Information on EC 1.97.1.9 - selenate reductase (cytochrome c)

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Substrates: -
Products: -

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selenate + electron donor

selenite + H2O + oxidized donor

selenate + electron donor

selenite + H2O + oxidized electron donor

14 entries

selenate + glucose

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Substrates: -
Products: -

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selenate + H2

selenite + H2O

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658902

Substrates: -
Products: -

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selenate + lactate

selenite + H2O + acetate + HCO3-

Anaerobacillus arseniciselenatis

selenate + malate

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Substrates: -
Products: -

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selenate + NADH + H+

selenite + H2O + NAD+

selenate + pyruvate

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selenate + reduced acceptor

selenite + H2O + acceptor

24 entries

selenate + reduced benzyl viologen

selenite + H2O + benzyl viologen

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Substrates: -
Products: -

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selenate + reduced benzyl viologen

selenite + H2O + oxidized benzyl viologen

selenate + reduced cytc-Ts4

selenite + H2O + oxidized cytc-Ts4

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715508

Substrates: a c-type cytochrom is purified and shown to donate electrons to SerABC in vitro. Redox potentiometry, combined with UV-visible spectroscopy, show that cytc-Ts4 is a diheme cytochrome with a redox potential of +/-282 mV, and both hemes are predicted to have His-Met ligation
Products: -

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selenate + reduced cytochrome c4

selenite + H2O + cytochrome c4

Substrates: -
Products: -

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selenate + reduced methyl viologen

selenite + H2O + methyl viologen

selenate + reduced methyl viologen

selenite + H2O + oxidized methyl viologen

7 entries

selenite + 3 NADH + 3 H+

selenium + 3 H2O + 3 NAD+

tellurate + reduced acceptor

tellurite + H2O + acceptor

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767066

Substrates: -
Products: -

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additional information

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17 entries

selenate + electron donor

selenite + H2O + oxidized electron donor

5 entries

selenate + H2

selenite + H2O

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658902

Substrates: -
Products: -

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selenate + NADH + H+

selenite + H2O + NAD+

selenate + reduced acceptor

selenite + H2O + acceptor

23 entries

selenate + reduced cytochrome c4

selenite + H2O + cytochrome c4

Substrates: -
Products: -

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selenite + 3 NADH + 3 H+

selenium + 3 H2O + 3 NAD+

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Substrates: -
Products: -

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tellurate + reduced acceptor

tellurite + H2O + acceptor

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767066

Substrates: -
Products: -

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additional information

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10 entries

cytochrome b

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heme

heme b

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iron-sulfur center

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activity of genes iscU or hscB in the Isc iron-sulfur cluster biosynthesis pathway is required for formation of selenate reductase activity in vivo

744931

iron-sulfur centre

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at least 2 [Fe-S]-centre as prosthetic groups per enzyme molecule

molybdenum cofactor

molybdopterin

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[Fe-S]-center

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Iron

6 entries

Mo2+

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role of Mo2+ in selenate reductase

Molybdenum

non-heme iron

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selenium

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isolated enzyme contains a reduced form of selenium, probably as selenocysteine

658902

sulfur

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iron-sulfur centre

658902, 657903

additional information

Ag+

Ca2+

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about 20% inhibition at 1 mM

Cu2+

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EDTA

Hg2+

methanol

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the activity of reductase decreases in the presence of 10 and 20% (v/v) methanol to about 40 and 30% of the original activity, respectively

Mn2+

N-ethyl maleimide

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complete inhibition

N-ethylmaleimide

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complete inhibition at 1 mM

NaN3

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60% inhibition at 1 mM

Pb2+

Propanol

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the activity of reductase decreases in the presence of 5-20% (v/v) of propanol to about 20% of the original activity

selenate

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mixed-type inhibition

671304

Sodium azide

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about 35% residual activity at 1 mM

thiocyanate

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mixed-type inhibition

671304

tungstate

Zn2+

[BMIm][Br]

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the activity of the enzyme decreases in the presence of the ionic liquid [BMIm][Br] (about 35% residual activity at 20% (v/v))

[BMIm][Cl]

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the activity of the enzyme decreases in the presence of the ionic liquid [BMIm][Cl] (about 25% residual activity at 20% (v/v))

additional information

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molybdate

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selenate

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induction of enzyme activity when included in growth medium

