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EC Tree
The expected taxonomic range for this enzyme is: Homo sapiens
Reaction Schemes
a 5'-(5'-phospho)-tRNAHis
+
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a 5'-(5'-phosphomethyl)-tRNAHis
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Synonyms
bcdin3d, trnahis-specific 5'-monomethylphosphate capping enzyme,
more
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tRNAHis-specific 5'-monomethylphosphate capping enzyme
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a 5'-(5'-phospho)-tRNAHis + S-adenosyl-L-methionine = a 5'-(5'-phosphomethyl)-tRNAHis + S-adenosyl-L-homocysteine
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S-adenosyl-L-methionine:tRNAHis-specific 5'-phosphate monomethyltransferase
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tRNAHis + S-adenosyl-L-methionine
methylated tRNAHis + S-adenosyl-L-homocysteine
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additional information
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additional information
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the enzyme cannot methylate a tRNAHis mutant with a 5'-triphosphate group at its 5'-end in vitro
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additional information
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the enzyme cannot methylate a tRNAHis mutant with a 5'-triphosphate group at its 5'-end in vitro
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tRNAHis + S-adenosyl-L-methionine
methylated tRNAHis + S-adenosyl-L-homocysteine
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Mg2+
5 mM used in assay conditions
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Breast Neoplasms
BCDIN3D RNA methyltransferase stimulates Aldolase C expression and glycolysis through let-7 microRNA in breast cancer cells.
Breast Neoplasms
Crystal structure of human cytoplasmic tRNAHis-specific 5'-monomethylphosphate capping enzyme.
Breast Neoplasms
Elevated expression of RNA methyltransferase BCDIN3D predicts poor prognosis in breast cancer.
Breast Neoplasms
Human BCDIN3D Is a Cytoplasmic tRNAHis-Specific 5'-Monophosphate Methyltransferase.
Breast Neoplasms
Human BCDIN3D monomethylates cytoplasmic histidine transfer RNA.
Breast Neoplasms
Human RNA methyltransferase BCDIN3D regulates microRNA processing.
Breast Neoplasms
RNA methyltransferase BCDIN3D is crucial for female fertility and miRNA and mRNA profiles in Drosophila ovaries.
Infertility, Female
RNA methyltransferase BCDIN3D is crucial for female fertility and miRNA and mRNA profiles in Drosophila ovaries.
Neoplasms
BCDIN3D RNA methyltransferase stimulates Aldolase C expression and glycolysis through let-7 microRNA in breast cancer cells.
Neoplasms
Crystal structure of human cytoplasmic tRNAHis-specific 5'-monomethylphosphate capping enzyme.
Neoplasms
Elevated expression of RNA methyltransferase BCDIN3D predicts poor prognosis in breast cancer.
Neoplasms
Human BCDIN3D Is a Cytoplasmic tRNAHis-Specific 5'-Monophosphate Methyltransferase.
Obesity
BCDIN3D RNA methyltransferase stimulates Aldolase C expression and glycolysis through let-7 microRNA in breast cancer cells.
Triple Negative Breast Neoplasms
Elevated expression of RNA methyltransferase BCDIN3D predicts poor prognosis in breast cancer.
Uterine Cervical Neoplasms
MiR-195-3p inhibits cell proliferation in cervical cancer by targeting BCDIN3D.
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UniProt
brenda
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brenda
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BN3D2_HUMAN
292
0
33200
Swiss-Prot
other Location (Reliability: 3)
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sitting drop vapor diffusion method, using 20% (w/v) PEG 3350 and 200 mM CaCl2
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D110A
the mutant shows severely reduced activity compared to the wild type enzyme
D135A
the mutant shows severely reduced activity compared to the wild type enzyme
D72A
the mutant shows severely reduced activity compared to the wild type enzyme
D72A/G74A
the mutant shows severely reduced activity compared to the wild type enzyme
F136G
the mutant shows severely reduced activity compared to the wild type enzyme
G74A
the mutant shows severely reduced activity compared to the wild type enzyme
I111G
the mutant shows severely reduced activity compared to the wild type enzyme
I166G
the mutant shows severely reduced activity compared to the wild type enzyme
K201A
the mutant shows severely reduced activity compared to the wild type enzyme
N76A
the mutant shows severely reduced activity compared to the wild type enzyme
Q198A
the mutant shows severely reduced activity compared to the wild type enzyme
R118A
the mutant shows reduced activity compared to the wild type enzyme
R204A
the mutant shows severely reduced activity compared to the wild type enzyme
R208A
the mutant shows severely reduced activity compared to the wild type enzyme
R208A/R209A
the mutant shows severely reduced activity compared to the wild type enzyme
R211A
the mutant shows severely reduced activity compared to the wild type enzyme
R212A
the mutant shows severely reduced activity compared to the wild type enzyme
R257A
the mutant shows severely reduced activity compared to the wild type enzyme
R46A
the mutant shows severely reduced activity compared to the wild type enzyme
S165A
the mutant shows severely reduced activity compared to the wild type enzyme
W169F
the mutant shows severely reduced activity compared to the wild type enzyme
Y37A
the mutant shows severely reduced activity compared to the wild type enzyme
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Ni-NTA agarose column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration
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expressed in Escherichia coli BL21(DE3) cells
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Liu, Y.; Martinez, A.; Yamashita, S.; Tomita, K.
Crystal structure of human cytoplasmic tRNAHis-specific 5'-monomethylphosphate capping enzyme
Nucleic Acids Res.
48
1572-1582
2020
Homo sapiens (Q7Z5W3), Homo sapiens
brenda
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