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Information on EC 2.3.1.115 - isoflavone-7-O-beta-glucoside 6''-O-malonyltransferase
for references in articles please use BRENDA:EC2.3.1.115
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EC Tree 2 Transferases
2.3 Acyltransferases
2.3.1 Transferring groups other than aminoacyl groups
2.3.1.115 isoflavone-7-O-beta-glucoside 6''-O-malonyltransferase
IUBMB Comments
The 6-position of the glucose residue of formononetin can also act as acceptor; some other 7-O-glucosides of isoflavones, flavones and flavonols can also act, but more slowly.
The enzyme appears in viruses and cellular organisms
- 2.3.1.115
-
chanarin-dorfman
-
droplets
- triglyceride
-
lipolysis
- triacylglycerols
-
ichthyosis
-
domain-containing
-
ichthyosiform
-
erythroderma
-
cirrhosis
-
steatosis
-
multisystemic
-
co-activator
-
lysophosphatidic
-
non-bullous
-
phosphatidic
- nutrition
-
hepatomegaly
Reaction Schemes
showSynonyms
mat-7, mtmat1, malonyl-coa:flavone/flavonol 7-o-glucoside malonyltransferase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
flavone/flavonol 7-O-beta-D-glucoside malonyltransferase
-
-
-
-
malonyl CoA:isoflavone 7-O-glucoside-6''-O-malonyltransferase
-
malonyl-CoA:flavone/flavonol 7-O-glucoside malonyltransferase
-
-
-
-
malonyl-coenzyme A:flavone/flavonol-7-O-glycoside malonyltransferase
-
-
-
-
malonyl-coenzyme A:isoflavone 7-O-glucoside-6''-malonyltransferase
-
-
-
-
malonyltransferase, flavone (flavonol) 7-O-glycoside
-
-
-
-
malonyltransferase, isoflavone 7-O-glucoside 6''-O-
-
-
-
-
MAT-7
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
malonyl-CoA + biochanin A 7-O-beta-D-glucoside = CoA + biochanin A 7-O-(6-O-malonyl-beta-D-glucoside)
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
MetaCyc
apigenin glycosides biosynthesis, biochanin A conjugates interconversion, daidzein conjugates interconversion, formononetin conjugates interconversion, genistein conjugates interconversion, maackiain conjugates interconversion, medicarpin conjugates interconversion
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SYSTEMATIC NAME
IUBMB Comments
malonyl-CoA:isoflavone-7-O-beta-D-glucoside 6''-O-malonyltransferase
The 6-position of the glucose residue of formononetin can also act as acceptor; some other 7-O-glucosides of isoflavones, flavones and flavonols can also act, but more slowly.
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CAS REGISTRY NUMBER
COMMENTARY
78413-09-9
-
93585-97-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
apigenin 7-O-apiosylglucoside + malonyl-CoA
apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
apiin + malonyl-CoA
apigenin 7-O-apiosylglucoside -6''-O-malonylester + malonyl-CoA
-
Substrates: i.e. apigenin 7-O-apiosylglucoside, best substrate
Products: -
Products: -
?
diosmetin 7-O-glucoside + malonyl-CoA
CoA + diosmetin 7-O-glucoside 6''-O-malonylester
-
Substrates: 72% of the activity with apiin
Products: -
Products: -
?
isorhamnetin 3-O-glucoside + malonyl-CoA
CoA + isorhamnetin 3-O-glucoside 6''-O-malonylester
-
Substrates: 11% of the activity with apiin
Products: -
Products: -
?
isorhamnetin 7-O-glucoside + malonyl-CoA
CoA + isorhamnetin 7-O-glucoside 6''-O-malonylester
-
Substrates: 12% of the activity with apiin
Products: -
Products: -
?
kaempferol 3-O-glucoside + malonyl-CoA
CoA + kaempferol 3-O-glucoside 6''-O-malonylester
-
Substrates: 15% of the activity with apiin
Products: -
Products: -
?
malonyl CoA + genistein 7-O-glucoside
CoA + genistein 7-O-glucoside 6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
malonyl-CoA + (-)-maackiain 3-O-beta-D-glucoside
CoA + (-)-maackiain 3-O-(6-O-malonyl)-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
malonyl-CoA + 2',4,4'-trihydroxychalcone 4-O-glucoside
CoA + 2',4,4'-trihydroxychalcone 4-O-glucoside 6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
malonyl-CoA + 6,4'-dihydroxy-7-O-glucosyl isoflavone
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
malonyl-CoA + biochanin A 7-O-glucoside
CoA + biochanin A 7-O-glucoside-6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
malonyl-CoA + biochanin A-beta-D-glucoside
coenzyme A + biochanin A 7-O-(6-O-malonyl-beta-D-glucoside)
-
Substrates: -
Products: -
Products: -
?
