Information on EC 2.1.1.B128 - 28S rRNA (adenine1322-N1)-methyltransferase

Substrates: 25S/28S rRNA of Homo sapiens contains a single m1A methylation in the helix 25.1. Human enzyme NML is able to methylate yeast 25S rRNA in vivo, cf. Ec 2.1.1.287
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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

S-adenosyl-L-methionine + adenine1322 in 28S rRNA

S-adenosyl-L-homocysteine + N1-methyladenine1322 in 28S rRNA

Substrates: -
Products: -

additional information

2 entries

additional information

Substrates: 25S/28S rRNA of Homo sapiens contains a single m1A methylation in the helix 25.1. Human enzyme NML is able to methylate yeast 25S rRNA in vivo, cf. Ec 2.1.1.287
Products: -

additional information

Substrates: 25S/28S rRNA of Homo sapiens contains a single m1A methylation in the helix 25.1. Human enzyme NML is able to methylate yeast 25S rRNA in vivo, cf. Ec 2.1.1.287
Products: -

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

S-adenosyl-L-methionine

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DISEASE

TITLE OF PUBLICATION

LINK TO PUBMED

28s rrna (adenine1322-n1)-methyltransferase deficiency

Nucleomethylin deficiency impairs embryonic erythropoiesis.

29244083

Starvation

A metabolic throttle regulates the epigenetic state of rDNA.

18485866

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

UniProt

brenda

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

brenda

show all columns Go to Localization Search

only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

nucleolus

NML is primarily localized in the nucleolus

brenda

brenda

Highest Expressing Human Cell Lines

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Gene Links

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

evolution

the N1-methyladenosine in helix 25.1 of 25/28S rRNA seems to be highly conserved

malfunction

siRNA-mediated depletion of NML is highly efficient at mRNA level, impact of an NML knockdown on m1A1322 methylation in HCT-116 cells. Depletion of NML leads to abnormal and altered nucleolar morphology

physiological function

the human nucleomethylin (NML or RRP8) fails to complement the pre-rRNA processing phenotype and the growth cold sensitivity of Saccharomyces cerevisiae DELTArrp8 mutant strain

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

O43159 pBLAST

RRP8_HUMAN

456

50715

Swiss-Prot

Show Sequence

other Location (Reliability: 2)

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

additional information

2 entries

additional information

siRNA-mediated depletion of NML in HCT-116 cells, impact of an NML knockdown on m1A1322 methylation in HCT-116 cells

additional information

siRNA-mediated depletion of NML in HCT-116 cells, impact of an NML knockdown on m1A1322 methylation in HCT-116 cells

Go to Cloned (commentary) Search

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

gene rrp8, sequence comparisons and phylogenetic analysis, quantitative enzyme expression analysis, recombinant expression in Saccharomyces cereviaie Rrp8 deficient mutant strain without complementation by the human Rrp8 enzyme

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE