Information on EC 1.5.99.14 - 6-hydroxypseudooxynicotine dehydrogenase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
1.5.99.14
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RECOMMENDED NAME
GeneOntology No.
6-hydroxypseudooxynicotine dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + acceptor + H2O = 1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one + reduced acceptor
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
nicotine degradation I (pyridine pathway)
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Nicotinate and nicotinamide metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one:acceptor 6-oxidoreductase (hydroxylating)
Contains a cytidylyl molybdenum cofactor [3]. The enzyme, which participates in the nicotine degradation pathway, has been characterized from the soil bacterium Arthrobacter nicotinovorans [1,2].
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
encoded on megaplasmid pAO1
UniProt
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + acceptor + H2O
1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one + reduced acceptor
show the reaction diagram
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6-hydroxypseudooxynicotine + 2,6-dichlorophenolindophenol + H2O
6-hydroxy-3-succinoylsemialdehyde-pyridine + methylamine + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
contains 1 mol FAD, 1 mol molybdenum, 4 mol iron and 2 mol labile sulfur per mol of enzyme
molybdopterin cytosine dinucleotide
cofactor is required for assembly of the heterotrimeric molybdenum enzyme
[4Fe-4S]-center
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
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contains 1 mol FAD, 1 mol molybdenum, 4 mol iron and 2 mol labile sulfur per mol of enzyme
Molybdenum
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contains 1 mol FAD, 1 mol molybdenum, 4 mol iron and 2 mol labile sulfur per mol of enzyme
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28.3
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37�C, pH 7.5
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15000
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1 * 82000, 1 * 30000, 1 * 15000, SDS-PAGE
30000
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1 * 82000, 1 * 30000, 1 * 15000, SDS-PAGE
82000
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1 * 82000, 1 * 30000, 1 * 15000, SDS-PAGE
120000
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gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterotrimer
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1 * 82000, 1 * 30000, 1 * 15000, SDS-PAGE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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Show AA Sequence (158 entries)
Please use the Sequence Search for a specific query.
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LINKS TO OTHER DATABASES (specific for EC-Number 1.5.99.14)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)