malonyl-CoA + cyanidin 3,5-O-di-beta-D-glucoside
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
malonyl-CoA + daidzin
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
malonyl-CoA + formononetin 7-O-glucoside
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
malonyl-CoA + formononetin 7-O-glucoside
CoA + formononetin 7-O-glucoside-6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
malonyl-CoA + genistein 7-O-glucoside
CoA + genistein 7-O-glucoside 6''-O-malonylester
Substrates: -
Products: -
Products: -
?
malonyl-CoA + genistin
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
malonyl-CoA + glycitein 7-O-glucoside
CoA + glycitein 7-O-glucoside 6''-O-malonylester
Substrates: -
Products: -
Products: -
?
malonyl-CoA + hyperoside
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
malonyl-CoA + isoflavone
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
malonyl-CoA + isoquercitrin
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
malonyl-CoA + kaempferol 7-O-glucoside
CoA + kaempferol 7-O-glucoside 6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
malonyl-CoA + keracyanin
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
malonyl-CoA + kuromanin
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
malonyl-CoA + naringenin 7-O-glucoside
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
malonyl-CoA + naringenin 7-O-neohesperidoside
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
malonyl-CoA + orobol 7-O-glucoside
CoA + orobol 7-O-glucoside 6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
malonyl-CoA + pratensein 7-O-glucoside
CoA + pratensein 7-O-glucoside 6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
malonyl-CoA + rutin
?
Substrates: assay at pH 8.0, 30°C
Products: -
Products: -
?
naringenin 7-O-glucoside + malonyl-CoA
CoA + naringenin 7-O-glucoside 6''-O-malonylester
-
Substrates: 16% of the activity with apiin
Products: -
Products: -
?
quercetagetin 7-O-glucoside + malonyl-CoA
CoA + quercetagetin 7-O-glucoside 6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
quercetin 3-O-glucoside + malonyl-CoA
CoA + quercetin 3-O-glucoside 6''-O-malonylester
-
Substrates: 8% of the activity with apiin
Products: -
Products: -
?
quercetin 7-O-glucoside + malonyl-CoA
CoA + quercetin 7-O-glucoside 6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
succinyl-CoA + biochanin A-beta-D-glucoside
coenzyme A + biochanin A 7-O-(6-O-succinyl-beta-D-glucoside)
-
Substrates: -
Products: -
Products: -
?
apigenin 7-O-apiosylglucoside + malonyl-CoA
apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
Substrates: i.e. apiin
Products: -
Products: -
?
apigenin 7-O-apiosylglucoside + malonyl-CoA
apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
Substrates: -
Products: -
Products: -
?
apigenin 7-O-glucoside + malonyl-CoA
CoA + apigenin 7-O-glucoside 6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
apigenin 7-O-glucoside + malonyl-CoA
CoA + apigenin 7-O-glucoside 6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
apigenin 7-O-glucoside + malonyl-CoA
CoA + apigenin 7-O-glucoside 6''-O-malonylester
-
Substrates: 88% of the activity with apiin
Products: -
Products: -
?
luteolin 7-O-glucoside + malonyl-CoA
CoA + luteolin 7-O-glucoside 6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
luteolin 7-O-glucoside + malonyl-CoA
CoA + luteolin 7-O-glucoside 6''-O-malonylester
-
Substrates: 30% of the activity with apiin
Products: -
Products: -
?
malonyl-CoA + daidzein 7-O-glucoside
CoA + daidzein 7-O-glucoside 6''-O-malonylester
-
Substrates: -
Products: -
Products: -
?
malonyl-CoA + daidzein 7-O-glucoside
CoA + daidzein 7-O-glucoside 6''-O-malonylester
Substrates: -
Products: -
Products: -
?
additional information
?
-
-
Substrates: isoflavones with the glucosyl substituent in position 4' don't act as acceptors
Products: -
Products: -
?
additional information
?
-
-
Substrates: succinyl-CoA can replace malonyl-CoA with lower activity
Products: -
Products: -
?
additional information
?
-
Substrates: IMaT1 prefers to use malonyl-CoA, rather than acetyl CoA, as an acyl donor to convert only isoflavone 7-O-glucosides, but not other flavonoid glucosides
Products: -
Products: -
?
additional information
?
-
Substrates: IMaT1 prefers to use malonyl-CoA, rather than acetyl CoA, as an acyl donor to convert only isoflavone 7-O-glucosides, but not other flavonoid glucosides
Products: -
Products: -
?
additional information
?
-
-
Substrates: 4-hydroxy-2,5-dichlorophenoxyacetic acid glucoside and 4-methylumbelliferyl glucoside are not accepted as substrate
Products: -
Products: -
?
additional information
?
-
-
Substrates: last step in biosynthesis of flavonoid glycosides
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
apigenin 7-O-apiosylglucoside + malonyl-CoA
apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
additional information
?
