: -
?
propionyl-CoA + 10-deacetylbaccatin III
CoA + 10-deacetyl-10-propionylbaccatin III
Taxus sp.
-
Substrates: -
Products: -
?
propionyl-CoA + 10-deacetylbaccatin III
CoA + 10-propionyl-10-deacetylbaccatin III
Taxus sp.
-
Substrates: -
Products: -
?
vinyl acetate + 10-deacetylbaccatin III
vinyl alcohol + baccatin III
additional information
?
-
Taxus sp.
-
Substrates: DBAT catalyzes the transfer of acetyl, propionyl or n-butyryl from CoA to the C10-hydroxyl of 10-deacetylbaccatin III
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: catalyzes the acetylation of the C10 hydroxyl group of the advanced metabolite 10-deacetylbaccatin III to yield baccatin III, which is the immediate diterpenoid precursor of taxol
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: catalyzes the acetylation of the C10 hydroxyl group of the advanced metabolite 10-deacetylbaccatin III to yield baccatin III, which is the immediate diterpenoid precursor of taxol
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: the enzyme is involved in taxol biosynthesis
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Taxus sp.
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetyltaxol
CoA + taxol
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetyltaxol
CoA + taxol
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetyltaxol
CoA + taxol
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetyltaxol
CoA + taxol
Substrates: -
Products: -
?
vinyl acetate + 10-deacetylbaccatin III
vinyl alcohol + baccatin III
Substrates: -
Products: -
?
vinyl acetate + 10-deacetylbaccatin III
vinyl alcohol + baccatin III
Substrates: 42% of the activity as compared to N-acetyl-D-glucosamine
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
butyryl-CoA + 10-deacetylbaccatin III
CoA + 10-deacetyl-10-butyrylbaccatin III
Taxus sp.
-
Substrates: -
Products: -
?
propionyl-CoA + 10-deacetylbaccatin III
CoA + 10-deacetyl-10-propionylbaccatin III
Taxus sp.
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: the enzyme is involved in taxol biosynthesis
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Taxus sp.
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
-
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
acetyl-CoA + 10-deacetylbaccatin III
CoA + baccatin III
Substrates: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.01 - 3.21
10-deacetylbaccatin III
0.178 - 0.546
10-deacetyltaxol
0.046 - 0.134
vinyl acetate
0.01
10-deacetylbaccatin III
-
0.011
10-deacetylbaccatin III
-
recombinant enzyme with a hexahistidine fusion tag
0.049
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
0.0576
10-deacetylbaccatin III
-
-
0.075
10-deacetylbaccatin III
wild-type enzyme, 30°C, pH not specified in the publication
0.134
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
0.231
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
1.67
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
2.28
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
3.21
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
0.178
10-deacetyltaxol
mutant enzyme F301V, pH 5.5, 37.5°C
0.368
10-deacetyltaxol
mutant enzyme G38R, pH 5.5, 37.5°C
0.424
10-deacetyltaxol
mutant enzyme G38R/F301V, pH 5.5, 37.5°C
0.546
10-deacetyltaxol
wild-type enzyme, pH 5.5, 37.5°
0.008
acetyl-CoA
-
0.0085
acetyl-CoA
-
recombinant enzyme with a hexahistidine fusion tag
0.052
acetyl-CoA
wild-type enzyme, pH 7.2, 37°C
0.073
acetyl-CoA
wild-type enzyme, 30°C, pH not specified in the publication
0.148
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
0.29
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
0.762
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
0.925
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
1.094
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
1.51
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
0.046
vinyl acetate
mutant enzyme I43S/D390R, pH 7.2, 37°C
0.065
vinyl acetate
mutant enzyme D390R, pH 7.2, 37°C
0.065
vinyl acetate
mutant enzyme I43T, pH 7.2, 37°C
0.066
vinyl acetate
mutant enzyme I43S, pH 7.2, 37°C
0.128
vinyl acetate
wild-type enzyme, pH 7.2, 37°C
0.134
vinyl acetate
mutant enzyme D390H, pH 7.2, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.58 - 33.12
10-deacetylbaccatin III
0.000334 - 0.001499
10-deacetyltaxol
0.0101 - 26.65
acetyl-CoA
0.0224 - 0.0263
vinyl acetate
0.58
10-deacetylbaccatin III
-
-
3.59
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
14.69
10-deacetylbaccatin III
wild-type enzyme, 30°C, pH not specified in the publication
21.74
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
27.03
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
27.61
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
33.12
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
0.000334
10-deacetyltaxol
wild-type enzyme, pH 5.5, 37.5°
0.000396
10-deacetyltaxol
mutant enzyme F301V, pH 5.5, 37.5°C
0.000815
10-deacetyltaxol
mutant enzyme G38R, pH 5.5, 37.5°C
0.001499
10-deacetyltaxol
mutant enzyme G38R/F301V, pH 5.5, 37.5°C
0.0101
acetyl-CoA
wild-type enzyme, pH 7.2, 37°C
5.28
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
12.73
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
16.12
acetyl-CoA
wild-type enzyme, 30°C, pH not specified in the publication
18.79
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
20.54
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
26.65
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
0.0224
vinyl acetate
wild-type enzyme, pH 7.2, 37°C
0.0224
vinyl acetate
mutant enzyme D390H, pH 7.2, 37°C
0.0236
vinyl acetate
mutant enzyme I43T, pH 7.2, 37°C
0.0244
vinyl acetate
mutant enzyme I43S, pH 7.2, 37°C
0.0259
vinyl acetate
mutant enzyme D390R, pH 7.2, 37°C
0.0263
vinyl acetate
mutant enzyme I43S/D390R, pH 7.2, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
10 - 200
10-deacetylbaccatin III
0.000611 - 0.003539
10-deacetyltaxol
0.19
4-deacetylbaccatin III
-
-
0.015
7,13-diacetyl-4-deacetylbaccatin III
-
-
0.168 - 0.576
vinyl acetate
10
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
10.07
10-deacetylbaccatin III
-
-
20
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
30
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
70
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
120
10-deacetylbaccatin III
mutant enzyme, 30°C, pH not specified in the publication
200
10-deacetylbaccatin III
wild-type enzyme, 30°C, pH not specified in the publication
0.000611
10-deacetyltaxol
wild-type enzyme, pH 5.5, 37.5°C
0.002218
10-deacetyltaxol
mutant enzyme G38R, pH 5.5, 37.5°C
0.00222
10-deacetyltaxol
mutant enzyme F301V, pH 5.5, 37.5°C
0.003539
10-deacetyltaxol
mutant enzyme G38R/F301V, pH 5.5, 37.5°C
0.193
acetyl-CoA
wild-type enzyme, pH 7.2, 37°C
10
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
20
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
40
acetyl-CoA
mutant enzyme, 30°C, pH not specified in the publication
220
acetyl-CoA
wild-type enzyme, 30°C, pH not specified in the publication
0.168
vinyl acetate
mutant enzyme D390H, pH 7.2, 37°C
0.174
vinyl acetate
wild-type enzyme, pH 7.2, 37°C
0.365
vinyl acetate
mutant enzyme I43T, pH 7.2, 37°C
0.37
vinyl acetate
mutant enzyme I43S, pH 7.2, 37°C
0.4
vinyl acetate
mutant enzyme D390R, pH 7.2, 37°C
0.576
vinyl acetate
mutant enzyme I43S/D390R, pH 7.2, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
F160A
mutation negatively affects activity
F301A
activity against 10-deacetyltaxol is 1.6times more active than wild-type activity, activitys against 10-deacetylbaccatin III is degraded
F301V
mutant enzyme is 2.85times more active against 10-deacetyltaxol than the wild-type enzyme
F400A
mutation negatively affects activity
F44A
activity against 10-deacetyltaxol is nearly undetectable
G359A
mutation negatively affects activity
G361A
no activity against 10-deacetylbaccatin III or 10-deacetyltaxol
G38A
activity against 10-deacetyltaxol is 1.45times more active than wild-type activity, activitys against 10-deacetylbaccatin III is slightly decreased
G38R
mutant enzyme is 2.19times more active against 10-deacetyltaxol than the wild-type enzyme
G38R/F301V
mutant enzyme is 3.7times more active against 10-deacetyltaxol than the wild-type enzyme, activity against 10-deacetylbaccatin III is 69% as compared to wild-type enzyme
H162A
no activity against 10-deacetylbaccatin III or 10-deacetyltaxol
I164A
no activity against 10-deacetylbaccatin III or 10-deacetyltaxol
P37A
mutation negatively affects activity
R363A
no activity against 10-deacetylbaccatin III or 10-deacetyltaxol
D166A
decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA
D34E
decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA
D372E
decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA
D390H
catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is 96% of the wild-type value
D390R
catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 2.3fold
G376A
decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA
H162A
mutation results in complete loss of enzymatic activity, suggesting that the residue histidine at 162 is essential to DBAT activity
I43S
catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 2.1fold
I43S/D390R
catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 3.3fold, compared to that of wild-type enzyme. Catalytic efficiency is 2.99fold higher than the catalytic efficiency of wild-type enzyme towards 10-deacetylbaccatin III and acetyl-CoA
I43T
catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 2.1fold
R363A
decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA
S31A
decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA
additional information
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functional expression in Saccharomyces cerevisiae for reconstruction of taxoid biosynthesis
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expression in Escherichia coli
gene dbat, functional expression in Escherichia coli strain JM109, co-expression with acetyl-CoA synthase, gene acs, EC 6.2.1.1, or with acetoacetate:acetyl-CoA CoA-transferase, genes atoAD of the ato operon, EC 2.8.3.8, expressed from and regulated by genes encoded on the bacterial chromosome, overview
Taxus sp.
-
into the TA cloning vector pMD19-T for sequencing
into the vector pCWori+ for expression in Escherichia coli JM109 and BL21DE3 cells
Taxus sp.
-
overexpression in Escherichia coli. Three kinds of fusion systems with isopropyl-beta-D-1-thiogalactopyranoside inducible promoters are used. The fusion partners are glutathione S-transferase tag, hexahistidine fusion tag, and a biotinylated peptide tag
-
the amplified PCR fragment is subcloned into the pGEM-T Easy vector and subsequently into the pET32a+ vector for expression in Escherichia coli BL21DE3 cells
expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
expression in Escherichia coli
into the TA cloning vector pMD19-T for sequencing
-
into the TA cloning vector pMD19-T for sequencing
-
into the TA cloning vector pMD19-T for sequencing
-
into the TA cloning vector pMD19-T for sequencing
into the TA cloning vector pMD19-T for sequencing
into the TA cloning vector pMD19-T for sequencing
into the TA cloning vector pMD19-T for sequencing
into the TA cloning vector pMD19-T for sequencing
into the TA cloning vector pMD19-T for sequencing
into the TA cloning vector pMD19-T for sequencing
into the TA cloning vector pMD19-T for sequencing
into the TA cloning vector pMD19-T for sequencing
-
into the TA cloning vector pMD19-T for sequencing
into the TA cloning vector pMD19-T for sequencing
-
into the TA cloning vector pMD19-T for sequencing
-
into the TA cloning vector pMD19-T for sequencing
-
into the TA cloning vector pMD19-T for sequencing
-
into the TA cloning vector pMD19-T for sequencing
-
into the TA cloning vector pMD19-T for sequencing
-
into the TA cloning vector pMD19-T for sequencing
-
into the TA cloning vector pMD19-T for sequencing
into the TA cloning vector pMD19-T for sequencing
into the TA cloning vector pMD19-T for sequencing
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biotechnology
Taxus sp.
-
optimizing the semi-biosynthetic method in bacteria potentially provides a practical means of developing commercial-scale production of baccatin III analogs, which serve as key intermediates in the semi-synthesis of second-generation taxols
pharmacology
use in synthesis of taxol for anticnacer treatment
pharmacology
key enzyme in the biosynthesis of the anticancer drug paclitaxel, which catalyses the formation of baccatin III from 10-deacetylbaccatin III
pharmacology
paclitaxel is a type of broad-spectrum anticancer drug in short supply. The price of acetyl-CoA, which is the acetyl group donor for the enzymatic synthesis of the intermediate, baccatin III, is the bottleneck of the mass production of paclitaxel. The study reports that N-acetyl-D-glucosamineas an acetyl group donor can substantially reduce the cost of production
synthesis
Taxus sp.
-
bioengineered Escherichia coli cells synthesize nonnatural 10-deacetyl-10-acylbaccatin III derivatives as potential precursors of second-generation Taxol derivatives using the enzyme in concert with acyl-CoA transferases, determination of optimal alkanoic acid concentration for in vivo production of baccatin III analogues, overview
synthesis
Taxus sp.
-
synthesis of baccatin III in Escherichia coli producing endogenous acetyl-CoA and overexpressing recombinant 10-deacetylbaccatin III 10-O-acetyltransferase. Use of enzyme to couple unnatural acyl-CoA analogues various amino and/or hydroxyl acceptors
synthesis
-
transgenic Flammulina velutipes expressing the DBAT gene is able to produce baccatin III, an advanced precursor of paclitaxel
synthesis
key enzyme in the biosynthesis of the anticancer drug paclitaxel, which catalyses the formation of baccatin III from 10-deacetylbaccatin III
synthesis
mutant enzyme G38R/F301V with a catalytic efficiency approximately six times higher than that of the wild-type is combined with a beta-xylosidase to obtain an in vitro one-pot conversion of 7-beta-xylosyl-10-deacetyltaxol to Taxol yielding 0.64 mg/mlx02taxol in 50 ml at 15 h. This approach represents a promising environmentally friendly alternative for Taxol production from an abundant analogue
synthesis
paclitaxel is a type of broad-spectrum anticancer drug in short supply. The price of acetyl-CoA, which is the acetyl group donor for the enzymatic synthesis of the intermediate, baccatin III, is the bottleneck of the mass production of paclitaxel. The study reports that N-acetyl-D-glucosamineas an acetyl group donor can substantially reduce the cost of production
synthesis
-
transgenic Flammulina velutipes expressing the DBAT gene is able to produce baccatin III, an advanced precursor of paclitaxel
-
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Walker, K.; Croteau, R.
Taxol biosynthetic genes
Phytochemistry
58
1-7
2001
Taxus cuspidata (Q9M6E2)
brenda
Walker.K.; Croteau, R.
Molecular cloning of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus and functional expression in Escherichia coli
Proc. Natl. Acad. Sci. USA
97
583-587
2000
Taxus cuspidata (Q9M6E2), Taxus cuspidata
brenda
Pennington, J.J.; Fett-Neto, A.G.; Nicholson, S.A.; Kingston, D.G.I.; Dicosmo, F.
Acetyl CoA: 10-deacetylbaccatin-III-10-O-acetyltransferase activity in leaves and cell suspension cultures of Taxus cuspidata
Phytochemistry
49
2261-2266
1998
Taxus cuspidata (Q9M6E2)
-
brenda
Fang, J.; Ewald, D.
Expression cloned cDNA for 10-deacetylbaccatin III-10-O-acetyltransferase in Escherichia coli: a comparative study of three fusion systems
Protein Expr. Purif.
35
17-24
2004
Taxus baccata
brenda
DeJong, J.H.; Liu, Y.; Bollon, A.P.; Long, R.M.; Jennewein, S.; Williams, D.; Croteau, R.B.
Genetic engineering of taxol biosynthetic genes in Saccharomyces cerevisiae
Biotechnol. Bioeng.
93
212-224
2006
Taxus brevifolia
brenda
Loncaric, C.; Ward, A.F.; Walker, K.D.
Expression of an acetyl-CoA synthase and a CoA-transferase in Escherichia coli to produce modified taxanes in vivo
Biotechnol. J.
2
266-274
2007
Taxus sp.
brenda
Loncaric, C.; Merriweather, E.; Walker, K.D.
Profiling a taxol pathway 10beta-acetyltransferase: Assessment of the specificity and the production of baccatin III by in vivo acetylation in E. coli
Chem. Biol.
13
309-317
2006
Taxus sp.
brenda
Hao, d.a..C.; Huang, B.; Yang, L.
Phylogenetic relationships of the genus Taxus inferred from chloroplast intergenic spacer and nuclear coding DNA
Biol. Pharm. Bull.
31
260-265
2008
Taxus baccata, Taxus brevifolia, Taxus canadensis, Taxus chinensis, Taxus floridana, Taxus fuana, Taxus globosa, Taxus mairei, Taxus sumatrana, Taxus wallichiana, Taxus wallichiana var. wallichiana
brenda
Hao, D.C.; Huang, B.; Yang, L.
Molecular evolution of paclitaxel biosynthetic genes TS and DBAT of Taxus species
Genetica
135
123-135
2008
Taxus baccata (B5KRG2), Taxus baccata (Q8W169), Taxus brevifolia (B5KRG4), Taxus canadensis (B5KRF5), Taxus chinensis (B5KRF6), Taxus chinensis (B5KRG3), Taxus cuspidata (Q9M6E2), Taxus fuana (B5KRF8), Taxus globosa (B5KRG6), Taxus mairei (B5KRG1), Taxus mairei (Q67DV6), Taxus sumatrana (B5KRG5), Taxus wallichiana var. wallichiana (B5KRF7), Taxus x hunnewelliana (B5KRF3), Taxus x media (A0MPB1)
brenda
Guo, B.; Kai, G.; Gong, Y.; Jin, H.; Wang, Y.; Miao, Z.; Sun, X.; Tang, K.
Molecular cloning and heterologous expression of a 10-deacetylbaccatin III-10-O-acetyl transferase cDNA from Taxus x media
Mol. Biol. Rep.
