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IUBMB Comments The enzyme, characterized from the soil bacterium Streptomyces davawensis , catalyses the last two steps in the biosynthesis of the antibiotic roseoflavin.
Synonyms
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N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase
N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase
E7BBN5
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N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase
E7BBN5
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RosA
E7BBN5
rosA: gene name
RosA
E7BBN5
rosA: gene name
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2 S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = 2 S-adenosyl-L-homocysteine + roseoflavin
S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = S-adenosyl-L-homocysteine + 8-demethyl-8-(methylamino)riboflavin
S-adenosyl-L-methionine + 8-demethyl-8-(methylamino)riboflavin = S-adenosyl-L-homocysteine + roseoflavin
2 S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = 2 S-adenosyl-L-homocysteine + roseoflavin
roseoflavin, i.e. 1-deoxy-1-[8-(dimethylamino)-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl]pentitol, a two-step methylation reaction
E7BBN5
2 S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = 2 S-adenosyl-L-homocysteine + roseoflavin
roseoflavin, i.e. 1-deoxy-1-[8-(dimethylamino)-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl]pentitol, a two-step methylation reaction
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2 S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = 2 S-adenosyl-L-homocysteine + roseoflavin
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S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = S-adenosyl-L-homocysteine + 8-demethyl-8-(methylamino)riboflavin
1a
E7BBN5
S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = S-adenosyl-L-homocysteine + 8-demethyl-8-(methylamino)riboflavin
1a
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S-adenosyl-L-methionine + 8-amino-8-demethylriboflavin = S-adenosyl-L-homocysteine + 8-demethyl-8-(methylamino)riboflavin
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S-adenosyl-L-methionine + 8-demethyl-8-(methylamino)riboflavin = S-adenosyl-L-homocysteine + roseoflavin
1b
E7BBN5
S-adenosyl-L-methionine + 8-demethyl-8-(methylamino)riboflavin = S-adenosyl-L-homocysteine + roseoflavin
1b
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S-adenosyl-L-methionine + 8-demethyl-8-(methylamino)riboflavin = S-adenosyl-L-homocysteine + roseoflavin
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S-adenosyl-L-methionine:8-amino-8-demethylriboflavin N,N-dimethyltransferase
The enzyme, characterized from the soil bacterium Streptomyces davawensis, catalyses the last two steps in the biosynthesis of the antibiotic roseoflavin.
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8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine
roseoflavin + S-adenosyl-L-homocysteine
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
S-adenosyl-L-homocysteine + roseoflavin
additional information
?
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8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine
roseoflavin + S-adenosyl-L-homocysteine
E7BBN5
Substrates: -
ir
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine
roseoflavin + S-adenosyl-L-homocysteine
E7BBN5
Substrates: -
ir
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine
roseoflavin + S-adenosyl-L-homocysteine
E7BBN5
Substrates: -
ir
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine
roseoflavin + S-adenosyl-L-homocysteine
E7BBN5
Substrates: -
ir
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
S-adenosyl-L-homocysteine + roseoflavin
Substrates: -
?
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
S-adenosyl-L-homocysteine + roseoflavin
Substrates: -
?
additional information
?
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E7BBN5
Substrates: no activity with 8-hydroxy-8-demethyl-D-riboflavin
?
additional information
?
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Substrates: no activity with 8-hydroxy-8-demethyl-D-riboflavin
?
additional information
?
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E7BBN5
Substrates: no activity with 8-hydroxy-8-demethyl-D-riboflavin
?
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8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine
roseoflavin + S-adenosyl-L-homocysteine
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
S-adenosyl-L-homocysteine + roseoflavin
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine
roseoflavin + S-adenosyl-L-homocysteine
E7BBN5
Substrates: -
ir
8-amino-8-demethyl-D-riboflavin + S-adenosyl-L-methionine
roseoflavin + S-adenosyl-L-homocysteine
E7BBN5
Substrates: -
ir
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
S-adenosyl-L-homocysteine + roseoflavin
Substrates: -
?
S-adenosyl-L-methionine + 8-amino-8-demethyl-D-riboflavin
S-adenosyl-L-homocysteine + roseoflavin
Substrates: -
?