6.25

benzyl viologen

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20°C

0.0007 - 6.25

selenate

11 entries

5.47

Selenite

additional information

8 entries

-

5 - 387

selenate

2.9

thiocyanate

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30°C

671304

0.29

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membrane fraction

0.39

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cell extract, at pH 6.0 and 35°C

0.42

wild-type

644813

0.76

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wild-type, cells grown on nitrate

1.6

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wild-type, cells grown on selenate + nitrate

15.36

3.84

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wild-type, cells grown on selenate

41.4

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purified enzyme

644811

500

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pH 7.2, 30°C

additional information

6

7.5

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assay at

8

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5 - 9

20

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assay at

35

37

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in vivo assay at

744931

65

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optimal temperature for molybdenum-SER

714365

80

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optimal temperature for tungsten-SER is higher than 80°C

714365

20 - 70

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activity range, profile overview, 70% and 35% of maximum activity at 50°C and 60°C

35 - 55

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more than 60% activity between 35 and 55°C. The enzyme reveals 70% and 35% of maximum activity at 50°C and 60°C, respectively

Anaerobacillus arseniciselenatis

additional information

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maximal growth at a pH range of pH 9.0-11.0

isolated from Sabzevar of Iran

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Anaerobacillus arseniciselenatis

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Clostridium sp.

isolated from sediment layer of seven locations on two mine sites, one near the town of Likely in British Columbia, Canada and the other in the Elk Valley in British Columbia, Canada

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Enterobacter cloacae SLD1a-1 / ATCC 700258

Enterobacter cloacae SLDa-1

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Escherichia coli K-12

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697647

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Exiguobacterium sp.

isolated from sediment layer of seven locations on two mine sites, one near the town of Likely in British Columbia, Canada and the other in the Elk Valley in British Columbia, Canada

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Gottschalkia acidurici

isolated from sediment layer of seven locations on two mine sites, one near the town of Likely in British Columbia, Canada and the other in the Elk Valley in British Columbia, Canada

K0B418

UniProt

Gottschalkia acidurici ATCC 7906

isolated from sediment layer of seven locations on two mine sites, one near the town of Likely in British Columbia, Canada and the other in the Elk Valley in British Columbia, Canada

K0B418

UniProt

Lysinibacillus sp.

isolated from sediment layer of seven locations on two mine sites, one near the town of Likely in British Columbia, Canada and the other in the Elk Valley in British Columbia, Canada

Mesobacillus selenatarsenatis

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Mesobacillus selenatarsenatis SF-1

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Salmonella enterica subsp. enterica serovar Typhimurium

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Salmonella enterica subsp. enterica serovar Typhimurium LT2a

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Schnuerera ultunensis

isolated from sediment layer of seven locations on two mine sites, one near the town of Likely in British Columbia, Canada and the other in the Elk Valley in British Columbia, Canada

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Stutzerimonas stutzeri

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Sulfurospirillum barnesii

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644812, 657903

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Sulfurospirillum barnesii SES-3

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Thauera aminoaromatica

isolated from sediment layer of seven locations on two mine sites, one near the town of Likely in British Columbia, Canada and the other in the Elk Valley in British Columbia, Canada

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Thauera selenatis ATCC 55363

5 entries

alpha, beta, and gamma subunits serA, serB, and serC of selenate reductase; from selenate-reducing membrane biofilm reactor (MBfR) biofilms

UniProt

Thauera sp.

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Tissierella sp.

isolated from sediment layer of seven locations on two mine sites, one near the town of Likely in British Columbia, Canada and the other in the Elk Valley in British Columbia, Canada

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additional information

Highest Expressing Human Cell Lines

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Cell Line Links

Gene Links

metabolism

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activity of genes iscU or hscB in the Isc iron-sulfur cluster biosynthesis pathway is required for formation of selenate reductase activity in vivo. Mutant strains JW2513 and JW2511 devoid of either the iscU or hscB gene in the Isc ironsulfur cluster biosynthesis pathway lose the ability to reduce selenate, phenotypes, overview. Genetic complementation by the wildtype sequences restored selenate reductase activity. The Isc biosynthetic system plays a key role in selenate reductase Fe-S cofactor assembly and is essential for enzyme activity

744931

physiological function

Sulfurospirillum barnesii

additional information

160000

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180000

197000

21000

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alphabetagammadelta, 1 * 82000 + 1 * 53000 + 1 * 34000 + 1 * 21000

23000

250000

GST-tagged enzyme, gel filtration

Sulfurospirillum barnesii

34000

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alphabetagammadelta, 1 * 82000 + 1 * 53000 + 1 * 34000 + 1 * 21000

36000

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3* 100, alpha-subunit, 3 * 55000, beta-subunit, 3 * 36000, gamma-subunit, SDS-PAGE

37000

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1 * 99000, alpha, + 1 * 37000, beta, + 1 * 23000, gamma, SDS-PAGE

40000

53000

55000

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3* 100, alpha-subunit, 3 * 55000, beta-subunit, 3 * 36000, gamma-subunit, SDS-PAGE

600000

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gel filtration

657903, 671468

700000

Pseudomonas sp.

