Information on EC 2.1.1.67 - thiopurine S-methyltransferase and Organism(s) Homo sapiens

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The taxonomic range for the selected organisms is: Homo sapiens

The enzyme appears in selected viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.67
-
RECOMMENDED NAME
GeneOntology No.
thiopurine S-methyltransferase
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Drug metabolism - other enzymes
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:thiopurine S-methyltransferase
Also acts, more slowly, on thiopyrimidines and aromatic thiols. Not identical with EC 2.1.1.9 thiol S-methyltransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
67339-09-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 2-aminothiophenol
S-adenosyl-L-homocysteine + 2-methylsulfanylphenylamine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 2-bromothiophenol
S-adenosyl-L-homocysteine + 1-bromo-2-methylsulfanylbenzene
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 2-mercaptoethanol
S-adenosyl-L-homocysteine + 2-methylmercaptoethanol
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 2-methoxythiophenol
S-adenosyl-L-homocysteine + 1-methoxy-2-methylsulfanylbenzene
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 2-thiouracil
S-adenosyl-L-homocysteine + thiouracil-2-S-methylether
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 3-methoxythiophenol
S-adenosyl-L-homocysteine + 1-methylsulfanyl-3-methoxybenzene
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 4-(aminomethoxy)thiophenol
S-adenosyl-L-homocysteine + 1-aminomethoxy-4-methylsulfanylbenzene
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 4-bromothiophenol
S-adenosyl-L-homocysteine + 1-bromo-4-methylsulfanylbenzene
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 4-chlorothiophenol
S-adenosyl-L-homocysteine + 1-chloro-4-methylsulfanylbenzene
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 4-fluorothiophenol
S-adenosyl-L-homocysteine + 1-fluoro-4-methylsulfanylbenzene
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 4-methoxythiophenol
S-adenosyl-L-homocysteine + 1-methoxy-4-methylsulfanylbenzene
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 4-methylthiophenol
S-adenosyl-L-homocysteine + 1-methyl-4-methylsulfanylbenzene
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 4-nitrothiophenol
S-adenosyl-L-homocysteine + 1-methylsulfanyl-4-nitrobenzene
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 4-thiobenzoate
S-adenosyl-L-homocysteine + 4-methylsulfanyl benzoate
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-hydroxy-8-mercaptopurine
S-adenosyl-L-homocysteine + 6-hydroxy-8-methylmercaptopurine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-mercaptopurine
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-mercaptopurine
S-adenosyl-L-homocysteine + 6-methylmercaptopurine
show the reaction diagram
S-adenosyl-L-methionine + 6-mercaptopurine nucleoside
S-adenosyl-L-homocysteine + 6-methylmercaptopurine nucleoside
show the reaction diagram
-
inactivation
-
-
?
S-adenosyl-L-methionine + 6-mercaptopurine-riboside
S-adenosyl-L-homocysteine + 6-methylmercaptopurine-riboside
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-mercaptopurine-riboside-5'-monophosphate
S-adenosyl-L-homocysteine + 6-methylmercaptopurine-riboside-5'-monophosphate
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-mercaptopurine-riboside-5'-triphosphate
S-adenosyl-L-homocysteine + 6-methylmercaptopurine-riboside-5'-triphosphate
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-selenoguanine-riboside
S-adenosyl-L-homocysteine + 6-methylselenoguanine-riboside
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-selenopurine
S-adenosyl-L-homocysteine + 6-methylselenopurine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-selenopurine-riboside
S-adenosyl-L-homocysteine + 6-methylselenopurine-riboside
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-thiodeoxyguanosine monophosphate
S-adenosyl-L-homocysteine + 6-methylthiodeoxyguanosine monophosphate
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-thiodeoxyinosine monophosphate
S-adenosyl-L-homocysteine + 6-methyl thiodeoxyinosine monophosphate
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-thioguanine
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-thioguanine
S-adenosyl-L-homocysteine + 6-methylthioguanine
show the reaction diagram
S-adenosyl-L-methionine + 6-thioguanine monophosphate
S-adenosyl-L-homocysteine + 6-methylthioguanine monophosphate
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-thioguanine-riboside
S-adenosyl-L-homocysteine + 6-methylthioguanine-riboside
show the reaction diagram
S-adenosyl-L-methionine + 6-thioguanine-riboside-5'-monophosphate
S-adenosyl-L-homocysteine + 6-methylthioguanine-riboside-5'-monophosphate
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-thioguanosine monophosphate
S-adenosyl-L-homocysteine + 6-methyl thioguanosine monophosphate
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-thioinosine
S-adenosyl-L-homocysteine + 6-methylmercaptopurine riboside
show the reaction diagram
S-adenosyl-L-methionine + 6-thioinosine 5'-monophosphate
S-adenosyl-L-homocysteine + 6-methylmercaptopurine ribonucleotide
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-thioinosine monophosphate
S-adenosyl-L-homocysteine + 6-methyl thioinosine monophosphate
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-thioinosine monophosphate
S-adenosyl-L-homocysteine + 6-methylthioinosine monophosphate
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-thioinosine-monophosphate
S-adenosyl-L-homocysteine + 6-methylmercaptopurine-ribonucleotides
show the reaction diagram
-
-
consisting of 6-methyl-thioinosine-monophosphate, -diphosphate and triphosphate
-
?
