Information on EC 2.1.1.157 - sarcosine/dimethylglycine N-methyltransferase

Substrates: (overall reaction)
Products: -

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S-adenosyl-L-methionine + glycine

S-adenosyl-L-homocysteine + sarcosine

Substrates: low activity
Products: -

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S-adenosyl-L-methionine + N,N-dimethylglycine

S-adenosyl-L-homocysteine + betaine

20 entries

S-adenosyl-L-methionine + N,N-dimethylglycine

S-adenosyl-L-homocysteine + betaine + H+

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Substrates: -
Products: -

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S-adenosyl-L-methionine + N,N-dimethylglycine

S-adenosyl-L-homocysteine + N,N,N-trimethylglycine

S-adenosyl-L-methionine + N-methylglycine

S-adenosyl-L-homocysteine + N,N-dimethylglycine

S-adenosyl-L-methionine + sarcosine

S-adenosyl-L-homocysteine + N,N-dimethylglycine

19 entries

additional information

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18 entries

S-adenosyl-L-methionine + N,N-dimethylglycine

S-adenosyl-L-homocysteine + betaine

16 entries

S-adenosyl-L-methionine + N,N-dimethylglycine

S-adenosyl-L-homocysteine + N,N,N-trimethylglycine

S-adenosyl-L-methionine + N-methylglycine

S-adenosyl-L-homocysteine + N,N-dimethylglycine

S-adenosyl-L-methionine + sarcosine

S-adenosyl-L-homocysteine + N,N-dimethylglycine

14 entries

additional information

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7 entries

S-adenosyl-L-methionine

K+

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highest activity at 0.8 M K+

677087

additional information

4-chloromercuribenzoate

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recombinant enzyme: 23% inhibition at 1.33 mM, completely reversible by 5.3 mM DTT

betaine

dimethylglycine

competitive inhibition, versus sarcosine, 40% inhibition at 250 mM

isovaleric acid

10% inhibition at 250 mM, with dimethylglycine

KCl/NaCl

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recombinant enzyme: 32% inhibition at 0.33 M KCl and 0.66 M NaCl with sarcosine, and 25% inhibition at 0.33 M KCl and 0.66 M NaCl with dimethylglycine, decrease inhibition by betaine, overview

L-alanine

10% inhibition at 250 mM, with dimethylglycine

L-cysteine

10% inhibition at 250 mM, with dimethylglycine

L-methionine

10% inhibition at 250 mM, with dimethylglycine

L-phenylalanine

10% inhibition at 250 mM

n-butyric acid

50% inhibition at 250 mM, with dimethylglycine

propionic acid

10% inhibition at 250 mM, with dimethylglycine

S-adenosyl-L-homocysteine

sarcosine

slight competitive inhibition, versus dimethylglycine, 20% inhibition at 250 mM

tert-butylacetic acid

30% inhibition at 250 mM, with dimethylglycine

additional information

-

additional information

abiotic stess-inducible enzyme, e.g. high salt content

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0.5 - 7.5

dimethylglycine

2.8 - 3.76

N,N-dimethylglycine

0.144 - 0.79

S-adenosyl-L-methionine

11 entries

0.8 - 6.1

sarcosine

5 entries

1.4 - 2.68

N,N-dimethylglycine

0.44 - 1.07

sarcosine

0.15 - 0.4

S-adenosyl-L-homocysteine

0.23

activity with sarcosine and S-adenosyl-L-methionine in crude cell extracts

0.34

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activity with sarcosine and S-adenosyl-L-methionine in crude cell extracts

0.36

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activity with sarcosine and S-adenosyl-L-methionine, purified enzyme

0.68

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purified recombinant enzyme, substrate sarcosine

0.71

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pH 7.2, 37°C, sarcosine N-methyltransferase activity

0.93

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activity with N,N-dimethylglycine and S-adenosyl-L-methionine in crude cell extracts

1

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activity with N,N-dimethylglycine and S-adenosyl-L-methionine, purified enzyme

2.6

activity with N,N-dimethylglycine and S-adenosyl-L-methionine in crude cell extracts

3

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pH 7.2, 37°C, N,N-dimethylglycine N-methyltransferase activity

3.4

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purified recombinant enzyme, substrate dimethylglycine

additional information

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7.3

assay at

7.5

7.6

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recombinant enzyme, with substrate dimethylglycine, triethanolamine buffer

8

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methylation of sarcosine

8 - 9

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recombinant enzyme, with substrate sarcosine, pH-optimum depends on the buffer system

8.8

assay at

additional information

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pH-optimum depends on the buffer system

37

6 entries

5

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isoelectric focusing

ATCC 27976

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Aphanothece halophytica 7418

