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Information on EC 2.1.1.140 - (S)-coclaurine-N-methyltransferase
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EC Tree
2.1.1.140 (S)-coclaurine-N-methyltransferaseIUBMB Comments
The enzyme is specific for the (S)-isomer of coclaurine. Norcoclaurine can also act as an acceptor.
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Word Map
- 2.1.1.140
-
benzylisoquinoline
-
n-methylated
- japonica
- coptis
- isoquinoline
- norreticuline
-
s-reticuline
- n-methyltransferases
- norcoclaurine
- poppy
- scoulerine
- somniferum
-
3\'-hydroxylase
-
4\'omt
- magnoflorine
-
6-o-methyltransferase
-
opium
-
papaver
- tetrahydroisoquinoline
-
aporphine
- medicine
- biotechnology
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
coclaurine n-methyltransferase, s-adenosyl-l-methionine:coclaurine n-methyltransferase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(S)-Coclaurine N-methyltransferase
CNMT
coclaurine N-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + (S)-coclaurine = S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
transfer of methyl group
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:(S)-coclaurine-N-methyltransferase
The enzyme is specific for the (S)-isomer of coclaurine. Norcoclaurine can also act as an acceptor.
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CAS REGISTRY NUMBER
COMMENTARY
167398-06-3
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(R)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (R)-N-methylcoclaurine
-
-
-
?
(R,S)-6-O-methylnorlaudanosoline + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (R,S)-N-methyl-6-O-methyl-norlaudanosoline
-
-
-
?
(R,S)-norlaudanosoline + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (R,S)-N-methylnorlaudanosoline
-
-
-
?
(R,S)-norreticuline + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (R,S)-N-methylnorreticuline
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
(S)-norcoclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (R)-N-methylnorcoclaurine
-
strictly stereospecific, only (S)-norcoclaurine is N-methylated
-
?
1-methyl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline + S-adenosyl-L-methionine
N-methyl-1-methyl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline + S-adenosyl-L-methionine
-
-
-
?
6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline + S-adenosyl-L-methionine
N-methyl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline + S-adenosyl-L-methionine
-
-
-
?
S-adenosyl-L-methionine + (R)-coclaurine
S-adenosyl-L-homocysteine + (R)-N-methylcoclaurine
S-adenosyl-L-methionine + (R)-N-methylcoclaurine
S-adenosyl-L-homocysteine + (R)-magnocurarine
-
-
-
?
S-adenosyl-L-methionine + (R,S)-6-O-methylnorlaudanosoline
S-adenosyl-L-homocysteine + (R,S)-6-O-methyllaudanosoline
-
-
-
?
S-adenosyl-L-methionine + (R,S)-6-O-methylnorlaudanosoline
S-adenosyl-L-homocysteine + (R,S)-N-methyl-6-O-methylnorlaudanosoline
S-adenosyl-L-methionine + (R,S)-norlaudanosoline
S-adenosyl-L-homocysteine + (R,S)-N-methylnorlaudanosoline
S-adenosyl-L-methionine + (R,S)-norreticuline
S-adenosyl-L-homocysteine + (R,S)-N-methylnorreticuline
-
55% of the activity with (S)-coclaurine
-
-
?
S-adenosyl-L-methionine + (RS)-coclaurine
S-adenosyl-L-homocysteine + (RS)-N-methylcoclaurine
-
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
S-adenosyl-L-methionine + (S)-N-methylcoclaurine
S-adenosyl-L-homocysteine + ?
weak activity
-
-
?
S-adenosyl-L-methionine + (S)-reticuline
S-adenosyl-L-homocysteine + (S)-tembetarine
-
-
-
?
S-adenosyl-L-methionine + 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline
S-adenosyl-L-homocysteine + 6,7-dihydroxy-1,N-dimethyl-1,2,3,4-tetrahydroisoquinoline
S-adenosyl-L-methionine + 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline
S-adenosyl-L-homocysteine + 6,7-dimethoxy-N-methyl-1,2,3,4-tetrahydroisoquinoline
-
39% of the activity with (S)-coclaurine
-
-
?
S-adenosyl-L-methionine + 6,7-dimethoxy-N-methyl-1,2,3,4-tetrahydroisoquinoline
S-adenosyl-L-homocysteine + 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline
-
180% of the activity with (R,S)-norreticuline
-
-
?
S-adenosyl-L-methionine + heliamine
S-adenosyl-L-homocysteine + N-methylheliamine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
Berberis koetineana
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
Bocconia cordata
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
Corydalis vaginans
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
Thalictrum glaucum
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
strictly stereospecific, only (S)-coclaurine is N-methylated
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
Tinospora fragosa
-
-
-
?
S-adenosyl-L-methionine + (R)-coclaurine
S-adenosyl-L-homocysteine + (R)-N-methylcoclaurine
-
122% of the activity with (S)-coclaurine
-
-
?
S-adenosyl-L-methionine + (R)-coclaurine
S-adenosyl-L-homocysteine + (R)-N-methylcoclaurine
-
240% of the activity with (R,S)-norreticuline
-
-
?
