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IUBMB CommentsIsolated from the fungus Psilocybe cubensis. The product, psilocybin, is a psychoactive compound.
The expected taxonomic range for this enzyme is: Psilocybe
Synonyms
PsiM, more
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PsiM
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2 S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate = 2 S-adenosyl-L-homocysteine + psilocybin
overall reaction
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S-adenosyl-L-methionine + 4-hydroxy-N-methyltryptamine 4-phosphate = S-adenosyl-L-homocysteine + psilocybin
(1b)
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S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate = S-adenosyl-L-homocysteine + 4-hydroxy-N-methyltryptamine 4-phosphate
(1a)
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S-adenosyl-L-methionine:4-hydroxytryptamine-4-phosphate N,N-dimethyltransferase
Isolated from the fungus Psilocybe cubensis. The product, psilocybin, is a psychoactive compound.
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2 S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate
2 S-adenosyl-L-homocysteine + psilocybin
S-adenosyl-L-methionine + 4-hydroxy-N-methyltryptamine 4-phosphate
S-adenosyl-L-homocysteine + psilocybin
Substrates: -
Products: -
?
S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate
S-adenosyl-L-homocysteine + 4-hydroxy-N-methyltryptamine 4-phosphate
Substrates: -
Products: -
?
S-adenosyl-L-methionine + baeocystin
S-adenosyl-L-homocysteine + psilocybin
Substrates: -
Products: -
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S-adenosyl-L-methionine + norbaeocystin
S-adenosyl-L-homocysteine + baeocystin
Substrates: -
Products: -
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2 S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate
2 S-adenosyl-L-homocysteine + psilocybin
Substrates: -
Products: -
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2 S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate
2 S-adenosyl-L-homocysteine + psilocybin
Substrates: the enzyme is strictly specific. It cannot tolerate a 7-phosphoryloxy-substituted compounds as acceptor substrate
Products: -
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2 S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate
2 S-adenosyl-L-homocysteine + psilocybin
Substrates: -
Products: -
?
S-adenosyl-L-methionine + 4-hydroxy-N-methyltryptamine 4-phosphate
S-adenosyl-L-homocysteine + psilocybin
Substrates: -
Products: -
?
S-adenosyl-L-methionine + 4-hydroxytryptamine 4-phosphate
S-adenosyl-L-homocysteine + 4-hydroxy-N-methyltryptamine 4-phosphate
Substrates: -
Products: -
?
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UniProt
brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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metabolism
the enzyme is involved in psilocybin synthesis
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PSIM_PSICU
309
0
34434
Swiss-Prot
other Location (Reliability: 3)
PSIM_PSICY
309
0
34312
Swiss-Prot
other Location (Reliability: 2)
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?
x * 34500, calculated from amino acid sequence
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D161A
the variant is as active as the wild-type enzyme in in vitro assays
N183A
the variant is catalytically inactive
R75A
the variant is catalytically inactive
Y187A
the variant is catalytically inactive
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metal affinity column chromatography
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expressed in Escherichia coli KRX cells
expression in Escherichia coli
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Fricke, J.; Blei, F.; Hoffmeister, D.
Enzymatic synthesis of psilocybin
Angew. Chem. Int. Ed. Engl.
56
12352-12355
2017
Psilocybe cubensis (P0DPA9)
brenda
Blei, F.; Baldeweg, F.; Fricke, J.; Hoffmeister, D.
Biocatalytic production of psilocybin and derivatives in tryptophan synthase-enhanced reactions
Chemistry
24
10028-10031
2018
Psilocybe cubensis (P0DPA9)
brenda
Fricke, J.; Sherwood, A.; Kargbo, R.; Orry, A.; Blei, F.; Naschberger, A.; Rupp, B.; Hoffmeister, D.
Enzymatic route toward 6-methylated baeocystin and psilocybin
ChemBioChem
20
2824-2829
2019
Psilocybe cubensis (P0DPA9)
brenda
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