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Information on EC 2.3.1.140 - rosmarinate synthase
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EC Tree
2.3.1.140 rosmarinate synthaseIUBMB Comments
Involved, with EC 1.1.1.237 (hydroxyphenylpyruvate reductase) in the biosynthesis of rosmarinic acid. Characterized from the plant Melissa officinalis L. (lemon balm).
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Word Map
- 2.3.1.140
-
ammonia-lyase
- officinalis
- lamiaceae
- salvia
- melissa
-
coleus
-
caffeic
- blumei
-
phenylpropanoids
- balm
- 4-coumarate
-
dracocephalum
-
shikimic
- 4-hydroxyphenyllactate
- lemon
-
solenostemon
-
lithospermic
- kotschyi
- synthesis
- miltiorrhiza
-
boiss
-
coumaric
-
smras
- verticillata
-
phenylpropanoid-derived
-
benth
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
rosmarinic acid synthase, hydroxycinnamoyl-coa:hydroxyphenyllactate hydroxycinnamoyltransferase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
4-coumaroyl-CoA:4-hydroxyphenyllactic acid 4-coumaroyl transferase
-
-
-
-
caffeoyl-coenzyme A:3,4-dihydroxyphenyllactic acid caffeoyltransferase
-
-
-
-
hydroxycinnamoyl-CoA:hydroxyphenyllactate hydroxycinnamoyltransferase
Coleus scutellarioides
-
-
RAS
rosmarinic acid synthase
rosmarinic acid synthase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
caffeoyl-CoA + (R)-3-(3,4-dihydroxyphenyl)lactate = CoA + rosmarinate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
caffeoyl-CoA:(R)-3-(3,4-dihydroxyphenyl)lactate 2'-O-caffeoyl-transferase
Involved, with EC 1.1.1.237 (hydroxyphenylpyruvate reductase) in the biosynthesis of rosmarinic acid. Characterized from the plant Melissa officinalis L. (lemon balm).
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CAS REGISTRY NUMBER
COMMENTARY
117590-80-4
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3,4-dihydroxyphenylpropanoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + 2-O-(3,4-dihydroxyphenylpropanoyl)-3-(3,4-dihydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
3,4-dihydroxyphenylpropanoyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-(3,4-dihydroxyphenylpropanoyl)-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
3,4-dihydroxyphenylpropanoyl-CoA + 3-phenyllactate
CoA + 2-O-(3,4-dihydroxyphenylpropanoyl)-3-phenyllactate
Coleus scutellarioides
-
-
-
-
?
4-coumaroyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(3,4-dihydroxyphenyl)lactate
4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate
4-coumaroyl-CoA + D-phenyllactate
CoA + (R)-2-O-(4-coumaroyl)-3-phenyllactate
Coleus scutellarioides
-
-
-
-
?
4-coumaroyl-CoA + D-tyrosine
CoA + N-(4-coumaroyl)-D-tyrosine
Coleus scutellarioides
-
-
-
-
?
4-coumaroyl-CoA + mandelate
CoA + 2-O-(4-coumaroyl)-3-phenyllactate
Coleus scutellarioides
-
-
-
-
?
4-coumaroyl-CoA + phenyllactate
CoA + 2-O-(4-coumaroyl)-3-phenyllactate
Coleus scutellarioides
-
-
-
-
?
4-coumaroyl-CoA + shikimate
CoA + 5-O-(4-coumaroyl)shikimate
-
-
-
?
4-hydroxyphenylpropanoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + 2-O-(4-hydroxyphenylpropanoyl)-3-(3,4-dihydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
4-hydroxyphenylpropanoyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-(4-hydroxyphenylpropanoyl)-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
4-hydroxyphenylpropanoyl-CoA + 3-phenyllactate
CoA + 2-O-(4-hydroxyphenylpropanoyl)-3-phenyllactate
Coleus scutellarioides
-
-
-
-
?
4-hydroxyphenylpropanoyl-CoA + mandelate
CoA + 2-O-(4-hydroxyphenylpropanoyl)mandelate
Coleus scutellarioides
-
-
-
-
?