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734643

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Methanohalophilus portucalensis FDF1

gene smdA encodes a Sdm homologue protein

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Thioalkalivibrio versutus D301

Highest Expressing Human Cell Lines

Filter by:

Cell Line Links

Gene Links

malfunction

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disruption of the sdmA gene, encoding Sdm homologue protein, generates a mutant that exhibits a longer lag period of growth and delayed spore germination under osmostress, it shows normal growth in CYE medium at 30°C, phenotype, overview

metabolism

physiological function

7 entries

additional information

25000

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gel filtration

27900

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x * 27900, about, sequence calculation

29000

recombinant enzyme, gel filtration

30690

2 * 30690, sequence calculation, 2 * 38000, SDS-PAGE

31000

1 * 31000, recombinant enzyme, SDS-PAGE

32000

33000

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gel fitlration

34000

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2 * 34000, denaturing PAGE

36000

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1 * 36000, recombinant enzyme, SDS-PAGE, 1 * 32000, about, amino acid sequence calculation

38000

2 * 30690, sequence calculation, 2 * 38000, SDS-PAGE

67700

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gel filtration

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dimer

monomer

hanging drop vapor-diffusion method, crystal structure of the enzyme, solved to a resolution of 1.95 A

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formation of monoclinic crystals, diffracting to 2.15 A resolution, and orthorhombic crystals, diffracting to 1.8 A resolution

695387

M172R

site-directed mutagenesis, reduced activity with sarcosine, but highly reduced activity with dimethylglycine compared to the wild-type enzyme

P171Q

site-directed mutagenesis, reduced activity with sarcosine and dimethylglycine compared to the wild-type enzyme

P171Q/M172R

site-directed mutagenesis, reduced activity with glycine and sarcosine, but highly reduced activity with dimethylglycine compared to the wild-type enzyme

additional information

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construction of a chromosomal sdmA gene disruption knockout mutant, phenotype, overview

61

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melting temperature

-85°C, 5 months, no significant loss of both activities

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by AMP affinity chromatography and ultrafiltration

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recombinant enzyme from Escherichia coli by ammonium sulfate fractionation and ion exchange chromatography to homogeneity

recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, hydrophobic and anion exchange chromatography, and ultrafiltration to homogeneity

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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

A0A0G3G397

777439

recombinant MpSDMT from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

DNA and amino acid sequence determination, the single gene encodes the sarcosine dimethylglycine methyltransferase as well as the glycine sarcosine methyltransferase, EC 2.1.1.156, the first being probably a proteolytic processing product of the latter, functional expression of a truncated soluble enzyme form in Escherichia coli

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expressed in Anabaena doliolum

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734643

expressed in Anabaena sp. PCC7120

760449

expressed in Escherichia coli BL21(DE3) cells

A0A0G3G397

777439

expressed in Oryza sativa cultivar Nipponbare

733736

expressed in Zea mays

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734671

expression in Escherichia coli

functional overexpression in Escherichia coli strain XL-1 Blue conferring betaine accumulation and increased salt tolerance when coexpressed with the glycine sarcosine methyltransferase, EC 2.1.1.156

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gene Mpsdmt, DNA and amino acid sequence determination and analysis and sequence comparisons, phylogenetic analysis of SDMT, expression of complete gene cluster of Mpgsmt-sdmt in Escherichia coli strain BL21(DE3) and in strain MKH13 deficient in betaine transport, the recombinant enzyme is functional to synthesize and accumulate betaine and confers elevated survival ability in betaine transport deficient mutant strain MKH13 under high salt stress

gene sdmA, DNA and amino acid sequence determination and analysis, sequence comparison

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orf2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of wild-type and mutant enzymes in Escherichia coli, functional coexpression of the soluble enzyme with the glycine sarcosine methyltransferase, EC 2.1.1.156, increases the intracellular betaine level and the growth rate of Escherichia coli strain BL21(DE3)

recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)

A0A0G3G397

777439

betaine downregulates GSMT expression enhanced with increasing temperature, transcription unit and transcription level of Mpgsmt-sdmt is reduced under temperature stresses up to 37�C, but recovers at 45�C to the level at 20�C

the enzyme shows increased expression levels under drought conditions

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734671

agriculture

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enzyme can be used in betaine production for improvement of stress tolerance of commercially important microbes in agriculture and industry, and for nutritial improvement of transgenic crop plants, that do not produce betaine naturally

biotechnology

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enzyme can be used in betaine production for improvement of stress tolerance of commercially important microbes in agriculture and industry, and for nutritial improvement of transgenic crop plants, that do not produce betaine naturally

nutrition

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enzyme can be used in betaine production for improvement of stress tolerance of commercially important microbes in agriculture and industry, and for nutritial improvement of transgenic crop plants, that do not produce betaine naturally