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Information on EC 1.8.4.8 - phosphoadenylyl-sulfate reductase (thioredoxin) and Organism(s) Pseudomonas aeruginosa

for references in articles please use BRENDA:EC1.8.4.8

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IUBMB Comments

Specific for PAPS. The enzyme from Escherichia coli will use thioredoxins from other species.

The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

Synonyms
aps reductase, paps reductase, adenosine 5'-phosphosulfate reductase, paps sulfotransferase, 3'-phosphoadenosine 5'-phosphosulfate reductase, adenosine 3'-phosphate 5'-phosphosulfate reductase, 5'-adenylylsulfate reductase, adenylyl phosphosulfate reductase, 3'-phosphoadenylylsulfate reductase, cysh1, more

SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3'-Phosphoadenosine 5'-phosphosulfate reductase
-
-
-
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3'-Phosphoadenylylsulfate reductase
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-
-
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5'-adenylylsulfate reductase
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Adenosine 3'-phosphate 5'-phosphosulfate reductase
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-
-
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Adenylyl phosphosulfate reductase
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-
-
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APS reductase
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PAdoPS reductase
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-
-
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PAPS reductase
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-
-
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PAPS reductase, thioredoxin dependent
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-
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PAPS reductase, thioredoxin-dependent
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-
-
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PAPS sulfotransferase
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-
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Phospho-adenylylsulfate (PAPS) reductase
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Phospho-adenylylsulfate reductase
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Reductase, 3'-phosphoadenosine 5'-phosphosulfate
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Thioredoxin: 3'-phospho-adenylylsulfate reductase
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Thioredoxin:adenosine 3'-phosphate 5'-phosphosulfate reductase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
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-
-
-
PATHWAY SOURCE
PATHWAYS
MetaCyc
assimilatory sulfate reduction I, assimilatory sulfate reduction IV
SYSTEMATIC NAME
IUBMB Comments
adenosine 3',5'-bisphosphate,sulfite:thioredoxin-disulfide oxidoreductase (3'-phosphoadenosine-5'-phosphosulfate-forming)
Specific for PAPS. The enzyme from Escherichia coli will use thioredoxins from other species.
CAS REGISTRY NUMBER
COMMENTARY hide
9068-63-7
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY hide
Reversibility
r=reversible
ir=irreversible
?=not specified
adenosine 5'-phosphosulfate + thioredoxin I
AMP + sulfite + oxidized thioredoxin I
show the reaction diagram
-
Substrates: -
Products: -
?
additional information
?
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Substrates: thioredoxin I mutant W31A shows no detectable activity, whereas W31F, K36E, and D61N are able to serve as electron donors for the APR-catalyzed reaction but with lower turnover numbers than that exhibited by the wild type thioredoxin I. The Km for thioredoxin mutant R73E is increased by 7.7fold compared with wild type thioredoxin I
Products: -
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thioredoxin
-
dependent on
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
thioredoxin I
-
wild type enzyme, at 20°C in 10 mM phosphate buffer (pH 7.5) containing 100 mM Na2SO4
top print hide Go to Organism Search
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Highest Expressing Human Cell Lines
Cell Line Links Gene Links
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chung, J.S.; Noguera-Mazon, V.; Lancelin, J.M.; Kim, S.K.; Hirasawa, M.; Hologne, M.; Leustek, T.; Knaff, D.B.
Interaction domain on thioredoxin for Pseudomonas aeruginosa 5'-adenylylsulfate reductase
J. Biol. Chem.
284
31181-31189
2009
Pseudomonas aeruginosa
Manually annotated by BRENDA team