4-hydroxyphenylpropanoyl-CoA + tyrosol
CoA + 2-O-(4-hydroxyphenylpropanoyl)tyrosol
Coleus scutellarioides
-
-
-
-
?
caffeoyl-CoA + 3-(3-methoxy-4-hydroxyphenyl)lactate
CoA + 2-O-caffeoyl-3-(3-methoxy-4-hydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
caffeoyl-CoA + D-phenyllactate
CoA + (R)-2-O-caffeoyl-3-phenyllactate
Coleus scutellarioides
-
-
-
-
?
cinnamoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + 2-O-cinnamoyl-3-(3,4-dihydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
cinnamoyl-CoA + D-phenyllactate
CoA + (R)-2-O-cinnamoyl-3-phenyllactate
Coleus scutellarioides
-
-
-
-
?
feruloyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + 2-O-feruloyl-3-(3,4-dihydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
feruloyl-CoA + 3-phenyllactate
CoA + 2-O-feruloyl-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
sinapoyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-sinapoyl-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
4-coumaroyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(3,4-dihydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
?
4-coumaroyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(3,4-dihydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
4-coumaroyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(3,4-dihydroxyphenyl)lactate
Coleus scutellarioides
-
81% of the activity with caffeoyl-CoA + 4-hydroxyphenyllactate
-
-
?
4-coumaroyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(3,4-dihydroxyphenyl)lactate
-
-
-
-
?
4-coumaroyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(3,4-dihydroxyphenyl)lactate
-
-
-
-
?
4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
?
4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
79% of the activity with caffeoyl-CoA + 4-hydroxyphenyllactate
-
-
?
4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate
-
-
-
?
4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate
-
-
the precursor for rosmarinic acid synthesis via further hydroxylations, overview
-
?
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + rosmarinate
Coleus scutellarioides
-
-
-
-
?
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + rosmarinate
Coleus scutellarioides
-
-
-
?
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + rosmarinate
Coleus scutellarioides
-
only R(+)-stereoisomer of 3-(3,4-dihydroxyphenyl)lactate is accepted
-
?
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + rosmarinate
Coleus scutellarioides
-
only R(+)-stereoisomer of 3-(3,4-dihydroxyphenyl)lactate is accepted
-
?
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + rosmarinate
Coleus scutellarioides
-
only R(+)-stereoisomer of 3-(3,4-dihydroxyphenyl)lactate is accepted
-
r
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + rosmarinate
Coleus scutellarioides
-
26% of the activity with caffeoyl-CoA + 4-hydroxyphenyllactate
-
-
?
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + rosmarinate
Coleus scutellarioides
-
via intermediate 4-coumaroyl-4'-hydroxyphenyllactic acid
-
-
?
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + rosmarinate
Coleus scutellarioides Benth
-
only R(+)-stereoisomer of 3-(3,4-dihydroxyphenyl)lactate is accepted
-
?
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + rosmarinate
-
-
-
?
caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
?
caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides Benth
-
-
-
-
?
caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate
-
-
-
?
caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate
-
-
-
-
?
caffeoyl-CoA + phenyllactate
CoA + 2-O-caffeoyl-3-phenyllactate
Coleus scutellarioides
-
-
-
-
?
caffeoyl-CoA + phenyllactate
CoA + 2-O-caffeoyl-3-phenyllactate
Coleus scutellarioides
-
-
-
?
cinnamoyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-cinnamoyl-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
cinnamoyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-cinnamoyl-3-(4-hydroxyphenyl)lactate
-
-
-
?
feruloyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-feruloyl-3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
-
-
-
?
feruloyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-feruloyl-3-(4-hydroxyphenyl)lactate
-
-
-
?
additional information
?
-
Coleus scutellarioides
-
probably 4-coumaroyl-CoA and 4-hydroxyphenyllactate are the substrates used in vivo
-
-
?
additional information
?
-
Coleus scutellarioides
-
CbRAS accepts several CoA-activated phenylpropenoic acids as donor substrates and D-(hydroxy)phenyllactates as acceptors resulting in ester formation while shikimate and quinate are not accepted. Amino acids, such as D-phenylalanine, D-tyrosine, and D-DOPA, also yielded products, showing that RAS can putatively catalyze amide formation
-
-
?
additional information
?
-
recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity
-
-
?
additional information
?
-
-
recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity
-
-
?
additional information
?
-
substrate specificity of recombinant AAT1, overview. No or poor activity with anthranilic acid
-
-
?
additional information
?
-
-
substrate specificity of recombinant AAT1, overview. No or poor activity with anthranilic acid
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-coumaroyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(3,4-dihydroxyphenyl)lactate
-
-
-
-
?
