We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments A CuII enzyme found in higher plants that produces oxidized forms of the benzophenanthridine alkaloids
The enzyme appears in viruses and cellular organisms
Synonyms
DBO, DBOX, DHBP, Oxidase, dihydrobenzophenanthridine,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Oxidase, dihydrobenzophenanthridine
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dihydrochelirubine + O2 = chelirubine + H2O2
-
-
-
-
dihydromacarpine + O2 = macarpine + H2O2
-
-
-
-
dihydrosanguinarine + O2 = sanguinarine + H2O2
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dihydrobenzophenanthridine:oxygen oxidoreductase
A CuII enzyme found in higher plants that produces oxidized forms of the benzophenanthridine alkaloids
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(RS)-canadine + O2
berberine + H2O2
-
-
-
-
?
(RS)-tetrahydrocolumbamine + O2
?
-
-
-
-
?
(RS)-tetrahydropalmitine + O2
?
-
-
-
-
?
(RS)-tetrahydropapaverine + O2
?
-
-
-
-
?
(RS)-tetrahydropapaverine + O2
papaverine + H2O2
-
-
-
-
?
(S)-scoulerine + O2
?
-
DBOX does not fully aromatize the C-ring of (S)-scoulerine but instead catalyzes a two-electron oxidation
-
-
?
(S)-stylopine + O2
?
-
-
-
-
?
dihydrochelerythrine + O2
?
-
-
-
-
?
dihydrochelerythrine + O2
chelerythine + H2O2
-
-
-
-
?
dihydrochelerythrine + O2
chelerythrine + H2O2
-
-
-
-
?
dihydrochelirubine + O2
chelirubine + H2O2
-
-
-
?
Dihydromacarpine + O2
?
-
terminal step in the formation of benzophenanthridine alkaloids containing methylene dioxy substitutions in ring A and D
-
-
?
Dihydromacarpine + O2
Macarpine + H2O2
dihydrosanguinarine + O2
sanguinarine + H2O2
additional information
?
-
Dihydromacarpine + O2
Macarpine + H2O2
-
-
-
?
Dihydromacarpine + O2
Macarpine + H2O2
-
-
-
-
r
dihydrosanguinarine + O2
sanguinarine + H2O2
-
-
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
-
the enzyme catalyses the last reaction of sanguinarine formation
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
-
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
-
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
-
best substrate
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
-
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
-
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
-
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
-
last step in the biogenesis of sanguinarine
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
-
induced in a dose-dependent and time-dependent manner up to 4fold to 14fold, respectively when the cells are treated with jasmonate or with acetylsalicylic acid
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
?
additional information
?
-
-
the recombinant enzyme preferentially catalyzes the oxidation of dihydrosanguinarine to sanguinarine but also converts (RS)-tetrahydropapaverine to papaverine and several protoberberine alkaloids to oxidized forms, including (RS)-canadine to berberine
-
-
?
additional information
?
-
-
no reaction with: dihydronorsanguinarine, ajmalicine
-
-
?
additional information
?
-
-
one of the enzymes that catalyzes a later step in the biogenesis of benzo(c)phenanthridine alkaloids
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(RS)-canadine + O2
berberine + H2O2
-
-
-
-
?
(RS)-tetrahydrocolumbamine + O2
?
-
-
-
-
?
(RS)-tetrahydropalmitine + O2
?
-
-
-
-
?
(RS)-tetrahydropapaverine + O2
papaverine + H2O2
-
-
-
-
?
(S)-scoulerine + O2
?
-
DBOX does not fully aromatize the C-ring of (S)-scoulerine but instead catalyzes a two-electron oxidation
-
-
?
(S)-stylopine + O2
?
-
-
-
-
?
dihydrochelerythrine + O2
chelerythine + H2O2
-
-
-
-
?
Dihydromacarpine + O2
?
-
terminal step in the formation of benzophenanthridine alkaloids containing methylene dioxy substitutions in ring A and D
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
additional information
?
-
dihydrosanguinarine + O2
sanguinarine + H2O2
-
the enzyme catalyses the last reaction of sanguinarine formation
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
-
best substrate
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
-
last step in the biogenesis of sanguinarine
-
-
?
dihydrosanguinarine + O2
sanguinarine + H2O2
-
induced in a dose-dependent and time-dependent manner up to 4fold to 14fold, respectively when the cells are treated with jasmonate or with acetylsalicylic acid
-
-
?
additional information
?
-
-
the recombinant enzyme preferentially catalyzes the oxidation of dihydrosanguinarine to sanguinarine but also converts (RS)-tetrahydropapaverine to papaverine and several protoberberine alkaloids to oxidized forms, including (RS)-canadine to berberine
-
-
?
additional information
?
