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Information on EC 2.3.1.108 - alpha-tubulin N-acetyltransferase
for references in articles please use BRENDA:EC2.3.1.108
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EC Tree 2 Transferases
2.3 Acyltransferases
2.3.1 Transferring groups other than aminoacyl groups
2.3.1.108 alpha-tubulin N-acetyltransferase
IUBMB Comments
The enzyme is conserved from protists to mammals and is present in flowering plants. In most organisms it acetylates L-lysine at position 40 of α-tubulin.
The enzyme appears in viruses and cellular organisms
- 2.3.1.108
- trna
- histone
- chromatin
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anticodons
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wobble
- trnamet
- microtubule
- dysautonomia
-
aminoacylated
-
formylation
- cbp
- aminoacyl-trnas
- kluyveromyces
-
zymocin
- methionyl-trna
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ikbkap
- trnaphe
- nua4
- rnapii
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six-subunit
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p-site
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non-histone
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complex-associated
Reaction Schemes
showSynonyms
elongator, lysine acetyltransferase, atat1, mec-17, tubulin acetyltransferase, alpha-tubulin acetyltransferase, atat-2, alpha-tubulin n-acetyltransferase 1, alpha-tubulin n-acetyltransferase, alpha-tubulin acetylase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetyl-CoA:alpha-tubulin-L-lysine Ne-acetyltransferase
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alpha-TAT
alpha-tubulin acetylase
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-
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alpha-tubulin acetyltransferase
alpha-tubulin N-acetyltransferase 1
alphaTAT
alphaTAT1
ATAT1
Mec-17
alpha-tubulin acetyltransferase
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + [alpha-tubulin]-L-lysine = CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:[alpha-tubulin]-L-lysine N6-acetyltransferase
The enzyme is conserved from protists to mammals and is present in flowering plants. In most organisms it acetylates L-lysine at position 40 of alpha-tubulin.
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CAS REGISTRY NUMBER
COMMENTARY
99889-90-4
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + [alpha-TAT1]-L-lysine
CoA + [alpha-TAT1]-N6-acetyl-L-lysine
Substrates: -
Products: enzyme TAT1 acetylates itself in a regulatory mechanism that is required for effective modification of tubulin. Acetylation of multiple lysine residues on itself
Products: enzyme TAT1 acetylates itself in a regulatory mechanism that is required for effective modification of tubulin. Acetylation of multiple lysine residues on itself
?
acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
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Substrates: -
Products: -
Products: -
?
acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
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Substrates: acetylation of the lysine residue at position 40
Products: -
Products: -
?
acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
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Substrates: alpha-tubulin from calf is acetylated
Products: -
Products: -
?
acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
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Substrates: alpha-tubulin from mouse is acetylated
Products: -
Products: -
?
acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
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Substrates: alpha-tubulin from calf is acetylated
Products: -
Products: -
?
acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
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Substrates: alpha-tubulin from Chlamydomonas is acetylated
Products: -
Products: -
?
acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
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Substrates: post-translational modification of alpha-tubulin
Products: -
Products: -
?
acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
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Substrates: alpha-tubulin from calf is acetylated
Products: -
Products: -
?
acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
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Substrates: alpha-tubulin from Chlamydomonas is acetylated
Products: -
Products: -
?
acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
Substrates: -
Products: -
Products: -
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acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
Substrates: -
Products: alpha-tubulin residue Ser38 is crucial for substrate recognition,whereas Asp39, Ile42, the glycine stretch (amino acid residues 4345) and Asp46 are also involved
Products: alpha-tubulin residue Ser38 is crucial for substrate recognition,whereas Asp39, Ile42, the glycine stretch (amino acid residues 4345) and Asp46 are also involved
?