S-adenosyl-L-methionine + (R)-coclaurine
S-adenosyl-L-homocysteine + (R)-N-methylcoclaurine
-
-
-
?
S-adenosyl-L-methionine + (R,S)-6-O-methylnorlaudanosoline
S-adenosyl-L-homocysteine + (R,S)-N-methyl-6-O-methylnorlaudanosoline
-
38% of the activity with (S)-coclaurine
-
-
?
S-adenosyl-L-methionine + (R,S)-6-O-methylnorlaudanosoline
S-adenosyl-L-homocysteine + (R,S)-N-methyl-6-O-methylnorlaudanosoline
-
69% of the activity with (R,S)-norreticuline
-
-
?
S-adenosyl-L-methionine + (R,S)-norlaudanosoline
S-adenosyl-L-homocysteine + (R,S)-N-methylnorlaudanosoline
-
48% of the activity with (S)-coclaurine
-
-
?
S-adenosyl-L-methionine + (R,S)-norlaudanosoline
S-adenosyl-L-homocysteine + (R,S)-N-methylnorlaudanosoline
-
49% of the activity with (R,S)-norreticuline
-
-
?
S-Adenosyl-L-methionine + (S)-coclaurine
?
-
pathway for the biosynthesis of the benzyltetrahydroisoquinoline alkaloid reticuline: (S)-norcoclaurine is stereoselectively metabolized to (S)-reticuline via (S)-coclaurine, (S)-N-methylcoclaurine and (S)-3'-hydroxy-N-methylcoclaurine
-
-
?
S-Adenosyl-L-methionine + (S)-coclaurine
?
-
pathway for the biosynthesis of the benzyltetrahydroisoquinoline alkaloid reticuline: (S)-norcoclaurine is stereoselectively metabolized to (S)-reticuline via (S)-coclaurine, (S)-N-methylcoclaurine and (S)-3'-hydroxy-N-methylcoclaurine
-
-
?
S-Adenosyl-L-methionine + (S)-coclaurine
?
-
pathway for the biosynthesis of the benzyltetrahydroisoquinoline alkaloid reticuline: (S)-norcoclaurine is stereoselectively metabolized to (S)-reticuline via (S)-coclaurine, (S)-N-methylcoclaurine and (S)-3'-hydroxy-N-methylcoclaurine
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
isoquinoline alkaloid biosynthesis
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
sequential bi-substrate mechanism, ordered bi bi or rapid equilibrium random bi bi
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
sequential mechanism, 153% of the activity with (R,S)-norreticuline
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
isoquinoline alkaloid biosynthesis
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
expression systems tested by analyzing recombinant CMT protein
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
analysis of the evolutionary recruitment of enzymes into plant alkaloid pathways
-
-
?
S-Adenosyl-L-methionine + (S)-coclaurine
S-Adenosyl-L-homocysteine + N-methylcoclaurine
-
-
-
?
S-Adenosyl-L-methionine + (S)-coclaurine
S-Adenosyl-L-homocysteine + N-methylcoclaurine
-
-
-
?
S-Adenosyl-L-methionine + (S)-coclaurine
S-Adenosyl-L-homocysteine + N-methylcoclaurine
-
-
-
?
S-adenosyl-L-methionine + 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline
S-adenosyl-L-homocysteine + 6,7-dihydroxy-1,N-dimethyl-1,2,3,4-tetrahydroisoquinoline
-
10% of the activity with (S)-coclaurine
-
-
?
S-adenosyl-L-methionine + 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline
S-adenosyl-L-homocysteine + 6,7-dihydroxy-1,N-dimethyl-1,2,3,4-tetrahydroisoquinoline
-
15% of the activity with (R,S)-norreticuline
-
-
?
additional information
?
-
-
no activity with (R,S)-scoulerine, 1,2,3,4-tetrahydroisoquinoline, 1,2,3,4-tetrahydro-3-isoquinolinecarboxylic acid or (+)-emetine
-
-
?
additional information
?
-
-
no activity with: (R,S)-scoulerine, 1,2,3,4-tetrahydroisoquinoline and 1,2,3,4-tetrahydro-3-isoquinoline carboxylic acid (+)-emetine
-
-
?
additional information
?
-
-
involvement in magnoflorine biosynthesis determined, crude enzyme preparation of recombinant CNMT protein analyzed, recombinant CjCNMT protein shown to convert (S)-corytuberine to magnoflorine
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
Berberis koetineana
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
Bocconia cordata
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
Corydalis vaginans
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
Thalictrum glaucum
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
(S)-coclaurine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
Tinospora fragosa
-
-
-
?
S-Adenosyl-L-methionine + (S)-coclaurine
?
-
pathway for the biosynthesis of the benzyltetrahydroisoquinoline alkaloid reticuline: (S)-norcoclaurine is stereoselectively metabolized to (S)-reticuline via (S)-coclaurine, (S)-N-methylcoclaurine and (S)-3'-hydroxy-N-methylcoclaurine
-
-
?
S-Adenosyl-L-methionine + (S)-coclaurine
?
