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Information on EC - tRNA (cytidine34-2'-O)-methyltransferase

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
       tRNA (cytidine34-2'-O)-methyltransferase
IUBMB Comments
The enzyme from Escherichia coli catalyses the 2'-O-methylation of cytidine or 5-carboxymethylaminomethyluridine at the wobble position at nucleotide 34 in tRNALeuCmAA and tRNALeucmnm5UmAA. The enzyme is selective for the two tRNALeu isoacceptors and only methylates these when they present the correct anticodon loop sequence and modification pattern. Specifically, YibK requires a pyrimidine nucleoside at position 34, it has a clear preference for an adenosine at position 35, and it fails to methylate without prior addition of the N6-(isopentenyl)-2-methylthioadenosine modification at position 37.
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The enzyme appears in viruses and cellular organisms
Reaction Schemes
5-carboxymethylaminomethyluridine34 in tRNALeu
5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNALeu
C/U34 2-O-methyltransferase, methyltransferase yibK, Trml, tRNA (cytidine34/5-carboxymethylaminomethyluridine34'-O)-methyltransferase, tRNA (Um34/Cm34) methyltransferase, tRNA methyltransferase L, YibK, more
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine34 in tRNALeu = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNALeu
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S-adenosyl-L-methionine + cytidine34 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine34 in tRNA
show the reaction diagram
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