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EC unknown
Reaction Schemes
+
adenine37 in tRNA
=
+
2-methyladenine37 in tRNA
Synonyms
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S-adenosyl-L-methionine + adenine37 in tRNA = S-adenosyl-L-homocysteine + 2-methyladenine37 in tRNA
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S-adenosyl-L-methionine:tRNA (adenine37)-methyltransferase
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S-adenosyl-L-methionine + adenine37 in Escherichia coli tRNAArgACG
S-adenosyl-L-homocysteine + methyladenine37 in Escherichia coli tRNAArgACG
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Substrates: -
?
S-adenosyl-L-methionine + adenine37 in Escherichia coli tRNAAspGUC
S-adenosyl-L-homocysteine + methyladenine37 in Escherichia coli tRNAAspGUC
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Substrates: -
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S-adenosyl-L-methionine + adenine37 in Escherichia coli tRNAGlnCUG
S-adenosyl-L-homocysteine + methyladenine37 in Escherichia coli tRNAGlnCUG
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Substrates: -
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S-adenosyl-L-methionine + adenine37 in Escherichia coli tRNAGlnUUG
S-adenosyl-L-homocysteine + methyladenine37 in Escherichia coli tRNAGlnUUG
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Substrates: -
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S-adenosyl-L-methionine + adenine37 in Escherichia coli tRNAGluUUC
S-adenosyl-L-homocysteine + methyladenine37 in Escherichia coli tRNAGluUUC
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Substrates: -
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S-adenosyl-L-methionine + adenine37 in Escherichia coli tRNAHisGUG
S-adenosyl-L-homocysteine + methyladenine37 in Escherichia coli tRNAHisGUG
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Substrates: -
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S-adenosyl-L-methionine + adenine37 in tRNAChimeraUUG
S-adenosyl-L-homocysteine + 2-methyladenine37 in tRNAChimeraUUG
additional information
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S-adenosyl-L-methionine + adenine37 in tRNAChimeraUUG
S-adenosyl-L-homocysteine + 2-methyladenine37 in tRNAChimeraUUG
Substrates: the anticodon stem-loop of the tRNA scaffold in the substrate is replaced with the anticodon stem-loop of tRNAGlncmnm5s2UUG
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S-adenosyl-L-methionine + adenine37 in tRNAChimeraUUG
S-adenosyl-L-homocysteine + 2-methyladenine37 in tRNAChimeraUUG
Substrates: -
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additional information
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Substrates: no substrate: adenine 37 in tRNAGlyCCC. Position 38 of tRNAs as a critical determinant of substrate recognition. tRNA methylation requirements are consistent with radical SAM reactivity
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additional information
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Substrates: residue U35 and G36 are imprtant for recognition by NmlA
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S-adenosyl-L-methionine + adenine37 in Escherichia coli tRNAArgACG
S-adenosyl-L-homocysteine + methyladenine37 in Escherichia coli tRNAArgACG
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Substrates: -
?
S-adenosyl-L-methionine + adenine37 in Escherichia coli tRNAAspGUC
S-adenosyl-L-homocysteine + methyladenine37 in Escherichia coli tRNAAspGUC
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Substrates: -
?
S-adenosyl-L-methionine + adenine37 in Escherichia coli tRNAGlnCUG
S-adenosyl-L-homocysteine + methyladenine37 in Escherichia coli tRNAGlnCUG
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Substrates: -
?
S-adenosyl-L-methionine + adenine37 in Escherichia coli tRNAGlnUUG
S-adenosyl-L-homocysteine + methyladenine37 in Escherichia coli tRNAGlnUUG
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Substrates: -
?
S-adenosyl-L-methionine + adenine37 in Escherichia coli tRNAGluUUC
S-adenosyl-L-homocysteine + methyladenine37 in Escherichia coli tRNAGluUUC
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Substrates: -
?
S-adenosyl-L-methionine + adenine37 in Escherichia coli tRNAHisGUG
S-adenosyl-L-homocysteine + methyladenine37 in Escherichia coli tRNAHisGUG
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Substrates: -
?
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4Fe-4S-center
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required for methylation of tRNA
NADPH
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or FldA/Fpr, required for methylation of tRNA
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UniProt
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UniProt
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UniProt
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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physiological function
RlmN is a dual-specificity enzyme that catalyzes methylation of both rRNA and tRNA. The RlmN mutant lacks 2-methyladenine in both tRNA set and in the peptidyl transferase center of 23S RNA. RlmN works in a late step during tRNA maturation by recognizing a precise 3D structure of tRNA. RlmN inactivation increases the misreading of a UAG stop codon
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RLMN_ECO24
Escherichia coli O139:H28 (strain E24377A / ETEC)
384
0
43086
Swiss-Prot
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RLMN_ECOLI
Escherichia coli (strain K12)
384
0
43086
Swiss-Prot
Secretory Pathway (Reliability: 3 )
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QM/MM calculations. Cross-linked RlmN-tRNA intermediate IM2 is formed by the attack of the C355-based methylene radical on the sp2-hybridized C2 of the adenosine ring, corresponding to an energy barrier of 14.4 kcal/mol, and the resolution of IM2 is confirmed to follow a radical fragmentation mechanism. The cleavage of the C'-S' bond of mC355-A37 cross-link is in concert with the deprotonation of C2 by residue C118, which is the rate-limiting step with an energy barrier of 17.4 kcal/mol. The cleavage of the C'-S' bond of IM2 can occur independently, that is, it does not require the loss of an electron of IM2 and the formation of disulfide bond between C355 and C118 as precondition
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C118A
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loss of tRNA methylation activity
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Benitez-Paez, A.; Villarroya, M.; Armengod, M.E.
The Escherichia coli RlmN methyltransferase is a dual-specificity enzyme that modifies both rRNA and tRNA and controls translational accuracy
RNA
18
1783-1795
2012
Escherichia coli (P36979)
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Fitzsimmons, C.M.; Fujimori, D.G.
Determinants of tRNA recognition by the radical SAM enzyme RlmN
PLoS ONE
11
e0167298
2016
Escherichia coli
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Zhao, C.; Dong, L.; Liu, Y.
A QM/MM study of the catalytic mechanism of SAM methyltransferase RlmN from Escherichia coli
Proteins
85
1967-1974
2017
Escherichia coli (A7ZPW0), Escherichia coli O139:H28 (A7ZPW0)
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