4-coumaroyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-(4-coumaroyl)-3-(4-hydroxyphenyl)lactate
-
-
the precursor for rosmarinic acid synthesis via further hydroxylations, overview
-
?
caffeoyl-CoA + 3-(4-hydroxyphenyl)lactate
CoA + 2-O-caffeoyl-3-(4-hydroxyphenyl)lactate
-
-
-
-
?
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + rosmarinate
Coleus scutellarioides
-
via intermediate 4-coumaroyl-4'-hydroxyphenyllactic acid
-
-
?
caffeoyl-CoA + 3-(3,4-dihydroxyphenyl)lactate
CoA + rosmarinate
-
-
-
?
additional information
?
-
Coleus scutellarioides
-
probably 4-coumaroyl-CoA and 4-hydroxyphenyllactate are the substrates used in vivo
-
-
?
additional information
?
-
recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity
-
-
?
additional information
?
-
-
recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4-coumaroyl-CoA
-
severe substrate inhibition of 4-coumaroyl-CoA with 3-(4-hydroxyphenyl)lactate as acceptor and less with 3-(3,4-dihydroxyphenyl)lactate
4-hydroxymercuribenzoate
Coleus scutellarioides
-
complete inhibition at concentrations higher than 0.5 mM
rosmarinic acid
Coleus scutellarioides
-
strong inhibitor, at concentration higher than 0.2 mM, non-competitive
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.035
3-(3,4-Dihydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with 4-coumaroyl-CoA, recombinant RAS
0.0853
3-(3,4-Dihydroxyphenyl)lactate
-
with 4-coumaroyl-CoA, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.1528
3-(3,4-Dihydroxyphenyl)lactate
-
with 4-coumaroyl-CoA, pH 7.0, 30°C, native crude enzyme
0.193
3-(3,4-Dihydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with caffeoyl-CoA, recombinant RAS
0.2126
3-(3,4-Dihydroxyphenyl)lactate
-
with caffeoyl-CoA, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.2336
3-(3,4-Dihydroxyphenyl)lactate
-
with caffeoyl-CoA, pH 7.0, 30°C, native crude enzyme
0.34
3-(3,4-Dihydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with cinnamoyl-CoA, recombinant RAS
1.3
3-(3,4-Dihydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with feroyl-CoA, recombinant RAS
0.0169
3-(4-hydroxyphenyl)lactate
-
with 4-coumaroyl-CoA, pH 7.0, 30°C, native crude enzyme
0.0189
3-(4-hydroxyphenyl)lactate
-
with 4-coumaroyl-CoA, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.021
3-(4-hydroxyphenyl)lactate
with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1
0.025
3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with 4-coumaroyl-CoA, recombinant RAS
0.049
3-(4-hydroxyphenyl)lactate
with caffeoyl-CoA, pH 7.5, 30°C, recombinant AAT1
0.074
3-(4-hydroxyphenyl)lactate
-
with caffeoyl-CoA, pH 7.0, 30°C, native crude enzyme
0.1565
3-(4-hydroxyphenyl)lactate
-
with caffeoyl-CoA, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.163
3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with caffeoyl-CoA, recombinant RAS
0.55
3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with cinnamoyl-CoA, recombinant RAS
0.652
3-(4-hydroxyphenyl)lactate
with cinnamoyl-CoA, pH 7.5, 30°C, recombinant AAT1
0.672
3-(4-hydroxyphenyl)lactate
with feruloyl-CoA, pH 7.5, 30°C, recombinant AAT1
1.425
3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with feroyl-CoA, recombinant RAS
0.0013
4-coumaroyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(3,4-dihydroxyphenyl)lactate, native RAS
0.0018
4-coumaroyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(3,4-dihydroxyphenyl)lactate, recombinant RAS
0.0032
4-coumaroyl-CoA
-
with 3-(3,4-dihydroxyphenyl)lactate, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.0047
4-coumaroyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(4-hydroxyphenyl)lactate, native RAS
0.0048
4-coumaroyl-CoA
-
with 3-(4-hydroxyphenyl)lactate, pH 7.0, 30°C, native crude enzyme
0.0073
4-coumaroyl-CoA
-
with 3-(3,4-dihydroxyphenyl)lactate, pH 7.0, 30°C, native crude enzyme
0.0092
4-coumaroyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(4-hydroxyphenyl)lactate, recombinant RAS
0.0094
4-coumaroyl-CoA
-
with 3-(4-hydroxyphenyl)lactate, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.0107
4-coumaroyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with D-phenyllactate, recombinant RAS
0.014
4-coumaroyl-CoA
with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1
0.0817