-
-
one of the enzymes that catalyzes a later step in the biogenesis of benzo(c)phenanthridine alkaloids
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
flavin
-
inhibition pattern indicates the presence of bound flavine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
copper
-
might be a copper metalloenzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1,4-DL-dithiothreitol
-
-
diethyldithiocarbamate
-
-
dicoumarin
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.146
(S)-scoulerine
-
pH 8.8, 37ưC
0.012
Dihydrochelerythrine
-
-
0.008
dihydrochelirubine
-
-
0.016 - 0.201
Dihydrosanguinarine
0.006
Dihydromacarpine
-
-
0.006
Dihydromacarpine
-
dihydrosanguinarine
0.016
Dihydrosanguinarine
-
-
0.201
Dihydrosanguinarine
-
pH 8.8, 37ưC
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
-
additional information
-
-
additional information
-
sensitive, rapid and convenient fluorimetric assay
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.5 - 8
-
about 50% of maximal activity at pH 5.5 and 8.0
6 - 8.5
-
about 50% of maximal activity at pH 6.0 and 8.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
very low level compared to roots
brenda
-
very low level compared to roots
brenda
-
predominantly
brenda
-
-
brenda
-
very low level compared to roots
brenda
additional information
-
enzyme expression patterns, overview
brenda
-
-
brenda
-
elicited cell suspension
brenda
-
elicited cell suspension
brenda
-
elicited tissues
brenda
-
-
brenda
-
elicited cell suspension
brenda
-
elicited cells
brenda
-
elicited tissues
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
malfunction
-
virus-induced gene silencing to reduce DBOX transcript levels results in a corresponding reduction in sanguinarine, dihydrosanguinarine, and papaverine accumulation in opium poppy roots
metabolism
-
DBOX as a multifunctional oxidative enzyme in benzylisoquinoline alkaloid metabolism, overview. The two-electron oxidation of dihydrosanguinarine catalyzed by dihydrobenzophenanthridine oxidase is the final step in sanguinarine biosynthesis. The formation of the fully conjugated ring system in sanguinarine is similar to the four-electron oxidations of (S)-canadine to berberine and (S)-tetrahydropapaverine to papaverine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
77000
-
x * 77000, SDS-PAGE, the lower MW bands include enzymes of the polyphenol oxidase family that copurify with dihydrobenzophenanthridine oxidase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 77000, SDS-PAGE, the lower MW bands include enzymes of the polyphenol oxidase family that copurify with dihydrobenzophenanthridine oxidase
?
-
a major component of MW 77000 and two minor components with MW between 59000 and 67000 are detected by SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
baculo virus-induced gene silencing in planta using Agrobacterium tumefaciens strain GV3101 transfection to reduce DBOX transcript levels, real-time quantitative PCR expression analysis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time quantitative PCR expression analysis, expression in Pichia pastoris
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
enzyme activity increases in the yeast extract-exposed cultures, but not in the methyl jasmonate-exposed cultures. There is a slight increase in activity during the first 24 h, whereas maximal response occurred at 48 h and it was maintained for up 72 h
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ignatov, A.; Clark, W.G.; Cline, S.D.; Psenak, M.; Krueger, R.J.; Coscia, C.J.
Elicitation of dihydrobenzophenanthridine oxidase in Sanguinaria canadensis cell culture
Phytochemistry
43
1141-1144
1996
Sanguinaria canadensis
brenda
Schumacher, H.M.; Zenk, M.H.
Partial purification and characterization of dihydrobenzophenanthridine oxidase from Eschscholtzia californica cell suspension cultures
Plant Cell Rep.
7
43-46
1988
Eschscholzia californica
brenda
Arakawa, H.; Clark, W.G.; Psenak, M.; Coscia, C.J.
Purification and characterization of dihydrobenzophenanthridine oxidase from elicited Sanguinaria canadensis cell cultures
Arch. Biochem. Biophys.
299
1-7
1992
Sanguinaria canadensis
brenda
Psenak, M.
A fluorimetric assay of dihydrobenzophenanthridine oxidase
Biologia (Bratisl. )
46
1109-1112
1991
Papaver somniferum, Sanguinaria canadensis
-
brenda
Ignatov, A.; Neuman, M.C.; Barg, R.; Krueger, R.J.; Coscia, C.J.
Immunoblot analyses of the elicited Sanguinaria canadensis enzyme, dihydrobenzophenanthridine oxidase: evidence for resolution from a polyphenol oxidase isozyme
Arch. Biochem. Biophys.
347
208-212
1997
Sanguinaria canadensis
brenda
Hagel, J.M.; Beaudoin, G.A.; Fossati, E.; Ekins, A.; Martin, V.J.; Facchini, P.J.
Characterization of a flavoprotein oxidase from opium poppy catalyzing the final steps in sanguinarine and papaverine biosynthesis
J. Biol. Chem.
287
42972-42983
2012
Papaver somniferum
brenda
Guizar-Gonzalez, C.; Monforte-Gonzalez, M.; Vazquez-Flota, F.
Yeast extract induction of sanguinarine biosynthesis is partially dependent on the octadecanoic acid pathway in cell cultures of Argemone mexicana L., the Mexican poppy
Biotechnol. Lett.
38
1237-1242
2016
Argemone mexicana
brenda
Select items on the left to see more content.
html completed