S-adenosyl-L-methionine + 6-thiopurine
S-adenosyl-L-homocysteine + 6-methylthiopurine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 7-methyl-6-mercaptopurine
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 8-hydroxy-6-mercaptopurine
S-adenosyl-L-homocysteine + 8-hydroxy-6-methylmercaptopurine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 9(n-propyl)6-thioguanine
S-adenosyl-L-homocysteine + 9(n-propyl)6-methylthioguanine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 9-(n-butyl)-6-mercaptopurine
S-adenosyl-L-homocysteine + 9-(n-butyl)-6-methylmercaptopurine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 9-ethyl-6-mercaptopurine
S-adenosyl-L-homocysteine + 9-ethyl-6-methylmercaptopurine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + a thiopurine
S-adenosyl-L-homocysteine + a thiopurine S-methylether
show the reaction diagram
S-adenosyl-L-methionine + azathioprine
?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + azathioprine
S-adenosyl-L-homocysteine + 6-methylmercaptopurine + ?
show the reaction diagram
S-adenosyl-L-methionine + mercaptopurine
S-adenosyl-L-homocysteine + methylmercaptopurine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + thioguanine nucleotide
S-adenosyl-L-homocysteine + methylthioguanine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + thioinosine monophosphate
S-adenosyl-L-homocysteine + methylthioinosine monophosphate
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + thioinosine triphosphate
S-adenosyl-L-homocysteine + methylthioinosine triphosphate
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + thiopurine
S-adenosyl-L-homocysteine + methylthiopurine
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 6-mercaptopurine
S-adenosyl-L-homocysteine + 6-methylmercaptopurine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + 6-thiopurine
S-adenosyl-L-homocysteine + 6-methylthiopurine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + a thiopurine
S-adenosyl-L-homocysteine + a thiopurine S-methylether
show the reaction diagram
additional information
?
-
-
S-adenosyl-L-methionine and sinefungin prevent degradation of TPMT by stabilizing ist native structure
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
baseline activity 102%
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,8-dihydroxy-6-mercaptopurine
-
-
2-Hydroxy-6-mercaptopurine
-
-
3,4,5-triiodobenzoic acid
-
-
3,4-dimethoxy-5-hydroxybenzoic acid
-
-
3,4-dimethoxybenzoic acid
-
-
3,5-dichlorobenzoic acid
-
-
3,5-dihydroxy-4-methoxybenzoic acid
-
-
3,5-dimethylbenzoic acid
-
-
3,5-dinitrobenzoic acid
-
-
3-bromo-5-hydroxy-4-methoxybenzoic acid
-
-
3-chloro-5-hydroxy-4-methoxybenzoic acid
-
-
3-hydroxy-4,5-dimethoxybenzoic acid
-
-
3-hydroxy-4-methoxy-5-(dimethylamino)benzoic acid
-
-
3-hydroxy-4-methoxy-5-nitrobenzoic acid
-
-
3-hydroxy-4-methoxybenzoic acid
-
-
3-hydroxy-5-iodo-4-methoxybenzoic acid
-
-
4-hydroxy-3-methoxybenzoic acid
-
-
5-amino-salicylic acid
-
-
5-Aminosalicylate
-
-
6-Mercaptopurine
6-methylmercaptopurine
-
-
6-Thioguanine
-
mixed inhibitor of 6-mercaptopurine methylation
Azathioprine
-
IC50: 0.43-0.532 mM
bendroflumethiazide
-
-
Benzoic acid
celecoxib
-
-
diclofenac
-
-
Ethacrynic acid
-
-
flurbiprofen
-
-
Furosemide
Ibuprofen
-
-
Ketoprofen
-
-
lornoxicam
-
-
Mefenamic acid
-
-
meloxicam
-
-
mesalazine
-
-
N-ethylmaleimide
-
-
nabumetone
-
-
naproxen
-
-
olsalazine
-
-
paracetamol
-
-
piretanide
-
IC50: 0.3-0.313 mM
piroxicam
-
-
S-adenosyl-L-homocysteine
-
-
sulfasalazine
-
-
Sulphasalazine
-
-
testosterone
-
; IC50: 0.03-0.072 mM
tolfenamic acid
-
-
trichlormethiazide
-
-
tropolone
-
-
additional information
-
low levels of methionine can result in low S-adenosyl-L-methionine levels and consequently decreased TPMT activity
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
-
S-adenosyl-L-methionine acts as a positive modulator of TPMT activity, the effect of S-adenosyl-L-methionine appears to be restricted to protein stabilisation rather than an increase of TPMT expression
SKF-525A
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
168
2-mercaptoethanol
-
-
2
2-Thiouracil
-
-
0.138
6-hydroxy-8-mercaptopurine
-
-
0.00068 - 0.99
6-Mercaptopurine
1.17
6-mercaptopurine-riboside
-
-
1.27
6-mercaptopurine-riboside-5'-monophosphate
-
-
0.89
6-mercaptopurine-riboside-5'-triphosphate
-
-
0.139
6-selenoguanine-riboside
-
-
0.0291
6-selenopurine
-
-
0.0518
6-selenopurine-riboside
-
-
0.1314
6-thiodeoxyguanosine
-
recombinant enzyme, expressed in yeast
0.0127
6-thiodeoxyinosine
-
recombinant enzyme, expressed in yeast
0.0137 - 1.45
6-Thioguanine
0.761
6-thioguanine-riboside
-
-
1.04
6-thioguanine-riboside-5'-monophosphate
-
-
-
0.0261
6-thioguanosine
-
recombinant enzyme, expressed in yeast
0.0271
6-thioguanosine 5-monophosphate
-
recombinant enzyme, expressed in yeast
0.0551
6-thioinosine
-
recombinant enzyme, expressed in yeast
0.0257
6-thioinosine 5'-monophosphate
-
recombinant enzyme, expressed in yeast
0.231
7-methyl-6-mercaptourine
-
-
-
0.0961
8-hydroxy-6-mercaptopurine
-
-
0.292
9-(n-butyl)6-mercaptopurine
-
-
0.159
9-(n-propyl)-6-thioguanine
-
-
0.372
9-ethyl-6-mercaptopurine
-
-
0.0024 - 0.0463
S-adenosyl-L-methionine
additional information
additional information
-
various thiophenols as substrates, Km 0.0008-0.0078
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.027
2-Hydroxy-6-mercaptopurine
-
-
0.01 - 0.0113
3,4-dimethoxy-5-hydroxybenzoic acid
0.00802 - 0.0113
6-Mercaptopurine
0.45 - 0.9
6-Thioguanine
2.413
celecoxib
-
-
0.722
diclofenac
-
-
1.524
flurbiprofen
-
-
1.043
Ibuprofen
-
-
0.172
Ketoprofen
-
-
1.41
lornoxicam
-
-
0.039
Mefenamic acid
-
-
4.238
meloxicam
-
-
4.3
nabumetone
-
-
0.052
naproxen
-
-
0.208
olsalazine
-
-
5.162
paracetamol
-
-
2.589
piroxicam
-
-
0.00075
S-adenosyl-L-homocysteine
-
-
0.05
tolfenamic acid
-
-
0.85
tropolone
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.43 - 0.532
Azathioprine
Homo sapiens;
-
IC50: 0.43-0.532 mM
2.416
celecoxib
Homo sapiens;
-
-
1.582
diclofenac
Homo sapiens;
-
-
1.649
flurbiprofen
Homo sapiens;
-
-
0.015 - 0.019
Furosemide
Homo sapiens;
-
IC50: 0.015-0.019 mM, has the potential to inhibit thiopurine S-methyltransferase in patients with chronic inflammatory bowel disease
1.968
Ibuprofen
Homo sapiens;
-
-
1.013
Ketoprofen
Homo sapiens;
-
-
2.135
lornoxicam
Homo sapiens;
-
-
0.039
Mefenamic acid
Homo sapiens;
-
-
4.292
meloxicam
Homo sapiens;
-
-
4.341
nabumetone
Homo sapiens;
-
-
0.079
naproxen
Homo sapiens;
-
-
1.474
olsalazine
Homo sapiens;
-
-
5.168
paracetamol
Homo sapiens;
-
-
0.3 - 0.313
piretanide
Homo sapiens;
-
IC50: 0.3-0.313 mM
2.589
piroxicam
Homo sapiens;
-
-
0.03 - 0.072
testosterone
Homo sapiens;
-
IC50: 0.03-0.072 mM
0.063
tolfenamic acid
Homo sapiens;
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000716
-
TMPT reference activity after recovery of the bone marrow suppression
0.00003033
-
elevated TPMT activity during the recovery phase of a bone marrow suppression, following the pancytopenic period
1.03
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
TPMT activity assay
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 8.6
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
embryonic kidney cell line with inducible thiopurine methyltransferase
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Homo sapiens;
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
2 isozymes, gel filtration
33000
-
determined by SDS-PAGE and immunoblotting
35000
-
x * 35000, SDS-PAGE
36000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 35000, SDS-PAGE
monomer
-
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
the melting temperature of the wild type enzyme is at 55°C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine is responsible for direct stabilization of the enzyme
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