acetyl-CoA + cortactin
CoA + N-acetyl-cortactin
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Substrates: ATAT1 acetylates, binds and colocalizes with cortactin at the adherent surface of MDA-MB-231 cells
Products: -
Products: -
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acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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Substrates: -
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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Substrates: acetylation of the epsilon-amino group of Lys40
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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Substrates: K40 of alpha-tubulin is the sole site of acetylation by alphaTAT1, alphaTAT1 specifically acetylates K40 of alpha-tubulin and prefers microtubules over free tubulin, overview
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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Substrates: alphaTAT1 acetylates tubulin through its GNAT domain in vitro. K40 of alpha-tubulin is the sole site of acetylation by alphaTAT1. alphaTAT1 displays a greater catalytic efficiency for taxol-stabilized microtubules than for free tubulin
Products: -
Products: -
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acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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Substrates: acetylation of the epsilon-amino group of Lys40
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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Substrates: acetylation of the epsilon-amino group of Lys40
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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Substrates: -
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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Substrates: acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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Substrates: acetylation of the epsilon-amino group of Lys40
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
Substrates: acetylation of the epsilon-amino group of Lys40
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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Substrates: acetylation of the epsilon-amino group of Lys40, in Tetrahymena, alpha-tubulin is the major if not the only substrate of MEC-17-dependent K acetylation
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine40
CoA + [alpha-tubulin]-N6-acetyl-L-lysine40
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Substrates: -
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine40
CoA + [alpha-tubulin]-N6-acetyl-L-lysine40
Substrates: -
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine40
CoA + [alpha-tubulin]-N6-acetyl-L-lysine40
Substrates: -
Products: -
Products: -
?
additional information
?
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Substrates: alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
Products: -
Products: -
?
additional information
?
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Substrates: wild-type adults have a strong signal for acetylated alpha-tubulin in the six touch receptor neurons
Products: -
Products: -
?
additional information
?
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Substrates: in vitro, MEC-17 exclusively acetylates Lys40 of alpha-tubulin
Products: -
Products: -
?
additional information
?
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Substrates: alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
Products: -
Products: -
?
additional information
?
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Substrates: the enzyme shows tubulin-specific acetylation activity
Products: -
Products: -
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additional information
?
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Substrates: the enzyme shows tubulin-specific acetylation activity
Products: -
Products: -
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additional information
?
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Substrates: recombinant GST-MEC-17 directly acetylates purified tubulin from the MEC17-KO strain in vitro
Products: -
Products: -
?
additional information
?
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Substrates: alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + cortactin
CoA + N-acetyl-cortactin
-
Substrates: ATAT1 acetylates, binds and colocalizes with cortactin at the adherent surface of MDA-MB-231 cells
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine40
CoA + [alpha-tubulin]-N6-acetyl-L-lysine40
Substrates: -
Products: -
Products: -
?
acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
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Substrates: -
Products: -
Products: -
?
acetyl-CoA + alpha-tubulin L-lysine
CoA + alpha-tubulin N6-acetyl-L-lysine
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Substrates: post-translational modification of alpha-tubulin
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
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Substrates: acetylation of the epsilon-amino group of Lys40
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
Substrates: K40 of alpha-tubulin is the sole site of acetylation by alphaTAT1, alphaTAT1 specifically acetylates K40 of alpha-tubulin and prefers microtubules over free tubulin, overview
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
Substrates: acetylation of the epsilon-amino group of Lys40
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
Substrates: acetylation of the epsilon-amino group of Lys40
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
Substrates: acetylation of alpha-tubulin mostly occurs on lysine residue 40, which is localized in the microtubule lumen
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
Substrates: acetylation of the epsilon-amino group of Lys40
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
Substrates: -
Products: -
Products: -
?
acetyl-CoA + [alpha-tubulin]-L-lysine
CoA + [alpha-tubulin]-N6-acetyl-L-lysine
-
Substrates: acetylation of the epsilon-amino group of Lys40, in Tetrahymena, alpha-tubulin is the major if not the only substrate of MEC-17-dependent K acetylation
Products: -
Products: -
?
additional information
?
-
-
Substrates: alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
Products: -
Products: -
?
additional information
?
-
-
Substrates: wild-type adults have a strong signal for acetylated alpha-tubulin in the six touch receptor neurons
Products: -
Products: -
?
additional information
?
-
-
Substrates: alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
Products: -
Products: -
?
additional information
?
-
-
Substrates: alphaTAT/MEC-17 is a lysine acetyltransferase for tubulin and not histones
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ca2+
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at 1 mM, almost complete inhibition; inhibition is due to the binding of calcium to the tubulin dimer rather than to the enzyme itself
additional information
analysis of KATi, bisubstrate analogues, natural compounds and synthetic derivatives, mechanism of action, structure-activity relationships, and pharmacokinetic/pharmacodynamic properties, overview
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additional information
-
analysis of KATi, bisubstrate analogues, natural compounds and synthetic derivatives, mechanism of action, structure-activity relationships, and pharmacokinetic/pharmacodynamic properties, overview
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
lipopolysaccharide
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macrophages challenged by bacterial lipopolysaccharides undergo extensive microtubule acetylation
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE