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IUBMB CommentsThe plant enzyme, characterized from Brassicaceae, is involved in secondary metabolism.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
1-(hydroxycinnamoyl)-glucose:1-(hydroxycinnamoyl)-glucose hydroxycinnamoyltransferase, 1-O-(4-hydroxy-3,5-dimethoxycinnamoyl)-beta-D-glucoside:1-O-(4-hydroxy-3,5-dimethoxycinnamoyl)-beta-D-glucoside 1-O-sinapoyltransferase, CGT, hydroxycinnamoyltransferase, hydroxycinnamoylglucose-hydroxycinnamoylglucose, more
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1-(hydroxycinnamoyl)-glucose:1-(hydroxycinnamoyl)-glucose hydroxycinnamoyltransferase
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1-O-(4-hydroxy-3,5-dimethoxycinnamoyl)-beta-D-glucoside:1-O-(4-hydroxy-3,5-dimethoxycinnamoyl)-beta-D-glucoside 1-O-sinapoyltransferase
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hydroxycinnamoyltransferase, hydroxycinnamoylglucose-hydroxycinnamoylglucose
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2 1-O-sinapoyl-beta-D-glucose = D-glucose + 1,2-bis-O-sinapoyl-beta-D-glucose
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Acyl group transfer
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sinapoyl group transfer
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MetaCyc
sinapate ester biosynthesis
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1-O-(4-hydroxy-3,5-dimethoxycinnamoyl)-beta-D-glucoside:1-O-(4-hydroxy-3,5-dimethoxycinnamoyl-beta-D-glucoside 1-O-sinapoyltransferase
The plant enzyme, characterized from Brassicaceae, is involved in secondary metabolism.
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1-(p-coumaroyl)-beta-D-glucose + 1-(p-coumaroyl)-beta-D-glucose
1,2-di-O-p-coumaroyl-beta-D-glucopyranose + D-glucose
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Substrates: -
Products: -
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1-feruloyl-beta-D-glucose + 1-feruloyl-beta-D-glucose
1,2-di-O-feruloyl-beta-glucopyranose + glucose
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Substrates: -
Products: -
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1-O-sinapoyl-beta-D-glucopyranose + 1-O-sinapoyl-beta-D-glucopyranose
1,2-di-O-sinapoyl-beta-D-glucopyranose + glucose
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Substrates: -
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additional information
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Substrates: strict specificity of acyl transfer to the C2 hydroxyl group of the acceptor glucose molecule
Products: -
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1-O-sinapoyl-beta-D-glucopyranose + 1-O-sinapoyl-beta-D-glucopyranose
1,2-di-O-sinapoyl-beta-D-glucopyranose + glucose
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Substrates: -
Products: -
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2-mercaptoethanol
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1 mM, total inhibition
Ca2+
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divalent cations at concentrations of more than 5 mM are inhibitory, 10 mM, 45% remaining activity
Co2+
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divalent cations at concentrations of more than 5 mM are inhibitory, 10 mM, total inhibition
dithiothreitol
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1 mM, total inhibition
Mg2+
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divalent cations at concentrations of more than 5 mM are inhibitory, 10 mM, 65% remaining activity
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additional information
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no requirement for divalent cations or thiols
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0.58
1-(p-coumaroyl)-glucose
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0.4
1-feruloyl-beta-D-glucose
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0.42
1-sinapoyl-beta-D-glucose
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7 - 9
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50% of maximal activity at pH 7.0 and pH 9.0
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L. var. sativus cv. Saxa
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brenda
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dark grown seedlings show higher activity than light grown seedlings
brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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SCP8_ARATH
433
0
49439
Swiss-Prot
Secretory Pathway (Reliability: 1)
SCP9_ARATH
437
0
49756
Swiss-Prot
Secretory Pathway (Reliability: 2)
SCP13_ARATH
430
0
49150
Swiss-Prot
Secretory Pathway (Reliability: 1)
C0G721_9HYPH
581
0
62460
TrEMBL
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C4IRY4_BRUAO
581
0
62448
TrEMBL
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C4WKT1_9HYPH
572
0
62020
TrEMBL
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A0A2G9HLH2_9LAMI
201
0
23723
TrEMBL
other Location (Reliability: 4)
A0A396J2S1_MEDTR
189
0
20966
TrEMBL
Secretory Pathway (Reliability: 1)
A0A2G9G7X6_9LAMI
114
0
13185
TrEMBL
other Location (Reliability: 3)
C0RJQ2_BRUMB
Brucella melitensis biotype 2 (strain ATCC 23457)
581
0
62462
TrEMBL
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A0A2G9HLH0_9LAMI
135
1
14798
TrEMBL
Secretory Pathway (Reliability: 3)
A0A2G9HZX6_9LAMI
243
0
27159
TrEMBL
Secretory Pathway (Reliability: 1)
A0A2P6S143_ROSCH
124
1
14410
TrEMBL
Secretory Pathway (Reliability: 3)
A0A7C9EKH6_OPUST
108
0
11657
TrEMBL
other Location (Reliability: 3)
A0A2G9I9S1_9LAMI
389
0
44444
TrEMBL
Secretory Pathway (Reliability: 1)
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55000
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gel filtration, SDS-PAGE
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monomer
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1 * 55000, SDS-PAGE
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-20°C, presence of 3 mg/ml bovine serum albumin, at least 1 year
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Dahlbender, B.; Strack, D.
Purification and properties of 1-(hydroxycinnamoyl)-glucose:1-(hydroxycinnamoyl)-glucose hydroxycinnamoyl-transferase from radish seedlings
Phytochemistry
25
1043-1046
1986
Raphanus sativus
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brenda
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