We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 2.1.2.7 - D-alanine 2-hydroxymethyltransferase for references in articles please use BRENDA:EC2.1.2.7
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
mshmt, alpha-methylserine hydroxymethyltransferase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-methylserine hydroxymethyltransferase
-
-
-
-
alpha-methylserine hydroxymethyltransferase
hydroxymethyltransferase, 2-methylserine
-
-
-
-
alpha-methylserine hydroxymethyltransferase
-
-
-
-
alpha-methylserine hydroxymethyltransferase
B2DEU8
-
alpha-methylserine hydroxymethyltransferase
-
MSHMT
B2DEU8
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5,10-methylenetetrahydrofolate + D-alanine + H2O = tetrahydrofolate + 2-methylserine
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydroxymethyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5,10-methylenetetrahydrofolate:D-alanine 2-hydroxymethyltransferase
Also acts on 2-hydroxymethylserine.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-methyl-L-serine + tetrahydrofolate
D-alanine + 5,10-methylenetetrahydrofolate
tetrahydrofolate + (+)-2-methylserine
5,10-methylenetetrahydrofolate + D-alanine + H2O
tetrahydrofolate + (-)-ethylserine
5,10-methylenetetrahydrofolate + D-2-aminobutyrate + H2O
tetrahydrofolate + 2-hydroxymethylserine
5,10-methylenetetrahydrofolate + D-serine + H2O
2-methyl-L-serine + tetrahydrofolate
D-alanine + 5,10-methylenetetrahydrofolate
B2DEU8
assay at pH 7.4, 30°C
-
-
r
2-methyl-L-serine + tetrahydrofolate
D-alanine + 5,10-methylenetetrahydrofolate
assay at pH 7.4, 30°C
-
-
r
tetrahydrofolate + (+)-2-methylserine
5,10-methylenetetrahydrofolate + D-alanine + H2O
-
-
-
-
?
tetrahydrofolate + (+)-2-methylserine
5,10-methylenetetrahydrofolate + D-alanine + H2O
-
-
-
-
?
tetrahydrofolate + (+)-2-methylserine
5,10-methylenetetrahydrofolate + D-alanine + H2O
-
stereospecific, dimedon cannot replace tetrahydrofolate
-
r
tetrahydrofolate + (+)-2-methylserine
5,10-methylenetetrahydrofolate + D-alanine + H2O
-
stereospecific, dimedon cannot replace tetrahydrofolate
-
r
tetrahydrofolate + (+)-2-methylserine
5,10-methylenetetrahydrofolate + D-alanine + H2O
-
stereospecific, dimedon cannot replace tetrahydrofolate
5,10-methylenetetrahydrofolate is non-enzymatically cleaved to tetrahydrofolate and formaldehyde
r
tetrahydrofolate + (+)-2-methylserine
5,10-methylenetetrahydrofolate + D-alanine + H2O
-
stereospecific, dimedon cannot replace tetrahydrofolate
5,10-methylenetetrahydrofolate is non-enzymatically cleaved to tetrahydrofolate and formaldehyde
r
tetrahydrofolate + (-)-ethylserine
5,10-methylenetetrahydrofolate + D-2-aminobutyrate + H2O
-
-
-
r
tetrahydrofolate + (-)-ethylserine
5,10-methylenetetrahydrofolate + D-2-aminobutyrate + H2O
-
-
-
r
tetrahydrofolate + (-)-ethylserine
5,10-methylenetetrahydrofolate + D-2-aminobutyrate + H2O
-
stereospecific
-
r
tetrahydrofolate + (-)-ethylserine
5,10-methylenetetrahydrofolate + D-2-aminobutyrate + H2O
-
-
-
r
tetrahydrofolate + 2-hydroxymethylserine
5,10-methylenetetrahydrofolate + D-serine + H2O
-
-
-
-
tetrahydrofolate + 2-hydroxymethylserine
5,10-methylenetetrahydrofolate + D-serine + H2O
-
specific removal of one of the two hydroxyl groups
-
r
tetrahydrofolate + 2-hydroxymethylserine
5,10-methylenetetrahydrofolate + D-serine + H2O
-
specific removal of one of the two hydroxyl groups
-
-
r
tetrahydrofolate + 2-hydroxymethylserine
5,10-methylenetetrahydrofolate + D-serine + H2O
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pyridoxal 5'-phosphate
-
Km: 0.01 mM at pH 7.5; requirement
pyridoxal 5'-phosphate
-
requirement
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Cu2+
-
at higher concentrations
DL-cycloserine
-
strong, competitive
Fe2+
-
