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IUBMB CommentsThe enzyme, characterized from the bacterium Brucella melitensis, synthesizes a building block of the O antigen produced by Brucella species.
The expected taxonomic range for this enzyme is: Brucella
Synonyms
wbkC, more
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wbkC
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10-formyltetrahydrofolate + GDP-alpha-D-perosamine = tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
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MetaCyc
GDP-N-formyl-alpha-D-perosamine biosynthesis
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10-formyltetrahydrofolate:GDP-alpha-D-perosamine N-formyltransferase
The enzyme, characterized from the bacterium Brucella melitensis, synthesizes a building block of the O antigen produced by Brucella species.
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10-formyltetrahydrofolate + GDP-3-deoxyperosamine
tetrahydrofolate + GDP-N-formyl-3-deoxyperosamine
Q9ZHX0
Substrates: -
Products: -
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10-formyltetrahydrofolate + GDP-alpha-D-perosamine
tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
10-formyltetrahydrofolate + GDP-alpha-D-perosamine
tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
Q9ZHX0
Substrates: -
Products: -
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10-formyltetrahydrofolate + GDP-alpha-D-perosamine
tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
Q9ZHX0
Substrates: -
Products: -
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10-formyltetrahydrofolate + GDP-alpha-D-perosamine
tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
10-formyltetrahydrofolate + GDP-alpha-D-perosamine
tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
Q9ZHX0
Substrates: -
Products: -
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10-formyltetrahydrofolate + GDP-alpha-D-perosamine
tetrahydrofolate + GDP-N-formyl-alpha-D-perosamine
Q9ZHX0
Substrates: -
Products: -
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GDP-alpha-D-perosamine
Q9ZHX0
substrate inhibition by GDP-alpha-D-perosamine is evident above 0.25 mM
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0.46
GDP-alpha-D-perosamine
Q9ZHX0
mutant enzyme D78A/F142A, at pH 8.0, temperature not specified in the publication
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0.54
GDP-alpha-D-perosamine
Q9ZHX0
mutant enzyme D78A/F142A, at pH 8.0, temperature not specified in the publication
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1.2
GDP-alpha-D-perosamine
Q9ZHX0
mutant enzyme D78A/F142A, at pH 8.0, temperature not specified in the publication
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Q9ZHX0
UniProt
brenda
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Q9ZHX0
UniProt
brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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physiological function
Q9ZHX0
the enzyme is absolutely required for the O-side-chain production of the lipopolysaccharide O-antigen
physiological function
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the enzyme is absolutely required for the O-side-chain production of the lipopolysaccharide O-antigen
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WBKC_BRUME
Brucella melitensis biotype 1 (strain ATCC 23456 / CCUG 17765 / NCTC 10094 / 16M)
259
0
29128
Swiss-Prot
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WBKC_BRUA2
Brucella abortus (strain 2308)
259
0
29128
Swiss-Prot
other Location (Reliability: 1)
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Q9ZHX0
x * 29100, calculated from amino acid sequence
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x * 29100, calculated from amino acid sequence
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mutant enzyme D78A/F142A, hanging drop vapor diffusion method, using 13-15% (w/v) poly(ethylene glycol) 5000, 2% (v/v) glycerol. Wild type enzyme, hanging drop vapor diffusion method, using 12-15% (w/v) poly(ethylene glycol) 8000m and 2% (w/v) 2-methyl-2,4-pentanediol
Q9ZHX0
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D78A/F142A
Q9ZHX0
the mutant variant does not exhibit substrate inhibition, but does display kinetic behavior expected for an enzyme with modest cooperativity
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Ni-NTA resin column chromatography
Q9ZHX0
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expressed in Escherichia coli Rosetta (DE3) cells
Q9ZHX0
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Riegert, A.S.; Chantigian, D.P.; Thoden, J.B.; Tipton, P.A.; Holden, H.M.
Biochemical Characterization of WbkC, an N-Formyltransferase from Brucella melitensis
Biochemistry
56
3657-3668
2017
Brucella melitensis (Q9ZHX0)
brenda
Godfroid, F.; Cloeckaert, A.; Taminiau, B.; Danese, I.; Tibor, A.; De Bolle, X.; Mertens, P.; Letesson, J.
Genetic organisation of the lipopolysaccharide O-antigen biosynthesis region of Brucella melitensis 16M (wbk)
Res. Microbiol.
151
655-668
2000
Brucella melitensis (Q9ZHX0), Brucella melitensis 16M (Q9ZHX0)
brenda
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