-
pathway for the biosynthesis of the benzyltetrahydroisoquinoline alkaloid reticuline: (S)-norcoclaurine is stereoselectively metabolized to (S)-reticuline via (S)-coclaurine, (S)-N-methylcoclaurine and (S)-3'-hydroxy-N-methylcoclaurine
-
-
?
S-Adenosyl-L-methionine + (S)-coclaurine
?
-
pathway for the biosynthesis of the benzyltetrahydroisoquinoline alkaloid reticuline: (S)-norcoclaurine is stereoselectively metabolized to (S)-reticuline via (S)-coclaurine, (S)-N-methylcoclaurine and (S)-3'-hydroxy-N-methylcoclaurine
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
isoquinoline alkaloid biosynthesis
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
isoquinoline alkaloid biosynthesis
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
-
?
S-adenosyl-L-methionine + (S)-coclaurine
S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
S-adenosyl-L-methionine
-
50% inhibition at 0.062 mM, substrate (S)-coclaurine
additional information
-
no inhibition by PCMB, iodoacetamide; not inhibited by EDTA, iodoacetamide, p-chloromercuribenzoate
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.05
(S)-norcoclaurine
-
probably allosteric enzyme, Hill coeffizient 2.6
0.265
(RS)-coclaurine
wild-type, pH not specified in the publication, temperature not specified in the publication
0.601
(RS)-coclaurine
mutant Y328A, pH not specified in the publication, temperature not specified in the publication
1.033
(RS)-coclaurine
mutant W329A, pH not specified in the publication, temperature not specified in the publication
1.726
(RS)-coclaurine
mutant F332A, pH not specified in the publication, temperature not specified in the publication
0.311
heliamine
wild-type, pH not specified in the publication, temperature not specified in the publication
1.565
heliamine
mutant W329A, pH not specified in the publication, temperature not specified in the publication
2.096
heliamine
mutant Y328A, pH not specified in the publication, temperature not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.01
(RS)-coclaurine
mutant W329A, pH not specified in the publication, temperature not specified in the publication
0.02
(RS)-coclaurine
mutant F332A, pH not specified in the publication, temperature not specified in the publication
0.12
(RS)-coclaurine
mutant Y328A, pH not specified in the publication, temperature not specified in the publication
0.53
(RS)-coclaurine
wild-type, pH not specified in the publication, temperature not specified in the publication
0.14
heliamine
mutant W329A, pH not specified in the publication, temperature not specified in the publication
0.2
heliamine
mutant Y328A, pH not specified in the publication, temperature not specified in the publication
0.6
heliamine
wild-type, pH not specified in the publication, temperature not specified in the publication
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.01
(RS)-coclaurine
mutant F332A, pH not specified in the publication, temperature not specified in the publication
0.01
(RS)-coclaurine
mutant W329A, pH not specified in the publication, temperature not specified in the publication
0.2
(RS)-coclaurine
mutant Y328A, pH not specified in the publication, temperature not specified in the publication
2
(RS)-coclaurine
wild-type, pH not specified in the publication, temperature not specified in the publication
0.09
heliamine
mutant W329A, pH not specified in the publication, temperature not specified in the publication
0.09
heliamine
mutant Y328A, pH not specified in the publication, temperature not specified in the publication
2
heliamine
wild-type, pH not specified in the publication, temperature not specified in the publication
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.000096
0.0001
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.0003
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.00065
Thalictrum glaucum
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.00066
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.0007
Corydalis vaginans
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.00075
Bocconia cordata
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.00077
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.0013
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.002
0.003
Tinospora fragosa
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
additional information
0.000096
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.000096
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
additional information
-
(S)-corytuberine conversion to magnoflorine shown by crude enzyme preparation of recombinant CNMT protein, magnoflorine biosynthesis by CYP80G2 protein and CjCNMT via (S)-corytuberine as an intermediate in vivo, CjCNMT protein catalyzes the crucial N-methylation step from (S)-coclaurine to (S)-N-methylcoclaurine in (S)-reticuline biosynthesis
additional information
-
analysis of inducible expression systems, production of recombinant CMT protein from PprpB promoter 2fold to 4fold higher than that from PT7 promoter, cell growth not reduced after expression form PprpB promoter even when highly induced, propionate-inducible system efficient for protein overproduction that result in growth inhibition, auto-induction experiments performed and protein production monitored
additional information
phylogenetic analysis reveales a monophyletic clade for (S)-coclaurine N-methyltransferase (CNMT) and (S)-tetrahydroprotoberberine cis-N-methyltransferase (TNMT), 48% identity of amino acid sequence, downstream metabolic role suggests that TNMT derives from gene duplication after more ancient recruitment of CNMT, phylogenetic relationship between CNMT and TNMT provides new insights into evolutionary recruitment of enzymes into plant alkaloid pathways
additional information
-
phylogenetic analysis reveales a monophyletic clade for (S)-coclaurine N-methyltransferase (CNMT) and (S)-tetrahydroprotoberberine cis-N-methyltransferase (TNMT), 48% identity of amino acid sequence, downstream metabolic role suggests that TNMT derives from gene duplication after more ancient recruitment of CNMT, phylogenetic relationship between CNMT and TNMT provides new insights into evolutionary recruitment of enzymes into plant alkaloid pathways
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
