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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
adenine-n6 methyltransferase, yfic protein, more
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adenine-N6 methyltransferase
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S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N6-methyladenine
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methyl group transfer
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S-adenosyl-L-methionine:tRNA (adenine-N6-)-methyltransferase
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S-adenosyl-L-methionine + adenine 37 in tRNA1Val(cmo5UAC)
S-adenosyl-L-homocysteine + N6-methyladenine 37 in tRNA1Val(cmo5UAC)
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Substrates: the enzyme specifically methylates adenosine at position 37 of tRNA1Val(cmo5UAC)
Products: -
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S-adenosyl-L-methionine + adenine in tRNA
S-adenosyl-L-homocysteine + N6-methyladenine in tRNA
S-adenosyl-L-methionine + adenine37 in tRNAGln
S-adenosyl-L-homocysteine + N6-methyladenine37 in tRNAGln
Substrates: i.e. tRNAGln from Mycoplasma capricolum
Products: -
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S-adenosyl-L-methionine + adenine37 in tRNALeu
S-adenosyl-L-homocysteine + N6-methyladenine37 in tRNALeu
Substrates: i.e. tRNALeu from Mycoplasma capricolum
Products: -
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S-adenosyl-L-methionine + adenine in tRNA
S-adenosyl-L-homocysteine + N6-methyladenine in tRNA
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Substrates: methyl group acceptor is yeast tRNA, poor acceptors are E. coli or mycoplasma tRNAs
Products: -
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S-adenosyl-L-methionine + adenine in tRNA
S-adenosyl-L-homocysteine + N6-methyladenine in tRNA
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Substrates: -
Products: -
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S-adenosyl-L-methionine + adenine in tRNA
S-adenosyl-L-homocysteine + N6-methyladenine in tRNA
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Substrates: -
Products: -
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Ca2+
TrmM is most active with Mg2+, Mn2+, Ca2+, or Fe2+
Fe2+
TrmM is most active with Mg2+, Mn2+, Ca2+, or Fe2+
Mg2+
TrmM is most active with Mg2+, Mn2+, Ca2+, or Fe2+
Mn2+
TrmM is most active with Mg2+, Mn2+, Ca2+, or Fe2+
additional information
divalent cation required, no activity with Fe3+ or Zn2+
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strains BW25141 and JW2559
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brenda
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UniProt
brenda
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brenda
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brenda
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brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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physiological function
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the yfiC gene confers a growth advantage under conditions of osmotic and oxidative stress
physiological function
expression of TrmM can substitute YfiC in Escherichia coli
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A0A0C2W699_MYCCA
240
0
27798
TrEMBL
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dimer
the dimeric form of TrmM is important for efficient tRNA binding and catalysis
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structures of TrmM with and without product S-adenosyl-L-homocysteine, to 2.16 and 2.52 A resolution, respectively
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H140A
10-50% of wild-type activity
H167A
10-50% of wild-type activity
K121A
10-50% of wild-type activity
K127A
10-50% of wild-type activity
K158A/K160A
about 10% of wild-type activity
R171A
10-50% of wild-type activity
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Ni-NTA agarose column chromatography
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expressed in Escherichia coli AG1 cells
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expression in Escherichia coli
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Sharma, O.K.
Differences in the transfer RNA methyltransferases from normal rat liver and Novikoff hepatoma
Biochim. Biophys. Acta
299
415-427
1973
Rattus norvegicus
brenda
Golovina, A.Y.; Sergiev, P.V.; Golovin, A.V.; Serebryakova, M.V.; Demina, I.; Govorun, V.M.; Dontsova, O.A.
The yfiC gene of E. coli encodes an adenine-N6 methyltransferase that specifically modifies A37 of tRNA1Val(cmo5UAC)
RNA
15
1134-1141
2009
Escherichia coli
brenda
Jeong, H.; Lee, Y.; Kim, J.
Structural and functional characterization of TrmM in m6A modification of bacterial tRNA
Protein Sci.
31
e4319
2022
Mycoplasma capricolum subsp. capricolum (A0A0C2W699)
brenda
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