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Information on EC 2.1.3.7 - 3-hydroxymethylcephem carbamoyltransferase
for references in articles please use BRENDA:EC2.1.3.7
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EC Tree 2 Transferases
2.1 Transferring one-carbon groups
2.1.3 Carboxy- and carbamoyltransferases
2.1.3.7 3-hydroxymethylcephem carbamoyltransferase
IUBMB Comments
Acts on a wide range of 3-hydroxymethylcephems (a subclass of the cephalosporin antibiotics). Activated by ATP.
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Reaction Schemes
showSynonyms
carbamoyltransferase, 3-hyroxymethylcephem, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
carbamoyltransferase, 3-hyroxymethylcephem
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
carbamoyl phosphate + a 3-hydroxymethylceph-3-em-4-carboxylate = phosphate + a 3-carbamoyloxymethylcephem
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
carbamoyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
cephamycin C biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
carbamoyl-phosphate:3-hydroxymethylceph-3-em-4-carboxylate carbamoyltransferase
Acts on a wide range of 3-hydroxymethylcephems (a subclass of the cephalosporin antibiotics). Activated by ATP.
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CAS REGISTRY NUMBER
COMMENTARY
74315-96-1
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
carbamoyl phosphate + 7-aminodeacetylcephalosporanic acid
7-beta-acylamino-3-carbamoyloxymethylcephem + phosphate
-
Substrates: -
Products: -
Products: -
?
carbamoyl phosphate + 7beta-acylamino-3-hydroxymethylcephem
7beta-acylamino-3-carbamoyloxymethylcephem + phosphate
-
Substrates: -
Products: -
Products: -
?
carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate
phosphate + a 3-carbamoyloxymethylcephem
carbamoyl phosphate + decarbamoylcefuroxime
carbamoylcefuroxime + phosphate
-
Substrates: -
Products: -
Products: -
?
carbamoyl phosphate + [3-hydroxymethyl]deacetylcephalosporin C
7beta-(5-D-aminoadipamido)-3-carbamoyloxymethylceph-3-em-4-carboxylic acid + phosphate
-
Substrates: ATP may be a substrate or an effector
Products: -
Products: -
?
carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate
phosphate + a 3-carbamoyloxymethylcephem
-
Substrates: the cmcH gene - encoding 3-hydroxymethylcephem carbamoyltransferase - upstream region does not contain a functional promoter, suggesting that cmcH is transcribed as a part of a polycistronic mRNA
Products: -
Products: -
?
carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate
phosphate + a 3-carbamoyloxymethylcephem
-
Substrates: enzyme is involved in biosynthesis of cephamycin
Products: -
Products: -
?
carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate
phosphate + a 3-carbamoyloxymethylcephem
-
Substrates: catalyzes the synthesis of a wide range of carbamoyloxymethylcephems, e.g. cefuroxime
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate
phosphate + a 3-carbamoyloxymethylcephem
carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate
phosphate + a 3-carbamoyloxymethylcephem
-
Substrates: the cmcH gene - encoding 3-hydroxymethylcephem carbamoyltransferase - upstream region does not contain a functional promoter, suggesting that cmcH is transcribed as a part of a polycistronic mRNA
Products: -
Products: -
?
carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate
phosphate + a 3-carbamoyloxymethylcephem
-
Substrates: enzyme is involved in biosynthesis of cephamycin
Products: -
Products: -
?
carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate
phosphate + a 3-carbamoyloxymethylcephem
-
Substrates: catalyzes the synthesis of a wide range of carbamoyloxymethylcephems, e.g. cefuroxime
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ATP
-
activation, up to 1 mM, may be substrate or effector, maximal activity in the presence of Mg2+ and Mn2+
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
-
activation, maximal activity in the presence of Mn2+ and ATP
Mn2+
-
activation, maximal activity in the presence of Mg2+ and ATP
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A087S1D3_9ARCH
568
0
64676
TrEMBL
-
T0YZ14_9ZZZZ
568
0
64380
TrEMBL
other Location (Reliability: 1)
T0Z9H2_9ZZZZ
234
0
26606
TrEMBL
other Location (Reliability: 3)
A0A852TVA4_9ACTN
523
0
56800
TrEMBL
-
T1ALB9_9ZZZZ
95
0
10726
TrEMBL
other Location (Reliability: 2)
A0A0F3GQ26_9BACT
100
0
11846
TrEMBL
-
A0A0M9EBB2_9DELT
3218
0
366022
TrEMBL
-
A0A0N0D5K6_9DELT
604
0
69193
TrEMBL
-
T1AM37_9ZZZZ
52
0
5679
TrEMBL
other Location (Reliability: 3)
A0A0M9DZX7_9DELT
599
0
68302
TrEMBL
-
A0A0N0D239_9DELT
568
0
64837
TrEMBL
-
T1AVH3_9ZZZZ
362
0
41170
TrEMBL
other Location (Reliability: 1)
A0A0M9DW98_9DELT
621
0
71416
TrEMBL
-
T0ZMI2_9ZZZZ
208
0
23546
TrEMBL
Secretory Pathway (Reliability: 4)
A0A087S239_9ARCH
173
0
20198
TrEMBL
-
A0A081S7U0_9ARCH
173
0
20198
TrEMBL
-
A0A1L7F9J8_9PSEU
522
0
58101
TrEMBL
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, in the presence of ATP and carbamoyl phosphate, stable for at least 3 days
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Brewer, S.J.; Taylor, P.M.; Turner, M.K.
An adenosine triphosphate-dependent carbamoylphosphate--3-hydroxymethylcephem O-carbamoyltransferase from Streptomyces clavuligerus
Biochem. J.
185
555-564
1980
Streptomyces clavuligerus
brenda
Chary, V.K.; de la Fuente, J.L.; Liras, P.; Martin, J.F.
Amy as a reporter gene for promoter activity in Nocardia lactamdurans: comparison of promoters of the cephamycin cluster
Appl. Environ. Microbiol.
63
2977-2982
1997
Amycolatopsis lactamdurans
brenda
Coque, J.J.R.; Perez-Llarena, F.J.; Enguita, F.J.; Fuente, J.L.; Martin, J.F.; Liras, P.
Characterization of the cmcH genes of Nocardia lactamdurans and Streptomyces clavuligerus encoding a functional 3'-hydroxymethylcephem O-carbamoyltransferase for cephamycin biosynthesis
Gene
162
21-27
1995
Amycolatopsis lactamdurans, no activity in Streptomyces griseus, no activity in Streptomyces lipmanii, Streptomyces cattleya
brenda
EXTERNAL LINKS (specific for EC-Number 2.1.3.7)
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Release 2023.1 (January 2023)
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