4-coumaroyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with D-tyrosine, recombinant RAS
0.083
4-coumaroyl-CoA
with shikimate, pH 7.5, 30°C, recombinant AAT1
0.0021
caffeoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(4-hydroxyphenyl)lactate, recombinant RAS
0.0023
caffeoyl-CoA
-
with 3-(4-hydroxyphenyl)lactate, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.0033
caffeoyl-CoA
-
with 3-(3,4-dihydroxyphenyl)lactate, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.0034
caffeoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(4-hydroxyphenyl)lactate, native RAS
0.0035
caffeoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with D-phenyllactate, recombinant RAS
0.0039
caffeoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(3,4-dihydroxyphenyl)lactate, recombinant RAS
0.004
caffeoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(3,4-dihydroxyphenyl)lactate, native RAS
0.0052
caffeoyl-CoA
-
with 3-(3,4-dihydroxyphenyl)lactate, pH 7.0, 30°C, native crude enzyme
0.0058
caffeoyl-CoA
-
with 3-(4-hydroxyphenyl)lactate, pH 7.0, 30°C, native crude enzyme
0.05
caffeoyl-CoA
with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1
0.0158
cinnamoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(3,4-dihydroxyphenyl)lactate, recombinant RAS
0.025
cinnamoyl-CoA
with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1
0.0255
cinnamoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(4-hydroxyphenyl)lactate, recombinant RAS
0.0529
cinnamoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with D-phenyllactate, recombinant RAS
0.49
D-phenyllactate
Coleus scutellarioides
-
pH 7.0, 30°C, with 4-coumaroyl-CoA, recombinant RAS
1.346
D-phenyllactate
Coleus scutellarioides
-
pH 7.0, 30°C, with cinnamoyl-CoA, recombinant RAS
17.82
D-phenyllactate
Coleus scutellarioides
-
pH 7.0, 30°C, with caffeoyl-CoA, recombinant RAS
0.0191
feruloyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(3,4-dihydroxyphenyl)lactate, recombinant RAS
0.022
feruloyl-CoA
with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1
0.0364
feruloyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(4-hydroxyphenyl)lactate, recombinant RAS
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Coleus scutellarioides
Coleus scutellarioides
enzyme is induced by 4% sucrose content of the medium
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
RAS activity decreases through the cell cultivation period, it is highest after 3-4 days and completely declined after 11 days, overview. The RAS expression level declines also durring cell cultivation and is almost undetectable after 6-9 days, and absent after 10 days
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
metabolism
-
the enzyme catalyzes a step in the rosmarinic acid biosynthesis, pathway overview
evolution
-
MoRAS belongs to the BAHD superfamily of acyltransferases, it possesses the two conserved sequence motifs HxxxDG and DFGWG
evolution
Coleus scutellarioides
-
RAS belongs to the large plant acyl-CoA-dependent BAHD acyltransferase superfamily characterized by mostly conserved sequence motifs, the HxxxD(G) motif containing the catalytically active histidine residue and the DFGWG motif close to the C terminus, phylogenetic analysis, overview
evolution
rosmarinic acid synthase is a member of the plant BAHD family, it contains the BAHD family HXXXD motif (amino acids 180-184), with the catalytically active histidine and the DFGWG motif that is thought to be responsible for the steric position of the active site, but it additionally contains 25 additional amino acids in the section following the HXXXD motif, phylogenetic tree of BAHD acyltransferases, overview
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). RAS_MELOI
427
0
47161
Swiss-Prot
other Location (Reliability: 2)
RAS_PLESU
430
0
47902
Swiss-Prot
other Location (Reliability: 2)
A0A650AGZ5_9LAMI
425
0
47569
TrEMBL
other Location (Reliability: 2)
A0A0A7RA06_SALMI
427
0
47371
TrEMBL
other Location (Reliability: 3)
A0A396HKC3_MEDTR
360
0
41127
TrEMBL
other Location (Reliability: 4)
F2YNI5_LAVAN
460
0
50678
TrEMBL
other Location (Reliability: 3)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5 - 10
Coleus scutellarioides
-
-
486321
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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