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native gel electrophoresis of cell-free extracts and activity stain

82000

96000

99000

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1 * 99000, alpha, + 1 * 37000, beta, + 1 * 23000, gamma, SDS-PAGE

heterotetramer

heterotrimer

nonamer

tetramer

trimer

additional information

7 entries

hanging-drop vapour diffusion method, precipitation by ammonium sulfate, protein solution: 10 mg/ml, 0.3-0.5 M ammonium sulfate, 50 mM piperazine, pH 6.0, reservoir solution: 1.8-2.2 M ammonium sulfate, 100 mM Tris-HCl, pH 8.0-8.9, 293 K, 2-4 weeks, cryoprotection by 25% glycerol, X-ray structure determination and analysis

Salmonella enterica subsp. enterica serovar Typhimurium

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644814

A28Q

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site-directed mutagenesis, the mutant is devoid of selenate reductase activity and can be rescued by providing chaperon protein DmsD in excess

Salmonella enterica subsp. enterica serovar Typhimurium

F35Q

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site-directed mutagenesis

Salmonella enterica subsp. enterica serovar Typhimurium

L24Q

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site-directed mutagenesis, the mutant is devoid of selenate reductase activity

Salmonella enterica subsp. enterica serovar Typhimurium

L33Q

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site-directed mutagenesis, the mutant is still able to correctly assemble selenate reductase

Salmonella enterica subsp. enterica serovar Typhimurium

V31Q

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site-directed mutagenesis

Salmonella enterica subsp. enterica serovar Typhimurium LT2a

A28Q

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site-directed mutagenesis, the mutant is devoid of selenate reductase activity and can be rescued by providing chaperon protein DmsD in excess

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F35Q

Salmonella enterica subsp. enterica serovar Typhimurium LT2a

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site-directed mutagenesis

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L24Q

Salmonella enterica subsp. enterica serovar Typhimurium LT2a

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site-directed mutagenesis, the mutant is devoid of selenate reductase activity

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L33Q

Salmonella enterica subsp. enterica serovar Typhimurium LT2a

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site-directed mutagenesis, the mutant is still able to correctly assemble selenate reductase

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V31Q

Salmonella enterica subsp. enterica serovar Typhimurium LT2a

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site-directed mutagenesis

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additional information

8 entries

60

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purified molybdenum-SER complex is stable and active upon heat-shock incubation for 10 min at temperatures up to 60°C. At temperatures greater than 65°C all three subunits (SerABC) are readily denatured

714365

60.1

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melting temperature

additional information

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thermal stability analysis by differential scanning calorim

Methanol

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decreases enzyme activity but increases thermal inactivation of the enzyme

n-propanol

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decreases enzyme activity but increases thermal inactivation of the enzyme

-20°C, enzyme extracted with Thesit retains 50% activity after being frozen for prolonged periods

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4°C, enzyme extracted with Thesit remains active for 24 h

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57fold, to near homogeneity

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644811

ammonium sulfate precipitation, Q-Sepharose column chromatography, phenyl Sepharose column chromatography, and G-100 gel filtration

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native enzyme 21.29fold by ammonium sulfate fractionation, anion exchange and hydrophonic interaction chromatography, followed by ultrafiltration

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DNA sequence determination and analysis, contruction of gene bank, genomic organisationand potential function of: genes serA, serB, and serC, additional overlapping serD

644813

expressed in Escherichia coli BL21(DE3) cells

Mesobacillus selenatarsenatis

expression in Escherichia coli

E9RFD1, E9RFC9, E9RFD0

727752

gene serA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative PCR expression analysis

gene serA, quantitative PCR expression analysis

Salmonella enterica subsp. enterica serovar Typhimurium

gene ynfE, encoded by the ynfEFGH-dmsD operon, the YnfE protein is synthesized with an N-terminal twin-arginine signal peptide and biosynthesis of the enzyme is coordinated by a signal peptide binding chaperone called DmsD

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