80°C, activity in cell lysate is stable during storage
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 isozymes
-
cytosolic fractions of transformed Saccharomyces cerevisiae cells are prepared
-
Ni-NTA column chromatography and Superdex 200 gel filtration
-
using a Ni2+-NTA resin and a gel-filtration column
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
baculovirus expressed
-
cDNA cloning, heterologous expression in yeast
-
enhanced green fluorescent protein-tagged enzyme is expressed in Escherichia coli, Jurkat, Hep-G2, and HEK-293 cells
-
expressed in COS-1 cells
-
expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells
-
for cloning of the TPMT fragment the TOPO TA cloning kit is used
-
into the pCR2.1-TOPO vector, used as a template to generate point mutations, the variants are sequenced and cloned into the mammalian expression vector pCMV6-XL5, into the pENTR/D-TOPO vector and subsequently into the pcDNA-DEST40 vector
-
into the yeast expression vector pYeDP60 for transformation of Saccharomyces cerevisiae cells
-
retroviral gene transfer
-
the full-length coding region is amplified by PCR from a eukaryotic expression plasmid and cloned into the pET-28a vector for expression in Escherichia coli BL21DE3 cells
-
to assess the functional effect of the trinucleotide repeat variants in the promoter, the wild-type and variant TPMT promoters are amplified and cloned into the promoter-less pGL3-Basic vector
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
azathioprine therapy can raise TPMT activity
-
decreased activity of TPMT is associated with hematopoietic toxicity after administration of standard doses of 6- mercaptopurine or azathioprine
-
longitudinal induction of thiopurine methyltransferase activity is observed during 6-thioguanine treatment
-
S-adenosyl-L-methionine has no effect on TPMT mRNA expression
-
smokers have significantly higher TPMT activity than nonsmokers
-
the restriction of L-methionine in cell growth media reversibly decreases enzyme activity and protein levels
-
TPMT activity is significantly higher in wild type children than in wild type adults (18-68 years), wild type infants from 0.08-5 years has a 9% higher average TPMT activity than the other wild type groups, only in children from 0.08-2 years is the TPMT activity higher in males than in females
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
238G>C
460G>A
460G>A/719A>G
-
TPMT*3A polymorphism, low or no TPMT activity
539A>T
-
TPMT variant
719A>G
A154T/Y240C
A154Y
-
thiopurine S-methyltransferase polymorphism, G to A transition at position 460
A179G/G460A
-
the variant is associated with low enzyme activity
A180P
-
the mutation affects TMPT activity
C216T
-
the mutant exhibits reduced activity compared to the wild type enzyme
E98X
-
the mutation affects TMPT activity
F208L
-
the mutation is associated with a decrease in enzyme activity
Q179H
-
polymorphism c.537G>T, TPMT*24
R152A
-
mutant to probe, whether this residue is important for catalysis
R152A/R226A
-
mutant to probe, whether this residue is important for catalysis
R152E
-
mutant to probe, whether this residue is important for catalysis
R152H
-
mutant to probe, whether this residue is important for catalysis
R226A
-
mutant to probe, whether this residue is important for catalysis
R226E
-
mutant to probe, whether this residue is important for catalysis
R226H
-
mutant to probe, whether this residue is important for catalysis
TPMT*2
TPMT*23
-
polymorphism, single nucleotide substitution, C500G
TPMT*3A
TPMT*3B
TPMT*3C
Y107D
-
the mutant shows less than 10% activity compared to the wild type enzyme, the variant allozyme shows a striking decrease in both immunoreactive protein level and enzyme activity after transient expression in COS-1 cells, the mutant is less stable than the wild type TPMT allozyme
additional information
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