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EC unknown
Reaction Schemes
+
DNA containing the recognition sequence 5'-GTAC-3'
=
+
DNA containing the methylated recognition sequence 5'-GTm6AC-3'
Synonyms
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5'-GTAC-3'N6-adenine methyltransferase
5'-GTAC-3'N6-adenine methyltransferase
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5'-GTAC-3'N6-adenine methyltransferase
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-
M.PabI
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M.PabI
PabI constitutes a type II restriction-modification gene complex
M.PabI
PabI constitutes a type II restriction-modification gene complex
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PAB2246
locus name
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S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3' = S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3'
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-
-
-
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S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3'
S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3'
S-adenosyl-L-methionine + oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5'
S-adenosyl-L-homocysteine + oligoduplex 5'-GGACGCTTCACCGGATGTACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5'
S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3'
S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3'
Substrates: -
?
S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3'
S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3'
Substrates: -
?
S-adenosyl-L-methionine + oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5'
S-adenosyl-L-homocysteine + oligoduplex 5'-GGACGCTTCACCGGATGTACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5'
Substrates: -
?
S-adenosyl-L-methionine + oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5'
S-adenosyl-L-homocysteine + oligoduplex 5'-GGACGCTTCACCGGATGTACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5'
Substrates: -
?
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S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3'
S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3'
S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3'
S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3'
Substrates: -
?
S-adenosyl-L-methionine + DNA containing the recognition sequence 5'-GTAC-3'
S-adenosyl-L-homocysteine + DNA containing the methylated recognition sequence 5'-GTm6AC-3'
Substrates: -
?
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NaCl
activity is optimal at an NaCl concentration of 200 to 500 mM
additional information
no inhibition by Mg2+, Ca2+, or Mn2+
additional information
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no inhibition by Mg2+, Ca2+, or Mn2+
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Zn2+
50% inhibition observed at 0.3 mM
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0.000159
oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5'
pH 7.5, 75°C
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0.00128
S-adenosyl-L-methionine
pH 7.5, 75°C
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0.041
oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5'
pH 7.5, 75°C
-
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257
oligoduplex 5'-GGACGCTTCACCGGATGTm6ACAGGCATGCGACGACCCCTAG-3'/3'-CCTGCGAAGTGGCCTACATGTCCGTACGCTGCTGGGGATC-5'
pH 7.5, 75°C
-
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3 - 7.5
pH 3.0: about 55% of maximal activity, pH 7.5: about 50% of maximal activity
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65 - 95
65°C: about 40% of maximal activity, 95°C: about 40% of maximal activity
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UniProt
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UniProt
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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Q9V2B5_PYRAB
Pyrococcus abyssi (strain GE5 / Orsay)
464
0
52459
TrEMBL
Secretory Pathway (Reliability: 1 )
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52455
1 * 52455, calculated from sequence
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monomer
1 * 52455, calculated from sequence
monomer
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1 * 52455, calculated from sequence
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95
9 min, 50% of the activity is retained
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expression in Escherichia coli
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Watanabe, M.; Yuzawa, H.; Handa, N.; Kobayashi, I.
Hyperthermophilic DNA methyltransferase M.PabI from the archaeon Pyrococcus abyssi
Appl. Environ. Microbiol.
72
5367-5375
2006
Pyrococcus abyssi (Q9V2B5), Pyrococcus abyssi, Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 (Q9V2B5)
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