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S-adenosyl-L-methionine
E7BBN5
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S-adenosyl-L-methionine
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roseoflavin
competitive inhibition
S-adenosyl-L-homocysteine
competitive inhibition
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0.004 - 0.0577
8-amino-8-demethyl-D-riboflavin
0.0286 - 0.07
S-adenosyl-L-methionine
0.004
8-amino-8-demethyl-D-riboflavin
at pH 8.0 and 25°C
0.0577
8-amino-8-demethyl-D-riboflavin
E7BBN5
pH 8.0, 37°C
0.0286
S-adenosyl-L-methionine
E7BBN5
pH 8.0, 37°C
0.07
S-adenosyl-L-methionine
at pH 8.0 and 25°C
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0.001 - 0.37
8-amino-8-demethyl-D-riboflavin
0.001 - 0.24
S-adenosyl-L-methionine
0.001
8-amino-8-demethyl-D-riboflavin
at pH 8.0 and 25°C
0.37
8-amino-8-demethyl-D-riboflavin
E7BBN5
pH 8.0, 37°C
0.001
S-adenosyl-L-methionine
at pH 8.0 and 25°C
0.24
S-adenosyl-L-methionine
E7BBN5
pH 8.0, 37°C
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0.007
roseoflavin
at pH 8.0 and 25°C
0.027
S-adenosyl-L-homocysteine
at pH 8.0 and 25°C
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E7BBN5
UniProt
brenda
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UniProt
brenda
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E7BBN5
UniProt
brenda
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UniProt
brenda
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additional information
E7BBN5
cell-free extract, roseoflavin synthesis is observed in the exponentially phase growth, under laboratory conditions using starch and yeast extract containing broth, roseoflavin synthesis is observed in the stationary phase of growth
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brenda
additional information
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cell-free extract, roseoflavin synthesis is observed in the exponentially phase growth, under laboratory conditions using starch and yeast extract containing broth, roseoflavin synthesis is observed in the stationary phase of growth
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brenda
additional information
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cell-free extract, roseoflavin synthesis is observed in the exponentially phase growth, under laboratory conditions using starch and yeast extract containing broth, roseoflavin synthesis is observed in the stationary phase of growth
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brenda
Highest Expressing Human Cell Lines
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Gene Links
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physiological function
E7BBN5
RosA catalyzes the two terminal steps in the biosynthesis of the antibiotic roseoflavin. RosA is the first enzyme of the biosynthesis to be identified
physiological function
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RosA catalyzes the two terminal steps in the biosynthesis of the antibiotic roseoflavin. RosA is the first enzyme of the biosynthesis to be identified
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ROSA_STRDJ
Streptomyces davaonensis (strain DSM 101723 / JCM 4913 / KCC S-0913 / 768)
347
0
37936
Swiss-Prot
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38000
E7BBN5
1 * 38000, calculated, purified recombinant RosA
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homodimer
2 * 38000, SDS-PAGE
homodimer
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2 * 38000, SDS-PAGE
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monomer
E7BBN5
1 * 38000, calculated, purified recombinant RosA
monomer
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1 * 38000, calculated, purified recombinant RosA
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ammonium sulfate precipitation, phenyl-Sepharose chromatography, anion exchange chromatography, gel filtration on a HiLoad 16/60 Superdex 200 column, analyzed using SDS-PAGE and MS/MS
E7BBN5
HisTrap column chromatography and MonoQ column chromatography
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expressed in Escherichia coli Rosetta 2 (DE3) and BL834 (DE3) cells
expressed in Escherichia coli Rosetta 2 (DE3), recombinant His6-tagged RosA purified, ORF sda77220 is named rosA is located in the middle of a putative gene cluster within the genome of Streptomyces davawensis
E7BBN5
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Jankowitsch, F.; Khm, C.; Kellner, R.; Kalinowski, J.; Pelzer, S.; Macheroux, P.; Mack, M.
A novel N,N-8-amino-8-demethyl-D-riboflavin dimethyltransferase (RosA) catalyzing the two terminal steps of roseoflavin biosynthesis in Streptomyces davawensis
J. Biol. Chem.
286
38275-285
2011
Streptomyces davaonensis (E7BBN5), Streptomyces davaonensis, Streptomyces davaonensis 768 (E7BBN5)
brenda
Tongsook, C.; Uhl, M.; Jankowitsch, F.; Mack, M.; Gruber, K.; Macheroux, P.
Structural and kinetic studies on RosA, the enzyme catalysing the methylation of 8-demethyl-8-amino-D-riboflavin to the antibiotic roseoflavin
FEBS J.
283
1531-1549
2016
Streptomyces davaonensis (K4RFM2), Streptomyces davaonensis JCM 4913 (K4RFM2)
brenda
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