34
89-95
2007
Taxus x media (Q6SQJ5), Taxus x media
brenda
Zhang, P.; Zhou, P.P.; Yu, L.J.
An endophytic taxol-producing fungus from Taxus media, Cladosporium cladosporioides MD2
Curr. Microbiol.
59
227-232
2009
Cladosporium cladosporioides (B1PBV3), Cladosporium cladosporioides
brenda
Zhang, P.; Zhou, P.P.; Yu, L.J.
An endophytic taxol-producing fungus from Taxus x media, Aspergillus candidus MD3
FEMS Microbiol. Lett.
293
155-159
2009
Aspergillus candidus (B6D960), Aspergillus candidus
brenda
Ondari, M.E.; Walker, K.D.
The taxol pathway 10-O-acetyltransferase shows regioselective promiscuity with the oxetane hydroxyl of 4-deacetyltaxanes
J. Am. Chem. Soc.
130
17187-17194
2008
Taxus cuspidata
brenda
Katkovcinova, Z.; Lazarova, M.; Brunakova, K.; Kosuth, J.; Cellarova, E.
Expression of dbat and dbtnbt genes involved in paclitaxel biosynthesis during the growth cycle of Taxus baccata L. callus cultures
Z. Naturforsch. C
63
721-730
2008
Taxus baccata (Q8W169)
brenda
Zhang, J.; Gong, S.; Guo, Z.
Effects of different elicitors on 10-deacetylbaccatin III-10-O-acetyl transferase activity and cytochrome P450 monooxygenase content in suspension cultures of Taxus cuspidata cells
Cell Biol. Int.
18
e00009
2010
Taxus cuspidata, Taxus cuspidata T5
brenda
Onrubia, M.; Moyano, E.; Bonfill, M.; Palazon, J.; Goossens, A.; Cusido, R.M.
The relationship between TXS, DBAT, BAPT and DBTNBT gene expression and taxane production during the development of Taxus baccata plantlets
Plant Sci.
181
282-287
2011
Taxus baccata
brenda
Han, F.; Kang, L.Z.; Zeng, X.L.; Ye, Z.W.; Guo, L.Q.; Lin, J.F.
Bioproduction of baccatin III, an advanced precursor of paclitaxol, with transgenic Flammulina velutipes expressing the 10-deacetylbaccatin III-10-O-acetyl transferase gene
J. Sci. Food Agric.
94
2376-2383
2014
Taxus chinensis, Taxus chinensis var. mairei
brenda
You, L.; Wei, T.; Zheng, Q.; Lin, J.; Guo, L.; Jiang, B.; Huang, J.
Activity essential residue analysis of taxoid 10beta-O-acetyl transferase for enzymatic synthesis of baccatin
Appl. Biochem. Biotechnol.
186
949-959
2018
Taxus mairei (H9BIH8)
brenda
Sah, B.; Subban, K.; Chelliah, J.
Cloning and sequence analysis of 10-deacetylbaccatin III-10-O-acetyl transferase gene and WRKY1 transcription factor from taxol-producing endophytic fungus Lasiodiplodia theobromea
FEMS Microbiol. Lett.
364
fnx253
2017
Lasiodiplodia theobromae (A0A0D4BT92), Lasiodiplodia theobromae
brenda
Lin, S.L.; Wei, T.; Lin, J.F.; Guo, L.Q.; Wu, G.P.; Wei, J.B.; Huang, J.J.; Ouyang, P.L.
Bio-production of baccatin III, an important precursor of paclitaxel by a cost-effective approach
Mol. Biotechnol.
60
492-505
2018
Taxus mairei (H9BIH8)
brenda
You, L.F.; Wei, T.; Zheng, Q.W.; Jiang, B.H.; Lin, J.F.; Guo, L.Q.
Optimization of baccatin III production by cross-linked enzyme aggregate of taxoid 10beta-O-acetyltransferase
Mol. Biotechnol.
61
p498-505
2019
Taxus mairei (H9BIH8)
brenda
Li, B.J.; Wang, H.; Gong, T.; Chen, J.J.; Chen, T.J.; Yang, J.L.; Zhu, P.
Improving 10-deacetylbaccatin III-10-beta-O-acetyltransferase catalytic fitness for Taxol production
Nat. Commun.
8
15544
2017
Taxus brevifolia (B5KRG4), Taxus canadensis (B5KRF5), Taxus cuspidata (Q9M6E2), Taxus wallichiana (B5KRG1), Taxus x media (Q6SQJ5)
brenda