at higher concentrations
Fe3+
-
at higher concentrations
L-serine
-
weak, competitive
p-chloromercuribenzoate
-
-
Zn2+
-
at higher concentrations
additional information
-
no inhibition by Mg2+, Mn2+, Al3+, 2-mercaptoethanol
-
additional information
-
iodoacetate, GSH; no inhibition by Mg2+, Mn2+, Al3+, 2-mercaptoethanol
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6.2
2-ethyl-DL-serine
-
pH 7.5
1.5
2-methyl-DL-serine
-
pH 7.5
1.5 - 1.88
2-methyl-L-serine
0.8
hydroxymethylserine
-
pH 7.5
0.25
L-tetrahydrofolate
-
pH 7.5, calculated on the basis of one isomer
0.09 - 0.228
tetrahydrofolate
1.5
2-methyl-L-serine
B2DEU8
-
0.09
tetrahydrofolate
B2DEU8
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.002 - 0.0022
D-cycloserine
0.002
D-cycloserine
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
B2DEU8
4.75 U/mg, cell-free extract; 8.32 U/mg, after purification
additional information
1.25 U/mg, cell-free extract; 7.10 U/mg, after purification
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.4
B2DEU8
assay at
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
6 - 10.5
-
about half-maximal activity at pH 6.0 and 10.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
30
B2DEU8
assay at
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
33 - 48
-
about half-maximal activity at 33°C and 90% activity at 48°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
B2DEU8
UniProt
brenda
-
-
-
brenda
-
UniProt
brenda
-
-
-
brenda
inducible enzyme
-
-
brenda
-
-
-
brenda
inducible enzyme
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
B2DEV1_9RHIZ
425
0
45600
TrEMBL
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
73000
B2DEU8
gel filtration
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
homodimer
B2DEU8
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
45
-
96% loss of activity after 10 min, hydroxymethylserine plus tetrahydrofolate and pyridoxal 5'-phosphate prevent
50
B2DEU8
value about
50
-
complete inactivation after 10 min, 55% loss of activity in the presence of tetrahydrofolate, pyridoxal 5'-phosphate plus hydroxymethylserine or D-serine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
prolonged dialysis inactivates
-
pyridoxal 5-phosphate restores activity
-
pyridoxal 5-phosphate stabilizes during purification
-
tetrahydrofolate plus pyridoxal 5-phosphate plus hydroxymethylserine increase temperature-stability
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
column chromatography, 16.4% yield
column chromatography, 72.8% yield
B2DEU8
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Escherichia coli
expression in Escherichia coli
B2DEU8
expression in Escherichia coli
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Wilson Miles, E.
alpha-Methylserine transhydroxymethylase (Pseudomonas)
Methods Enzymol.
17B
341-346
1971
Pseudomonas sp.
-
brenda
Wilson, E.M.; Snell, E.E.
Metabolism of alpha-methylserine. I. Alpha-methylserine hydroxymethyltransferase
J. Biol. Chem.
237
3171-3179
1962
Pseudomonas sp., Pseudomonas sp. MS
brenda
Wilson, E.M.; Snell, E.E.
Metabolism of alpha-methylserine. II. Stereospecificity of alpha-methylserine hydroxymethyltransferase
J. Biol. Chem.
237
3180-3184
1962
Pseudomonas sp.
brenda
Sperl, G.T.
Microbial metabolism of 2-methyl amino acids
Curr. Microbiol.
19
135-138
1989
Clostridium tetanomorphum, Escherichia coli
-
brenda
Nozaki, H.; Kuroda, S.; Watanabe, K.; Yokozeki, K.
Cloning of the gene encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and characterization of the recombinant enzyme
Biosci. Biotechnol. Biochem.
72
3002-3005
2008
Aminobacter sp. AJ110403 (B2DEU8), Ensifer sp. AJ110404 (B2DEV1)
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
2.1.2.7 D-alanine 2-hydroxymethyltransferase
more
top
Information