-
-
Substrates: last step in biosynthesis of flavonoid glycosides
Products: -
Products: -
?
apigenin 7-O-apiosylglucoside + malonyl-CoA
apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
Substrates: i.e. apiin
Products: -
Products: -
?
apigenin 7-O-apiosylglucoside + malonyl-CoA
apigenin 7-O-apiosylglucoside -6''-O-malonylester + CoA
-
Substrates: -
Products: -
Products: -
?
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
the IF7MaT activity increases by 27.4 % after UV B treatment and 22.1 % after H2 treatment. Effects of combined UV-B + H2 treatment do not differ significantly
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0183
malonyl-CoA
cosubstrate genistein 7-O-glucoside, pH 7.2, 30°C
0.0256
malonyl-CoA
cosubstrate genistein 7-O-glucoside, pH 7.2, 30°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
8.1
malonyl-CoA
cosubstrate genistein 7-O-glucoside, pH 7.2, 30°C
19.21
malonyl-CoA
cosubstrate genistein 7-O-glucoside, pH 7.2, 30°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
440
malonyl-CoA
cosubstrate genistein 7-O-glucoside, pH 7.2, 30°C
750
malonyl-CoA
cosubstrate genistein 7-O-glucoside, pH 7.2, 30°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
transgenic hairy roots overexpressing IMaT1 have increased levels of malonyldaidzin and malonylgenistin. Knockdown of IMaT1 reduces malonyldaidzin and malonylgenistin contents, and affects other isoflavonoids differently
physiological function
transgenic hairy roots overexpressing IMaT3 have increased levels of malonyldaidzin and malonylgenistin. Knockdown of IMaT3 reduces malonyldaidzin and malonylgenistin contents, and affects other isoflavonoids differently
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A7BIC9_SOYBN
467
0
51664
TrEMBL
other Location (Reliability: 2)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50000
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
most stable in Tris buffer at pH 8, unstable to repeated freezing and thawing, dithioerythritol stabilizes
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, partially purified enzyme is stable for several months, extensively purified enzyme loses 50% of activity within 2-3 days
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
high expression in root and leaf, induced expression by copper treatment, slightly induced expression after elicitation of a root cell suspension culture by yeast elicitor
IF7MaT gene decreases by 69.1 % after H2 treatment and 66 % after UV B + H2 treatment compared with the control on day 4 of germination.UV-B treatment alone has no significant effect
induced expression by drought stress and fungal infection
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Matern, U.; Feser, C.; Hammer, D.
Further characterization and regulation of malonyl-coenzyme A: flavonoid glucoside malonyltransferases from parsley cell suspension cultures
Arch. Biochem. Biophys.
226
206-217
1983
Petroselinum crispum
brenda
Matern, U.; Potts, J.R.M.; Hahlbrock, K.
Two flavonoid-specific malonyltransferases from cell suspension cultures of Petroselinum hortense: partial purification and some properties of malonyl-coenzyme A: flavone/flavonol-7-O-glycoside malonyltransferase and malonyl-coenzyme A: flavonol-3-O-glucoside malonyltransferase
Arch. Biochem. Biophys.
208
233-241
1981
Petroselinum crispum
brenda
Koester, J.; Bussmann, R.; Barz, W.
Malonyl-coenzyme A:isoflavone 7-O-glucoside-6-O-malonyltransferase from roots of chick pea (Cicer arietinum L.)
Arch. Biochem. Biophys.
234
513-521
1984
Cicer arietinum
brenda
Yu, X.; Chen, M.; Liu, C.
Nucleocytoplasmic-localized acyltransferases catalyze the malonylation of 7-O-glycosidic (iso)flavones in Medicago truncatula
Plant J.
55
382-396
2008
Medicago truncatula (B4Y0U0), Medicago truncatula (B4Y0U2), Medicago truncatula (B4Y0U1), Medicago truncatula
brenda
Ahmad, M.Z.; Li, P.; Wang, J.; Rehman, N.U.; Zhao, J.
Isoflavone malonyltransferases GmIMaT1 and GmIMaT3 differently modify isoflavone glucosides in soybean (Glycine max) under various stresses
Front. Plant Sci.
8
735
2017
Glycine max (A0A1X9JQM7), Glycine max (A0A1Y9TFD9)
brenda
Xu, W.; Li, M.; Li, W.; Liu, H.; Xu, X.; Yang, T.; Ma, M.
Effect of H2 treatment under UV-B irradiation on the enrichment of germinated soybean isoflavones and mechanisms based on growth state, antioxidant system, and metabolomics
LEBENSM.WISS.TECHNOL.
195
115821
2024
Glycine max (A7BIC9)
-
brenda
EXTERNAL LINKS (specific for EC-Number 2.3.1.115)
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