Information on EC 2.1.1.139 - 3'-demethylstaurosporine O-methyltransferase

for references in articles please use BRENDA:EC2.1.1.139
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.1.1.139
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RECOMMENDED NAME
GeneOntology No.
3'-demethylstaurosporine O-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 3'-demethylstaurosporine = S-adenosyl-L-homocysteine + staurosporine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer of methyl group
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
staurosporine biosynthesis
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Staurosporine biosynthesis
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:3'-demethylstaurosporine O-methyltransferase
Catalyses the final step in the biosynthesis of staurosporine, an alkaloidal antibiotic that is a potent inhibitor of protein kinases, especially protein kinase C.
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CAS REGISTRY NUMBER
COMMENTARY hide
212906-74-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3'-demethylstaurosporine
S-adenosyl-L-homocysteine + staurosporine
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3'-demethylstaurosporine
S-adenosyl-L-homocysteine + staurosporine
show the reaction diagram
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1H-indole
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62% activity left at 1.1 mM
3'-demethylstaurosporine
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69% activity at 1.1 mM
anthranilate
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75% activity left at 1.1 mM
Indole-3-pyruvate
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44% activity left at 1.1 mM
S-(5'-adenosyl)-L-homocysteine
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strong inhibition, 8% activity left at 1.1 mM, probably competitive inhibition
sinefungin
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antiprotozoal antibiotic from Streptomyces griseolus, efficient inhibition with concentrations higher than 1 mg/100 ml
tryptophan
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64% activity left at 1.1 mM
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
tryptophan
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2fold increase at 0.2 mM
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
anion exchange chromatography, partial purification
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LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.139)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)